CD4^＋CD25^high Regulatory Cells in Peripheral Blood of NSCLC Patients

The proportion and changes of CD4 CD25high regulatory T cells (Trs) in peripheral blood of non-small cell lung cancer (NSCLC) patients were analyzed and their clinical significance explored. The peripheral blood was collected from 61 patients with NSCLC and 15 healthy controls. By using monoclonal antibodies, the blood samples were evaluated with the flow cytometry for lymphocyte subsets (CD3 , CD4 and CD8 ) and CD4 CD25high Tr cells. The results showed that the proportion of CD4 CD25high Tr cells in NSCLC group was significantly higher than in control group [(4.36±2.07) % vs (2.04±1.03) %, P<0.01]. The proportion of CD4 CD25 high Tr cells in late stage was higher than that in early stage [stages I II (2.26 0.6) %; stage III (3.28 1.38) %; stage IV (6.06±4.08) %] (P<0.05). Kaplan-Meier survival analysis revealed that the prognosis of the patients who had higher proportion of CD4 CD25high Tr cells in peripheral blood was worse (P=0.0026). In conclusion, the relative increase in CD4 CD25high Tr cells in peripheral blood may be related to im-munosuppression and tumor progression in patients with NSCLC. This finding suggests that CD4 CD25 high Tr cells in peripheral blood of NSCLC may be positive for prognosis analysis. The use of depletion of the CD4 CD25high Tr cell therapy to treat NSCLC patients may be an effective strategy.     

VLDL-R中配体结合重复序列的结合特性及结构分析

目的 研究VLDL-R中8个配体结合重复序列(1igand-bindingrepeats，LBR)在配体结合中的作用并探讨结合位点的结构。方法采用基因缺失诱变方法，构建不同LBR缺失的VLDL-R重组体。将其分别导人无LDL-R功能性表达的1dl-A7细胞。配体结合实验观察转染细胞与荧光标记的VLDL、β-VLDL的结合能力，并采用同源建模的方法预测受体N-端328个氨基酸的配体结合域的空间结构。结果 配体结合实验显示LBR1和LBR2对受体结合VLDL、pVLDL最为重要；LBR3对结合VLDL也有重要作用，但对结合pVLDL无明显影响。模型显示受体N-端328个氨基酸的配体结合域呈现弧形口袋样结构，前3个LBR结构紧凑，呈棒状，负电荷相对集中，与功能特性吻合，LBR5与LBR6之间的连接区赋予口袋结构一定的伸缩性，适应配体大小的变化。结论 受体N-端的前3个LBR含有与配体结合的位点。该区域特定的空间结构与理化特性是结合功能的重要基础。     

蛋白酶活化受体-1在鼻咽癌中的表达及意义

目的检测PAR1在鼻咽癌中的表达,探讨PAR1在鼻咽癌中表达的意义。方法免疫组织化学（SP法）检测28例良性鼻咽组织、61例鼻咽癌组织中PAR1中的表达;采用RT-PCR、Western Blot检测鼻咽癌细胞系CNE-1、CNE-2中PAR1表达并比较其表达差异。结果PAR1在鼻咽癌组织及两种鼻咽癌细胞株中均表达。PAR1在28例良性鼻咽组织表达为10例,61例鼻咽癌组织中表达48例。PAR1在鼻咽癌组织中的表达率明显高于在良性鼻咽组织中的表达率（P〈0．01）,高度恶性细胞株中PAR1的表达明显高于其低度恶性细胞株。结论PAR1的表达与鼻咽癌的发生、生长方式、分期、恶性程度等有关。PAR1在鼻咽癌中的表达可能介导鼻咽癌侵袭转移等恶性生物学行为。     

长效干扰素α治疗176例慢性病毒性肝炎的不良反应分析

目的:了解长效干扰素在慢性病毒性肝炎抗病毒治疗过程中出现的常见不良反应及罕见不良反应。方法:对近3年我院慢性病毒性肝炎患者在使用长效干扰素抗病毒治疗的过程中出现的药物不良反应进行回顾性调查分析。结果:176例患者共出现不良反应620例次,其中发热(56.8%)、肌肉酸痛(36.4%)、白细胞减少(81.8%)及流感样症状(44.3%)为长效干扰素α的常见不良反应;罕见的不良反应包括甲状腺功能异常、抑郁或精神异常、心脏毒性及间质性肺炎等。结论:在抗病毒及免疫调节治疗的过程中干扰素的不良反应发生率高,用药前有必要对患者进行相关的用药教育,督促患者定期返院进行各种相关检查,以便及时监测并发现严重的药物不良反应,根据病情调整剂量或停用。临床医师及药师应熟知干扰素可能引起的各种不良反应。     

A new dimeric diarylheptanoid from the rhizomes of Alpinia officinarum简

AIM： To study the chemical constituents of the rhizomes ofAlpinia officinarum Hance. METHOD： Compounds were isolated by repeated column chromatography, and their structures were elucidated on the basis of spectral analysis. The cytotoxic activities of these compounds were evaluated with the T98G and B 16F10 cell lines by the MTT assay. RESULTS： A dimeric diarylheptanoid, named alpinin B （1）, along with three known diarylheptanoids were obtained, and their structures were identified as alpinin B （1）, 1, 7-diphenyl-3,5-heptanedione （2）, （4E）-1, 7-diphenylhept-4-en-3-one （3） and （4E）-7- （4-hydroxyphenyl）- 1-phenylhept-4-en-3-one （4）. CONCLUSION： Compound 1 is a new dimeric diarylheptanoid. The biosynthetic pathway of 1 was speculated to originate from a Michael reaction between compounds 2 and 3. Compound 3 showed cytotoxicity against the human glioblastoma T98G cell line with IC50 of 27 μmol·L-1.     

茯苓细胞色素P450基因PcCYP的克隆与序列分析

目的 筛选与茯苓Poriacocos生长发育相关的细胞色素P450基因序列,进一步丰富对茯苓生长发育理解。方法通过分子克隆到1个茯苓细胞色素P450基因Pc CYP,并利用在线工具分析其序列,预测蛋白的理化性质、结构域等分子特征;利用MEGA5.0分别对氨基酸进行多序列比对和进化关系分析,并采用q RT-PCR方法检测不同发酵培养时间6、8、10d的相对表达量。结果 从茯苓中克隆到的Pc CYP存在可变性剪切,2个选择性剪切体PcCYP1和Pc CYP2CDS区长度分别为1590、1542bp,分别编码529、513个氨基酸。氨基酸序列分析发现其均具有保守的P450结构域,且具有信号肽和多个磷酸化修饰位点,属于分泌型的不稳定亲水蛋白。氨基酸序列多重比对及系统发育树结果显示PcCYP与担子菌Coprinopsis cinerea的CYP502距离最近,推测PcCYP和CYP502具有相似功能,即参与调控茯苓子实体的发育。定量PCR结果显示,选择性剪接变体Pc CYP1的表达量较Pc CYP2低,因此PcCYP2是Pc CYP基因的主要剪接体。结论 Pc CYP与茯苓子实体的生长发育密切相...     

中西医结合治疗胎儿宫内发育迟缓

目的 探讨胎儿宫内发育迟缓(IUGR)的治疗方法。方法 分别采用硫酸镁加中药治疗胎儿宫内发育迟缓67例,硫酸镁治疗61例,利用彩色超声多普勒对其临床疗效进行观察。结果 硫酸镁加中药的有效率高于硫酸镁,其差异具有显著性意义(P<0.05)。结论 硫酸镁加中药是治疗IUGR的有效方法之一。     

基于实证的团体干预对社区养老院老年人满意度的影响

目的 了解社区养老院老年人满意度现况,并探讨针对性团体干预对社区养老院老年人满意度的影响。方法 将58名社区养老院老年人按照楼层分为观察组和对照组,采用汉化版养老院入住老年人满意度问卷进行调查,对照组给予院内常规服务,观察组在此基础上根据满意度调查结果实施每周3次的团体活动。比较干预前后两组老年人满意度评分。结果 社区养老院老年人满意度评分为(127.02±18.66)分;干预后观察组活动、照顾者、幸福感、餐饮维度及满意度总分显著高于对照组(均P<0.05)。结论 养老院老年人满意度处于中等水平,基于调查结果实施团体干预能有效提升老年人满意度。     

Simultaneous effect of vacuum and ultrasound assisted enzymatic extraction on the recovery of plant protein and bioactive compounds from sesame bran

The latest studies on the extraction of bio-components from plant materials have elaborated on novel approaches to increase yield. This study evaluated the effect of vacuum operation parameters on the recovery of protein and antioxidant compounds from sesame bran. In this context, vacuum-ultrasound assisted extraction (VU) and vacuum-ultrasound assisted enzymatic extraction (VUE) methods were carried out, and the vacuum parameters were investigated using central composite design by response surface methodology (RSM). The independent parameters were found effective on protein yield, total phenolic content and antioxidant capacity values. Optimum conditions for VU were 21 min vacuum time, 72 min restoration time and 539 mmHg vacuum pressure; and for VUE were 1.82 AU/100 g enzyme concentration, 8 min vacuum time, 68 min restoration time and 238 mmHg vacuum pressure. The protein yields increased by 31.0 and 41.6 % with VU and VUE, respectively compared to standard alkaline extraction. SEM images revealed deteriorative effects of applied extraction methods. SDS-PAGE analysis was conducted to examine detailed protein fractionation and alcalase assisted extraction revealed small protein sub-units (<18 kDa) which implies the presence of potential bioactive peptides. Additionally, LC/Q-TOF/MS analysis was performed and organic compounds in sesame bran extracts were identified.     

康泰仙对小鼠肝脑组织SOD、GSH—Px、CAT、MDA影响的实验研究

目的通过测定小鼠肝脑组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、过氧化氢酶（CAT）的活性和丙二醛（MDA）含量来研究康泰仙（KOTAIS）对小鼠抗衰老的作用。方法采用D-半乳糖制作的衰老模型,将小鼠随机分为正常组、模型组、药物低、中、高剂量组。连续给药灌胃60d后,检测肝脑组织中的SOD、GSH-Px、CAT、MDA指标。结果各剂量组能提高小鼠肝组织中SOD、GSH—Px、CAT的活性,并能降MDA的含量,以低、中剂量组尤其明显。结论康泰仙（KOTAIS）具有较好的消除自由基、抗衰老的作用。