大黄酸对大鼠前体脂肪细胞增殖与分化的影响

 目的研究大黄酸(rhein,Rh)对大鼠前体脂肪细胞增殖与分化的影响。方法采用噻唑蓝(MTT)比色法和流式细胞术测定RH对大鼠前体脂肪细胞增殖的影响。采用油红O染色提取法,检测脂肪细胞内甘油三酯积聚量的变化;采用形态学观察,检测前体脂肪细胞充脂率、充脂程度及形态变化。结果RH在5～160μmol·L^-1内,对前体脂肪细胞增殖与分化的抑制作用呈剂量和时间依赖性变化,并可在一定程度上诱发脂肪细胞凋亡。结论Rh可抑制前体脂肪细胞的增殖与分化,具有潜在的减肥降脂作用。     

Blog和RSS环境下PBL教学设计实践

针对目前开展的PBL教学中存在对其理解上的误区和具体操作流于形式等问题，利用Blog和RSS技术来弥补在PBL教学中存在的不足，并提出了相应的教学设计模式。同时，以（Dreamweaver制作主页》为实践，对该模式进行证明，得出客观的评价和反馈信息，为信息化辅助教学提供了良好的参照。     

家庭功能对4~6岁幼儿行为问题的影响

目的探讨家庭功能对4~6岁幼儿行为问题的影响。方法采用Achenbach儿童行为量表(父母问卷)(child behavior checklist,CBCL)和家庭功能评定量表(family assessment device,FAD)对416名4~6岁幼儿父亲或母亲进行问卷调查,运用SPSS 20.0软件进行非参数检验、Spearman相关、二元Logistic回归分析。结果4~6岁幼儿家庭功能得分为[1.92(1.67,2.17)分]。Spearman相关分析结果显示,幼儿行为问题与家庭功能评定量表的沟通(r=0.102)、角色(r=0.215)、情感反应(r=0.158)、情感介入(r=0.177)和行为控制(r=0.140)维度呈正相关(均P<0.05)。Logistic回归分析显示,情感介入可预测幼儿行为问题(B=1.024,OR=2.786,95%CI=1.354~5.733)。结论家庭功能中的情感介入功能是4~6岁幼儿的行为水平的影响因素。     

The Occurrence of Core Muscle Fatigue During High-Intensity Running Exercise and its Limitation to Performance: The Role of Respiratory Work

This study investigated the occurrence of core muscle fatigue during high-intensity running exercise and its limitation to exercise performance. A secondary aim was to investigate whether respiratory muscle work performed during intense running periods, would contribute to core muscle fatigue. Nine male recreational runners were recruited for two reasons; (1) to perform a continuous treadmill run at 85% VO₂max with and without core muscle fatigue in the CR_F and CR trials, respectively; and (2) to mimic the treadmill run-induced respiratory response recorded in the CR trial while subjects were free of whole-body exercise (Mimic trial). The changes in global core muscle function with fatigue in this study were evaluated by performing a sport-specific endurance plank test (SEPT), and the associated influence on running performance was examined by comparing the time to exhaustion during the treadmill run between the CR and CR_F trials. Subsequent to the treadmill run in the CR trial, SEPT (255.7 ± 85.3 vs 177.3 ± 80.6 s) was reduced from baseline in all runners. The reduction correlated (r = 0.67) with the concomitant decline in inspiratory muscle function revealed by maximal inspiratory mouth pressure (PI_max: 151.3 ± 18.2 vs 133.3 ± 17.2 cmH₂O, p < 0.05). In the Mimic trial, similar results in SEPT (212.3 ± 90.2 s), PI_max (129.0 ± 26.7 cmH₂O), and correlation (r = 0.77, p < 0.05) were observed following voluntary hyperpneic activity. With the preceded fatigued core muscle workout in the CR_F trial, the running capacity was impaired significantly (10.7 ± 4.5 vs 6.5 ± 2.0 min, p < 0.05). The impairment was correlated (r=0.72) to the SEPT reduction resulting from the workout. The results suggest that a high-intensity maximum run may induce core muscle fatigue in runners. The core muscle fatigue, which may be partly attributed to the corresponding respiratory work, may limit their running endurance. Inspiratory muscle function appears to be essential for core stabilization during the intense running.

Key points

• A high-intensity maximum run may induce core muscle fatigue in runners. The core muscle fatigue, which may be partly attributed to the corresponding respiratory work, may limit their running endurance.
• In support of previous notion, inspiratory muscles may share the work of core stabilization during intense exercise, while simultaneously increasing the demand for breathing.
• Inspiratory muscle training incorporated into a running specific-core training regime potentially enhances the training effect on the core muscles in a functional manner to deal with the challenges faced during intense exercise.

Key words: Core stability, muscle function, respiratory muscle, plank test     

La2O2CO3:Tb3+ one-dimensional nanorod with green persistent luminescence

Trivalent terbium-doped oxycarbonate (La₂O₂CO₃:1%Tb³⁺) one-dimensional nanorods are synthesized via a facile precipitation method. The average length of La₂O₂CO₃:1%Tb³⁺ nanorods is 184.5 nm. Doping Tb³⁺ ions led to several visible emission peaks at 486 nm, 542 nm, and 587 nm under excitation of 258 nm wavelength light. The green afterglow at 542 nm can be detected almost 600 s after ceasing the UV-light irradiation. It can be calculated that the La₂O₂CO₃:1%Tb³⁺ sample has one shallow trap depth (E = 0.848 eV) by measuring the thermoluminescence. All the results indicate that a simple precipitation method can synthesize a one-dimensional nanorod with green persistent luminescence.

Trivalent terbium-doped oxycarbonate (La₂O₂CO₃:1%Tb³⁺) one-dimensional nanorods are synthesized via a facile precipitation method.     

L’hyperprolactinémie dans le syndrome de Gougerot-Sjögren est-elle une caractéristique d’un sous-groupe de patients ou fait-elle partie de la maladie ?

Objective. – To investigate the prevalence of hyperprolactinemia in patients with primary Sjogren’s syndrome (SS), its clinical significance and its implication to our understanding of the disease pathogenesis.Materials and methods. – Forty-nine patients with primary SS syndrome (44 females and 5 males) age range 37–66 years were included in this study. All patients underwent clinical assessment for disease manifestations in addition to the laboratory assessment for serum prolactin, sex hormones and immunological profile. Fifty healthy subjects (44 females and 6 males) of matched age were studied as control group.Results. – The mean prolactin serum level was significantly higher in SS patients compared to the control group (P < 0.01). This significant difference was persistent after subgrouping the patients and the controls based on their menstrual history. Hyperprolactinemia (>20 ng/ml) was prevalent in 16.3% of SS patients. There was no correlation between serum prolactin levels and hormonal status, auto-antibodies as well as systemic manifestations of the disease.Conclusion. – Patients with primary SS have moderately increased the levels of prolactin. Hyperprolactinemia reflects the disease pathology rather than being present in a subset of patients. The presence of elevated prolactin levels was not associated with hormonal status, clinical or immunological manifestations of primary SS.