锡矿工人接尘与矽肺危险度评价

目的探讨粉尘暴露与矽肺危险度之间的接触效应关系。方法选择广西４个锡矿１９６０～１９６５年间工作１年以上的３０１０名接尘工人进行队列研究。用生存分析法统计累积粉尘接触量和矽肺出现的关系。结果追访到１９９４年底,检出矽肺１０１５例（３３．７％）。矽肺平均潜伏期２１．３年。总粉尘浓度７．５ｍｇ／ｍ３（ＴＷＡ）。拟合生存分析模型表明：矽肺发病危险度与累积接尘量的关系适合Ｗｅｉｂｕｌ分布。累积总粉尘接触量低于１０ｍｇ·ｍ－３·ａ－１时,矽肺危险小于１％;累积总粉尘接触量超过２０ｍｇ·ｍ－３·ａ－１时,矽肺累积危险度升高加快;累积总粉尘接触量达１５０ｍｇ·ｍ－３·ａ－１时,矽肺危险超过６８％。接尘时间与接尘量和矽肺危险度呈正相关。结论矽肺危险度与累积接尘量之间存在接触效应关系。     

氟暴露对工人血清IL-8及NO含量的影响

选取某铝厂工人６６ 人,观察血清氟、尿氟浓度,血清ＩＬ－ ８ 及ＮＯ含量变化情况。结果显示：和对照组比较,氟暴露组血清氟浓度和尿氟浓度明显增高;血清ＩＬ－ ８ 含量高工龄组升高,而低工龄组无变化;血清ＮＯ 含量低工龄组升高而高工龄组降低。提示机体染氟后通过各种正向或反向调节维持正常的免疫应答。其中ＩＬ－ ８ 及ＮＯ参与了复杂的免疫调节。     

灰关联聚类方法及其应用研究

本文以灰色系统理论中的关联分析方法为基础,建立了评价医院的聚类方法,并应用该方法对某市地区十一所医院的经营效益和管理水平进行了评价分析,获得较高的信度和效度。     

Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of RT-PCR-Am-plified CK19- and CEA-mRNA

ＲＴ－ＰＣＲａｍｐｌｉｆｉｃａｔｉｏｎｏｆｔｕｍｏｒ－ａｓｓｏ－ｃｉａｔｅｄ（ｃａｒｃｉｎｏｅｍｂｒｙｏｎｉｃａｎｔｉｇｅｎ）ａｎｄ（ｏｒ）ｏｒｇａｎ－ｓｐｅｃｉｆｉｃ（ｃｙｔｏｋｅｒａｔｉｎ１９）ｍＲＮＡｈａｓｂｅｅｎｕｓｅｄｍａｉｎｌｙｆｏｒｔｈｅｄｅｔｅｃｔｉｏｎｏｆｃｉｒｃｕ－ｌａｔｉｎｇｔｕｍｏｒｃｅｌｌｓａｎｄｍｉｃｒｏｍｅｔａｓｔａｓｅｓｉｎｓｏｌｉｄｔｕｍｏｒｓ［１］．Ｏｒｉｇｉｎａｌｌｙ,ｔｈｅｐｒｏｄｕｃｔｏｆａｍｐｌｉｆｉｃａｔｉｏｎｗａｓａｎａｌｙｚｅｄｂｙｇｅｌｅｌｅｃ…     

Clonal growth of human acute leukemia cells in serum-free methylcellulose medium

Ｔｈｅｇｒｏｗｔｈｏｆｌｅｕｋｅｍｉａｉｎｓｅｍｉｓｏｌｉｄｃｕｌｔｕｒｅｉｎｖｉｔｒｏｗａｓｄｅｐｅｎｄｅｎｔｏｎｔｈｅｐｒｅｓ－ｅｎｃｅｏｆｅｘｏｇｅｎｏｕｓｓｅｒｕｍ．Ｓｅｒｕｍｉｓａｍｉｘ－ｔｕｒｅｃｏｎｔａｉｎｉｎｇｌａｒｇｅｎｕｍｂｅｒｏｆｕｎｉｄｅｎｔｉｆｉｅｄｃｈｅｍｉｃａｌｃｏｍｐｏｎｅｎｔｓａｎｄｕｎｉｄｅｎｔｉｆｉｅｄｂｉｏ－ｌｏｇｉｃａｌｌｙａｃｔｉｖｅｓｕｂｓｔａｎｃｅｓ［１］,ｗｈｉｃｈｐｒｏｖｉｄｅｔｈｅｎｕｔｒｉｅｎｔｓｎｅｃｅｓｓａｒｙｆｏｒｔｈｅｃｅｌｌｇｒｏ…     

Effect of astragalus on the endothelin in rats with acute lung injury

Ｔｈｅｐａｔｈｏｌｏｇｉｃａｌｆｅａｔｕｒｅｓｏｆａｃｕｔｅｌｕｎｇｉｎｊｕｒｙｉｎｖｏｌｖｅｓｔｈｅｌｕｎｇｅｄｅｍａａｎｄｌｕｎｇｍｉ－ｃｒｏａｔｅｌｅｃｔａｓｉｓｉｎｄｕｃｅｄｂｙｔｈｅｄａｍａｇｅｏｆａｌｖｅｏｌａｒ－ｃａｐｉｌｌａｒｙｂａｒｒｉｅｒ．Ｃｌｉｎｉｃａｌｓｅｖｅｒｅａ－ｃｕｔｅｌｕｎｇｉｎｊｕｒｙｃｏｕｌｄｂｅｍａｎｉｆｅｓｔｅｄｂｙｒｅｓ－ｐｉｒａｔｏｒｙｄｉｓｔｒｅｓｓａｎｄｒｅｆｒａｃｔｏｒｙｈｙｐｏｘｉａ,ｏｒｓｏ－ｃａｌｌｅｄａｃｕｔｅｒｅｓｐｉｒａｔｏｒｙｄｉｓ…     

P21 WAF1/Cip1 Gene Expression in Primary Human Hepatocellular Carcinoma and its Relationship with P53 Gene Mutation

Ｐ２１ＷＡＦ１／Ｃｉｐ１ｐｒｏｔｅｉｎ（ｐ２１）ｉｓｔｈｅｐｒｏｄｕｃｔｏｆｗａｆ１ｇｅｎｅ,ａｌｓｏｋｎｏｗｎａｓｃｉｐ１,ｓｄｉ１［１,２］．Ｐ２１ＷＡＦ１／Ｃｉｐ１ｉｓａｕｎｉｖｅｒｓａｌｉｎｈｉｂｉｔｏｒｏｆｃｙｃｌｉｎ－ｄｅｐｅｎｄｅｎｔｋ...     

Experimental study on the antitumor effect of mouse B7-1 gene

ＴｗｏｓｉｇｎａｌｔｈｅｏｒｙｆｏｒＴｃｅｌａｃｔｉｖａｔｉｏｎｐｒｏｖｉｄｅｄａｎｅｗａｐｐｒｏａｃｈｆｏｒｔｕｍｏｒｇｅｎｅｔｈｅｒａｐｙ［１－２］．Ｅｘｐｒｅｓｓｉｏｎｏｆｃｏｓｔｉｍｕｌａｔｏｒｙｍｏｌｅｃｕｌｅｇｅｎｅｉｎｔｒａｎｓｆｅｃｔ...     

Effects of serum of Kawasaki disease on PDGF expression in monocytes and on endothelial cell apoptosis

ＴｈｅｃｏｒｏｎａｒｙａｒｔｅｒｙｃｏｍｐｌｉｃａｔｉｏｎｃｏｕｌｄｂｅｆｏｕｎｄｉｎａｌｍｏｓｔａｌｃａｓｅｓｏｆＫａｗａｓａｋｉｄｉｓｅａｓｅ［１］．Ｉｔｈａｓｒｅｃｅｎｔｌｙｂｅｅｎｓｈｏｗｎｔｈａｔｐｌａｔｅｌｅｔ－ｄｅｒｉｖｅｄｇｒｏｗｔｈ...     

The construction ofSchistosoma Japonicum vaccine BCG-Sj26GST and its identification

Summary The expression of foreign gene,Schistosoma Japonicum 26 ku antigen (Sj26GST),in Bacillus Calmette-Guerin (BCG),Mycobacterium CM. smegmatis) andEscherichia coli (E. coli) were studied. The cDNA fragment encoding Sj26GST was amplified by PCR using plasmid pGEX, which could express Sj26GST inE. coli as template. The Sj26GST cDNA was cloned into the downstream of humanM. tuberculosis heat shock protein (hsp) 70 promoter with correct reading frame, and then the DNA fragment containing hsp70 promoter and Sj26GST gene were subcloned together intoE. coli-Mycobacteria shuttle plasmid pBCG-2000 to construct the expression shuttle plasmid pBCG-Sj26. The recombinant BCG andM. smegmatis mc²155, which were electroplated with pBCG-Sj26, could express Sj26GST and the recombinantSchistosoma Japonicum vaccine BCG-Sj26GST was made. The recombinant Sj26GST (rSj26GST) were soluble and could be observed on SDS-PAGE at molecular weight of 26 ku. The content of rSj26GST accounted for 15% and 10% of total bacterial protein in BCG andM. smegmatis respectively. The results of Western blot showed the combination of rSj26GST with antibody of GST. This project was supported by Primary Foundation of China (No. 94-Y-19), Chinese National Nature Science Foundation (No. 339480022) and Chinese Health Foundation (No. 94-1-131).