单侧下颌骨缺损重建术后下颌骨形态及髁突位置的三维定量分析

目的：分析单侧下颌骨缺损重建术后下颌骨形态及髁突位置变化,为下一步建立下颌骨定量分析方法提供参考。方法：下颌骨缺损腓骨重建患者术前及术后均行锥形束CT检查,上颌骨定点匹配重叠后定量分析术前与术后下颌骨形态、髁突位置的变化。结果：单侧下颌骨缺损重建术后健侧下颌骨形态及髁突位置变化无统计学意义（P > 0.05）;对于骨缺损不累及髁突的患者,重建术后患侧下颌骨形态及髁突位置变化无统计学意义（P > 0.05）;对于骨缺损累及髁突的患者,重建术后患侧下颌骨的形态变化无统计学意义（P > 0.05）,而髁突位置表现为向外向下移位,差异具有统计学意义（P < 0.05）。结论：单侧下颌骨缺损腓骨重建术可以健侧下颌骨作为匹配区域分析患侧下颌骨形态及位置变化。本研究结果可为建立下颌骨定量分析方法提供参考和依据。     

射频温控热凝术治疗原发性三叉神经痛648例临床分析

目的:总结射频温控热凝术治疗648例原发性三叉神经痛患者的疗效和体会,探讨射频温控热凝术的方法、技巧和手术并发症的处理。方法:对2001年7月—2011年3月在我院分别采用眶下孔入路法、侧入路法、前入路法和其他入路进行射频治疗的648例原发性三叉神经痛患者临床疗效进行分析。结果:648例原发性三叉神经痛患者接受射频治疗,首次止痛率分别为眶下孔法98.3%、侧路法91.0%、前路法95.5%,首次射频治疗总有效率为96.0%,经6个月～2 a随访到的395例患者中,1年内复发率为9.6%,2年内复发率为20.5%,经再次射频治疗或手术治疗均获得完全止痛。结论:射频治疗原发性三叉神经痛疗效可靠,操作简便、适应证广、并发症较少,CT定位能提高穿刺精确度,减少并发症。     

牙周支持治疗阶段患者依从性影响因素分析及对策

目的:探讨慢性牙周炎患者牙周支持治疗阶段依从性影响因素及其对策.方法:自行制订调查问卷,对已完成牙周非手术基础治疗,且已进入牙周支持治疗阶段(SPT)的慢性牙周炎患者,2a后通过门诊随访和电话回访获取资料,进行分析.结果:共回收有效问卷101份,相关因素分析显示,疾病知晓度、医疗服务质量、年龄、文化程度、经济状况等多种因素可影响依从性.结论:影响慢性牙周炎患者依从性的因素较多,提高患者依从性的关键在于有针对性地对患者进行口腔健康教育、定期随访和改善医疗服务质量.     

成品桩树脂核技术在后牙残冠修复中的应用

目的:探讨采用钛合金成品桩结合树脂核技术修复后牙残冠的临床效果.方法:选择经过完善根管治疗的21例患者、26颗残冠进行根管预备,采用现代黏结技术将成品钛合金桩和流动树脂核材料粘固在一起,进行冠修复.结果:经过10年临床观察,21例患者中仅有4例患者的4颗牙修复失败,修复成功率达85％.X线片显示,经桩核冠修复后的残冠,其根尖周牙槽骨的硬骨板密度增强.结论:规范的根管治疗和有效的桩核加强可以恢复残冠的功能,有效保留患牙.     

应用双向电泳-质谱技术筛选羟氯喹治疗口腔扁平苔藓前后的血清差异蛋白

目的:建立羟氯喹治疗口腔扁平苔藓前后的血清双向电泳图谱,比较血清中差异表达的蛋白质,探讨羟氯喹治疗口腔扁平苔藓的可能作用机制。方法:收集羟氯喹治疗口腔扁平苔藓前后的血清,提取相应蛋白,采用双向凝胶电泳和基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)分析鉴定表达差异量较大的蛋白质点,确定所分析的蛋白质类型和功能。采用SAS 9.12软件包对数据进行统计学分析。结果:纤溶酶原前体、载脂蛋白A-的前体、补体C4A/C4B、C2的前体、VitD结合蛋白和假设蛋白在羟氯喹治疗口腔扁平苔藓前后的血清中存在差异表达,差异有显著性。结论:纤溶酶原前体、载脂蛋白A-的前体、补体C4A/C4B、C2的前体、VitD结合蛋白和假设蛋白在羟氯喹治疗口腔扁平苔藓前后的血清中存在差异表达,但结果有待进一步验证。     

Controlling stem cell-mediated bone regeneration through tailored mechanical properties of collagen scaffolds

Mechanical properties of the extracellular matrix (ECM) play an essential role in cell fate determination. To study the role of mechanical properties of ECM in stem cell-mediated bone regeneration, we used a 3D in vivo ossicle model that recapitulates endochondral bone formation. Three-dimensional gelatin scaffolds with distinct stiffness were developed using 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) mediated zero-length crosslinking. The mechanical strength of the scaffolds was significantly increased by EDC treatment, while the microstructure of the scaffold was preserved. Cell behavior on the scaffolds with different mechanical properties was evaluated in vitro and in vivo. EDC-treated scaffolds promoted early chondrogenic differentiation, while it promoted both chondrogenic and osteogenic differentiation at later time points. Both micro-computed tomography and histologic data demonstrated that EDC-treatment significantly increased trabecular bone formation by transplanted cells transduced with AdBMP. Moreover, significantly increased chondrogenesis was observed in the EDC-treated scaffolds. Based on both in vitro and in vivo data, we conclude that the high mechanical strength of 3D scaffolds promoted stem cell mediated bone regeneration by promoting endochondral ossification. These data suggest a new method for harnessing stem cells for bone regeneration in vivo by tailoring the mechanical properties of 3D scaffolds.     

Natural mannosylation of HIV-1 gp120 imposes no immunoregulatory effects in primary human plasmacytoid dendritic cells

Plasmacytoid dendritic cells (pDCs) play a vital role in activation of anti-HIV-1 immunity, and suppression of pDCs might mitigate immune responses against HIV-1. HIV-1 gp120 high-mannose has been attributed immunosuppressive roles in human myeloid DCs, but no receptors for high-mannose have so far been reported on human pDCs. Here we show that upon activation with HIV-1 or by a synthetic compound triggering the same receptor in human pDCs as single-stranded RNA, human pDCs upregulate the mannose receptor (MR, CD206). To examine the functional outcome of this upregulation, inactivated intact or viable HIV-1 particles with various degrees of mannosylation were cultured with pDCs. Activation of pDCs was determined by assaying secretion of IFN-alpha, viability, and upregulation of several pDC-activation markers: CD40, CD86, HLA-DR, CCR7, and PD-L1. The level of activation negatively correlated with degree of mannosylation, however, subsequent reduction in the original mannosylation level had no effect on the pDC phenotype. Furthermore, two of the infectious HIV-1 strains induced profound necrosis in pDCs, also in a mannose-independent manner. We therefore conclude that natural mannosylation of HIV-1 is not involved in HIV-1-mediated immune suppression of pDCs.     

基于CHARLS数据中老年人口腔卫生服务利用现状及影响因素分析

目的 调查中国中老年人口腔卫生服务利用现状,并分析其影响因素,为提高居民口腔卫生服务利用率提供指导.方法 选取中国健康与养老追踪调查(CHARLS)2015年数据,对中国≥45岁20837名中老年人的口腔卫生服务利用现状进行分析,采用logit回归和tobit回归模型探究影响口腔卫生服务利用的因素.结果 20 837名...     

不同浓度牡蛎蛋白肽对大鼠骨髓间充质干细胞增殖和分化能力的影响

目的:探讨不同浓度牡蛎蛋白肽对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的影响.方法:选择具备稳定传代能力的第3代大鼠BMSCs,采用MTT试剂盒,检测细胞在0、10、20、50 mg/L不同牡蛎蛋白肽浓度干预培养24 h、3 d、7 d后的细胞活力.然...