人T-bet基因的克隆及序列分析

目的：克隆人T-bet基因全长编码区的cDNA，并进行豁定和测序分析，为从转录水平研究Th1／Th2平衡及其与免疫相关性疾病发生发展的关系奠定基础。方法：采用RT-PCR方法，从人外周血淋巴细胞获得T-bet基因的cDNA；连接至pGEM-Teasy载体，导入大肠杆菌DH5α并选择阳性克隆；应用M13F／R通用引物进行双向反应测序，以作序列鉴定。结果：扩增得到的T-bet基因cDNA全长1670 bp，包括编码区1607 bp，编码530个氨摹酸残基，与Genbank中发表的序列完全一致。结论：获得了人T-bet基网的克隆，为进一步研究其生物学功能构建了基础平台，为寻找多种免疫性疾病的有效治疗提供新思路。     

腹水浓缩回输腹腔治疗顽固性腹水23例报告

目的：评价腹水浓缩回输腹腔治疗顽固性腹水的疗效。方法：23例顽固性腹水患者，在原治疗基础上加用腹水浓缩回输治疗，观察腹水浓缩回输前后体重、腹围、尿量、血浆总蛋白、白蛋白、血中电解质的变化。结果：治疗后所有患者体重下降，腹围减少，尿量增加（P＜0．01）；血浆总蛋白、白蛋白均升高（P＜0．05）；而血中电解质治疗前后无显著变化（P＞0．05）。结论：腹水浓缩回输腹腔对治疗顽固性腹水有良好的效果。     

哮喘患儿血浆IL-4测定及其意义

目的:观察哮喘患儿血浆IL-4水平,了解哮喘患儿的免疫学特征。方法:检测哮喘患儿血浆IL-4、IgE含量,对血浆IL-4与血浆总IgE进行相关性分析;ELISA法测定血浆IL-4和血浆总IgE。结果:①实验组血浆IL-4水平为115.8±48.5 pg/m l,较正常对照组儿童血浆IL-4水平38.3±10.0 pg/m l明显增高,两组差异显著(P<0.01)。②实验组血浆总IgE水平为185.3±51.3 kU/L,较正常对照组儿童血浆总IgE水平45.6±16.0 kU/L明显增高,两组差异显著(P<0.01)。③血浆IL-4与血浆总IgE水平呈正相关,r=0.693,差异显著(P<0.01)。结论:哮喘患儿血浆IL-4较正常儿童明显增高,血浆IL-4检测对于哮喘患儿诊断治疗有重要意义。     

苦瓜多糖对链脲佐菌素诱导糖尿病小鼠的降血糖效果

目的:研究苦瓜水提多糖(watersolublepolysaccharidesofMomordicacharantiaL.MCW)和苦瓜碱提多糖(alkalinesolublepolysaccharidesofMomordicacharantiaL.MCB)对STZ诱导糖尿病小鼠的降血糖作用。方法:昆明种小鼠120只,随机抽取12只作空白对照,余下108只,禁食后于次日晨腹腔注射链脲佐菌素(STZ)200mg/kgbw。取血糖值在11.1mmol/L以上者随机分组,共4组:模型组、MCW组(300mg/kgbw)、MCB组(300mg/kgbw)和优降糖组(15mg/kgbw)。连续灌胃10d后,禁食,次日晨眼眶取血,测定血糖及血清胰岛素含量。解剖取胰腺,观察小鼠胰岛组织形态。结果:MCW和MCB都可以降低糖尿病小鼠空腹血糖,MCB组分的降血糖效果最佳,不但可以显著降低糖尿病小鼠空腹血糖值,而且可以提高糖尿病小鼠血清胰岛素水平。结论:苦瓜多糖能帮助恢复受损胰岛组织,促进胰岛素的分泌,从而降低血糖。     

铽离子增敏环丙沙星研究及其应用

 目的建立胶囊和血样中环丙沙星的含量测定方法。方法在醋酸-醋酸钠缓冲液(pH6.0)中,铽(Tb³⁺))能与环丙沙星(ciprofloxacin)形成络合物,该络合物吸收紫外光后,能发射铽的特征荧光。λ_ex/λ_em=325.0/545.0,狭缝均为5nm。结果线性范围1.0×10^-8～1.0×10^-6mol·L^-1。回归方程为F=8.26×10⁸c+18.5(r=0.9999)(c:mol·L^-1);检出限为3.5μg·L^-1。结论该法用于血清和胶囊中环丙沙星的含量测定,简单、快速、灵敏,结果令人满意。     

EFFECT OF ELECTROACUPUNCTURE AND CALCIUM-CHANNEL INHIBITORS ON CYTOPLASMIC FREE CALCIUM CONCENTRATION OF MOUSE BRAIN CELLS

Ithasbeenrecognizedthattheunivalentandbi valentionsareimportanttothenormalactivitiesof thenervoussystem,especially,Ca2+playsakeyrole inthestabilityandpermeabilityofneurilemma,the transmissionofnerveimpulses,releaseoftransmitters ofthenerveendingsandtheirresultantbiologicef fectsthroughthepost synapticmembrane,generation andaftereffectsofthesecondmessenger,etc.[1,4].Manyresearcheshaveshowedthattheanalgesiceffect ofmorphine likedrugsandendogenousmorphine like substances(endorphin,etc)isrealizedb…     

HPLC法同时测定血浆地西泮及其代谢物浓度

目的 :建立同时测定血浆中地西泮及其代谢物浓度的方法。方法 :选用ZORBAXRP C18柱 (15 0mm× 4 6mm ,5 μm) ;甲醇 - 2 5mmol·L-1醋酸铵溶液 (6 0∶4 0 ,V/V)作流动相 ;流速 0 8mL·min-1;检测波长 2 30nm。取血浆样品 0 5mL ,在碱性条件下用二氯甲烷 -正己烷提取 ,HPLC检测。结果 :本法对替马西泮、去甲地西泮和地西泮 3种物质的最低检测限均为 2 μg·L-1,线性范围为 10～ 15 0 0 μg·L-1;奥沙西泮的最低检测限为 5 μg·L-1,线性范围为 2 0～ 15 0 0 μg·L-1。回收率均接近 10 0 % ,日内、日间RSD <5 %。结论 :本法能同时测定血浆中地西泮及其代谢物浓度 ,具有重现性好 ,灵敏、可靠 ,可用于地西泮中毒的监测     

新旧《医院财务制度》衔接的体会

镇江是全国两轮医改的试点城市。文章从6个方面总结镇江四院率先执行新《医院财务制度》所取得的经验,为全国顺利接轨服务。     

A possible significant role of zinc and GPR39 zinc sensing receptor in Alzheimer disease and epilepsy

Zinc the essential trace element, plays a significant role in the brain development and in the proper brain functions at every stage of life. Misbalance of zinc (Zn²⁺) ions in the central nervous system is involved in the pathogenesis of numerous neurodegenerative disorders such as Alzheimer's disease, Depression, and Epilepsy. In brain, Zn²⁺ has been identified as a ligand, capable of activating and inhibiting the receptors including the NMDA-type glutamate receptors (NMDARs), GABA_A receptors, nicotinic acetylcholine receptors (nAChRs), glycine receptors (glyR) and serotonin receptors (5-HT3). Recently GPR39 has been identified as a zinc-specific receptor, widely expressed in brain tissues including the frontal cortex, amygdala, and hippocampus. GPR39, when binding with Zn²⁺ has shown promising therapeutic potentials. This review presents current knowledge regarding the role of GPR39 zinc sensing receptor in brain, with a focus on Alzheimer’s disease and Epilepsy. Although the results are encouraging, further research is needed to clarify zinc and GPR39 role in the treatment of Alzheimer's disease and Epilepsy.     

Continuous and online analysis of heart rate variability

Spectral analysis of heart rate variability provides a probe to assess the function of the sympathetic and parasympathetic nervous systems. Time-frequency analysis of heart rate variability is useful for investigating autonomic nervous function in patients with syncope or non-sustained ventricular tachycardia, or in anaesthesia, etc. In this paper, we developed an algorithm for continuous and online analysis of heart rate variability. The algorithm was simulated and evaluated in MATLAB, and implemented on the digital signal processor. The electrocardiogram signals from MIT/BIH arrhythmia database and one patient with syncope demonstrate the capability of the proposed method in the continuous and online analysis of heart rate variability.