Prof. Shan Zhaowei＇s Experience in Treating Atrophic Gastritis

Prof. Shan Zhaowei （ 单兆伟 ）, the author＇s teacher, has been engaged in TCM for more than 30 years, with profound knowledge in the treatment of diseases of the spleen and stomach, which are introduced in the following.     

Clinical Observation on the Method of Supplementing Qi, Clearing away Heat and Promoting Blood Circulation for Treating 53 Cases of Gastritis Related to Pyrolic Helicobacterium

According to the clinical experience of professor Shan Zhaowei (单兆伟), the author has successfully developed Qingyou Yangwei Capsule (清幽养胃胶囊capsules for eliminating pyrolic Helicobacterium and nourishing the stomach) for treating the gastritis. Clinical research has shown that the capsule has a good curative effect on chronic gastritis related to pyrolic Helicobacterium. Among the 53 cases in the treatment group, clinical observation showed cure in 6 cases, obvious effect in 18 cases, effect in 24 cases, no effect on 5 cases, with a total effective rate of 90.5%. Of the 50 cases in the control group, cure was found in 5 cases, obvious effect in 13 cases, effect in 20 cases, no effect in 12 cases, the total effective rate being 76%. The total effective rate in the treatment group was higher than that in the control group. The rate of eliminating pyrolic Helicobacterium in the treatment group was 66.04%, similar to that in the control group. In a follow-up visit 6 months later, the recurrence rate in the treatment group was 13.33%, obviously lower than that of 41.66% in the control group.     

帕米磷酸钠和降钙素对人工关节磨损微粒引起单核细胞分泌溶骨性因子的影响

目的　探讨帕米磷酸钠和降钙素防治人工关节松动的可能性。方法　采集人体外周血 ,分离单核细胞 ,分组培养。实验分 4组 ,第 1组 :仅单核细胞 ,为对照组 ;第 2组 :单核细胞及微粒 ,为微粒组 ;第 3组 :单核细胞、微粒及帕米磷酸钠 (阿可达 ) ,为阿可达组 ;第 4组 :单核细胞、微粒及降钙素 (密钙息 ) ,为密钙息组。培养 48h后 ,检测细胞上清液中肿瘤坏死因子 (TNF) α、白细胞介素 (IL) 1和IL 6的含量。结果　微粒组的细胞上清液中溶骨性因子含量明显高于对照组 (P <0 .0 1) ;而阿可达组和密钙息组的溶骨性因子含量明显低于微粒组 (P <0 .0 1)。结论　阿可达和密钙息能有效地抑制微粒刺激单核巨噬细胞分泌溶骨性因子 ,从而间接抑制破骨细胞的活性。     

脑肿瘤中p53基因突变的分析

检测２５例脑肿瘤中抑制癌基因Ｐ５３突变情况。突用复合ＰＣＲ，聚合酶链反应－＝单链构象多态性分析法，统计学处理采用Ｘ＾２检验。结果：Ｐ５３基因总的突变率为４４．０％，其中第５外显子突变率为２８．０％，第６外显子突变率１２．０％，第７外显子突变率为１２．０％，第８外显子突变率１６．０％；病理分级Ⅱ级及其以上突变为７２．７％，     

Acupuncture Treatment for 34 Cases of Epiphora with Dysfunction of Lacrimal Duct

We have treated 34 cases of epiphora due to dysfunction of the lacrimal duct with a warm needle puncturing at the Jingming (UB 1) point. The effect was significantly better than that of the control group treated with lacrimal duct irrigation and norflaxacin eye drops (a total effective rate of 91.07% vs. 54.29%).     

The Effect of Acupuncture on Bone Mineral Density in Postmenopausal Women

According to the TCM theory that the kidney is in charge of the bone, the authors carried out a study on the effect of acupuncture on bone mineral density in 40 postmenopausal women with osteoporosis treated by the method of reinforcing the kidney to strengthen the bone, with satisfactory therapeutic results reported as follows.     

银杏叶提取物活血通络作用的药效学研究

目的：观察银杏叶提取物(EGb)的活血通络功效。方法：小鼠耳廓微循环实验、胶原蛋白-肾上腺素诱导的小鼠体内血栓形成实验、大鼠血液流变学实验及离体兔耳灌流实验。结果：银杏叶提取物经口给药．能显著改善小鼠耳廓微循环，抑制胶原蛋白-肾上腺素诱导的小鼠体内血栓形成，降低大鼠血液粘度，并能显著扩张离体兔耳血管。结论：银杏叶提取物有显著的活血化瘀功效。     

自发免疫耐受大鼠移植肝内调节性T细胞的生物学特性

目的研究大鼠肝移植后自发免疫耐受的形成与移植肝内CD4~ CD25~ 调节性T细胞(Tr细胞)的关系。方法按供、受者不同将实验分为3组。急性排斥组:DA大鼠为供者,LEW大鼠为受者;自发耐受组:LEW大鼠为供者,DA大鼠为受者;同基因组:供、受者均为DA大鼠。各组均建立大鼠原位肝移植模型。分别在肝移植术后4、7、14、30和90 d时采用密度梯度离心法分离移植肝内淋巴细胞,免疫磁珠分离(MACS)法分选出CD4~ CD25~ Tr细胞;用流式细胞术(FCM)检测细胞纯度,同时分析CD4~ CD25~ Tr细胞比例的变化;体外细胞增殖试验研究CD4~ CD25~ Tr细胞对CD4~ CD25~-T细胞的免疫抑制作用。结果肝移植早期,急性排斥组和自发耐受组移植肝内CD4~ CD25~ Tr细胞比例均明显增加,其中急性排斥组增加更为明显;移植后4 d左右,两组CD4~ CD25~ Tr细胞比例开始下降,急性排斥组的下降幅度较大;移植后30 d,自发耐受组受者的移植肝内CD4~ CD25~ Tr细胞比例达到第2次高峰,约在移植后90 d时下降至正常生理水平。移植后7 d左右,急性排斥组受者均因发生排斥反应而死亡,而自发耐受组受者均存活。此外,CD4~ CD25~ Tr细胞能有效抑制CD4~ CD25~-T细胞的增殖。结论CD4~ CD25~ Tr细胞是一种具有特异免疫调节功能的T细胞亚群,其主动的免疫抑制功能可能是诱导大鼠肝移植自发免疫耐受的机制之一。     

交叉韧带重建手术中可吸收挤压螺钉的应用

目的探讨交叉韧带重建术中可吸收挤压螺钉的使用方法和疗效。方法总结53例交叉韧带重建病例使用可吸收挤压螺钉的情况，在术中、术后并发症、术后康复、膝关节功能状况等方面进行回顾性分析。结果三例出现韧带切割现象。两例股骨侧螺钉拧入后导引针无法拔出。一例挤压螺钉断裂。术后Lysholm评分平均92.4±4．1。结论交叉韧带重建术中使用可吸收挤压螺钉固定的方法固定牢固，术后恢复快，利于早期康复。     

家兔肝的神经支配

本实验共用家兔17只,将20%的HRP溶液100μl,经肝门多点注入,其中10只家兔出现标记细胞.两侧T_(2-12)背根节内共270个,左侧占51.11±3.04%,右侧为48.89±3.04%,左、右侧差异无显著性(P>0.05).在T_(5-8)背根节内的标记细胞较多,占总数的59.26±2.99%,中、小型细胞共占94.53±1.60%.两侧结状神经节内的标记细胞共143个.左侧占53.15±4.17%,右侧占46.85±4.17%.两侧差异无显著性.中、小型细胞共占93.01±2.13%.两侧腹腔神经节内的标记细胞较多,计数其中2例,共582个,左侧占59.79±2.03%,右侧为40.21±2.03%.在迷走神经背核内仅发现2个标记细胞.