趋化因子受体CXCR4小分子干扰RNA表达载体的构建及表达

目的： 构建趋化因子受体CXCR4小分子干扰RNA（siRNA）表达载体，研究其对CXCR4基因表达的沉默效果，探索抗人免疫缺陷病毒-1 （HIV-1）治疗的新途径。方法： 根据软件设计针对CXCR4基因的siRNA序列，经退火成互补双链，克隆到Psilencer^M3.1-H1 neo载体中，经测序鉴定插入序列的正确性。转染MT₄细胞后，采用RT-PCR及流式细胞仪方法检测转染siRNA质粒的实验组、转染空载体的阴性对照组和空白细胞对照组间抑制基因表达情况。结果： PCR及琼脂糖凝胶电泳检测重组质粒Psilencer^M 3.1-H1- siRNA neo，有正确的特异性片段，DNA测序也证明具有正确序列；该载体转染MT₄细胞48 h后，能明显抑制CXCR4基因表达，其表达抑制率为70%，与空白对照组和阴性组比较差异有显著性（P<0.05）。结论： 成功构建趋化因子受体CXCR4的siRNA表达载体。     

综合治疗非淋菌性尿道炎合并前列腺炎疗效观察

目的评价综合治疗非淋菌性尿道炎合并前列腺炎的疗效和不良反应。方法将患者随机分成两组进行开放性对比研究观察，治疗组120例用敏感抗生素、微波，中成药及外用药治疗；对照组112例，单用敏感抗生素治疗，两组均3周为一疗程，停药1周后复查评价疗效。结果治疗组有效率（90％）明显高于对照组（50％），两者有非常显著性差异（P〈0．001）。两组均未发现肝肾功能损害，不良反应轻微。结论综合治疗非淋菌性尿道炎合并前列腺炎效果显著。     

A novel tylophorine analog NK-007 ameliorates colitis through inhibition of innate immune response

In this study, we synthesized (±)-tylophorine malate (NK-007), an analog of tylophorine (DCB3503), and analyzed its anti-inflammatory effect in vivo using a dextran sulfate sodium (DSS)-induced colitis model and an acetic acid-induced colitis model. As indicated by disease activity index (DAI) and degree of macroscopic colonic damage, NK-007 can significantly suppress colitis. To delineate the underlying mechanism, we have explored the influence of NK-007 on the production of TNF-α by murine primary bone marrow-derived dendritic cells (BMDCs) as well as monocyte/macrophage cell line Raw 264.7 triggered by lipopolysaccharide (LPS). For both types of innate immune cells, NK-007 showed a potent TNF-α inhibitory effect, and has in addition reduced the expression of IL-12 in BMDCs. Moreover, Raw cells treated with NK-007 also showed decreased phosphorylation of NF-κB, which may explain the protective immune-regulatory effect of NK-007 for experimental colitis.     

Polychlorinated Biphenyls in the Atmosphere of an Urban City: Levels,Distribution, and Emissions

Polychlorinated biphenyl (PCB) concentrations, profiles, and possible sources were determined in the atmosphere of Guangzhou, the largest city in south China. ∑PCB concentrations ranged from 160 to 2720 pg/m³, which is comparable with values found by similar studies in North America, Europe, and Asia. The highest PCB concentrations were found in the old industrial district, suggesting it to be the principal emission source. The most important PCB homologue group was tetra-PCB, followed by tri- and penta-PCB. The PCBs’ homologue composition differs from that found in Chinese transformer oils: Chinese PCB products (no. 1 PCB and no. 2 PCB), Aroclor1242, and Aroclor1254. However, it is similar in composition to that found in sediments and soils subjected to arbitrary disposal of used electronic appliances in this region. Our results suggest that volatilization from PCB-contaminated soils in the old urban center may be the major source of PCBs in the atmosphere of Guangzhou. Additional studies will be required to characterize the geochemical cycles of PCBs from the contaminated environmental “hot spots” during the typical subtropical climate conditions in the study regions.     

Estimated PBDE and PBB Congeners in Soil from an Electronics Waste Disposal Site

This study estimated PBDE and PBB congener emissions into the environment from three different e-waste disposal sites that consisted of rude broilers (RB), acid baths (AB) and end-up dumps (ED). Different PBDE and PBB congener profiles were analyzed, and some of their emission sources were discussed. For the examined sites, the levels of ΣPBB and ΣPBDE were highest at ED (ΣPBB 1943.86 ng/g, dw; ΣPBDE 990.87 ng/g, dw), followed by RB (ΣPBB 704.58 ng/g, dw; ΣPBDE 799.27 ng/g, dw) and finally AB (ΣPBB 108.78 ng/g, dw; ΣPBDE 171.18 ng/g, dw). PBE-209 (AB 48.67 ng/g, 28.43%; RB 160.23 ng/g, 20.05%; ED 234.12 ng/g, 23.63%) and PBE-203 (AB 51.23 ng/g, 29.93%; RB 130.34 ng/g, 16.31%; ED 93.41 ng/g, 9.43%) exhibited the highest concentrations when compared to the other congeners. Furthermore, high levels of deca-BDE (BDE-209) were observed in AB (48.67 ng/g, 28.43%) and RB sites (160.23 ng/g, 20.05%), which was in accordance with the large amount of these materials in electronics products in China.     

脉络膜新生血管性病变研究现状

脉络膜新生血管性病变主要见于年龄相关性黄斑变性和中心性渗出性脉络膜视网膜病变等眼底病,是导致视力减退甚至丧失的主要原因之一。本文回顾了近年来国内外与脉络膜新生血管性病变有关的疾病病因与发病机理,临床表现与分类,检查方法和治疗手段等研究成果,并展望中药在治疗脉络膜新生血管性病变的美好前景。     

Environmental DNA: An Emerging Tool in Ecological Assessment

Bulletin of Environmental Contamination and Toxicology - Environmental DNA (eDNA), as a recent research hotspot in environmental science, the use of eDNA in biological monitoring has the advantages...     

恶性疟原虫染色体基因文库的构建

恶性疟原虫FCC1/HN株DNA经Bam HI部分消化后,取17-22kb片段插入到载体EMBL_4DNA左右臂之间,经体外包装后,感染P_2溶源菌E.coli L_(95),建成基因文库,使原虫基因组中的每一段DNA序列均有99％的几率克隆到文库中。通过原位杂交,筛出了含有重复序列的特异克隆,证明该库稳定而有效。