RIG-Ⅰ与干扰素信号通路的关系

目的研究干扰素(IFNs)对维甲酸诱导Ⅰ型基因(RIG-Ⅰ)的调控作用,同时探讨RIG-Ⅰ对IFNs生物学效应的介导作用。方法Northern杂交和半定量RT-PCR检测小鼠胚胎成纤维细胞(MEFs)、在IFNs作用前后RIG-Ⅰ基因的表达。MTT比色法和细胞病变抑制法比较野生型和RIG-基因剔除(RIG-Ⅰ-/-)小鼠的MEFs在IFNs作用前后的生长情况和抗病毒活性。结果IFN-γ、IFN-β均能上调小鼠MEFs中RIG-Ⅰ的表达;100 U/mL IFN-γ处理后,野生型小鼠MEFs的生长抑制较RIG-Ⅰ-/-小鼠的MEFs明显;RIG-Ⅰ缺失后,IFN-β对细胞病毒感染的保护作用较野生型明显减弱。结论RIG-Ⅰ是一新发现的介导干扰素细胞效应的基因,它可能通过调节细胞因子的产生参与干扰素介导的炎症反应。     

辅助抗生素治疗宫颈癌前病变并发幽门螺杆菌感染的疗效观察

①目的探讨辅助抗生素治疗对宫颈癌前病变并发幽门螺杆菌（Hp）感染的治疗效果。②方法采用快速Hp尿素酶检测法筛选宫颈癌前病变的相关病例，用阴道镜和组织病理学进行诊断证实。对各类宫颈癌前病变创伤性治疗的同时，规范采用甲硝唑治疗。观察幽门螺旋杆菌阳性率的改变和临床表现的变化。③结果Hp感染与宫颈癌前病变发展为宫颈癌有相关性；除了手术治疗外，规范的甲硝唑治疗可以降低Hp感染的阳性发生率，同时，可以对宫颈癌起病因学治疗作用。④结论辅助抗生素治疗对宫颈癌前病变并发Hp感染的患者．可以起到消除潜在癌变的诱因和提高疗效的作用。     

“No jobs,more crime. More jobs,less crime”: structural factors affecting the health of Latino men in Detroit

BackgroundLatinos comprise 14% of the United States (U.S.) population, and approximately 75% of Latinos in the U.S. are immigrants or children of immigrants. Urban Latino men face a distinct set of structural barriers that may influence their health and well-being. The collapse of the automobile industry in Detroit, Michigan, contributed to increased rates of racially stratified unemployment.MethodThrough semi-structured interviews and ethnography, we present a case study of nine Latino men who face decreasing opportunities for formal employment and navigate the constant threat of violence and deportation in the face of Detroit's rapid deindustrialization.ResultsIn light of scarce work opportunities, participants sought income from the informal labor sector. This work was often illegal or dangerous, such as drug dealing, and perpetuated a cycle of violence. The threat of violence and deportation limited mobility and contact with authorities, especially among undocumented participants.ConclusionIn order to promote the health of urban Latino men, a careful consideration of the structural factors that shape their lives is essential. Interventions should work to leverage an extensive skill set and drive to work into meaningful formal employment, and consider the overarching effects of documentation on social mobility.