RBP4与FOXO1基因单核苷酸多态性与2型糖尿病的相关性研究

Objective To investigate the distribution of single nucleotide polymorphisms(SNPs) on retinol binding protein 4(RBP4) genes and forkhead box O1 (FOXO1) gene, and their relationships with the occurrence of type Ⅱ diabetes mellitus (T2DM) in Chinese Han population. Methods Totally ten SNPs on RBP4 and FOXO1 were determined in 384 T2DM patients and 384 normal controls by TaqMan probe genotyping and agarose gel electrophoresis methods. And their serum level of fasting blood glucose (FBG), total cholesterol (TC) and trigly- ceride (TG) were also estimated. Results For RBP4, there was no significance for various genetypes and alleles including - 803 G > A, + 5169 C > T, and + 6969 G > C between two groups (P > 0.05). Each genotype had no relationships with T2DM (using adjusted logistic regression models). No haplotype was associated with T2DM. For FOXO1, among seven SNPs typed, significant variation was found in the frequency distribution of rs7324943 G/T in the two groups(χ2=4.02, P = 0.044), and further stratification analysis showed that in subjects of aged 40 and non-hypertension, there was a higher risk of T2DM in GT heterozygous carriers than in GG homozygous carriers (OR = 1.47, 1.80), T allele carriers showed higher risk than non-T carriers (OR = 1.42,1.79). For rs17592236 C/T, though no significant frequency variation was found between two groups (χ2 = 0.39, P = 0.401), but in subjects of aged ≤ 40, stratification analysis showed dramatically increased risk of T2DM in CT and TT carriers than in CC carriers (OR = 6.33,10.15), T allele carriers showed 7. 11-fold higher risk than non-T carriers. A haplotype CT related to T2DM susceptibility was also found, which could decrease the risk of its carriers by 28%. Conclusions For BBP4, the polymorphisms of - 803 G > A, + 5169 C > T, and + 6969 G > C had no relationships with T2DM in Chinese Han population. For FOXO1, the polymorphism of rs7324943 G/T,rs17592236 C/T and a haplotype CT were found related to the susceptibility of T2DM in Chinese Han population. Yet further studies are necessary to explain the impact of these polymorphisms on the disease occurrence.     

复发性生殖器疱疹患者血清可溶性PD-1的表达和免疫学意义

Objective To determine the concentration of soluble PD-1 (sPD-1) in the sera of patients with recurrent genital herpes (RCH), and to explore the significance of abnormal expression of sPD-1 in RCH. Methods Serum samples were obtained from 88 healthy blood donors, 74 patients with RCH including 34 cases of outbreak-stage RCH and 40 cases of stable-stage RCH. The serum level of sPD-1 was measured by monoclonal antibody labeling and enzyme linked immunosorbent assay (ELISA). Results A significant difference was observed in the serum level of sPD-1 between the patients with RGH and the blood donors (33.06 ± 17.5 μg/L vs. 53.07 ± 26.3μg/L, P < 0.01) and between the patients with outbreak-stage RGH and those with stable-stage RGH (27.47 ± 12.9 μg/L vs. 37.71 ± 19.6 μg/L, P< 0.01). Conclusions There is a low expression of sPD-1 in patients with RGH, which may be one of the mechanisms underlying the escape of HSV- Ⅱ from immunologic surveillance and development of immunological tolerance.     

吡咯喹啉醌对γ辐射小鼠防护作用的初步研究

目的 研究吡咯喹啉醌(PQQ)对γ辐射小鼠的防护作用。方法 将40只昆明小鼠随机分四组,即未照射组、单纯照射组、照射前给药组、照射后给药组。给药组小鼠口服PQQ剂量按体重计每天2mg/kg,连续给药7天。采用⁶⁰Coγ射线单次全身照射,剂量5Gy。于照射后第8天,颈椎脱臼处死小鼠,收集血清、制备肝匀浆,进行各项生化指标测定。制作肝HE染色切片。结果 照射后小鼠血清及肝脏中SOD、T-AOC显著下降(P<0.05),MDA显著升高(P<0.05)。照射给药组小鼠血清SOD、T-AOC含量显著升高(P<0.05),MDA含量显著减低(P<0.05);肝脏中SOD含量显著升高(P<0.05)。所有照射组小鼠肝组织都有肝板排列紊乱,出现肝细胞水肿,变性、坏死现象。结论 γ辐射引发小鼠全身性的氧化应激,肝脏是重要的辐射敏感器官。PQQ对γ辐射小鼠的防护作用机制是:PQQ能直接清除自由基,同时能调动照射小鼠的全身自由基清除系统,尤其是SOD的活性,降低自由基含量。     

中西医结合治疗肝内胆汁淤积症对妊娠结局的影响

目的：观察中药结合熊去氧胆酸、能量合剂联合用药治疗妊娠期肝内胆汁淤积症及改善围产儿预后的疗效。方法：将100例妊娠期肝内胆汁淤积症患者随机分为治疗组和对照组,治疗组予中药方和熊去氧胆酸、能量合剂,对照组予熊去氧胆酸、能量合剂,疗程均为2周。跟踪治疗前后皮肤瘙痒缓解及总胆汁酸、肝转氨酶水平及围生儿结局。结果：治疗组总胆汁酸、肝转氨酶水平均有所下降,胎儿窘迫率、羊水污染率、新生儿窒息、早产率均较对照组降低,差异有统计学意义（P〈0.05）。结论：中药联合熊去氧胆酸、能量合剂治疗妊娠期肝内胆汁淤积症有良好治疗效果。     

茶多酚对人脐静脉内皮细胞增殖和凋亡的实验研究

目的 观察茶多酚(TP)对体外培养人脐静脉内皮细胞(HUVEC)增殖和凋亡的影响.方法 采用倒置相差显微镜、RT-PCR法进行HUVEC的观察及鉴别.应用MTT法和流式细胞仪测定不同浓度TP对HUVEC增殖、细胞周期、增殖指数及凋亡率的影响.结果 目的 条带测序结果 与GenBank人CD31基因序列完全一致.结论 TP能抑制内皮细胞增殖并诱导其凋亡,是一种潜在的新生血管抑制剂.     

三氯乙烯的致癌性

三氯乙烯是一种广泛使用的工业溶剂和常见的环境污染物。动物实验发现三氯乙烯可引起小鼠肝癌和大鼠肾癌,流行病学研究也表明三氯乙烯暴露与包括肝癌和肾癌在内的多种癌症具有相关性。2012年,国际癌症研究机构根据三氯乙烯对肾癌的明确作用将其列为Ⅰ类致癌物,但三氯乙烯对肝癌的影响以及其致肝癌和肾癌的分子机理仍不清楚。本综述结合文献复习和本实验室工作,从流行病学研究、动物实验以及分子机制等方面探讨三氯乙烯致肾癌和肝癌的进展。     

中性粒细胞抑制因子的作用及用途

中性粒细胞抑制因子 (NIF )是整合素LAF 1和Mac 1的配体。作为 β2 整合素的拮抗剂 ,与ICAM 1、ICAM 2竞争性地和LAF 1及Mac 1结合 ,抑制中性粒细胞与内皮细胞的粘附和聚集 ,阻止白细胞的渗透和浸润。因此 ,NIF能用于治疗各种出现白细胞浸润和活化的临床疾病及组织损伤 ,包括休克、中风、急性和慢性同种异体移植排斥反应、血管炎、脓毒症、风湿性关节炎、炎性皮肤病、肠炎、伴随心肌梗塞的局部缺血再灌注损伤和成人呼吸窘迫综合征等。NIF作为一种新的抗炎药 ,具有广阔的应用前景。     

热疗对人肺癌细胞凋亡的影响

目的探讨热疗对人肺癌A549、H1299细胞生长与凋亡的影响。方法 MTT法检测热疗对人肺癌A549、H1299细胞生长的抑制作用;流式细胞术观察热疗对人肺癌A549、H1299细胞周期和凋亡的影响;RT-PCR检测凋亡相关基因p53及Casepase-3的表达。结果热疗对人肺癌A549、H1299两种细胞的生长均有抑制作用。热疗可以明显改变人肺癌A549细胞生长周期的分布,G0/G1期细胞明显减少;但对人肺癌H1299细胞生长周期的分布影响不大。热疗能使A549细胞p53蛋白的表达明显增强,但不能诱导H1299细胞p53蛋白的表达。热疗能同时诱导A549、H1299两种细胞Caspase-3蛋白表达的增强,并且以A549细胞的表达尤为明显。结论热疗对两种肺癌细胞的生长均有抑制作用,可以增加细胞凋亡。p53基因可能在热疗诱导肺癌细胞凋亡和周期分布改变的过程中起主导作用。     

维生素C在肿瘤细胞代谢与凋亡中的作用

维生素C（Vitamin C,Vc）是具有许多生物学功能的水溶性已糖衍生物,在多数生物环境中以抗坏血酸的形式存在。Vc在体内氧化成脱氢Vc,并与后者形成可逆的氧化还原系统。Vc是细胞内外液中广谱的抗氧化物,在人体组织细胞代谢中具有多种重要的生理功能。     

人脐血CD56+细胞毒性淋巴细胞的扩增研究

目的:从人脐血单个核细胞(CBMC)中高效扩增CD56+细胞毒性淋巴细胞.方法:以干细胞生长培养基(SCGM)为基础培养基,设计不同浓度抗CD3单抗、IL-2的培养条件,从CBMC中诱导扩增CD56+细胞毒性淋巴细胞,用四甲基氮唑蓝(MTI)法检测其对肿瘤细胞株K562和Raji的杀伤活性,并与抗CD3单抗激活的杀伤细胞(CD3AK)、细胞因子诱导的杀伤细胞(CIK)进行比较.结果:在以SCGM为基础培养基时,CBMC经500ng/ml抗CD3单抗、500U/ml IL-2作用获得大量增殖,在12天时扩增(14.7±4.0)倍,(56.4±2.8)％为CD56+细胞,其中CD3-CD56+NK细胞和CD3+CD56+T细胞分别占细胞总数的(24.9±5.3)％和(30.0±4.7)％:在效/靶比10:1时,对K562和Raii的杀伤率分别为80.5％和55.3％,均显著高于CD3AK和CIK细胞.结论:在抗CD3单抗和IL-2作用下可使用SCGM,获得以CD56+淋巴细胞为主的强细胞毒性免疫效应细胞,为进行肿瘤过继免疫治疗提供了一种简单的扩增脐血CD56+细胞毒性淋巴细胞的方法.