Neck design对牙根形种植体边缘骨吸收的影响

目的：评价种植体功能性负载后对种植体表面在边缘骨水平的宏观和微观结构的影响。方法：采用非嵌入式种植一期手术,不行引导骨再生术。种植术后按时间段（3,6,12个月）进行影像学观察。结果：Ankylos种植系统（1.32±0.24mm）3个时段出现轻微的骨吸收,有统计学意义（P〈0.05）。Straumann种植系统（0.76±0.19mm）与Osstem种植系统组（0.21±0.12mm）3个月时段骨吸收量显著减少,但3～6个月与6～12月时段骨吸收量无显著性差异（P〉0.05）。3个种植系统组在上、下颌植入部位的边缘骨吸收量无统计学意义（P〉0.05）。结论：不同颈部设计的牙根形种植体边缘骨吸收量具有显著性差异,宏观表面结构种植体系统比精密颈部与微观表面结构的种植系统边缘骨吸收更稳定。     

豌豆中硒的氢化物发生—原子荧光光谱测定法

目的建立氢化物发生—原子荧光光谱法测定豌豆中硒的含量。方法以HNO3-HClO4消解样品,用HCl将6价硒还原为4价硒在选定的最佳实验条件下进行测定。结果方法的检出限为0.10ng/ml,线性范围为0～80ng/ml。应用于3种豌豆中硒的测定,回收率为90.5%～110.6%,RSD为2.0%～5.7%。结论该方法样品处理简单,检测速度快,一般实验室均可应用。     

高效液相色谱法测定犬血浆中丹参素钠

目的 建立了紫外检测的快速灵敏的高效液相色谱法,用于测定犬血浆中的丹参素钠。方法 血浆样品经液-液萃取后,以原儿茶酸为内标,采用Discovery C₁₈色谱柱进行分离,HPLC测定犬血浆中的药物浓度。结果 犬分别静脉滴注给予10、20、40 mg/kg丹参素钠后,AUC_0-t、AUC_0-∞、C_max均与给药剂量呈线性相关。AUC_0-t、AUC_0-∞的相关系数分别为0.964、0.964。C_max的相关系数为0.934 7;丹参素钠在犬体内的药动学过程符合二室模型。结论 通过方法学确证,该方法可以应用于丹参素钠在犬血浆中的药物动力学研究。     

Hcy、NSE及IL-2与缺血性脑卒中急性期患者认知功能的相关性研究

目的　探讨同型半胱氨酸（Hcy）、神经元特异烯醇化酶（NSE）及白细胞介素-2（IL-2）与缺血性脑卒中急性期患者认知功能的相关性,为减轻认知功能损伤提供指导。方法　将烟台业达医院神经内科2019年4月至2021年5月接收的缺血性脑卒中急性期（发病后1～2周）患者122例作为研究对象,其中男70例、女52例,年龄（66.67±9.16）岁。采用简易智力状态检查量表（MMSE）评测其认知功能,采集空腹静脉血检测Hcy、NSE及IL-2水平,并完成基线资料调查;对比不同认知功能组患者的基线资料、Hcy、NSE及IL-2水平。Pearson相关性分析Hcy、NSE及IL-2与MMSE评分的相关性;线性回归分析MMSE评分的影响因子。计数资料采用χ²检验,计量资料多组间比较采用单因素方差分析、两两比较采用LSD法。结果　122例缺血性脑卒中急性期患者根据MMSE评分被分为认知功能正常组（65例）、轻度认知功能障碍组（33例）、中度认知功能障碍组（15例）、重度认知功能障碍组（9例）。轻度认知功能障碍组、中度认知功能障碍组及重度认知功能障碍组的Hcy、NSE及IL-2水平均高于认知功能正常组（均P<0.05）;重度认知功能障碍组的Hcy、NSE水平均高于轻度认知功能障碍组［（29.49±8.64）µmol/L比（23.33±5.17）µmol/L、（33.25±8.32）ng/L比（24.28±6.12）ng/L］,差异均有统计学意义（均P<0.05）。Hcy、NSE、IL-2与MMSE评分均呈显著负相关性,相关系数分别为-0.577、-0.549、-0.498,均P<0.001。线性回归分析显示年龄、Hcy、NSE、IL-2均是缺血性脑卒中急性期患者MMSE评分的影响因子（均P<0.001）。结论　Hcy、NSE、IL-2与缺血性脑卒中急性期患者MMSE评分呈负相关性,年龄、Hcy、NSE、IL-2与缺血性脑卒中认知功能损伤有关,调控Hcy、NSE、IL-2水平可能有助于减轻其认知功能损伤。     

杭白菊提取物中辅料麦芽糊精的中红外快速定量分析

目的建立一种对杭白菊提取物中麦芽糊精含量进行快速定量的中红外分析方法。方法以78份不同麦芽糊精含量的杭白菊提取物为实验对象,对采集到的已知麦芽糊精含量的样品谱图进行图谱预处理,以偏最小二乘法建立含量测定模型。结果所建含量测定模型的决定系数R²为0.9987,预测标准差为1.137。结论所建含量测定模型测定效果较好、稳定性强、检测精度高,可用于杭白菊提取物中麦芽糊精含量的快速分析。     

磁共振表观扩散系数直方图和血清CA125有助于评估子宫内膜样腺癌的病理组织学特征

  目的   探究基于子宫内膜样腺癌表观扩散系数(ADC)直方图分析和糖类抗原125(CA125)与组织分化程度、微卫星、Ki-67的相关性。   方法   搜集68例首诊且经病理证实为子宫内膜样腺癌患者资料。所有患者均行超导核磁共振扫描,用FireVoxel软件在ADC图测得肿瘤感兴趣区的直方图参数。分析子宫内膜样腺癌的直方图参数和CA125与组织分化程度、微卫星、Ki-67的相关性。   结果   直方图参数、Ki-67差距在不同组织分化程度中均有统计学意义(P < 0.05)。直方图参数、Ki-67及CA125均值差距在微卫星稳定组与不稳定组没有统计学意义(P>0.05)。ROC曲线统计计算出P90值对应的曲线下面积最大(0.937)。直方图参数与组织分化程度呈低或中等程度负相关(P < 0.05);平均数、最大值、P10、P25、P50、P75、P90、P95与Ki-67呈低等程度负相关(P < 0.05)。   结论   ADC直方图参数和血清CA125有助于评估子宫内膜腺癌的病理组织学特征。      

Metabolomics comparison of metabolites and functional pathways in the gills of Chlamys farreri under cadmium exposure

The biological processes of Chlamys farreri (C. farreri), an economically important shellfish, are affected when exposed to Cd²⁺. In this study, changes to biological processes and metabolite levels in C. farreri were examined when exposed to Cd²⁺. Ultra-performance liquid chromatography-tandem TOF mass spectrometry (UPLC-TOF/MS)-based untargeted metabolomics was used to examine changes in the metabolism of C. farreri gill tissue exposed to 0.050 mg/L Cd²⁺ for 96 h in a natural environment. Sixty-eight metabolites with significant differences were screened by multivariate statistical analysis. Eleven enriched functional pathways displayed significant changes in inactivity. Differential metabolites, mainly C00157 and C00350, have a significant impact on functional pathways and can be used as potential major biomarkers. Lipid phosphorylation, disruption of signal transduction, and autophagy activation were observed to change in C. farreri when exposed to Cd. The metabolome information supplements research on C. farreri exposure to heavy metals and provides a platform for further multi-omics analysis.     

Studies on the chemical constituents of Valeriana fauriei Briq

Nine compounds were isolated from the roots of Valeriana fauriei Briq, of which one is a new germacrane-type sesquiterpenoid named as valerianin E and its structure was elucidated as bicyclo[8, 1, 0] 5β-hydroxyl-7β-acetoxyl-5α,11, 11′-trimethyl-E-1(10)–ene-4α, 15-olide (1). In addition, two were first reported from this genus and the others were isolated for the first time from the title plant.     

Daphnoretin induces reactive oxygen species-mediated apoptosis in melanoma cells

Research suggests that daphnoretin exhibits a diverse array of antitumor mechanisms and pharmacological activities. However, there is no definitive explanation for the antitumor mechanisms of daphnoretin in malignant melanoma. In the present study, MTT and colony formation assays demonstrated that daphnoretin significantly inhibited the proliferation of melanoma A375 and B16 cells. Following treatment with daphnoretin, apoptotic bodies were observed in A375 and B16 cells via Hoechst 33258 staining. Furthermore, western blot analysis revealed that the apoptosis-related proteins cleaved caspase-3, cleaved caspase-9, Bax, cytochrome c and apoptotic protease-activating factor 1 were significantly upregulated, while the expression levels of caspase-3, caspase-9 and Bcl-2 were downregulated in A375 and B16 cells. Flow cytometry and fluorescence microscopy revealed that daphnoretin induced higher levels of reactive oxygen species (ROS). Therefore, the results of the present study indicated that daphnoretin induced ROS-mediated mitochondria apoptosis in human (A375) and murine (B16) malignant melanoma cells.