硫氧还蛋白还原酶作为新型生物标志物在乳腺癌诊断和治疗中的价值

已有研究证明硫氧还蛋白还原酶在多种人原发肿瘤中过度表达,其活性在肿瘤人群和健康人群中有明显差异。本义研究了健康人群和乳腺癌患者中TrxR活性的不同,并探讨了硫氧还蛋白还原酶作为乳腺癌治疗中的一个新型治疗靶点的可能性。通过研究．我们第一次发现未经治疗的肿瘤患者的TrxR活性值水平明显高于健康人群。存乳腺癌患者中,TrxR活性值在不同的年龄组和癌症分期组没有明显的差异。当TrxR活性值小于10U／mL时,TrxR活性值和肿瘤影像学评价结果有很高的一致性。这些发现证明硫氧还蛋白还原酶可以作为一个有效的新型生物标志物用于对乳腺痛临床治疗效果的监测和评价,并能成为乳腺癌早期检测的一种方法。     

IVF versus ICSI for the fertilization of in-vitro matured human oocytes

Traditional dogma suggests that intracytoplasmic sperm injection (ICSI) should be performed to ensure successful oocyte fertilization in an in-vitro maturation (IVM) cycle. This study postulated that there would be no difference in the fertilization rate when ICSI was compared with IVF. This hypothesis was tested in a randomized trial of IVF versus ICSI in IVM. A total of 150 immature oocytes were collected in eight cycles of IVM for patients diagnosed with polycystic ovarian syndrome (PCOS). Patients were primed with minimal FSH before transvaginal oocyte aspiration. Sibling oocytes were inseminated by 50% IVF and 50% ICSI. There was no significant difference in fertilization, useable or total blastocyst development between the two insemination technique groups. Clinical pregnancy results for combined fresh and cryopreserved transfers were identical between the two insemination techniques with a total of two fresh and five cryopreserved IVF-inseminated embryos resulting in three clinical pregnancies (42.9%) and five fresh and two cryopreserved ICSI-derived embryos resulting in three clinical pregnancies (42.9%). This research has shown IVF to be a legitimate fertilization technique for IVM oocytes in PCOS patients and provides a greater awareness of the use of a fertilization method previously not utilized with IVM.In-vitro maturation (IVM) is an alternative treatment method to traditional IVF. Due to the minimal use of stimulating hormones in this treatment, IVM has a lower risk of ovarian hyperstimulation syndrome, it can be used for fertility preservation in cancer patients and it is more cost conservative. Early research into the effects of IVM showed a hardening effect on the membrane surrounding the egg (the zona pellucida). It was initially believed that, to overcome this hardening in order to allow the egg to be fertilized, spermatozoa would need to be injected into the egg using intracytoplasmic sperm injection. Due to recent advances in hormonal stimulation protocols (FSH priming) and culture conditions, we postulated that, for patients suffering from polycystic ovarian syndrome (PCOS), fertilization, embryo development and clinical pregnancy would not be superior in the injected oocytes compared with those inseminated by IVF. We found that by using the two insemination techniques on sibling oocytes from eight PCOS patients, there was no significant difference in fertilization, useable or total blastocyst development (day 5 or 6 embryos) and that clinical pregnancy results were identical. This research provides a greater awareness of a fertilization technique which is not normally utilized for IVM treatment, providing a less invasive, more cost-effective approach for the patient.     

苜蓿草块替代部分精料补充料对奶牛产奶性能的影响

本试验选用体重、产奶量、产奶月份、胎次均相近的健康荷斯坦产奶母牛40头,随机分成2组,即对照组和试验组,进行苜蓿草块替代部分精料补充料的饲喂试验。对照组按原日粮饲喂,试验组用1kg苜蓿苹块替代等量的精料补充料。预试期为7d,正试期35d。从奶牛的产奶量来看,与对照组比较,试验组平均每头牛每天产奶量提高了3．89％。从乳品质来看,与对照组比较,试验组的乳脂率提高了0．14个百分点,乳蛋白提高了0．09个百分点,干物质提高了0．11个百分点。通过经济效益分析比较,与对照组比较,试验组提高了10．58％。结果表明,使用苜蓿苹块替代部分精料补充料饲喂奶牛是切实可行的。     

蒽酮-硫酸比色法测定香菇多糖含量

目的：建立一种香菇多糖原料药中香菇多糖含量的测定方法。方法：以右旋糖苷为对照品,使用蒽酮-硫酸比色法测定。结果：在右旋糖苷浓度为10.004-50.020ug/ml范围内,625nm处浓度与吸光度线性关系良好,A=0.0183C-0.0019,R=0.9997（n=5）,重复性RSD=0.51%（n=6）,回收率平均值98.69%,RSD=0.50%（n=6）,与以香菇多糖精制品为对照品的测定值对比,结果良好。结论：本法简便、可行、准确,适用于香菇多糖原料药中香菇多糖含量的测定。     

缺血修饰白蛋白在急性冠脉综合征早期诊断的临床应用

目的:探讨缺血修饰白蛋白(IMA)诊断急性冠脉综合征(ACS)的价值.方法:测定37例ACS患者(ACS组)和80例体检者(对照组)血清IMA浓度.建立IMA诊断ACS的ROC曲线,计算敏感度、特异度、阳性预测值、阴性预测值.结果:健康对照组与ACS组间血清IMA浓度有显著性差异(P<0.05).IMA诊断ACS的曲线...     

水蛭仿生酶解有效部位HPLC指纹图谱的研究

目的:建立水蛭仿生酶解有效部位HPLC指纹图谱的鉴定标准。方法:采用依利特SinoChrom ODS-BP色谱柱;0～25min,甲醇(1%～10%)～水梯度洗脱;柱温35℃;流速0.8 mL.min-1;检测波长254nm。结果:在相同色谱条件下获得6批水蛭仿生酶解有效部位的指纹色谱图,其中8个色谱峰分离较好,均达到指纹图谱的技术要求。结论:HPLC指纹图谱中8个共有峰的相对保留时间比值(RRT)和相对峰面积比值(RPA)可用于水蛭仿生酶解有效部位的质量控制。     

野生型DPP Ⅳ真核表达质粒的构建和鉴定

目的构建野生型DPP Ⅳ真核表达质粒。方法将野生型DPP Ⅳ基因定向克隆到真核表达质粒pcDNA3.1(+),构建成重组质粒pcDNA3 DPP Ⅳ;然后用限制性内切酶消化和DNA序列测定鉴定。结果经酶切和DNA序列测定鉴定证实重组质粒构建正确。结论真核表达质粒pcDNA3DPP Ⅳ构建成功,为进一步研究DPP Ⅳ基因在卵巢癌细胞中的表达和生物学作用奠定了基础。     

艾灸对慢性疲劳大鼠免疫功能的影响

目的:探讨艾灸治疗慢性疲劳综合征(CFS)的可能机理。方法:采用悬吊冷水游泳复合应激因素复制慢性疲劳动物模型,随机分为正常组、模型组、艾灸组。治疗12天后作免疫功能相关指标检测,同时作免疫组化及图像分析。结果:艾灸组的脾脏指数、脾淋巴细胞增殖转化能力、血清白介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)测定值与模型组相比较P<0.01或P<0.05;艾灸组与模型组相比较,IL-2和TNF-α光密度降低,表达增强(P<0.01)。结论:艾灸能增强慢性疲劳大鼠的免疫功能,这可能是其治疗CFS的机制之一。