Relationship between plasma ghrelin levels and insulin resistance and blood pressure in octogenarians

The association between fasting plasma ghrelin levels and insulin resistance and blood pressure(BP) in octogenarians was investigated in this study.A total of 487 unrelated octogenarians(including 203 men and 284 women) were enrolled in this cross-sectional study at the Healthy Care Center of Shanghai East Hospital,Tongji University,China,from October 2008 to April 2009.Plasma ghrelin was determined by using the enzyme linked immunosorbent assay(ELISA).Insulin sensitivity was assessed using the homeostasis ...     

医务社工探访在缓解住院患者心理情绪压力中的运用

目的：讨论医务社工探访在缓解住院患者心理情绪压力中的作用和运用。 方法：通过对医院部分住院患者进行探访，掌握患者的心理情绪状况，分析其原因，运用心理学技巧进行适当的干预，并评价其干预效果。 结果：医务社工探访在干预住院患者心理情绪压力方面具有明显效果，住院患者对于医务社工探访存在潜在需求。 结论：应由专业人员运用专业手法对住院患者进行适时的干预，缓解其心理情绪压力；医务社工探访对于缓解住院患者心理情绪压力具有重要意义。     

Mechanical stretch enhances the expression and activity of osteopontin and MMP-2 via the Akt1/AP-1 pathways in VSMC

Hemodynamic forces causing mechanical stretch (MS) of vascular smooth muscle cells (VSMC) play an important role in vascular remodeling, but the underlying mechanism involved is not fully understood. Thus, this study investigated whether osteopontin (OPN) expression in VSMC was induced by MS, and identified the intracellular signaling pathways involved in OPN production. The plasma level of OPN and its expression in aortic tissue were increased in various animal models of hypertension including spontaneous hypertensive rats and hypertensive mice induced by angiotensin II or L-NAME. When aortic VSMC was stimulated with MS, OPN production was increased, which was markedly attenuated in VSMC treated with PI3K/Akt inhibitor as well as in Akt1-depleted cells, but not in Akt2-depleted cells, suggesting a pivotal role of Akt1 isoform in OPN expression in VSMC. In the promoter assay, MS increased OPN promoter activity, which was attenuated when the region harboring AP-1 binding sites was mutated. The MS-enhanced promoter activity and OPN expression were also decreased in cells treated with AP-1 siRNA or inhibitor. Moreover, MS-induced MMP-2 production was attenuated in cells treated with OPN siRNA or anti-OPN antibody as well as in OPN-deficient VSMC cultured from aorta of OPN deficient mice. In in vivo experiments, the expressions of OPN and MMP-2 were increased in the aortic tissues from hypertensive mice, but these increases were markedly attenuated in OPN-deficient mice with hypertension. In conclusion, these results suggested that OPN expression in the hypertensive vasculature was increased via signaling pathways that involve Akt1/AP-1, leading to vascular remodeling by increasing the production of MMP-2.     

几种心血管源性晕厥诊断方法的比较

目前诊断心血管源性晕厥的方式有心电图、动态心电图、心外循环记录仪、植入性心电记录仪、倾斜试验及电生理试验等多种方法,每种方法都具有各自的优缺点。选择合适的诊断方法可以最大程度地为临床工作者提供信息。因此,了解不同的诊断方法之间的关系对于认识某些心血管疾病特别是心律失常所致的晕厥有重要的意义。     

脂肪肝发生与高脂血症等危险因素的相关性调查

本文对125例脂肪肝及相关危险因素进行回顾性分析,拟通过该研究了解脂肪肝的患病情况及相关的危险因素,为制定有针对性的预防治疗策略提供科学依据。     

论医药项目中进度管理的方法和重要性

进度管理可称为项目的时间管理或工期管理。项目计划的管理就是要时刻对每项工作进度进行监督,然后,对那些出现偏差的工作采取必要措施,以保证项目按照原定计划进度执行,使预定目标按时和在预算范围内实现。只要对医药工程的进度管理采取足够的重视,运用合理的控制方法,工程的进度也可以得到良好的编排和控制,使工程早日保质、保量的按期完成,投入使用,产生效益,有利于后续各项工作的顺利开展。     

MTD系统在冠心病诊疗中的影像学表现初探

目的：我们应用自主研发的医用热成像诊断（medical thermo-diagnose,MTD）系统,采集冠心病病例术前热成像资料及冠状动脉旁路术中心肌热成像数据加以分析,以期为冠心病诊疗提供新的方法。方法：同期临床确诊冠心病,多支病变患者164例,于术前行红外热成像数据采集,其中12例常规行冠状动脉旁路移植术（medical thermo-diagnose,CABG）并于术中行红外热成像数据采集。结果：经红外热成像技术,所有研究对象顺利采集到相应心肌温度数据及相应热区分布变化,对比其他临床辅助检查手段证实了其有效性。结论：红外热成像技术作为一种新型医学诊断技术手段,可安全、有效地应用于冠心病诊疗过程并具指导意义。     

变应性鼻炎对小鼠鼻黏膜核因子-κB p65和糖皮质激素受体表达的影响

目的探讨变应性鼻炎（allergic rhinitis,AR）小鼠鼻黏膜核因子-κB（nuclear factor kappat B,NF-κB）p65和糖皮质激素受体（glucocorticoid receptor,GR）的表达,进一步了解NF—κB p65和GR在AR发病机制中的作用。方法清洁级C57BL6／J小鼠20只,随机分为AR组和对照组,每组10只。AR组用10％卵清蛋白（ovalbumin,OVA）200μl腹腔注射,6％OVA鼻腔局部激发建立AR模型。取鼻面骨,石蜡包埋,连续切片,行HE染色。分别运用免疫组化SABC法和SP法染色,观察鼻黏膜中NF-κB p65和GR的表达情况。结果AR组中10只动物均建模成功,鼻部AR典型症状明显;对照组无明显变化。①形态学改变：对照组鼻黏膜无明显炎症改变;AR组黏膜则显示不同程度水肿,黏膜下嗜酸粒细胞（eosinophils,Eos）、淋巴细胞和单核细胞浸润。②黏膜NF—κB p65表达：NF-κB p65主要表达于上皮细胞和腺上皮细胞的胞浆,偶见胞核。AR组中上皮细胞染色平均光密度值（0．40±0．12）强于对照组（0．23±0．05;P〈0．05）。③GR表达：2组中均有GR表达,主要表达于鼻黏膜上皮、腺上皮及血管内皮细胞的胞核或胞质。AR组中GR的平均光密度值（0．20±0．04）低于对照组（0．36±0．11;P〈0．05）。结论 NF-±κB p65在AR黏膜中表达增强,而GR的表达下降,提示两者的失衡在AR的发病中发挥重要作用。     

纤维内镜下网篮套取气管异物18例

目的探讨电视监视纤维内镜下网篮套取气管异物的疗效。方法对18例气管异物患者采用黏膜表麻,电视监视纤维内镜下网篮套取气管异物,并分析异物的种类、异物停留部位及术后疗效。结果18例气管异物经电视监视纤维内镜下网篮套取均成功取出,术后症状明显消失,无并发症发生。结论黏膜表麻,电视监视纤维内镜下网篮套取气管异物,疗效好,手术创伤小,是治疗气管异物的一种安全、有效的方法。