某高校女教职工乳腺红外线检查结果分析

目的了解某高校女教职工乳腺红外线检查结果及患病情况,为女教职工乳腺疾病的防治工作提供参考依据。方法收集整理健康体检中541名女教职工的乳腺红外线检查结果资料并做统计学分析。结果检出乳腺疾病211人．检出率39．00％。其中乳腺小叶增生检出率19．59％,乳腺轻度增生检出率16．08％．两者均为40-岁组检出率最高,分别为30．14％、21．92％,20～岁组次之,50～岁组检出率最低;乳腺囊性或结节性增生检出率2．40％;乳腺纤维瘤或肿块检出率0．92％。结论该高校女教职工乳腺不同程度增生检出率较高．应引起高度重视并积极治疗。定期进行健康体检和开展健康教育等保健工作很有必要。     

半夏与其伪品掌叶半夏的RAPD鉴别

目的建立半夏及其伪品掌叶半夏的快速简单的分子鉴定方法。方法 SDS法提取半夏和掌叶半夏的基因组DNA,20个10碱基随机引物(S1-S20,上海生工)用于RAPD-PCR分析。结果 20个引物中有7个有效稳定的引物在半夏和掌叶半夏之间能显示出多态性指纹图谱,其中S10和S17在半夏和掌叶半夏之间显示较大差异。结论利用引物S10和S17进行RAPD-PCR能有效鉴别半夏和掌叶半夏。     

Microfluidic gradient PCR (MG-PCR): a new method for microfluidic DNA amplification

This study develops a new microfluidic DNA amplification strategy for executing parallel DNA amplification in the microfluidic gradient polymerase chain reaction (MG-PCR) device. The developed temperature gradient microfluidic system is generated by using an innovative fin design. The device mainly consists of modular thermally conductive copper flake which is attached onto a finned aluminum heat sink with a small fan. In our microfluidic temperature gradient prototype, a non-linear temperature gradient is produced along the gradient direction. On the copper flake of length 45 mm, width 40 mm and thickness 4 mm, the temperature gradient easily spans the range from 97 to 52°C. By making full use of the hot (90–97°C) and cold (60–70°C) regions on the temperature gradient device, the parallel, two-temperature MG-PCR amplification is feasible. As a demonstration, the MG-PCR from three parallel reactions of 112-bp Escherichia coli DNA fragment is performed in a continuous-flow format, in which the flow of the PCR reagent in the closed loop is induced by the buoyancy-driven nature convection. Although the prototype is not optimized, the MG-PCR amplification can be completed in less than 45 min. However, the MG-PCR thermocycler presented herein can be further scaled-down, and thus the amplification times and reagent consumption can be further reduced. In addition, the currently developed temperature gradient technology can be applied onto other continuous-flow MG-PCR systems or used for other analytical purposes such as parallel and combination measurements, and fluorescent melting curve analysis.     

What predicts the trajectory of rumination?: A prospective evaluation

The current report aimed to document individual differences that predict the trajectory of post-event rumination following an evaluative event. In this study, 127 undergraduate students were assessed over a 5-day period preceding and following a mid-term exam. Participants completed measures of anticipatory processing, trait test anxiety, trait tendency to ruminate, negative affect, and post-event rumination. Hierarchical linear modeling was used to examine predictors of post-evaluative rumination. Results suggested that individuals who reported high levels of anticipatory processing and trait test anxiety tended to experience prolonged amounts of rumination following the exam, even when controlling for negative affect, relative to those who scored low on these measures. These results suggest that specific individual difference factors impact the amount and trajectory of rumination beyond levels of general negative affect. Implications for understanding risk factors for heightened rumination are discussed, with particular attention to the larger rumination literature.