Green synthesis of a AgCl@AgI nanocomposite using Laminaria japonica extract and its application as a visible-light-driven photocatalyst

A AgCl@AgI composite photocatalyst was greenly synthesized using Laminaria japonica extract as the source of halogen anions, and characterized by XRD, SEM, TEM and XPS techniques. The photocatalytic activity and photochemical stability of the AgCl@AgI were investigated by the photodegradation of methyl orange (MO) azo dye under visible light illumination (λ > 420 nm). The AgCl@AgI composite showed good photochemical stability and much higher photocatalytic activity than that of single AgCl and AgI. Mechanism studies showed that the main active species are photoinduced holes (h⁺) and superoxide anion radicals (·O₂⁻). Finally, a plausible mechanism for the separation of photoinduced charge carriers was proposed.

A IgA@lCgA nanocomposite was greenly synthesized with Laminaria japonica extract and applied as a visible-light-driven photocatalyst for organic pollutant degradation.     

某高校女教职工乳腺红外线检查结果分析

目的了解某高校女教职工乳腺红外线检查结果及患病情况,为女教职工乳腺疾病的防治工作提供参考依据。方法收集整理健康体检中541名女教职工的乳腺红外线检查结果资料并做统计学分析。结果检出乳腺疾病211人．检出率39．00％。其中乳腺小叶增生检出率19．59％,乳腺轻度增生检出率16．08％．两者均为40-岁组检出率最高,分别为30．14％、21．92％,20～岁组次之,50～岁组检出率最低;乳腺囊性或结节性增生检出率2．40％;乳腺纤维瘤或肿块检出率0．92％。结论该高校女教职工乳腺不同程度增生检出率较高．应引起高度重视并积极治疗。定期进行健康体检和开展健康教育等保健工作很有必要。     

半夏与其伪品掌叶半夏的RAPD鉴别

目的建立半夏及其伪品掌叶半夏的快速简单的分子鉴定方法。方法 SDS法提取半夏和掌叶半夏的基因组DNA,20个10碱基随机引物(S1-S20,上海生工)用于RAPD-PCR分析。结果 20个引物中有7个有效稳定的引物在半夏和掌叶半夏之间能显示出多态性指纹图谱,其中S10和S17在半夏和掌叶半夏之间显示较大差异。结论利用引物S10和S17进行RAPD-PCR能有效鉴别半夏和掌叶半夏。     

淫羊藿研究新进展

淫羊藿目前的研究主要集中在化学成分,药理作用,药用植物新资源,质量评价,生态生理,分子鉴定以及资源保护和人工栽培等方面.其中药理研究尤为突出,包括淫羊藿对心脑血管、免疫系统、抗肿瘤、阻止骨疏松、抗衰老以及生殖系统等方面的作用.综述了淫羊藿各个方面的最新进展[1-5],并对淫羊藿的今后发展作了展望.     

Tumour cell kinetics as prognostic factor in surgically-treated-non-small cell lung cancer

The proliferative rate of tumour cells were studied in 80 non-small cell lung cancers (NSCLC) treated surgically at the Centre of Oncology in Kraków, between 1990 and 1996. There were 56 squamous cell carcinoma (SqLC) and 24 non-SqLC (18 adenocarcinoma (AcLC), three large cell carcinomas (LcLC), three mixed tumours). The proliferative potential of the tumour cells was studied on the basis of percentage of cells in the S-phase (S-phase fraction, SPF), proliferative index (PI, number of cells in S+ G₂/M phases), bromodeoxyuridine labelling index (BrdUrdLI), and predictive potential doubling time (pred T_pot). Significant differences in the proliferating rate between histological groups of tumours were shown by the BrdUrdLI. The 5-year survival time for patients with higher proliferating tumours (BrdUrdLI>4.1%, optimal cutoff level) was significantly higher (median survival time of >60 months) than those with lower proliferative potential (BrdUrdLI ≤4.1%) (median survival time of 19 months, P=0.0091). SqLC patients had significantly better 5-year survival (median survival time of 47.5 months) than those with non-SqLC (median survival time of 18.5 months). Cox multivariate analysis showed that only higher proliferation of the tumour cells (BrdUrdLI >4.1%), and lower clinical stage (I and II) were favourable prognostic factors in respect to patients' survival.     

四川黑熊线粒体细胞色素C氧化酶亚基Ⅰ基因的克隆及序列分析

目的探讨四川黑熊线粒体细胞色素C氧化酶亚基Ⅰ基因的特征。方法根据已经报道的细胞色素C氧化酶亚基Ⅰ（cytochrome c oxidase subunitⅠ,COXⅠ）基因设计引物、PCR扩增和直接测序,并用软件对四川黑熊及其他几个物种的COXⅠ基因序列及COXⅠ蛋白的氨基酸序列进行了分析。结果四川黑熊COXⅠ基因长1605bp,编码含514个氨基酸残基的前体蛋白。碱基的含量分别为A26.9%,C23.1%,G18.3%,T31.8%。起始密码子为“ATG”,终止密码子为“TAA”。蛋白质等电点为6.29,分子质量为57.2×10^3。以四川黑熊和已报道的其他6种动物的COXⅠ基因序列为数据构建的分子系统发育树表明在所比较的其他3种熊类中,四川黑熊和美洲黑熊的亲缘关系最近；在牛,狗和大鼠中,四川黑熊与狗的亲缘关系最近。结论四川黑熊COXⅠ基因的DNA序列和蛋白的氨基酸序列和其他动物的相应序列有很高相似性,表明COX基因在所比较的几个物种中具有高度保守性。     

加权打分法定量评价半夏种质资源的研究

目的:研究半夏优良种质评价新方法,为中药优良种质评价提供参考。方法:收集到7个省(市)的9个主产区栽培或野生种质,在田间进行完全随机种植实验,根据半夏特点选相应关键指标,通过打分法进行综合评价。结果:在收集到的种质中综合评价指数前2位的为四川安岳93.1、河北易县87.5,性状表现优良。结论:综合评价指数超过80的可作优良半夏种质,四川安岳和河北易县半夏可作优良种质推荐使用。     

Activity of pyrrolizidine alkaloids against biofilm formation and Trichomonas vaginalis

Crotalaria genus belongs to the subfamily Papilionoideae comprising about 600 species spread throughout tropical, neotropical and subtropical regions. In this study, seeds of Crolatalaria pallida were used to the isolation of usaramine, a pyrrolizidine alkaloid. Thus, Pseudomonas aeruginosa and Staphylococcus epidermidis were utilized as strains to test some activities of this alkaloid, such as antibiofilm and antibacterial. Meanwhile, monocrotaline obtained from Crotalaria retusa seeds, was used as the starting material for synthesis of necine base derivatives with anti-Trichomonas vaginalis potential. Alkaloids were characterized by 1D and 2D NMR techniques and GC–MS analysis. Usaramine demonstrated a highlighted antibiofilm activity against S. epidermidis by reducing more than 50% of biofilm formation without killing the bacteria, thus it could be assumed as a prototype for the development of new antibiofilm molecules for pharmaceutical and industrial purposes. Monocrotaline activity against T. vaginalis was evaluated and results indicated inhibition of 80% on parasite growth at 1 mg/mL, in addition, neither cytotoxicity against vaginal epithelial cells nor hemolytic activity were observed. On the other hand, retronecine showed no anti-T. vaginalis activity while azido-retronecine was more active than monocrotaline killing 85% of the parasites at 1 mg/mL. In conclusion, pyrrolizidine alkaloids are suggested as promising prototypes for new drugs especially for topical use.     

补阳还五汤配合丁咯地尔治疗脑梗塞32例临床观察

目的：观察补阳还五汤和丁咯地尔治疗脑梗塞的疗效。方法：将64例脑梗塞患者随机分成治疗组与对照组。每组32例,对照组进行西医常规治疗。治疗组加用补阳还五汤和丁咯地尔注射液,并在48小时后开始康复治疗。所有患者均于治疗前及治疗30天后进行疗效评分,运用简式Fugl-M efer运动功能评分法（Fugl-M efer Assess-m ent FMA）评定偏瘫侧的肢体运动功能;运用改良Barthel指数（Mod ified Barthel Index MB I）评定日常生活活动能力。结果：治疗组与对照组差异且有统计学意义（P〈0.01）。结论：补阳还五汤和丁咯地尔注射液能够有效的促进脑梗塞患者恢复健康,对其提高生活质量优于西药常规治疗。