Ezrin在肿瘤组织中的表达及意义

Ezrin是(ezrin/radixin/moesin,ERM)家族的一个成员,是细胞膜和细胞骨架之间重要的联系分子,在癌细胞生长、转移中发挥重要作用。大量研究表明,Ezrin在肿瘤和内皮细胞的相互作用、细胞迁移、细胞黏附、肿瘤的进展和转移过程中起着重要的作用。文中阐述Ezrin对癌细胞生长特性的影响和对癌症患者治疗和预后的意义。     

Reduced information transmission in the internal segment of the globus pallidus of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced rhesus monkey models of Parkinson’s disease

Rhesus monkey models of Parkinson’s disease were induced by injection of N-methy-l-4-phenyl1,2,3,6-tetrahydropyridine. Neural firings were recorded using microelectrodes placed in the internal segment of the globus pallidus. The wavelets and power spectra show gradual power reduction during the disease process along with increased firing rates in the Parkinson’s disease state. Singular values of coefficients decreased considerably during tremor-related activity as well as in the Parkinson’s disease state compared with normal signals, revealing that higher-frequency components weaken when Parkinson’s disease occurs. We speculate that the death of neurons could be reflected by irregular frequency spike trains, and that wavelet packet decomposition can effectively detect the degradation of neurons and the loss of information transmission in the neural circuitry.     

Antitumor activity of a polysaccharide from longan seed on lung cancer cell line A549 in vitro and in vivo

In this study, a water-soluble longan seed polysaccharide (WLSP), with a molecular weight of 57 kDa, was isolated from longan seed. Gas chromatography (GC) analysis showed that WLSP was composed mainly of rhamnose (Rha), mannose (Man), arabinose (Ara), galactose (Gal), and glucose (Glc), with molar ratios of 2.4:1.5:2.3:5.6:6.5. The result in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that WLSP showed a dose-dependent antiproliferative effect on the proliferation of A549 human lung cancer cells, which is consistent with the amount of lactate dehydrogenase (LDH) release from A549 cells. Prompted by this antiproliferative effect, we further examined its antiproliferative mechanism and in vivo anticancer effect. Our results showed that WLSP had the ability to cause cell cycle arrest in G1 phase, activation of caspases 3 and 9, and cleavage of poly[ADP (ribose)] polymerase (PARP) in A549 cells. The result of this in vivo study showed that WLSP could suppress the growth of xenograft A549 tumors and induce apoptosis. Taken together, these results indicate that WLSP exert an anticancer effect in vitro and in vivo and may be useful for the prevention of lung tumorigenesis.     

老年2型糖尿病患者生活质量影响因素研究

目的了解老年2型糖尿病住院患者生活质量的现况及影响因素,为提高患者生活质量提供科学依据。方法应用糖尿病特异性生活质量量表、焦虑自评量表及社会支持评定量表,对西安市3所医院190例老年2型糖尿病患者进行横断面研究。数据分析使用SPSS 18.0进行一般性统计描述,两独立样本t检验或方差分析以及多元逐步回归分析。结果老年2型糖尿病住院患者生活质量总分为(58.56±11.81)分,生理因素(25.97±7.08)分,心理精神因素(20.00±4.65)分,社会关系(6.84±2.09)分,治疗维度(5.75±1.67)分。单因素分析显示,收入水平、并发症数、体育锻炼、低血糖发生次数、照护需求、睡眠质量、睡眠时间、焦虑对生活质量的影响有统计学意义(P0.05);多元逐步回归分析显示:低血糖发生次数、焦虑、照护需求、并发症数量、个人月均收入最终进入回归方程。结论频发低血糖、焦虑、需求照护、并发症较多而收入低的老年2型糖尿病患者生活质量较差。提示在护理工作中,对容易发生低血糖、内心焦虑、并发症较多、需要照护的老年患者应给予更多关怀,督促患者用药后及时进餐以防发生低血糖,同时加强健康教育,使患者认识到糖尿病并发症的预防和治疗具有重要意义,最终提高其生活质量。     

Efficacy and Safety of Niraparib as Maintenance Treatment in Patients With Extensive-Stage SCLC After First-Line Chemotherapy: A Randomized,Double-Blind,Phase 3 Study

IntroductionZL-2306-005 is a randomized, double-blind, multicenter phase 3 study evaluating the efficacy and safety of niraparib, a poly(adenosine diphosphate-ribose) polymerase inhibitor, as first-line maintenance therapy in Chinese patients with platinum-responsive, extensive-stage SCLC (ES-SCLC).MethodsPatients with complete response (CR) or partial response (PR) to standardized, platinum-based first-line chemotherapy were randomized 2:1 to receive niraparib or placebo (300 mg [baseline body weight ≥ 77 kg, platelet count ≥ 150,000/μL] or 200 mg) once daily until progression or unacceptable toxicity. Primary end points were progression-free survival (PFS) (blinded independent central review) and overall survival (sample size planned: 591 patients). Secondary end points included investigator-evaluated PFS and safety.ResultsZL-2306-005 was terminated early owing to ES-SCLC treatment landscape changes (data cutoff: March 20, 2020). During July 2018–February 2020, a total of 185 of 272 patients screened were randomized (niraparib: n = 125 [CR = 1, PR = 124]; placebo: n = 60 [CR = 1, PR = 59]). Median (95% confidence interval [CI]) PFS (blinded independent central review) was 1.54 months (1.41–2.69, niraparib) and 1.36 months (1.31–1.48, placebo); hazard ratio (HR) = 0.66 (95% CI: 0.46–0.95, p = 0.0242). Median overall survival was 9.92 months (9.33–13.54, niraparib) and 11.43 months (9.53–not estimable, placebo); HR = 1.03 (95% CI: 0.62–1.73, p = 0.9052). Median investigator-evaluated PFS was 1.48 months (1.41–2.56, niraparib) and 1.41 months (1.31–2.00, placebo); HR = 0.88 (95% CI: 0.61–1.26; p = 0.4653). Grade greater than or equal to 3 adverse events occurred in 34.4% (niraparib) and 25.0% (placebo) of patients.ConclusionsZL-2306-005 did not reach primary end points. Nevertheless, niraparib as maintenance therapy modestly improved PFS in patients with platinum-responsive ES-SCLC, with acceptable tolerability profile and no new safety signal.     

基于KANO模型的惊厥患儿家属关怀护理服务需求属性研究

目的探讨惊厥患儿家属关怀护理服务需求属性。方法设计KANO模型的惊厥患儿家属关怀护理服务需求调查问卷,对270例惊厥患儿家属进行调查,通过KANO模型质量属性分类方法将患儿家属关怀护理服务内容进行归类。结果惊厥患儿家属关怀护理服务需求中,必备需求项目11项、期望需求项目7项、魅力需求项目5项、无差异需求项目2项;满意度评价为0.13~0.84,重要度评价为0.19~0.82。结论KANO模型能够有效分析惊厥患儿家属关怀护理服务需求属性、满意度和重要度,值得临床推广应用。     

Genomic and Transcriptomic Characterization of Natural Killer T Cell Lymphoma

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NSCLC患者血清中HSP90α的表达及其临床意义

目的:探讨热休克蛋白90α（HSP90α）在非小细胞肺癌（NSCLC）患者血清中的表达水平及其临床意义。方法:选取2015年10月至2016年10月期间，在第四军医大学唐都医院胸外科确诊为NSCLC的患者100例为肺癌组。选取同期在我院进行体检的健康成年人40例为对照组。检测并比较两组血清HSP90α表达水平差异；比较不同肿瘤分期(TNM分期)患者的血清HSP90α水平差异；探讨不同TNM分期与血清HSP90α表达水平之间的关系。培养NSCLC A549细胞，检测其分泌至胞外的HSP90α蛋白；使用HSP90α抑制剂处理A549细胞，MTT法检测细胞增殖能力的变化。结果:肺癌组与对照组患者血清HSP90α表达水平比较，差异有统计学意义（P＜0.001）；不同TNM分期患者血清HSP90α表达水平比较，差异有统计学意义（P<0.001）；Spearman秩相关分析显示，TNM分期与血清HSP90α水平呈正相关(r=0.95，P＜0.05)。体外细胞实验证实A549细胞能分泌HSP90α至细胞外，使用HSP90α抑制剂处理后细胞增殖能力明显下降。结论:NSCLC患者血清中HSP90α表达水平较正常人升高，并且与TNM分期呈正相关。血清HSP90α由肺癌细胞分泌入血，检测其水平对肺癌的诊断与治疗有一定的临床参考价值。