铜对大鼠学习记忆和海马区超微结构的影响

目的观察铜负荷饮食对大鼠空间学习记忆行为和海马超微结构的影响。方法 40只雄性大鼠随机分为模型组和空白对照组各20只,采用Morris水迷宫实验测试大鼠学习记忆能力,采用原子吸收法检测肝、脑组织铜元素含量,电镜技术观察海马区超微结构变化。结果 Morris水迷宫实验:模型组大鼠在定位航行试验中平均逃避潜伏期较正常对照组显著延长,空间探索试验中穿越平台次数较正常组明显减少(P<0.01),与正常对照组比较,模型组大鼠肝、脑组织铜含量明显增加(P<0.01),海马组织内模型组大鼠神经元突触间隙明显变宽(P<0.05),突触后致密物厚度明显变薄(P<0.05)。结论铜可能通过损伤海马超微结构引起大鼠学习记忆功能障碍。     

夏慈消结散质量标准研究

目的建立夏慈消结散的质量标准。方法 2021年 4—8月,采用薄层色谱法( TLC)对夏慈消结散中夏枯草、酒白芍、穿心莲、莪术进行了薄层色谱鉴别,采用高效液相色谱法( HPLC)测定芍药苷、迷迭香酸以及穿心莲内酯的含量。结果夏枯草、酒白芍、穿心莲、莪术的薄层色谱鉴别,供试品与对照品薄层色谱相应位置上显相同颜色的斑点、分离度好、专属性强;高效液相含量测定中芍药苷、迷迭香酸、穿心莲内酯的线性范围分别为 0.12~6.00 μg(r=0.999 9)0.07~3.50 μg(r=0.999 6)和 0.25~12.50 μg(r=0.999 9),平均回收率分别为 102.90%、101.19%和 101.90%,相对标准差( RSD2.70%、2.64%和 2.97%。结论)为建立的薄层色谱鉴别和高效液相色谱含量测定,方法简便、可行,可用于夏慈消结散的质量控制。     

通窍活血汤对血管性痴呆大鼠海马组织乙酰胆碱酯酶和胆碱乙酰转移酶含量的影响

目的:探究通窍活血汤(TQHXD)对血管性痴呆(VD)大鼠学习记忆的作用与给药时间、剂量的关系及其对海马组织中乙酰胆碱酯酶(Ach E)和胆碱乙酰转移酶(Ch AT)含量的影响。方法:将90只SD大鼠分为TQHXD高、中、低剂量组(12.04,6.02,3.01 g·kg-1)、阳性药尼麦角林(6 mg·kg-1)、模型组及假手术组。通过改良的双侧颈总动脉结扎法(2-VO)建立血管性痴呆大鼠模型,通过灌胃给予通窍活血汤12.04,6.02,3.01 g·kg-1及阳性药尼麦角林(6 mg·kg-1),每日1次,共31 d。采用Morris水迷宫实验对大鼠学习记忆能力进行检测,酶联免疫吸附试验(ELISA)方法检测大鼠海马组织中Ach E和Ch AT的含量。结果:与模型组相比,给药14,21,28 d后,TQHXD 12.04,6.02,3.01 g·kg-1能显著减少VD大鼠逃避潜伏期(EL)和平台所在象限游动的距离,结果存在显著性差异(P<0.05或0.01),其结果与给药时间及剂量呈现较好的正相关;并能提高海马组织中Ch AT的含量和降低Ach E的含量(P<0.05或0.01)。结论:通窍活血汤能够改善血管性痴呆大鼠的学习、记忆能力并呈现一定的时间、剂量依赖性,Ach E的含量降低和Ch AT的含量升高可能是促进大鼠学习记忆能力恢复的原因之一。     

Is brace necessary after cervical surgery: A meta-analysis of randomized controlled trials

Background:Currently, there are increasing surgical treatments for neck pain. However, whether to use cervical brace after operation remains poorly defined. We aim to clear the clinical efficacy of the use of cervical brace after cervical surgery.Methods:We searched for relevant studies in 8 electronic databases up to March 2021. The mean difference and 95% confidence intervals were used for continuous data. Cochrane Collaboration’s tool was used to assess the risk of bias. The data were collected and input into the Review Manager 5.3 software (The Cochrane Collaboration, Copenhagen, Denmark).Results:Four randomized controlled trials were finally included in our study. For pain, the pooled analysis showed that postoperative neck brace compared with no brace can relieve neck pain at all follow-up periods except 6 months. For neck disability index, the result showed that postoperative neck brace compared with no brace can improve neck disability index during the 3 to 12 month follow-up period. However, no significant difference was identified between 2 groups within the follow-up of 6 weeks after surgery. In addition, the result tends to get the opposite at follow-up of 24 months. For 36-Short form health survey Physical Component Summary, there was no significant difference between 2 groups in the early 3 weeks after surgery, but the results were changed after 3 weeks. For 36-short form health survey Mental Component Summary, there appears to be no significant change between 2 groups at all time intervals.Conclusion:Wearing a cervical brace after cervical surgery is conducive to improving symptoms after cervical surgery at different stages. However, there is no relevant evidence indicating it can improve the mental health of postoperative patients. Higher quality, large prospective randomized studies are needed to verify the current conclusions.     

Constructing novel molecular subtypes and an 11-gene signature based on pyroptosis signaling for lung adenocarcinoma

Pyroptosis plays important roles in various cancers. In this study, we focused on lung adenocarcinoma (LUAD) and aimed to develop new molecular subtypes based on pyroptosis signaling. Pyroptosis-related genes were used as a basis to classify molecular subtypes through unsupervised consensus clustering. Gene set enrichment analysis was performed to characterize tumor microenvironment (TME) and functional pathways. Univariate Cox regression and least absolute shrinkage and selection operator (LASSO) analysis were conducted to identify prognostic genes for establishing a prognostic model. Three molecular subtypes were established with distinct overall survival, TME and enriched pathways. C3 subtype had the longest survival and the highest immune infiltration. 11 prognostic genes were screened to build a prognostic signature for predicting LUAD prognosis. This study emphasized the important role of pyroptosis in LUAD development. Pyroptosis was considered to play critical roles in regulating TME. Moreover, the 11-gene signature could serve as an indicator for predicting LUAD prognosis, and was potential targets for developing targeted drugs.     

免疫检查点抑制剂联合抗血管生成药物治疗晚期非鳞状细胞非小细胞肺癌的研究进展

评价重组人血管内皮抑制素联合阿法替尼及替吉奥二线治疗晚期肺鳞癌患者的有效性和安全性。

共25例驱动基因阴性晚期肺鳞癌患者被纳入这项单臂前瞻性研究,按期二线给予入组患者重组人血管内皮抑制素联合阿法替尼及替吉奥治疗,观察分析患者无进展生存期(progression-free-survival,PFS)、总生存期(overall survival,OS)、疾病控制率(disease control rate,DCR)、客观缓解率(objective response rate,ORR)和不良反应(adverse reaction,AR)。

25例入组患者接受了≥2个周期的二线治疗方案,随访时间截至2023年3月31日,其中4例患者病情部分缓解,17例患者病情稳定,4例患者病情进展。经研究者确认的ORR为16%(95%CI,4.5%~36.1%),DCR为84%(95%CI,63.9%~95.5%),中位PFS为5.3个月(95%CI,3.5~6.9个月),中位OS尚未达到。全组患者治疗耐受良好,治疗相关的Ⅲ级或Ⅳ级AR最常见的是白细胞下降(20%)和皮疹(12%),没有与治疗相关的死亡报告。

重组人血管内皮抑制素联合阿法替尼及替吉奥二线治疗晚期肺鳞癌可延长患者PFS且相对安全,值得进一步探究及推广。

     

方向从脾肾论治肝豆状核变性流涎症举隅

流涎是肝豆状核变性常见的非运动症状,近年来在临床上越来越受到重视。文章总结方向主任多年临床经验,从“脾肾”论治肝豆状核变性患者流涎症,方向主任认为肝豆状核变性流涎症病机在于铜毒内聚,脾肾不足,可在通腑排毒的基础上,配伍健脾化湿,温肾摄唾之品,临床疗效显著。     

Protein regulator of cytokinesis 1 overexpression predicts biochemical recurrence in men with prostate cancer

BackgroundProtein regulator of cytokinesis 1 (PRC1) has been reported to be implicated into the completion of cytokinesis and is dys-regulated in a cancer-specific manner. However, it roles in human prostate cancer (PCa) remain unclear. In the current study, we aimed to investigate the expression pattern of PRC1 and its clinical significance in this malignancy.Materials and methodsPRC1 protein expression in human PCa and non-cancerous prostate tissues was detected by immunohistochemistry, which was validated by microarray-based Taylor data at mRNA level. Then, the associations of PRC1 expression with clinicopathological features and clinical outcome of PCa patients were statistically analyzed.ResultsPRC1 expression in PCa tissues, at both mRNA and protein levels, were significantly higher than those in non-cancerous prostate tissues. In addition, the PCa patients with PRC1 overexpression more frequently had high Gleason score, advanced pathological stage, positive metastasis, short overall survival time and positive PSA failure than those with low Gleason score, early pathological stage, negative metastasis, long overall survival time and negative PSA failure (all P < 0.05). Moreover, PRC1 expression was identified as an unfavorable prognostic factor of biochemical recurrence-free survival in PCa patients (P < 0.001).ConclusionThese findings suggest that the aberrant expression of PRC1 may predict biochemical recurrence in men with PCa highlighting its potential as a prognostic marker of this malignancy.     

Exposure-lag-response associations between extreme environmental conditions and primary Sjögren’s syndrome

Clinical Rheumatology - Patients with primary Sjögren’s syndrome (pSS) reportedly believe that their symptoms worsen on extreme weather days due to variations in environmental...