LC-MS/MS法测定猪肉中7种喹诺酮类药物的残留

目的 采用HPLC-MS/MS法测定猪肉样品中7种喹诺酮类药物的残留.方法 用乙腈提取样品基质中药物的残留,经正己烷脱脂、MCX固相萃取柱净化,以保留时间和离子对(母离子和两个碎片离子)信息比较进行定性,以母离子和响应值高的碎片离子进行定量,采用正离子MRM监测模式.结果 7种喹诺酮类药物浓度为2～ 200 μg· kg-1时与峰面积的线性关系良好,最低检测限为0.1 ～0.5 μg·kg-1,定量限为1.0～1.5 μg·kg-1;方法回收率为92％ ～ 110％,精密度较好,日内和日间RSD均小于15％.结论 所用方法分析速度快、灵敏度高、特异性好,可用于猪肉中喹诺酮类药物残留的定量和定性检测.     

A rare case of Castleman’s disease of plasma cell type within kidney

Castleman＇s disease （CD）, also known as angiofollicular lymph node hyperplasia, giant lymph node hyperplasia, and angiomatous lymphoid hyperplasia, was first described by Castleman in 1954.1 It is an uncommon disorder characterized by benign proliferation of lymphoid tissue. The etiology of CD is unclear, although its origins have been explored in various perspectives, such as immuno-depressed states, chronic inflammation or infection, and autoimmune processes.     

Laparoscopic treatment of a calcium fluorophosphate stone within a seminal vesicle cyst

Stones in the seminal vesicles are extremely rare. We present a 62-year-old patient with a stone within a seminal vesicle cyst, who was cured by laparoscopic treatment. The operative time was 80 rnin, and the estimated blood loss was 90 mL. Scanning electron microscope examination of the stone showed a compact crystal image externally and sparse spherical crystal structure in kernel. Composition of the stone was calcium fluorophosphate on X-ray diffractometer. The follow-up time was 15 months with no recurrence of cyst or stone. To our knowledge, this case is the first to describe laparoscopic removal of a stone within a seminal vesicle cyst, and the first to describe calcium fluorophosphate as the composition of seminal vesicle stones. （Asian JAndro12008 Mar; 10： 337-340）     

Imaging biomarkers for Alzheimer’s disease and glaucoma: Current and future practices

Glaucoma is a leading cause of blindness worldwide. Although intraocular pressure is the main risk factor for glaucoma, several intraocular pressure independent factors have been associated with the risk of developing the disease and its progression. The diagnosis of glaucoma relies on clinical features of the optic nerve, visual field test, and optical coherence tomography. However, the multidisciplinary aspect of the disease suggests that other biomarkers may be useful for the diagnosis, thus underling the importance of novel imaging techniques supporting clinicians.This review analyzes the common pathogenic mechanisms between glaucoma and Alzheimer’s disease and the possible novel approaches for diagnosis and follow up.     

孕早期焦虑和抑郁对妊娠期糖尿病的影响

目的探讨孕早期焦虑和抑郁对妊娠期糖尿病(gestational diabetes mellitus,GDM)的影响。方法采用前瞻性研究,于2017年通过立意抽样方法选取四川省妇幼保健院产前门诊1426名孕8～14周单胎健康孕妇为研究对象,年龄为(28.6±4.0)岁。通过问卷调查收集其年龄、孕前体重、产次、孕次等基本信息,分别采用焦虑自评量表(self-rating anxiety scale,SAS)与抑郁自评量表(self-rating depression scale,SDS)收集孕早期焦虑和抑郁信息,根据中国常模标准评价其焦虑和抑郁症状;于孕24～28周行口服葡萄糖耐量试验(oral glucose tolerance test,OGTT),根据《中国妊娠合并糖尿病诊治指南(2014)》诊断GDM。多因素非条件Logistic回归模型分析孕早期焦虑和抑郁对GDM的影响。结果孕早期焦虑组和抑郁组GDM发生率分别为41.8%和33.6%。孕早期妇女焦虑、抑郁、焦虑合并抑郁发生率分别为7.7%、10.5%和4.8%,<30岁组孕早期焦虑和抑郁发生率(9.0%和11.7%)均高于...     

股骨远端C3型骨折行对侧髂骨取骨有利于膝关节早期康复训练

目的研究对侧髂骨取骨与同侧取骨相比是否会对股骨远端C3型骨折术后膝关节功能康复产生影响。 方法按照纳入、排除标准,前瞻性纳入自2013年4月至2015年7月手术治疗股骨远端C3型骨折的病例,按照手术侧与髂骨取骨侧是否相同,分为同侧取骨组、对侧取骨组。分析两组患者的手术耗时;术后患侧膝关节主动伸直达到10°、屈曲达到120°的时间间隔;术后3个月末髂骨取骨区的疼痛视觉模拟评分(VAS);记录随访期内取骨区出现切口红肿、切口延迟愈合、血肿、感染、髂嵴撕脱骨折等情况。计量资料采用t检验或秩和检验,计数资料采用卡方检验,术后患侧膝关节主动伸直达到10°、屈曲达到120°的时间间隔采用Kaplan-Meier法进行生存分析,行Breslow检验。 结果共纳入同侧取骨组12例、对侧取骨组11例,同侧取骨组随访中位时间18(14,21)个月,对侧取骨组随访中位时间15(13,20)个月。对侧取骨组手术耗时(t＝2.169,P<0.05)、术后膝关节主动伸直达到10°、屈曲达到120°的预期时间(χ²＝7.999,P<0.05;χ²＝9.325,P<0.05)、取骨区疼痛减轻程度(t＝8.910,P<0.01)都优于同侧取骨组。术后同侧、对侧取骨组分别出现1例、2例取骨处切口红肿,予以对症治疗后好转、痊愈;所有患者随访期内均未发生取骨区血肿、感染、髂嵴撕脱骨折。 结论股骨远端C3型骨折行对侧髂骨取骨有利于膝关节早期康复训练。     

暴力性眼外伤损伤特征的调查研究

目的 调查分析暴力性眼外伤的损伤程度、部位、致伤物及致盲后遗障碍的类型。方法 对1990～1999年近10年法医临床学鉴定案例中的暴力性眼外伤369例进行分析。结果 暴力性眼外伤受害者中男302例，女67例。伤害主要发生于公共场所、乡村及农场。致伤物中以钝器尤以拳掌多见，伤眼中左眼多于右眼。损伤部位以眼睑184例为多见。损伤程度：轻度损伤以单纯眼睑擦伤175例为多见，中度损伤以外伤性瞳孔散大76例和前房积血76例为多见，重度损伤有角巩膜穿孔伤76例和外伤性自内障83例为多见。致盲病变中角膜瘢痕、外伤性自内障分别列第一、二位。结论 暴力性眼外伤的损伤特征研究有利于从多方面综合预防眼外伤，降低眼外伤的发生率。     

VEGFA、VEGFC及其受体和angiopoietin-1/angiopoietin-2及其受体在人胚胎卵黄囊血岛、AGM区的表达

本研究观察VEGFA、VEGFC及其受体和angiopoietin-1、angiopoietin-2及其受体在发育第3-12周人胚胎卵黄囊血岛、主动脉-生殖腺-中肾(AGM)区的表达。在征得意外流产孕妇同意并签字后，收集其意外流产的受精龄第3-12周的人胚胎，用4％多聚甲醛固定，石蜡包埋切片，HE和免疫组织化学染色，光镜观察。结果表明：第21-25天人胚卵黄囊血岛血管内皮细胞和造血细胞强表达VEGFA及其受体flt1和flk-1，VEGFC及其受体fit4，anglopomtin-2及其受体Tie-2蛋白；弱表达angiopoietin-1蛋白。背主动脉和中肾于发育第4周开始表达上述各种因子及其受体，免疫阳性反应物集中在大而圆、有核的造血细胞。阳性细胞的数量到第7周到达高峰。8周以后，阳性细胞数量显著减少，到12周几乎所有的血细胞为免疫阴性反应。生殖腺主要在第6—8周表达VEGFA，flt1，flt4，angiopoietin-1，angiopoietin-2和Tie-2蛋白，但不表达VEGFC和flk-1。在AGM区的血管内皮细胞未检测到上述各因子的表达。结论：人胚胎卵黄囊血岛造血细胞和内皮细胞以及背主动脉和中肾共表达多种与血管发生和血液发生相关的因子。人胚内造血发生于第4周。     

Effect of Chaiqinchengqi decoction on serum amyloid A in severe acute pancreatitis patients

ObjectiveTo investigate the effect of Chaiqinchengqi decoction (CQCQD) on serum amyloid A (SAA) in severe acute pancreatitis (SAP) patients.MethodsThirty-five participants enrolled and were randomly assigned into either a treatment condition (n = 17, treated with CQCQD) or a control condition (n = 18, treated with placebo) 24 hours following the onset of the disease. No statistical difference was observed in either group at baseline. Upon admission, the Acute Physiology and Chronic Health Evaluation score II (APACHE II), SAA, serum C-reactive protein (CRP) and interleukin-6 (IL-6) were measured, as well as on the first, 3rd and 7th day and were compared between the two groups. Organ complications, infection, operation rate, mortality and hospital stay were also compared.ResultsThe duration of acute respiratory distress syndrome, acute hepatitis, acute renal failure, gastrointestinal failure and blood coagulation dysfunction were shorter in the treatment group than in those in the control group (P < 0.05). The secondary infection rates and the hospital fees in the treatment group were lower than those in the control group (P < 0.05) as well as length of hospital stay (P < 0.01). After 3 days of hospitalization, the APACHEII, score SAA levels, serum CRP and IL-6 in the treatment group was lower than those in the control group (P < 0.05). SAA was positively correlated with serum CRP (R = 0.346, P = 0.042), Ranson score (R = 0.442, P = 0.008) and serum IL-6 (R = 0.359, P = 0.034). The area under the receiver operating characteristic curve of admission SAA predict pancreatic necrosis (PN) was 0.815 (95% CI: 0.625–0.954; P = 0.006). The best cut-off value of admission SAA was 7.85 mg/L with the sensitivity 84.6% and specificity 68.2%.ConclusionsThe CQCQD can reduce the duration of organ damage through lowering the SAA in SAP patients and the SAA can early predict the PN and severity of SAP patients.