白及在肿瘤治疗中的应用

白及及其提取物用于肝癌栓塞的治疗,化疗的呕吐治疗,食管肿瘤的支架植入、食管、贲门癌术后吻合口瘘的治疗,子宫肌瘤的栓塞治疗,均取得了显著疗效。白及用于多种肿瘤的治疗前景广阔,值得深入研究、发掘与推广,具有可观的社会与经济价值。     

冠状动脉导丝联合球囊下经导管介入封堵卵圆孔未闭1例

卵圆孔未闭(PFO)是目前成人中最为常见的先天性心脏异常之一。近年来的许多研究表明,PFO与不明原因卒中存在着密切的联系,封闭卒中高危人群开放的卵圆孔,有望降低卒中发生率。另外,也发现PFO与减压病、偏头痛、晕厥、短暂性脑缺血发作、冠状动脉非阻塞性心肌梗死等多种病症的发病有关,闭合卵圆孔可能有益于上述患者。本文报道1例发作性晕厥的患者,经胸超声心动图声学造影(cTTE)提示PFO可能,完善经食管超声心动图+cTEE明确PFO存在,故考虑PFO相关心原性晕厥可能性大,遂行冠状动脉导丝联合球囊下经导管介入封堵术。术后半年患者无晕厥再发。     

胰岛素泵快速控制新诊断2型糖尿病患者高血糖状态对胰岛β细胞功能的影响

目的了解胰岛素泵对胰岛β细胞功能的影响。方法对新诊断51例2猁糖尿病（TgDM）患者FBG≥15mmol／L者，分别行胰岛素强化治疗和口服降糖药治疗，观察其血清C肽浓度的变化。结果强化组治疗4天血糖达标后血清C肽曲线下面积无明显变化，继续皮下注射胰岛素治疗半年后明显升高（P〈0．001）。口服降糖药组亦有改善（P〈0．01）。但半年后两组结果显示：应用胰岛素组优于口服降糖药组（P〈O．001）。结论胰岛素泉数日快速控制严重高血糖不能消除高血糖毒性对胰岛β细胞功能的抑制，对新诊断的FBG≥14mmol／L的T2DM患者胰岛素治疗应废持续数月以期改善胰岛功能。     

Adenovirus expressing p27kip1 suppresses growth of established esophageal carcinoma xenograffs

AIM: To investigate the growth suppression of adenovirus expressing p27kip1 on established esophageal tumors in nude mice.METHODS: Esophageal carcinoma xenografts in nude mice were established by tumor tissue mass transplantation. The successfully constructed recombinant adenoviral vectors carrying p27kip1 gene (Adp27kip1) were directly injected into the esophageal tumors in nude mice. Compared to control group, the growth curve of tumor was drawn and the growth inhibition rate of tumor was calculated. The histology of tumors was examined by hematoxylin and eosin (H&E) staining. The expression of p27kip1 and survivin was detected in tumors by immunohistochemical technique.RESULTS: The growth of tumors in gene therapy group with Ad-p27kip1 was obviously suppressed compared to control group (0.42±0.08 g vs 1.17±0.30 g, t=6.39,P＜0.01), the inhibition rate of tumor growth reached 64.1%. Pathological detection showed that the tumors in nude mice were poorly differentiated esophageal squamous carcinoma. In addition, the expression of p27kip1 was increased, while the expression of survivin was decreased in tumors after being transfected with Ad-p27kip1.CONCLUSION: p27kip1 gene therapy mediated by adenovirus vector has a significant inhibitory effect on esophageal carcinoma in vivo. Up-regulated p27kip1expression and down-regulated survivin expression may be its important mechanisms.     

氯强油搽剂对小鼠迟发型变态反应的影响

目的: 研究氯强油搽剂抗小鼠迟发型变态反应的药理作用。方法: 建立氯强油搽剂实验动物高剂量组、中剂量组、低剂量组、莫米松乳膏组、模型对照组、基质对照组、空白对照组,采用二硝基氯苯（DNCB）丙酮溶液诱发小鼠迟发型变态反应的实验动物模型,观察其对迟发型变态反应的影响,用磷酸组织胺测定小鼠致痒阀。结果: 以耳肿胀度为指标,与模型组相比较,氯强油搽剂高、中、低剂量组差异均有统计学意义（P<0.05）;以胸腺指数、脾脏指数为指标,氯强油搽剂高、中剂量组与模型组比较,差异有统计学意义 （P<0.05）,低剂量组与模型组比较,差异具无统计学意义（P>0.05）;与模型组比较,氯强油搽剂高、中、低剂量组磷酸组织胺致痒阀差异均有统计学意义（P<0.05）。结论: 氯强油搽剂局部外用具有良好的抗小鼠迟发型变态反应和止痒作用,适用于临床具有迟发型变态反应特征的皮损治疗。     

A Study on the Inhibitory Effect of Matrine on Gastric Cancer SGC-7901 Cells

The objective of this paper was to investigate the antitumour mechanism of action of matrine by studying its inhibitory effect on gastric cancer SGC-7901 cells. SGC-7901 cells were chosen, and cell-killing capacity of matrine on gastric cancer SGC-7901 cells was determined using MTT assay and single PI staining assay. The results showed that matrine had an inhibitory effect on gastric cancer SGC-7901 cells, which was somewhat dose-dependent. The study concluded that matrine has a significant in-vitro inhibitory effect on SGC-7901 tumour cells, influences cell cycle of SGC-7901 cells, and induces their apoptosis.     

Influence of butyphthalide combined with urinary kallikrein in ACI treatment on neuro-cytokines and vascular endothelial function and its clinical effect

Abstract

Objective: To study the influence of butyphthalide combined with urinary kallikrein in acute cerebral infarction (ACI) treatment on neuro-cytokines and indicators of vascular endothelial function, observe the curative effect and adverse effects, and discuss its safety and feasibility.

Method: 110 ACI patients were chosen as the objects, and classified into observation group (55 cases) and control group (55 cases) according to the method of random number table. Butyphthalide injection combined with urinary kallikrein was adopted for the observation group based on conventional treatment, while cinepazide maleate injection combined with alprostadil injection was applied for the control group based on conventional treatment. The following indicators of both groups were compared before and after treatment: neurotrophic factor (NTF), nerve growth factor (NGF), neuron specific enolase (NSE); content of CXC chemotactic factor ligand 16 (CXCL16), soluble CD ligand (CD40L), Fibulin-5 and high mobility group box B1 (HMGB1); the content of indicators of vascular endothelial function including plasma endothelin ?1 (ET-1) and no therapeutic effects and adverse effects were recorded.

Results: NSE of both groups after treatment decreased obviously, and the content of NTF and NGF increased obviously. NSE content of observation group was lower than that of control group. NTF content and NGF content of observation group were higher than those of control group. The differences had statistical significance (p?<?0.05). The levels of CXCL16, CD40L, Fibulin-5 and HMGB1 declined obviously, compared with pre-treatment, and the levels of observation groups were significantly lower than those of control grip. The differences had statistical significance (p?<?0.05). ET-1 level rose significantly after treatment, and NO level declined obviously after treatment. ET-1 level of observation group was significantly higher than that of control group, and NO level of observation group was significantly lower than that of control group. The difference had statistical significance (p?<?0.05). Clinical effect of observation group was significantly higher than that of control group. The difference had statistical significance (p?<?0.05). The comparison difference of both groups in the occurrence rate of adverse effects had no statistical significance (p?>?0.05).

Conclusion: The application of butyphthalide combined with urinary kallikrein in ACI treatment can effectively inhibit secretion and release of neuro-cytokines, and improve patients’ vascular endothelial function, with significant treatment effect and high safety. Therefore, it deserves to be promoted clinically.     

STAT3介导了血小板源性生长因子BB诱导的大鼠血管平滑肌细胞Pim-1表达

 目的： 观察血小板源性生长因子BB(PDGF-BB)是否可以诱导大鼠血管平滑肌细胞（VSMCs）表达Pim-1及Pim-1对VSMCs增殖的影响,探讨STAT3信号分子在这一过程中的作用,为血管重建性疾病（VRD）的研究提供实验依据。方法： 不同浓度PDGF-BB作用不同时间刺激体外培养的VSMCs,用细胞计数法检测增殖;用real-time RT-PCR 检测Pim-1 mRNA表达水平;Western blotting 检测STAT3 的活性变化;用放线菌素D（actinomycin D）、AG490（JAK特异性抑制剂）及siRNA沉默Pim-1和STAT3进行干预。结果： PDGF-BB（20 μg/L）作用VSMCs 24 h,可以诱导细胞增殖,Pim-1沉默抑制了这一过程;正常未经处理的VSMCs Pim-1 mRNA表达量较低,不同浓度PDGF-BB（10 μg/L~50 μg/L ）作用VSMCs 1 h,Pim-1 mRNA表达明显增加,其中以20 μg/L最显著;用PDGF-BB（20 μg/L）作用VSMCs 不同时间（0.5 h~4 h）,可显著上调Pim-1 mRNA表达,以0.5 h最显著。用actinomycin D及AG490预处理后Pim-1 mRNA表达随之降低。PDGF-BB可激活VSMCs中磷酸化STAT3水平,AG490和转染STAT3-siRNA可抑制 STAT3的磷酸化以及相应的Pim-1 mRNA表达。结论： PDGF-BB可通过Pim-1调节VSMCs增殖;STAT3可能参与了PDGF-BB诱导的VSMCs Pim-1表达。     

黄芪多糖通过活化AMPK和促进骨骼肌FAT/CD36转位改善成肌细胞FFAs代谢

 目的：探讨黄芪多糖(Astragalus polysaccharides, APS)对骨骼肌游离脂肪酸（free fatty acids, FFAs）代谢的影响及其机制。方法：培养小鼠C2C12成肌细胞;MTT法检测不同浓度FFAs作用不同时间对细胞活性的影响。根据MTT结果选取FFAs最适浓度和时间处理细胞并用APS干预,采用乙酰辅酶A合成酶-乙酰辅酶A氧化酶法检测APS干预前后培养液FFAs浓度;Western blotting测APS干预前后细胞膜脂肪酸转位酶(FAT/CD36)、总FAT/CD36、磷酸化腺苷酸活化蛋白激酶（phosphorylated AMP-activated protein kinase, p-AMPK）和总AMPK蛋白表达。结果：FFAs对细胞的毒性呈浓度和时间依赖性。与FFAs组比较,FFAs+APS组细胞膜FAT/CD36及p-AMPK蛋白表达增加(P<0.05),而总FAT/CD36及总AMPK蛋白表达无明显差异(P＞0.05),同时培养液FFAs浓度降低,细胞活性增加(P<0.05)。结论：APS可以增加骨骼肌细胞对FFAs的摄取利用,其机制可能与活化AMPK和促进FAT/CD36转位有关。     

Long-term sero-positivity for IgG,sequelae of respiratory symptoms,and abundance of malformed sperms in a patient recovered from severe COVID-19

European Journal of Clinical Microbiology & Infectious Diseases - Patients with severe coronavirus disease in 2019 (COVID-19 pneumonia) may have many sequelae, which seriously affect their...