Differentiation of Swertia Mussotii Franch from Artemisiae Capillaris Herba by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis (CE) with electrochemical detection (ED) method is developed for differentiation of Swertia Mussotii Franch from Artemisiae Capillaris Herba in this work. Swertia Mussotii Franch contains a great deal of swertiamarin and mangiferin that are not present in Artemisiae Capillaris Herba, whereas Artemisiae Capillaris Herba consists of abundant chlorogentic acid. Therefore, determining their swertiamarin, mangiferin and chlorogentic acid contents can differentiate these two crude herbs. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits good response at +1000 mV (versus SCE) for the three analytes. With a separation voltage of 14 kV, the three analytes were separated within 14 min in a 52 cm length capillary in 50 mmol/l borax buffer (pH 9.2). The system was demonstrated good stability and reproducibility with an R.S.D. of less than 5% for both migration time and peak current. This method was successfully used to analyze and identify the crude herbs with satisfactory assay results.     

The glomerulonephritides

The glomerulonephritides are a group of conditions characterized by inflammation primarily within the renal glomerulus. The main clinical features are haematuria, hypertension and reduced renal function. Oedema and proteinuria are common. The severity varies from asymptomatic to catastrophic renal failure. Careful evaluation is required in order accurately to determine the correct diagnosis and treatment.     

超临界CO2萃取迷迭香中抗氧化活性成分的优化工艺研究

目的:研究迷迭香中抗氧化活性成分的萃取工艺。方法:设计4因素3水平正交试验,利用超临界CO₂流体萃取技术,结合电子顺磁共振(EPR)技术和自旋捕集技术检测各萃取物清除羟自由基的性能,以自由基清除率为指标,通过极差和方差分析,研究萃取迷迭香抗氧化活性成分的优选工艺条件。结果与结论:迷迭香抗氧化成分的优选萃取工艺为:夹带剂用量0.3 mL.g^-1,萃取时间1 h,萃取压力30 MPa,萃取温度75℃。