<Emphasis Type="Italic">Mycoplasma hyorhinis</Emphasis>infection in gastric carcinoma and its effects on the malignant phenotypes of gastric cancer cells

Background  

Mycoplasma hyorhinis infection has been postulated to play a role in the development of several types of cancer, but the direct evidence and mechanism remained to be determined.     

Comparative evaluation of various solid phases for the development of coated tube assays for the estimation of progesterone in human serum,bovine serum and bovine milk

Immobilization of progesterone antibody using three polystyrene surfaces and two progesterone radiotracers for use in the development of a coated tube assay for the evaluation of progesterone levels in human serum, bovine serum and bovine milk was studied. The selection of the solid phase and the tracers were based on the maximum binding, non-specific binding, sensitivity and percentage recovery. Amongst the polystyrene tubes studied, streptavidin coated tubes showed the acceptable assay features such as low non-specific binding (0.5–1.0%), adequate sensitivity (0.13–0.16 ng/ml) and recovery (85–115%) for all the three sample matrices, human serum, bovine serum and bovine milk.     

A new dimeric diarylheptanoid from the rhizomes of Alpinia officinarum简

AIM： To study the chemical constituents of the rhizomes ofAlpinia officinarum Hance. METHOD： Compounds were isolated by repeated column chromatography, and their structures were elucidated on the basis of spectral analysis. The cytotoxic activities of these compounds were evaluated with the T98G and B 16F10 cell lines by the MTT assay. RESULTS： A dimeric diarylheptanoid, named alpinin B （1）, along with three known diarylheptanoids were obtained, and their structures were identified as alpinin B （1）, 1, 7-diphenyl-3,5-heptanedione （2）, （4E）-1, 7-diphenylhept-4-en-3-one （3） and （4E）-7- （4-hydroxyphenyl）- 1-phenylhept-4-en-3-one （4）. CONCLUSION： Compound 1 is a new dimeric diarylheptanoid. The biosynthetic pathway of 1 was speculated to originate from a Michael reaction between compounds 2 and 3. Compound 3 showed cytotoxicity against the human glioblastoma T98G cell line with IC50 of 27 μmol·L-1.     

X线照射后肺癌细胞肿瘤坏死因子mRNA的表达

目的探讨X线诱导和调节肺癌细胞株NCI-H596和A549肿瘤坏死因子(TNF-α)mRNA的表达作用及其临床意义。方法应用实时荧光定量RT-PCR技术,定量检测NCI-H596和A549细胞株照射前和接受不同剂量(2,5,10,20,30和40Gy)的X线照射后,以及受照30Gy后不同时间TNF-αmRNA的表达。同时进行集落形成试验,检测NCI-H596和A549细胞株的放射敏感性。结果集落形成试验结果显示,未照射的NCI-H596细胞株集落形成率为0.12～0.24;A549细胞株集落形成率为0.37～0.45。照射后,A549细胞的存活分数(SF)在2Gy时为47.3%±9.0%,4Gy时为18.0%±3.0%,6Gy时为6.0%±2.0%,8Gy时为1.4%±0.3%;NCI-H596细胞的SF在2Gy时为55.2%±51.0%,4Gy时为15.9%±9.2%,6Gy时为3.5%±1.7%;8Gy时为0.9%±0.6%;二者SF差异无统计学意义,二者的D0值和Dq值差异亦无统计学意义(P>0.05)。实时荧光定量RT-PCR检测显示,NCI2H596细胞受照后TNF-αmRNA表达逐渐升高,照射剂量达40Gy时,TNF-αmRNA表达水平达高峰,为对照组的83倍;受照后,1hTNF-αmRNA表达逐渐升高,6h达高峰,为对照组的568倍。A549细胞受照后,1hTNF-αmRNA表达即达高峰,为对照组的136倍。结论X线可诱导肺癌细胞株A549和NCI2H596产生TNF-α,且呈剂量、时间依赖性,可提高肺癌放射治疗的疗效,也可对正常组织和     

VEGF及其受体KDR在非小细胞肺癌中的表达及与肿瘤血管形成关系

目的：探讨血管内皮生长因子（VEGF）及其受体（KDR）的表达与非小细胞肺癌（NSCLC）血管形成的关系。方法：分别应用免疫组织化学S-P法和原位分子杂交法检测62例NSCLC组织和16例肺的良性瘤样病变组织中的VEGF和KDR mRNA的表达，并对血管进行染色、计数。结果：62例肺癌组织中46例有VEGF的表达（占74．29％），阳性表达位于肿瘤细胞胞膜及胞质；KDR mRNA在49例中有阳性表达（占79．03％），阳性表达位于肿瘤血管内皮细胞、肿瘤细胞胞膜及胞质。VEGF和KDR mRNA的表达与有无淋巴结转移和临床病理分期密切相关；VEGF和KDR mRNA阳性表达组微血管密度明显高于阴性表达组，P〈0．01。结论：VEGF和KDR的表达与NSCLC的发生、发展和转移可能密切相关，可作为评估NSCLC患者预后的一项指标。     

多脏器转移性肺癌肉瘤1例诊疗报告并文献复习

目的 通过复习该病例的诊治经过，提高对肺癌肉瘤等少见恶性肿瘤的诊断水平和探讨治疗方法。方法 该病例以右小脑占位为初现病灶，右肺小结节经病理诊断为肺癌肉瘤，治疗过程中相继发生纵隔淋巴结转移、肝转移、腹腔转移，先后予以手术、放疗和化疗。结果 肺原发灶手术切除并获取病理结果，小脑和腹腔转移灶手术完全切除，纵隔转移灶经放疗后稳定，肝多发转移灶经泰素＋卡铂方案化疗4周期后接近完全缓解。结论 晚期肺癌肉瘤经过综合治疗可获得一定的近期疗效，紫杉醇类可试用于肉瘤类恶性肿瘤的化疗。     

3-乙胺基丙酸乙酯的合成工艺改进

3-乙胺基丙酸乙酯是合成喹诺酮类抗菌药吡哌酸、依诺沙星的重要中间体,比较了分别以无水乙醇、氯仿、甲苯和环己烷为溶媒,用丙烯酸乙酯和无水乙胺在-5～0C制备目标物的结果,确定用环己烷做溶媒效果最好,收率85%,纯度98%.     

重组磷脂酶C的研究

磷脂酶C是一类磷脂酰键的水解酶,在生物生命活动中起着第二信使的重要作用,能够抑制血小板的聚集作用,存在于原核生物和真核生物中,尤其是细菌中产生的磷脂酶C可以模拟真核生物中的作用而受到关注。该文叙述了磷脂酶C的克隆和表达、重构的工程菌株及重组磷脂酶C的纯化制备。同时,还简介了作者的研究,重组磷脂酶C可能进一步研究制成Ⅰ类抗血小板聚集作用新药。     

辛伐他汀对高胆固醇血症患者的干预研究

目的观察高胆固醇血症患者血清可溶性CD40配体(sCD40L)及高敏C-反应蛋白(hs-CRP)水平,探讨辛伐他汀治疗3周后血清炎性标志物的变化。方法酶联免疫吸附试验法检测50例高胆固醇血症患者及40例健康对照组血清sCD40L水平,乳胶增强比浊法检测hs-CRP水平。结果高胆固醇血症患者血清sCD40L、hs-CRP显著高于对照组(P<0.01),且sCD40L与LDL-C呈显著正相关(r=0.53,P<0.01),hs-CRP与TC(r=0.37,P<0.01)及LDL-C呈显著正相关(r=0.42,P<0.01),辛伐他汀可有效降低sCD40L、hs-CRP水平。结论高胆固醇血症可能与炎性反应有关,通过升高炎性因子促进动脉粥样硬化形成,而辛伐他汀通过降低炎性因子对心血管系统起保护作用。