Molecular cloning and functional characterization of peptidoglycan recognition protein 6 in grass carp Ctenopharyngodon idella

Peptidoglycan recognition proteins (PGRPs) are pattern recognition molecules of innate immunity. In this study, a long-form PGRP, designated as gcPGRP6, was identified from grass carp Ctenopharyngodon idella. The deduced amino acid sequence of gcPGRP6 is composed of 464 residues with a conserved PGRP domain at the C-terminus. The gcPGRP6 gene consists of four exons and three introns, spacing approximately 2.7 kb of genomic sequence. Phylogenetic analysis demonstrated that gcPGRP6 is clustered closely with zebrafish PGLYRP6, and formed a long-type PGRP subfamily together with PGLYRP2 members identified in teleosts and mammals. Real-time PCR and Western blotting analyses revealed that gcPGRP6 is constitutively expressed in organs/tissues examined, and its expression was significantly induced in liver and intestine of grass carp in response to PGN stimulation and in CIK cells treated with lipoteichoic acid (LTA), polyinosinic polycytidylic acid (Poly I:C) and peptidoglycan (PGN). Immunofluorescence microscopy and Western blotting analyses revealed that gcPGRP6 is effectively secreted to the exterior of CIK cells. The over-expression of gcPGRP6 in CIK cells leads to the activation of NF-κB and the inhibition of intracellular bacterial growth. Moreover, cell lysates from CIK cells transfected with pTurbo-gcPGRP6-GFP plasmid display the binding activity towards Lys-type PGN from Staphylococcus aureus and DAP-type PGN from Bacillus subtilis. Furthermore, proinflammatory cytokine IL-2 and intracellular PGN receptor NOD2 had a significantly increased expression in CIK cells overexpressed with gcPGRP6. It is demonstrated that the PGRP6 in grass carp has a role in binding PGN, in inhibiting the growth of intracellular bacteria, and in activating NF-κB, as well as in regulating innate immune genes.     

Interrelationship among muscle,fat, and bone: Connecting the dots on cellular,hormonal, and whole body levels

While sarcopenia and sarcopenic obesity have been recognized in the last decade, a combined concept to include decreased muscle mass and strength, as well as decreased bone mass with coexistence of adiposity is discussed here. We introduce a new term, osteopenic obesity, and operationalize its meaning within the context of osteopenia and obesity. Next, we consolidate osteopenic obesity with the already existing and more familiar term, sarcopenic obesity, and delineate the resulting combined condition assigning it the term osteosarcopenic obesity. Identification and possible diagnosis of each condition are discussed, as well as the interactions of muscle, fat and bone tissues on cellular level, considering their endocrine features. Special emphasis is placed on the mesenchymal stem cell commitment into osteoblastogenic, adipogenic and myogenic lineages and causes of its deregulation. Based on the presented evidence and as expounded within the text, it is reasonable to say that under certain conditions, osteoporosis and sarcopenia could be the obesity of bone and muscle, respectively, with the term osteosarcopenic obesity as an encompassment for all.     

HBV感染慢性肝病患者血清GP73浓度对肝纤维化程度的诊断意义

目的 探讨血清GP73浓度与慢性HBV肝病患者肝纤维化程度之间的关系,以评估GP73在慢性HBV患者中的临床诊断价值.方法 选取慢性HBV感染者761名,分别进行FibroScan检测和血清GP73测试.结果 血清GP73浓度与肝硬度相关（r=0.601）,ROC曲线下面积为0.76.GP73用于诊断显著肝纤维化（≥F2）的敏感性和特异性分别为62.81％和80.05％（cut off值：76.6ng/ml）.结论 GP73可能是一种诊断慢性HBV感染者显著肝纤维化的新标志物.     

珊瑚骨粉复合组织补片进行拔牙位点保存的临床研究

目的探讨使用珊瑚骨粉复合组织补片进行拔牙位点保存的临床效果。方法选取自2012年12月至2014年2月期间在武警总医院口腔种植科门诊采用微创技术拔除磨牙的患者45例,试验组20例,拔牙窝内植入珊瑚骨粉,组织补片覆盖表面关闭拔牙窝;对照组25例,拔牙后常规处理。1、3、6个月复诊,观察拔牙窝愈合情况。拔牙前及拔牙6个月后进行口内取模灌注石膏模型并拍摄X线牙片,分别测量牙槽嵴的高度和宽度,使用配对t检验分别对两组拔牙前至6个月期间牙槽嵴高度、宽度的变化进行统计学分析,使用两独立样本的t检验进行两组间牙槽嵴高度、宽度变化的比较,P〈0.05为差异具有统计学意义。结果临床观察除有一例组织补片脱落外,试验组其他拔牙窝均愈合良好,牙槽骨丰满,牙龈色、形、质与邻牙协调;而对照组牙槽嵴吸收、萎缩,高度降低、宽度显著缩小。统计学分析试验组牙槽嵴高度和宽度的改变无显著的统计学差异（P〉0.05）;对照组的上述改变具有统计学差异（P〈0.05）。两组间牙槽嵴高度、宽度的变化相比,二者间的差异均具有统计学意义（P〈0.05）。结论珊瑚骨粉复合组织补片有效保存了进行种植牙修复所必需的骨量,是进行拔牙后位点保存术的合适材料。     

Chronic progressive external ophthalmoplegia with inflammatory myopathy

Chronic progressive external ophthalmoplegia is one of mitochondrial disorders, characterized by ptosis, limitation of eye movement, variably severe bulbar muscle weakness and proximal limb weakness. Chronic progressive external ophthalmoplegia complicated with acquired disease is extremely rare. We report a 44 years old male patient with more than 20 years of chronic progressive bilateral ptosis and limitation of eye movements manifested dysarthria, dysphagia and neck muscle weakness for 3 years. The first muscle biopsy showed red-ragged fibers and cytochrome c oxidase negative fibers as well as inflammatory cells infiltration. Electron microscopy revealed paracrystalline inclusions. Mitochondrial genetic analysis demonstrated a large-scale mtDNA deletion of m.8470_13446del4977. The patient was treated with prednisone. In a three-year follow-up study, the second biopsy was performed. Before the treatment, except bilateral ptosis and external ophthalmopelgia, this patient presented bulbar muscle weakness and neck muscle weakness. After treated with prednisone, the symptoms of dysphagia, dysarthria and neck muscle weakness were significantly improved, and the second biopsy showed only mitochondrial myopathy pathology but the inflammations disappeared. Here, we report a patient with chronic progressive external ophthalmoplegia complicated with inflammatory myopathy and after treated with prednisone as myositis, he had a significant therapeutic effect.     

1例新型冠状病毒肺炎危重症患者人工气道的管理

目的总结新型冠状病毒肺炎(以下简称新冠肺炎)危重症患者人工气道的管理经验。方法2019年1月28日,某院收治了1例新冠肺炎危重症患者,予紧急有创机械通气治疗。结果经过积极治疗9 d,患者病情逐渐平稳,予试脱机试验。结论人工气道建立前的评估准备、个人防护要点,建立时的注意事项及建立后的气道管理工作的经验,可为以后收治烈性传染病提供参考。     

CDCA3在食管癌中的表达及其对食管癌细胞增殖与凋亡的影响

目的研究细胞周期相关因子(CDCA3)在食管癌组织和细胞中的表达及其对食管癌细胞增殖与凋亡的影响。方法实时荧光定量PCR(qPCR)检测50例食管癌组织及其配对的癌旁组织中CDCA3 mRNA的表达水平,同时检测CDCA3 mRNA在不同食管癌细胞系(Eca109、Kyse-170、TE1)和人食管鳞状上皮细胞系Het-1A中的表达水平;采用si-CDCA3-1、si-CDCA3-2转染Eca109和TE1细胞,另转染si-NC作为阴性对照,MTS法检测细胞增殖能力,流式细胞仪检测细胞周期和凋亡变化;Western blot检测CDCA3、CyclinD1和cleaved caspase-3蛋白的表达变化。结果食管癌组织中CDCA3 mRNA的表达高于癌旁组织(P<0.05),Eca109、Kyse-170和TE1细胞中CDCA3 mRNA表达高于人食管鳞状上皮细胞(P<0.05)。沉默CDCA3表达后,Eca109细胞和TE1细胞的增殖率降低,G0/G1期细胞比例增加(P<0.05)。沉默CDCA3表达后,Eca109细胞和TE1细胞的凋亡率增加(P<0.05)。沉默CDCA3表达可降低CyclinD1蛋白表达和增加cleaved caspase-3蛋白表达(P<0.05)。结论CDCA3 mRNA在食管癌组织和细胞系中均高表达,干扰CDCA3的表达可能通过降低CyclinD1蛋白表达抑制细胞增殖,增加cleaved caspase-3蛋白表达促进细胞凋亡,提示CDCA3可能为食管癌的潜在治疗靶标。     

高声压脉冲超声空化作用对兔肝细胞水通道蛋白4及钠钾ATP酶表达的影响

目的 探讨高声压脉冲超声空化作用对兔肝细胞水通道蛋白4(AQP 4)及钠钾ATP酶(Na+-K+ATPase)表达的影响.方法 将健康新西兰大白兔41只随机分为空白对照组(n=5)及不同辐照时间组:T1组(辐照时间1 min,n=12)、T5组(辐照时间5 min,n=12)及T10组(辐照时间10 min,n=12)...     

细胞因子在白血病异基因造血干细胞移植后移植物抗宿主病中的水平及其意义

目的:探究细胞因子TNF-α、IL-6和IL-8在白血病异基因造血干细胞移植后的变化和病情及预后的关系.方法:用放射免疫分析检测了白血病异基因造血干细胞移植患者(48例)、正常健康人(30例)血清TNF-α、IL-6和IL-8水平,对移植后发生或未发生急性移植物抗宿主病(aGVHD)及发生或未发生慢性移植物抗宿主病(cGVHD)进行了比较,并对预后也进行比较.结果:急、慢性GVHD组与未发生急、慢性GVHD组患者的血清TNF-α、IL-6及IL-8水平均高于正常对照组(P＜0.05,除未发生cGVHD组的TNF-α浓度低外);急、慢性GVHD组与未发生急、慢性GVHD组比血清TNF-α、IL-6及IL-8水平均升高(P＜0.05);移植后死亡组生存时间≤6月及＞6月(除＞6月的IL-8水平外)各组TNF、IL-6与IL-8水平均高于同期的存活组(P＜0.05).结论:异基因造血干细胞移植术后动态监测TNF、IL-6、IL-8水平变化有助于对发生aGVHD 或cGVHD的可能提供另一种检测方法,并对判断愈后有一定的临床意义.