大承气汤治疗急性胰腺炎肠屏障功能障碍

目的观察大承气汤对急性胰腺炎肠屏障功能障碍的临床疗效。方法将60例急性胰腺炎中医辨证属于腑实热结证患者随机分为治疗组和对照组,各30例,在常规西医治疗基础上,设立大承气汤组为治疗组,未使用大承气汤组为对照组,系统观察患者腹痛腹胀及恶心呕吐缓解时间、胃肠道功能恢复时间,血常规、血淀粉酶及C-反应蛋白（CRP）恢复正常的时间。结果治疗组腹痛腹胀缓解时间、恶心呕吐缓解时间、胃肠道功能恢复时间及血常规、血淀粉酶、CRP恢复正常时间均较对照组显著降低,2组比较,P〈0.05。结论大承气汤治疗急性胰腺炎肠屏障功能障碍,能明显改善患者临床症状,减轻痛苦,缩短病程及住院时间,改善微循环障碍,减少组织损伤。     

辛伐他汀对冠心病患者N端脑利钠肽水平影响的分析研究

目的评价辛伐他汀对冠状动脉粥样硬化性心脏病（冠心病）患者血浆N端脑利钠肽（NT-proBNP）水平的影响。方法随机选取冠状心病患者80例为治疗组,常规治疗基础上给予辛伐他汀20mg,每晚顿服;另80例冠心病患者为对照组,仅给予常规治疗。于治疗前、治疗后8周分别测定N端脑利钠肽。结果两组患者治疗前后NT-proBNP水平比较,差异有统计学意义。结论辛伐他汀可以显著降低冠心病患者NT-proBNP水平,改善心功能。     

慢性心力衰竭患者D-二聚体和纤维蛋白原浓度的变化趋势及意义

目的探讨慢性心力衰竭患者不同心功能分级状态下D-二聚体和纤维蛋白原浓度的变化趋势及意义。方法选择2009年住院患者89例,基础疾病为高血压病、冠心病、糖尿病、瓣膜病、心肌痛,心功能按NYHA分级分为Ⅰ～Ⅳ级;采用酶联免疫吸附法测定D-二聚体和纤维蛋白原浓度,同时应用多普勒超声心动图检测左室射血分数（LVEF）、左室舒张末内径（LVD）、左房内径（LAD）。结果D-二聚体和纤维蛋白原浓度：心功能Ⅱ、Ⅲ、Ⅳ级显著高于心功能Ⅰ级（P〈0.01）,心功能Ⅳ级显著高于心功能Ⅱ级（P〈0.01）;D-二聚体和纤维蛋白原浓度变化与患者左房内径、左室舒张内径成正相关,与左室收缩功能EF呈负相关。结论慢性心力衰竭患者存在高凝状态、继发纤溶,随着心衰程度加剧而加重;并且与左房、左室的结构重构有相关性;而且D-二聚体和纤维蛋白原可用于心衰病情评估和危险分层。     

MDM2 rs2279744 polymorphism and endometrial cancer: a meta-analysis

Case–control studies on the association between mouse double minute 2 homolog (MDM2) rs2279744 polymorphism and endometrial cancer have provided either controversial or inconclusive results. To clarify the effect of MDM2 rs2279744 polymorphism on the risk of endometrial cancer, a meta-analysis of all case–control observational studies was performed. Pooled odds ratios (ORs) for various polymorphisms were estimated using random and fixed effect models. Q-statistic was used to evaluate the homogeneity, and Egger and Begg tests were used to assess publication bias. Overall, the MDM2 rs2279744 polymorphism was associated with a risk of endometrial cancer (OR?=?0.76; 95 % CI?=?0.64–0.90 for allele contrast, p?=?0.002, P _het?=?0.003). The contrast of homozygotes and the recessive and dominant models produced the same pattern of results as the allele contrast. In the analysis stratified by ethnicity, significant associations were found in the Caucasian population in all of the genetic models. Our pooled data suggest evidence for a major role of MDM2 rs2279744 polymorphism in the carcinogenesis of endometrial cancer, especially among Caucasian populations.     

#85 Association of serum antibodies to the helicobacter pylori caga antigen with precancerous gastric lesions in chinese populations with contrasting gastric cancer rates

PURPOSE: To assess whether serologic markers of Helicobacter pylori infection can account for the differences in prevalence of advanced precancerous gastric lesions (APGL) between two rural Chinese villages.METHODS: We studied asymptomatic adult subjects in Bei Duan village (N = 196) in Linqu County (a high-risk area for gastric cancer) and in Shi Huang village (N = 192) in Cangshan County (a low-risk area for gastric cancer) in Shandong Province, China. Prevalence of APGL was assessed by microscopic examination of endoscopic stomach biopsies. Enzyme-linked immunosorbent assays (ELISA) were used to detect IgG to the H. pylori whole-cell antigen, and to the CagA protein. A logistic regression model was used to quantify the role of the two H. pylori seromarkers in explaining the differences in prevalence of APGL between the two villages, after adjusting for age and sex.RESULTS: The prevalence of APGL was much greater in Bei Duan than in Shi Huang. Although H. pylori seroprevalence by the whole-cell ELISA was similar in the two populations, seroprevalence of CagA was significantly greater in Bei Duan. Although age, sex, and both H. pylori seromarkers were associated with APGL in the logistic regression model, the effect of village of residence remained strong after adjustment for all four covariates.CONCLUSION: Only a relatively small proportion of the variation in prevalence of APGL in the rural Chinese population can be explained by differences in H. pylori seroprevalence, or by differences in CagA seroprevalence.     

Cyclin D1基因多态性和儿童急性淋巴细胞性白血病相关性研究

目的 探讨细胞周期素 D1(Cyclin D1)基因A870G多态性和儿童急性淋巴细胞白血病之间的相关性.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测118例急性淋巴细胞白血病患儿(实验组)和160例健康儿童(对照组)的Cyclin D1 A870G基因型,比较两组基因型频率和等位基因频率.结果 实验组基因型频率为AA型30.51%、AG型50%、GG型19.49%,对照组基因型频率为AA型20.63%、Aa型47.5%、GG型31.67%,差异均有统计学意义(P<0.05).实验组等位基因A、G的分布频率依次为55.51%、44.49%,对照组等位基因A、G的分布频率依次为44.38%、55.62%,差异均有统计学意义(P<0.05).B细胞型患儿基因型频率为AA型50%、AG型41.67%、GG型8.33%,T细胞型患儿基因型频率为AA型25.53%、AG型52.13%、GG型22.34%,差异有统计学意义(P<0.05).结论 Cyclin D1基因A870G位点多态性与儿童急性淋巴细胞白血病发病的易感性存在相关性.     

原发性肾透明细胞癌根治术患者预后与CD99基因表达的关系及预后影响因素分析

目的探讨影响原发性肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)根治术患者的预后与肿瘤转移相关基因CD99的关系及预后的影响因素。方法应用半定量RT-PCR技术研究CD99基因在ccRCC组织和癌旁组织中的表达,通过随访调查患者的预后、生存时间及其危险因素,应用Cox风险回归模型分析获得影响预后的主要危险因素。结果与对应癌旁组织相比,CD99基因在ccRCC组织中表达上调的占73.5%,差异有统计学意义(P=0.000)。应用Cox风险回归模型分析发现:ccRCC组织中CD99基因的高表达[HR=0.14,95%CI(0.01,2.15)]与根治术患者预后的生存时间无关;而患者年龄[HR=1.18,95%CI(1.01,1.38)]、TNM分期[HR=51.91,95%CI(4.31,625.87)]、合并糖尿病[HR=59.94,95%CI(2.21,1 627)]和合并高血压[HR=47.72,95%CI(1.37,1 670)]是影响患者预后生存时间的主要危险因素。比较TNMⅠ期和Ⅱ～Ⅳ期两组患者的1年生存率和2年生存率(100.0%vs 60.0%,P=0.004;93.8%vs 8.3%,P=0.000),差异有统计学意义。结论肿瘤转移相关基因CD99的表达可能与ccRCC的预后无关,年龄、TNM分期、合并糖尿病和合并高血压是影响患者预后生存时间的主要危险因素。     

大黄有毒物质基础的研究

目的：进行大黄体内有毒物质基础的研究。方法：以病理切片和血清生化为指标,对大黄总提取物组、大黄游离葸醌组、大黄结合蒽醌组、大黄总蒽醌组、大黄鞣质组和大黄多糖组进行小鼠体内毒性实验。结果：大黄各蒽醌组尤其是游离蒽醌组对小鼠主要毒性靶器官为肾脏,而鞣质部位则有潜在的肝毒性。结论：大黄中鞣质及蒽醌类成分有潜在肝肾毒性。     

GC法测定消炎止痒醑中薄荷脑的含量

目的建立GC法测定消炎止痒醑中薄荷脑的含量。方法采用CP-Sil 5 CB色谱柱(25m×0.32mm×0.4μm),FID检测器。进样口温度250℃;柱温280℃(程序升温:初始温度60℃,保持1min,再以12℃/min的速度升至280℃,保持3min);检测器温度300℃;分流进样,分流比20:1;进样量1μL。结果薄荷脑进样量在50.8～609.6mg/L范围内与峰面积呈良好线性关系,r=0.999 69,平均回收率为99.10%,RSD为0.87%(n=6)。结论本测定方法简便、快速、准确、重复性好,可作为消炎止痒醑的含量测定方法。     

消癌解毒方诱导人肝癌SMMC-7721细胞miRNA表达变化

目的:探讨消癌解毒方对人肝癌SMMC-7721细胞增殖及微小核糖核酸(microRNA,miR)-25-3p,miR-29a-5p,miR-122-3p,miR-124-3p,miR-182-5p表达谱的影响。方法:将12只雄性大耳白兔随机分为4组,分别为消癌解毒方高、中、低剂量(15.12,7.56,3.78 g·kg-1)组和空白组,各组分别给予消癌解毒方及生理盐水灌胃4 d。4 d后于颈动脉中取血清并配制培养基。用消癌解毒方(15.12,7.56,3.78 g·kg-1)含药血清及空白血清的培养基处理人肝癌细胞株SMMC-7721,噻唑蓝(MTT)比色法检测肿瘤细胞增殖活性、实时荧光定量逆转录聚合酶链式反应(Real-time polymerase chain reaction,Real-time PCR)法验证人肝癌细胞SMMC-7721中miR-25-3p,miR-29a-5p,miR-122-3p,miR-124-3p和miR-182-5p的表达水平。结果:经过12,24,48 h后,消癌解毒方(15.12,7.56,3.78 g·kg-1)含药血清对人肝癌SMMC-7721细胞的增殖均存在抑制作用。随着消癌解毒方含药血清浓度的增加,其对人肝癌细胞SMMC-7721细胞增殖的抑制率也相应增加。其中高剂量组含药血清的抑制效果最好,其干预12,48 h的吸光度A较同期空白组明显降低(P0.05)。高剂量组干预24 h A比同期空白组显著降低(P0.01),该组抑制率为42.86%。Real-time PCR证实消癌解毒方(15.12,7.56,3.78 g·kg-1)含药血清的培养基能诱导miR-25-3p,miR-182-5p表达下调,诱导miR-29a-5p,miR-122-3p,miR-124-3p表达上调。且高剂量组的调控作用较空白组更显著(P0.01)。结论:消癌解毒方可能通过诱导miRNA表达谱的改变而参与抑制人肝癌细胞SMMC-7721增殖作用,但其具体机制仍有待进一步研究。