妊高征合并胎儿宫内发育迟缓者胎盘病理改变与血管细胞粘附分子-1表达水平的关系

目的探讨妊高征(PIH)合并胎儿宫内发育迟缓(IUGR)时胎盘病理改变与胎盘组织中血管细胞粘附分子-1(VCAM-1)表达水平变化的关系。方法选取本院2001年1~9月分娩的妊高征合并IUGR患者(实验组)胎盘30例、单纯IUGR(IUGR组)28例、单纯PIH(PIH组)25例、正常妊娠(正常妊娠组)30例,常规HE染色及PAS染色,观察胎盘形态学变化。利用免疫组化方法对胎盘组织切片染色,观察子宫蜕膜层中螺旋动脉、绒毛组织滋养细胞及绒毛中毛细血管的VCAM-1表达。结果实验组中22例(73.33%)胎盘有明显病理改变,有病理改变的胎盘蜕膜组织内血管内皮细胞及胎盘绒毛毛细血管内皮VCAM-1的表达显著高于无病理改变者(P<0.001);而绒毛上皮组织滋养层细胞VCAM-1的表达则显著低于无病理改变者(P=0.019)。结论妊高征合并IUGR时胎盘发生了明显的病理改变,这种病理改变与胎盘中VCAM-1的异常表达密切相关。     

外周血细胞DNA、RNA及血红蛋白同时提取法

采用淋巴细胞分离液分离外周血中的有核细胞。经细胞裂解液处理，上清提取RNA，沉淀提取DNA，用分离液分离所得红细胞沉淀制备血红蛋白溶液，经对抽提物质量进行评价，发现3种提取物质量均高，本法能从少量外周血中同时分离DNA、RNA及血红蛋白，高效易操作，值得推广。     

细胞间粘附分子-1单抗防治初发期急性肾小管坏死的实验研究

目的探讨白细胞的粘附在初发期急性肾小管坏死(ATN)的病理生理学过程中所起的作用。方法Wistar雄性大鼠32只,随机分为4组。肌肉注射甘油制作ATN动物模型,应用细胞间粘附分子-1单抗防治初发期大鼠ATN,观察其肾脏病理学及血尿素氮和血肌酐变化。结果给药24 h后治疗组血肌酐值[(412.31±94.42)μmol/L]明显低于CD3对照组血肌酐[(990.21±171.25)μmol/L](P<0.05)。模型组与CD3对照组可见明显肾小管坏死,而治疗组仅2例出现较轻的坏死灶。结论白细胞的粘附在ATN发病过程中具有重要作用,应用细胞间粘附分子-1单抗阻断白细胞的粘附能明显减轻肾脏病理改变。     

水溶性氮酮在生发灵酊剂中对阿魏酸体外透皮吸收的促进作用研究

目的考察水溶性氮酮在生发灵酊剂中对阿魏酸透皮吸收的促进作用并找出最佳促透浓度。方法采用双室扩散小室,用高效液相色谱仪(HPLC)法分别测定含5种浓度水溶性氮酮的生发灵酊剂经离体鼠皮透皮吸收10h各时间点阿魏酸的释药量。结果当溶液中含水溶性氮酮为1.509%、吸收10h后累积释药量为2356.7μg,释放速率最快为242.9μg/cm2·h-1。结论水溶性氮酮在生发灵酊剂中对阿魏酸的透皮吸收具有促进作用,其最佳促透浓度为1.509%。     

培养大鼠星形胶质细胞牵张损伤后超微结构的变化

目的研究体外培养星形细胞牵张损伤后超微结构的变化。方法取新生1~2 d大鼠的皮层细胞原代培养,经纯化后传代培养于乳胶膜培养皿中。采用计算机控制的牵张损伤装置,分别以50、150、250 kPa压力牵张损伤培养于乳胶膜上的大鼠皮层星形胶质细胞。用3%戊二醛固定后,行扫描电镜和透射电镜观察。结果当用50 kPa驱动压力进行牵张损伤时,细胞结构即有明显破坏,表现为细胞间隙增宽,部分胞体和突起被撕裂;以50 kPa牵张损伤后1 h,透射电镜下可见线粒体肿胀、嵴减少;损伤后6 h可见线粒体致密、细胞器减少等。当牵张应力增大,星形细胞损伤程度加重,细胞器明显减少,线粒体空泡化,微丝、微管明显减少,直至水样胞质或致密胞体。结论较小的应力即可致星形细胞紧密连接的破坏和超微结构的改变,可能与脑外伤后产生广泛的脑水肿有关。     

头胸导联、常规导联心电图诊断冠心病的比较——与冠脉造影结果对照

同步记录72例拟诊冠心病患者头胸导联(HC)及常规导联(wilson)心电图(ECG),并与冠脉造影结果对照。结果:HC与Wilson导联ECG对冠心病诊断的敏感性分别为88.9％与81.5％,特异性33.3％与27.8％,准确性75％与68.1％;二组导联对比无明显差异(P>0.05);对右心室梗塞、缺血及左心室后壁缺血.HC导联的敏感性(91.7％)及准确性(94.4％)均显著高于Wilson导联(12.5％、70.8％,P<0.01),并与冠状动脉病变程度呈高度正相关。HC导联优于Wilson导联。     

脊柱推拿时咔哒声响的测量技术分析与设计

目的 为研究脊住推拿时所发出咔哒声响，提供一套可采集、定位和分析的检测仪器。方法 在回顾咔哒声响测量技术现状的基础上，分析研究了实现声响测量的技术路线，设计了由时钟脉冲发生器、发送／接收接口、校准波发生器、复合传感器、计算机、测定分析软件以及输出部件等组成的声响测量系统。结果 经初步试验，在40cm的距离上测到了关节发出的咔哒声响，说明技术上基本可行。结论 本仪器可以作为脊柱推拿所致咔哒声响的采集、定位和分析系统使用。     

营养干预对骨髓移植病人抗氧化力及脂质过氧化的影响

目的探讨外源性补充抗氧化性维生素对骨髓移植病人抗氧化力及脂质过氧化的影响。方法将19名骨髓移植病人随机分为实验和对照组,实验组在预处理前给予维生素C(300 mg/d)和维生素E(600 mg/d),对照组不予补充,其余处理两者相同。测定移植前后两组病人的血浆总抗氧化力、丙二醛、全血谷胱甘肽过氧化物酶及红细胞超氧化物歧化酶水平。结果(1)移植前后实验组血浆总抗氧化力、谷胱甘肽过氧化物酶和红细胞超氧化物歧化酶值一直处于正常或正常值附近,对照组则低于正常值,两组相比有显著差异;(2)移植后实验组丙二醛值很快降至正常,对照组则一直处于较高水平,两组有显著差异。结论移植前外源性补充抗氧化性维生素可提高骨髓移植病人机体的抗氧化力,减少了脂质过氧化物的产生,有效缓解移植前大剂量放、化疗对病人机体造成的高氧化胁迫压力。     

改良以聚羟基乙酸为支架同种异体组织工程化塑形软骨的构建

OBJECTIVE: To improve the method for constructing allogeneic molded cartilage by means of tissue engineering techniques. METHODS: The chondrocytes from the rib and articular cartilage of infant rabbits were harvested by type II collagenase digestion, followed by in vitro cell culture for 3 to 4 passages. The chondrocytes were then prepared into cell suspension and seeded onto C -and O -shaped pre-molded polyglycolic acid (PGA) scaffolds form chondrocyte-PGA composites, which were subsequently cultured in vitro for 7 to 10 d before implanted subcutaneously into adult rabbits. Improvement was made upon conventional shaping and implantation procedures. Morphological observation and cartilage regeneration assessment were conducted at different time points following the implantation, in comparison with the observation by conventional shaping and implantation methods. RESULTS: During in vitro cell culture, the rate of viable chondrocytes in the final cell suspension was (92+/-2)% after well-controlled prolongation of digestion trypsin, similar to the viable cell rate (93+/-2) % by traditional procedures (P>0.05). Gross observation found milk-white, newly generated cartilage which had good flexibility 4 weeks after implantation, and after 8 weeks and later, the cartilage took on the color of porcelain-white. Histological examination showed a few inflammatory cells around the newly generated immature cartilage 4 weeks after implantation, and the inflammation abated when the newly generated cartilage acquired similar histological properties to that of the original cartilage 8 weeks postoperatively and later. After 16 weeks, no blood vessel or capillaries were visible within the new cartilage. CONCLUSION: The chondrocyte viability is not affected when the cells are treated with well-controlled prolonged digestion with trypsin during in vitro cell culture. Improved PGA scaffolds shaping and the implantation procedure facilitate the regeneration of the cartilage after the implantation of the composites.     

Quinone oxidoreductase gene polymorphism and genetic susceptibility to nasopharyngeal carcinoma]

OBJECTIVE: To ascertain whether the point mutation of cDNA609 C to T of quinone oxidoreductase (NQO1) gene is associated with the genetic susceptibility to nasopharyngeal carcinoma. METHODS: Polymerase chain reaction-restriction fragment length polymorphism was employed to assess the polymorphism of NQO1 genes both in 120 patients with nasopharyngeal carcinoma and 120 healthy subjects matched for age, gender and origin. RESULTS: The allelic frequency of mutant T allele of NQO1 gene was significantly higher in patients with nasopharyngeal carcinoma than in the healthy subjects (P<0.05), and the individuals with T allelic genotype of NQO1 gene were at greater risk to develop nasopharyngeal carcinoma. CONCLUSION: The cDNA 609 T allele of NQO1 gene is very likely to be associated with the genetic susceptibility to nasopharyngeal carcinoma.