槲皮素的结构修饰及生物活性研究进展

槲皮素是蔬菜、水果、中药中常见的黄酮类化合物,是天然的抗氧化剂,具有抗癌、抗糖尿病、抗菌、抗炎、抗病毒等生物活性。由于槲皮素生物利用度低,限制了其在临床上的应用。通过各种方法设计和合成新的槲皮素衍生物,以改善其缺点,进而发挥预防和治疗疾病的作用。通过对槲皮素衍生物的合成及其抗癌、抗糖尿病、抗炎、抗菌和抗病毒活性进行综述,并对其构效关系进行分析,为天然化合物的开发和利用奠定基础。     

Overlapping obesity-related glomerulopathy and immunoglobulin A nephropathy: clinical and pathologic characteristics and prognosis

Clinical and Experimental Nephrology - In this study, we investigated the clinical and pathologic characteristics and prognosis of overlapping obesity-related glomerulopathy (ORG) and...     

How do the Growth and Metabolic Activity of Aquatic fungi Geotrichum Candidum and Aspergillus Niger Respond to Nanoplastics?

In this study, exposure experiments were conducted to assess the effects of polystyrene nanoparticles (PS) and amine-modified polystyrene nanoparticles (APS) at environmental concentrations (1, 10, and 100 µg L^??1) on two fungal species (Geotrichum candidum and Aspergillus niger), isolated from leaf litter in streams, concerning their growth and metabolic activity. Results showed that PS at 1 and 10 µg L^??1 have hormesis effects on G. candidum growth. Compared with G. candidum, A. niger had higher sensitivity to nanoplastic exposure. Besides, the peroxidase and cellobiohydrolase activities of A. niger were significantly inhibited by nanoplastics (except 1 µg L^??1 PS), which would weaken its metabolic activity in carbon cycling. These results provided a new thought on how the growth and functions of aquatic fungi cope with the stress induced by nanoplastics. Overall, the study provided evidence for the different responses of aquatic fungi to nanoplastics in streams.

     

热化疗对人体结肠癌LoVo细胞中一氧化氮含量的影响

Aim To study the effect of nitric oxide (NO) on LoVo cells after hyperthermia combined with MMC and their mechanisms. Methods The levels of NO were determined in cell culture medium of LoVo cells by Cu-Cd reduction method.Results The levels of NO in control (45.9±16.12) μmol/L group were lower than those in chemohyperthermy(92.3±24.30) μmol/L and hyperthermia (67.67±15.48)μmol/L group (P＜0.01,P＜0.05 respectively). Levels of NO in chemohyperthermy and hyperthermia groups were higher than those in chemotherapy(47.03±16.32)μmol/L(P＜0.01,P＜0.05 respcevtively). The levels of NO in chemo-hyperthermy group were higher than those in hyperthermia group(P＜0.05), and those in chemotherapy group higher than in control group but without statistical significance.Conclusion Chemohyperthermy and hyperthermia could remarkably enhance the levels of NO produced by LoVo cells. NO levels could also be enhanced by chemotherapy. It could be concluded that Nitric Oxide Synthase (NOS) in tumor cells might be activated by hyperthermia, chemohyperthermy and chemotherapy, and increased NO level which induced cytotoxic reaction. This may be one of the mechanisms of treatment with chemohyperthermy.     

视网膜母细胞瘤中Fas和bcl-2的表达及意义

目的　探讨Fas及bcl 2在视网膜母细胞瘤的表达及意义。方法　用免疫组织化学SP方法检测了 45例视网膜母细胞瘤及 12例正常视网膜组织中Fas及bcl 2蛋白的表达 ,并进行比较。结果　Fas在视网膜母细胞瘤组织及正常视网膜组织的表达分别为 0 .2 5 5± 0 .441,0 .0 83± 0 .2 89,两者比较差异无显著性 (P =0 .114 8) ,均呈低表达 ;bcl 2在视网膜母细胞瘤中的表达为2 .70 2± 1.0 82 ,与正常视网膜组织中 0 .16 7± 0 .389相比差异具非常显著性 (P <0 .0 0 0 1)。结论　Fas在视网膜母细胞瘤及正常视网膜中均呈低表达 ;而bcl 2在视网膜母细胞瘤中呈明显高表达 ,其可明显抑制凋亡的发生 ,与肿瘤的发展关系密切。     

U-Patch GAN: A Medical Image Fusion Method Based on GAN

Journal of Digital Imaging - Although medical imaging is frequently used to diagnose diseases, in complex diagnostic situations, specialists typically need to look at different modalities of image...     

急性非淋巴细胞白血病中MGMT与p53基因突变的研究

目的 探讨肿瘤抑制基因ｐ５３、ＭＧＭＴ在急性非淋巴细胞白血病（ＡＮＬＬ）中的改变。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术对３１例ＡＮＬＬ患者、２２例正常人ｐ５３基因第５、６、７、８外显子和ＭＧＭＴ基因第１、４外显子进行突变分析。结果 ３１例ＡＮＬＬ中５例存在ｐ５３基因突变、４例存在ＭＧＭＴ基因突变,其中２例ｐ５３与ＭＧＭＴ同时发生突变。结论 ｐ５３基因突变在白血病发生中起一定     

8—Br—cAMP对人视网膜母细胞瘤HXO—Rb44细胞癌基因的表达效应

目的 研究８－Ｂｒ－ｃＡＭＰ对培养的人视网膜母细胞瘤ＨＸＯ－Ｒｂ４４细胞癌基因表达的效应及其与该细胞生长的关系。方法 ｃ－ｆｏｓ ｍＲＮＡ,Ｎ－ｍｙｃ ｍＲＮＡ及ｐ２１ｒａｓ ｍＲＮＡ均以原位杂交ＲＮＡ斑点印迹技术检测,对繁殖细胞核抗原,ｖ－Ｆｏｓ,Ｎ－Ｍｙｃ和Ｐ＾２１ｒａｓ蛋白表达的免疫反应性则采用免疫组化及收白质斑点印迹技术检测。     

细菌内毒素对缺氧缺血性脑损伤新生大鼠远期智能的影响

目的：探讨细菌内毒素脂多糖（LPS）对缺氧缺血性脑损伤（HIBD）新生大鼠远期智能的影响，方法，建立新生大鼠LPS＋HIBD模型，于新生大鼠缺氧前4h给予腹腔注射LPS0．3mg/kg,HIBD组和对照组仅给予腹腔注射生理盐水，采用迷宫实验观察远期学习记忆能力的改变。结果：LPS＋HIBD组学习记忆能力明显低于HIBD组，表现在达标所需反映次数明显高于HIBD组（P＜0．05），而正确率支明显低于HIBD组（P＜0．05），结论：LPS对缺氧缺血引起的脑损伤有增敏作用，可进一步降低HIBD后的学习和记忆能力。