Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction

Accumulating evidence points to a critical role of the brain gut axis as an important paradigm for many central nervous system diseases. Recent studies suggest that propolis has obvious neuroprotective properties and functionality in regulating intestinal bacteria flora, hinting at a potential key effect at both terminals of this axis regulation. However, currently no clear evidence confirms the effects of propolis on alcohol-induced depression. Here, we establish an alcoholic depression model with C57BL/6J mice and demonstrate that treatment with propolis protects against alcohol-induced depressive symptoms by behavioral tests. In addition, propolis attenuates the injury of nerve cells in the hippocampal region and restores the serum levels of brain-derived neurotrophic factor (BDNF) and dopamine (DA) in mice with alcohol-induced depression. Pathology and biotin tracer assays show that propolis repairs the intestinal leakage caused by alcohol. Additionally, propolis treatment increases the expression levels of intestinal intercellular tight junctions’ (TJs’) structural proteins Claudin-1, Occludin and zona occludens-1 (ZO-1), as well as the activation state of the liver kinase B1/AMP-activated protein kinase (LKB1/AMPK) signaling pathway, which is closely related to the intestinal permeability. Furthermore, propolis can reduce the levels of pro-inflammatory, lipopolysaccharide (LPS) and fatty-acid-binding protein 2 (FABP2), suggesting the significance of the inflammatory response in alcoholic depression. Collectively, our findings indicate that propolis exerted an improving effect on alcohol-induced depressive symptoms by ameliorating brain gut dysfunction.     

慢性阻塞性肺疾病危险因素的调查

目的探讨慢性阻塞性肺疾病（COPD）的危险因素及患病率。方法应用问卷调查的方法,对410例青岛市周边农村居民COPD危险因素及患病率进行调查。结果COPD总患病率为7.0%,其中男性为7.9%,女性为5.9%。COPD的危险因素包括吸烟、燃料种类以及遗传性。结论吸烟、燃料种类、遗传性与患COPD的危险性有关。     

PC法MRA颅内静脉窦成像分析

目的:评价相位对比法(PC)磁共振血管成像(MRA)对颅内静脉窦的显示能力和效果,以及不同参数对成像质量的影响。方法:对192例无明显颅内病变者行MRA检查,其中2DPC法165例,3DPC法15例。另选12例健康志愿者行MRA多参数检查进行比较。结果:PC法MRA用以显示静脉窦时,最佳流速编码(Venc)为10～20cm/s,在此基础上随Venc逐渐增大,静脉窦信号强度下降;相同Venc的3DPC法与2DPC法MRA静脉窦的信号强度和显示率无明显差异;2DPC法MRA在优势侧横窦可见到"模糊伪影"。结论:3DPC法是显示静脉窦的有效MRA方法;2DPC法简便易行,可作为静脉窦检查的起始方法。     

加速康复外科理念下针刺治疗腹腔镜下前列腺癌根治术后尿失禁的疗效观察

目的基于加速康复外科理念下观察针刺治疗腹腔镜下前列腺癌根治术后尿失禁的临床疗效。方法将2018年1月至2019年11月青岛市市立医院收治的48例腹腔镜下前列腺癌根治术后尿失禁患者,分为试验组和对照组(每组24例),试验组采用针刺联合盆底肌训练疗法,对照组采用盆底肌训练法。观察两组患者在治疗前后控尿的变化。结果两组组内治疗前后相比1 h尿垫试验漏尿量(g)、ICIQ-SF评分、24 h尿失禁次数均有改善;试验组治疗后各项观察指标均显著低于对照组,总有效率、治愈率均显著高于对照组;两组未见明显不良反应。结论针刺能够有效改善腹腔镜下前列腺癌根治术后患者尿失禁症状,促进尿控功能的恢复,符合加速外科康复理念,值得推广。     

消癌平制剂质量标准研究

目的 建立统一的消癌平制剂的质量标准.方法 采用高效液相色谱法,对消癌平制剂进行定性、定量测定.结果 通关藤对照药材高效液相图谱主峰清晰;绿原酸进样量在0.08～1.23 μg范围内与峰面积具有良好的线性关系,平均回收率为97.21%.结论 所建立的质量标准,控制消癌平制剂质量,且方法简便、快速、准确.     

圆锥角膜家系中ZEB1基因突变的致病性分析

目的 分析圆锥角膜男性患者的临床表型及致病基因突变,寻找致病基因突变位点,探讨表型与基因型之间的关系.方法 收集1例圆锥角膜男性患者及其父母的临床资料,完善其眼科检查,用Pentacam角膜地形图及生物力学分析提高疾病诊断.提取该家系中全部成员的外周血基因组DNA,选取先证者进行全外显子组测序,最终通过家系内共分离验证...     

Current status of antifertility vaccines using gonadotropin immunogens

Immunological methods of birth control are being pursued by several routes. Leading this field are vaccines which may inhibit the function of the hormone human chorionic gonadotropin (hCG). Vaccines using at least three different immunogens and a variety of formulations have been in clinical trials during 1986. In this article Vernon Stevens describes the background of the research in this field, outlines the clinical trials planned or underway to test early hCG vaccine formulations, and gives a personal view of the research still needed to achieve a safe and effective vaccine.     

The influence of subsequent dehydroepiandrosterone,diaminopropane, phenobarbital,butylated hydroxyanisole and butylated hydroxytoluene treatment on the development of preneoplastic and neoplastic lesions in the rat initiated with di-hydroxy-di-N-propyl nitrosamine

The comparative modifying potential of dehydroepiandrosterone (DHEA), diaminopropane (DAP), phenobarbital (PB), butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on the development of lesions initiated by dihydroxy-di-n-propyl nitrosamine (DHPN) in F344 rats were investigated. DHEA, BHA and BHT were all associated with significant reduction in numbers of glutathione-S-transferase P form (GST-P) positive foci in the liver whereas PB brought about their enhanced development. BHT and PB exerted promoting activity on the incidence of thyroid adenomas while DAP similarly increased lung adenoma formation. The results illustrate the advantages to be gained from two stage experiments using broad spectrum carcinogen initiation for comparative analysis of ‘modifiers’ of the neoplastic process and suggest that studies of enzyme alteration within putative preneoplastic lesions may be directly relevant to elucidation of mechanisms underlying such modification.     

优化扫描参数宝石能谱CT测量腰椎骨密度临床效果评价

目的 探讨优化扫描参数的宝石能谱CT测量人体L2、L3椎体骨密度的临床应用效果.方法 研究组:本院同时接受腹部或腰椎宝石能谱CT检查和QCT检查的患者126例,男性60例,女性66例;年龄15～92岁.能谱CT采用优化后的扫描参数(管电流260 mA,球管转速0.8 s/r),重建的1.25 mm图像传至AW4.6工作...     

顺爽滋养去屑型洗发露CHL细胞体外染色体畸变试验

目的:检测顺爽滋养去屑型洗发露对CHL细胞染色体致畸变作用。方法:用体外方法检测顺爽洗发露在0.5 mg/ml、0.25 mg/ml、0.125 mg/ml时,代谢活化组(含S9mix)及非活化组(不含S9mix)CHL细胞的染色体畸变率。结果:顺爽洗发露代谢活化组(含S9mix)及非活化组(不含S9mix)的染色体畸变率均低于5%。结论:在本实验条件下顺爽滋养去屑型洗发露未诱发CHL细胞染色体畸变率增加。