针灸适应症的研究现状和发展趋势

To discuss the dominant diseases of acupuncture and moxibustion and their trend in development. At present, the concepts of acupuncture and moxibustion disease spectrum include efficacy-based graded disease spectrum of acupuncture and moxibustion and evidence-based graded disease spectrum of acupuncture and moxibustion. Hence, the ranges of dominant diseases are various in terms of the different concepts. Regarding the efficacy-based graded disease spectrum, 81 dominant diseases are included. In accordance with the principle of disease spectrum of evidence-based acupuncture and moxibustion, the articles of systematic reviews and meta-analysis of acupuncture and moxibustion published in Chinese medical journals were retrieved, and thus 68 dominant diseases of acupuncture and moxibustion were collected. In view of the retrieval result and the effect characteristics of acupuncture and moxibustion, the authors believe that the following 4 aspects should be involved in the development of the dominant diseases of acupuncture and moxibustion, named (1) painful diseases, (2) neurological disorders, (3) mental, cognitive and affective diseases and (4) immune-endocrine disorders.     

颈胸上段食管癌根治性调强放疗±化疗后无复发生存初步分析

目的:探讨接受根治性调强放疗±化疗颈、胸上段食管癌无复发生存(RFS)及影响因素。方法:回顾性分析河北医科大学第四医院2011—2015年间168例颈、胸上段食管癌患者资料。 Kaplan- Meier法计算RFS, Cox模型多因素预后分析。 Logistics模型复发...     

核因子-kB在早期流产患者绒毛组织中的表达

目的观察核因子-kB(NF-kB)在早期流产患者绒毛组织中的表达,从而探讨其与早期流产发病之间的关系。方法通过免疫组织化学方法检测早期流产患者绒毛组织中的NF-kB的表达情况。结果早期流产患者绒毛组织中NF-kB呈高表达。结论早期流产患者中NF-kB的激活参与了早期流产的发病机制。     

胃癌组织中Livin与caspase-9、p53关系的研究进展

胃癌的发生是一个多基因、多步骤、多阶段的复杂过程。Livin在胃癌组织中的表达与caspase-9的表达呈负相关,而与p53呈正相关,提示抑癌基因p53的突变和caspases-9蛋白抑制其介导级联激活反应引起细胞凋亡作用。干扰Livin在胃癌组织中上调或下调机制,影响Livin抗细胞凋亡作用和机制,可能在胃癌的发生和发展中起着重要作用。因此,研究Livin与caspase-9、p53之间的关系,为肿瘤的早期诊断、基因治疗和细胞治疗提供了新的靶点,为胃癌的检测和治疗提供科学依据。     

血管内皮生长因子反义RNA对裸鼠食管癌细胞增殖和凋亡的影响意义

目的：研究血管内皮生长因子（VEGF）反义RNA对裸鼠食管癌移植瘤细胞增殖和凋亡的影响。方法：将转染VEGF反义eDNA和空载体peDNA3．1的食管癌细胞株TE-1分别接种在裸鼠体内；应用免疫组化法、RT-PCR法检测移植瘤组织中VEGF蛋白和mRNA表达情况；流式细胞仪和透射电镜检测肿瘤细胞增殖及凋亡情况。结果：反义组肿瘤组织中VEGF蛋白和mRNA的表达降低，肿瘤细胞的细胞器发生扩张和肿胀、核染色质边集、凝集成块等凋亡形态学改变；Go／G，期细胞明显增多，S期细胞明显减少，细胞增殖指数降低；对照组和空载体组细胞凋亡较少。结论：VEGF反义RNA能抑制裸鼠食管癌细胞的增殖，诱导细胞凋亡，为食管癌基因治疗基础研究提供依据。     

心塞通口服液治疗急性心肌梗塞疗效观察

目的：探讨心塞痛口服液治疗急性心肌梗塞的临床疗效。方法：将75例入选病人随机分为治疗组（A组）45例,对照组（B组）30例。两组病人均给予尿激酶溶栓,A组口服心塞痛口服液,B组口服西药阿司匹林,疗程均为2周。分别观察其血流变,QRS记分,以及梗死面积变化。结果：A组血流变,QRS记分,以及梗死面积变化的改善均明显优于B组（P〈0.05或P〈0.01）。结论：心塞痛口服液对于急性心肌梗塞具有较好的治疗作用。     

血清肝细胞生长因子测定对内科急性肾损伤患者的临床意义

目的：探讨血清肝细胞生长因子（HGF）浓度与急性肾损伤（AKI）的关系。方法：用ELISA法测定AKI患者血清HGF浓度，分析HGF与eGFR、脏器损伤数目、近期预后的关系。结果：HGF浓度：AKI各组均高于正常对照组，并随脏器损伤数目增多而升高（P=0．000）；与eGFR呈负相关（P〈0．05）。结论：HGF可以反映AKI病情的程度。     

单边内翻套入缝合法吻合兔颈动脉的实验研究

目的：探寻一种新的安全、快捷的小血管吻合方法。方法成年新西兰白兔45只，一侧颈动脉行单边内翻套入缝合法为实验组，另一侧行常规对端缝合法为对照组，比较两组的血管吻合时间、血管通畅率，术后1周，2周行血管吻合口的大体观察、组织学及扫描电镜观察。结果血管吻合时间：实验组为（12±2） min，对照组（16±2） min，差异有显著性（P＜0.05），实验组明显低于对照组。术后两组的血管即刻通畅率均为100%，近期通畅率均为98%，两组间差异无显著性（P＞0.05）。组织学及扫描电镜观察示两组血管吻合口愈合过程及时间相似。结论单边内翻套入缝合法吻合小血管快捷、简单，效果可靠。     

Zerumbone induces gastric cancer cells apoptosis: Involving cyclophilin A

Gastric cancer is one of the leading causes for cancer death. There is an urgent need to develop new therapeutic approaches targeting metastatic gastric cancer. It has been reported that zerumbone has the anti-cancer effects in various malignant cells. However, the effect and the mechanism of zerumbone on melanoma cells is still largely unknown. In the study, we determined the actions of zerumbone on the human gastric cancer cell line SGC-7901.We also observed the mechanism by which zerumbone induced gastric cancer cell apoptosis. Our data indicated that zerumbone significantly inhibited the growth of human gastric cancer cells in a dose-dependent manner and apoptosis was the main cause of decreased cell viability in zerumbone −treated cells. The treatment with zerumbone downregulated Cyp A and Bcl-2 levels, upregulated Bax levels, and caused Cytochrome c (Cyt-C) to release, activating Caspase-3. In summary, our study suggests that zerumbone mightinduced human gastric cancer cells apoptosis through down-regulating Cyp A and mitochondria-mediated pathways.     

以硬腭粘骨膜瓣做衬里的颞部扩张皮瓣修复下睑全层缺损

目的:总结应用以硬腭粘骨膜瓣做衬里的颞部扩张皮瓣修复全层下睑缺损的临床经验。方法:Ⅰ期手术选用50-70ml容量的扩张器埋置于颞部皮下,注水扩张2~3个月,Ⅱ期切取硬腭粘骨膜植于扩张皮瓣内侧,形成复合皮瓣,Ⅲ期取出扩张器,将预置成活的复合皮瓣修复眼睑缺损,恢复睑缘正常形态。结果:2008-2012年收治6例下睑缺损患者复合皮瓣全部成活,眼睑外观和功能满意。结论:以硬腭粘骨膜瓣做衬里的颞部扩张皮瓣修复全层下睑缺损效果肯定,具有较高的临床价值。