B超引导宫腔镜治疗子宫纵隔的临床分析

目的:探讨B超引导在宫腔镜治疗子宫纵隔中的作用。方法:对85例子宫纵隔患者在B超引导下行宫腔镜子宫纵隔切开术的效果进行回顾性分析。结果:手术均一次完成,无子宫穿孔、脏器损伤等并发症发生,复查B超宫底部残留纵隔均<1 cm,宫腔形态基本正常,术后有效随访79例,均妊娠活产。结论:B超引导下行宫腔镜子宫纵隔电切术,是一种安全性高,效价比高的治疗方法。     

胎儿消化系统畸形产前超声诊断的追踪观察

目的探讨胎儿消化系统畸形的产前超声诊断与追踪结果的符合率。方法对产前超声检查诊断为消化系统异常的69例胎儿进行系统追踪随访。结果 69例产前超声诊断消化系统异常的胎儿中自然产23例(33.3%),引产46例(66.7%),有手术与尸体解剖结果的19例(19/69,27.5%),其产前超声诊断与最后诊断符合率为94.7%(18/19)。产前超声诊断消化系统畸形有一定的假阳性率,本组假阳性病例15例,新生儿期无明显临床症状。结论产前超声诊断胎儿消化系统畸形符合率高,系统追踪随访有助于进一步提高产前超声诊断率,能更好的提供临床咨询。     

电子探针分析乳牙釉质和牙本质钙含量的研究

目的研究乳牙釉质和牙本质中钙含量及分布,探讨其与龋患状况之间的关系。方法运用电子探针显微分析仪测定40颗离体下颌乳中切牙纵剖面上不同深度的钙含量,并以标本所在个体的龋患状况分组,分别进行统计学分析。结果 40例下颌乳中切牙标本的总计钙含量均值为37.13±1.10（wt%）。其中,牙釉质钙含量均值为38.31±0.94（wt%）,牙本质钙含量均值为36.66±1.38（wt%）;牙釉质表层下50μm、100μm处钙含量高于250μm处钙含量;牙本质釉牙本质界下50μm、100μm处钙含量分别低于450μm、500μm、550μm、600μm处钙含量。正常乳牙钙含量在无龋组、龋病低危组、龋病高危组间未见统计学差异。正常乳牙钙含量与龋补牙数、龋补牙面、龋蚀指数之间均无相关关系。结论正常乳牙中钙元素分布呈非均质状态。牙釉质钙含量从表层向釉牙本质界方向呈逐渐下降趋势;牙本质钙含量从釉牙本质界向髓腔侧呈逐渐上升趋势。发育正常的乳牙钙含量不能作为反映个体龋患状况的单一指标。     

乌司他丁和血必净抑制早期内毒素血症大鼠炎症反应的对比研究

目的研究乌司他丁(ulinastatin,UTI)抑制早期内毒素(LPS)血症大鼠的炎症反应是否优于血必净(Xue-bijing,XBJ)。方法通过鼠尾静脉注射LPS复制LPS血症大鼠模型。54只SD大鼠随机分为LPS组、(LPS+UTI)组、(LPS+XBJ)组,每组18只,分别在用药后8 h、24 h、48 h时点随机快速处死6只大鼠,取心脏血分离血清,用双抗体夹心酶联免疫吸附法测定白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)。结果三组中各时间点TNF-α、IL-6、IL-1分别比较差异均有统计学意义(P<0.05);(LPS+UTI)组在8 h时间点与(LPS+XBJ)组比较差异有统计学意义(P<0.05),在24 h、48 h时间点差异无统计学意义(P>0.05)。结论 UTI和XBJ均能有效抑制LPS大鼠的炎症反应;UTI在炎症早期抑制炎症反应优于XBJ。     

妊娠期妇女外阴阴道假丝酵母菌携带情况与相关因素分析

目的了解深圳地区妊娠期妇女外阴阴道假丝酵母菌携带情况与其相关影响因素。方法对产前门诊的2200例孕妇进行阴道分泌物假丝酵母菌检测,了解假丝酵母菌阳性率,并对所有对象进行问卷调查,了解相关影响因素并进行统计分析。结果深圳地区妊娠期妇女外阴阴道假丝酵母菌携带率为18.05%,携带率随孕周增长而增加,妊娠中、晚期携带率较妊娠早期显著升高（P〈0.01）。检测阳性的孕妇肥胖、孕前VVC病史、血糖异常、生活习惯不良的比例显著高于检测阴性的孕妇（P〈0.01）。结论深圳地区妊娠期妇女外阴阴道假丝酵母菌携带率在正常范围内,对存在肥胖、孕前VVC病史、血糖异常、生活习惯不良等高危易感因素的妊娠妇女应进行阴道分泌物假丝酵母菌检测,以作为积极防治的依据。     

督脉电针联合人脐血MSCs移植对脑缺血大鼠VEGF蛋白表达的影响

目的:研究督脉电针联合人脐血MSCs移植对脑缺血大鼠VEGF表达的影响。方法:提取并培养人脐血MSCs,建立大鼠大脑中动脉闭塞模型,采用免疫组织化学染色检测不同时间段脑缺血边缘区VEGF的表达水平。结果:缺血后7 d和14 d,脐血MSCs移植组和联合治疗组VEGF阳性表达均高于PBS移植组(P<0.01),缺血后14d和28 d,联合治疗组高于人脐血MSCs移植组,差异具有统计学意义(P<0.05)。结论:督脉电针联合人脐血MSCs移植具有协同效应,可上调缺血区半暗带VEGF表达,促进血管新生,对改善缺血半暗带血供有重要意义。     

Induction of apoptosis of human ovarian cancer cells by SGI-1776 combination with DDP in sub-toxic concentration in vitro

Objective： The aim of the study was to investigate the effect of SG1-1776 combination with DDP in sub-toxic concentration on induction of apoptosis of human ovarian cancer HO-8910 ceiis in vitro and to unravei the associated mechanisms. Methods： Human ovarian cancer HO-8910 cells were cultured in vitro. The inhibitory effect of SG1-1776 combination with DDP in sub-toxic concentration on induction on viability of human ovarian cancer HO-8910 cells was evaiuated by the MTT assay. Cell apoptosis rate was analyzed by flow cytometry. The proteins expression level related to apoptosis were analyzed by Western blot. Results： SG1-1776 combination with DDP in sub-toxic concentration significantiy inhibited the proliferation of human ovarian cancer HO-8910 cells, and proliferation inhibition rate was increased drastically compared with normai saline （NS） group or DDP group in sub-toxic concentration or SG1-1776 group in sub-toxic concentration （P 〈 0.01）. Apoptosis rate markedly increased after the treatment of SG1-1776 combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of Bax and Cyto-c were depressed by SG1-1776 combination with DDP in sub-toxic concentration. Cenclusion： SG1-1776 combination with DDP in sub-toxic concentration could inhibit the cell proliferation and lead to cell apoptosis inhuman ovarian cancer HO-8910 cells, and its mechanism may be related to through mitochondrial apoptotic pathway.     

Prenatal diagnosis of diastematomyelia: a case report and review of the literature

Diastematomyelia is a rare congenital disorder in which the spinal cord is divided longitudinally for part of its length. It can be accurately identified in the prenatal period by detailed sonographic examination of the spine. We report a case of diastematomyelia localized to spinal levels T6-T9 diagnosed prenatally at 31 weeks' gestation and review the existing literature on the diagnosis and management of this condition during pregnancy.     

孕激素诱导阻断因子在反复自然流产患者血清中的表达

目的:通过测定反复自然流产(recurrent spontaneous abortion,RSA)患者血清中孕激素诱导阻断因子(progesterone-induced blocking factor,PIBF)的表达水平,探讨其与RSA的关系和临床意义。方法:选择RSA患者56例作为研究组,随机抽取同期的正常健康早孕妇女64例作为对照组,运用酶联免疫吸附法(ELISA)检测血清中PIBF水平,并进行对比分析。结果:RSA组PIBF水平明显低于对照组(P<0.05)。结论:PIBF与RSA的发生有关系,PIBF水平降低可能是引起RSA的发病原因之一。     

反复自然流产患者外周血中Th1/Th2型细胞因子的表达及其意义

目的:分析反复自然流产(RSA)患者血清中Th1/Th2型细胞因子水平的变化,探讨其在RSA发病中的意义.方法:采用酶联免疫吸附法检测56例RSA患者血清中Th1(IL-2、TNF-α、IFN-γ)/Th2(IL-10)型细胞因子水平,同时以正常健康早孕组64例作为对照.结果:RSA组血清中IL-2、TNF-α、IFN-γ的水平显著高于对照组(均P＜0.05),而IL-10水平显著低于对照组(P＜0.05).结论:RSA患者血清中Th1与Th2间平衡的改变可能是导致RSA的重要原因之一.