地西他滨联合小剂量HAG方案治疗老年急性髓系白血病患者的临床效果

目的观察地西他滨联合小剂量HAG方案治疗老年急性髓系白血病(AML)的临床效果。方法选取我院2014年8月至2018年8月收治的老年AML患者62例,根据治疗方案的不同将其分为对照组和观察组,各31例。对照组采用小剂量HAG方案化疗,观察组在对照组基础上加用地西他滨。比较两组的治疗效果。结果观察组的治疗总有效率高于对照组(P<0.05)。两组的不良反应总发生率无显著差异(P>0.05)。结论地西他滨联合小剂量HAG方案治疗老年急性髓系白血病的疗效确切,且安全性高。     

MiR-136 contributes to pre-eclampsia through its effects on apoptosis and angiogenesis of mesenchymal stem cells

IntroductionMesenchymal stem cells (MSCs) play an important role in the pathology of preeclampsia (PE). The survival of MSCs and angiogenesis in the maternal-fetal interface are important for a successful pregnancy. MicroRNA-136 (miR-136) is highly expressed in decidua-derived MSCs (MSCs) from PE compared with healthy donors (NC). The role of the MSCs aberrant expressed miR-136 in PE development is still unclear. In the present study, we examined the impact of miR-136 on the survival of MSCs and angiogenesis in the maternal-fetal interface.MethodsMSCs were extracted and transfected with miR-136 mimic and interfering RNAs using lipofectamine-2000. Then cell apoptosis were tested using flow cytometry. HUVEC tube formation ability was tested on Matrigel co-cultured with conditioned MSCs supernatants.ResultsHigh level of miR-136 could suppress cell proliferation and promote apoptosis of MSCs through targeting BCL2. It could also impairs HUVEC capillary formation by suppressing VEGF.DiscussionMiR-136 significantly increase the apoptosis and suppress the proliferation of MSCs. It could also inhibit the capillary formation and trophoblast cell invasion. These data suggest that decidua-derived miR-136 that is increased in PE is a potential causal factor of PE.     

Association between abnormal thalamic metabolites and sleep disturbance in patients with end-stage renal disease

Sleep disturbances are common in end-stage renal disease (ESRD) patients. However, the underlying neuropathological mechanisms are largely unclear. Previous studies have revealed the important role of the thalamus in the potential mechanisms of sleep disorders. We hypothesized that the sleep disturbances in ESRD patients may correspond to metabolic changes of thalamus and the uremic factors may have a vital contribution on these changes. We performed multi-voxel ¹H-MRS of bilateral thalami in 27 ESRD patients who currently receiving hemodialysis treatment and 21 age-matched healthy volunteers. ESRD patients underwent Pittsburgh Sleep Quality Index (PSQI) scale and restless legs syndrome (RLS) rating scale assessment. Laboratory blood tests including serum creatinine, serum urea, cystatin-C, serum parathyroid hormone (PTH), calcium and phosphorus levels, hemoglobin and hematocrit were performed in all ESRD patients close to the time of the MR examination. We found correlations among elevated PTH, higher PSQI score and RLS rating score in ESRD patients. ESRD patients displayed decreased N-acetylaspartate and creatine ratio (NAA/Cr) of thalami compared with controls. There were significantly negative correlation between NAA/Cr and serum PTH level or PSQI score. The metabolic changes of thalami played an important role in the neuropathological mechanisms of lower sleep quality in ESRD patients. Secondary hyperparathyroidism as one of the main uremia-related factors was closely related to abnormal metabolites of the thalamus in patients with ESRD, revealing the crosstalk procedure between renal impairment and brain function.

     

卵巢浆液性癌中COX-2表达与微血管密度的相关性

目的:探讨卵巢浆液性癌组织中环氧化酶2（COX-2）的表达与癌组织中微血管密度（microvessel density,MVD）的相关性。方法:应用免疫组织化学染色SP法检测94例卵巢浆液性癌组织中COX-2和CD34的表达,分析癌组织中COX-2的表达与癌组织中MVD的相关性。结果:卵巢浆液性癌组织中COX-2阳性表达56例,阴性表达38例。卵巢浆液性癌94 例MVD的中位数为34.33,以34.33为分界线,将其分为两组,高MVD组（44例）和低MVD组（50例）。高MVD组,中位生存时间为16个月,3年生存率为54.55%,5年生存率为27.27%,低MVD组中位生存时间为38个月,3年生存率为78.00%,5年生存率为62.00%。两组间比较,预后差异有统计学意义(P＜0.05);COX-2的表达与MVD值呈正相关性（P＜0.05）。结论:卵巢浆液性癌中COX-2的表达与新生血管相关,可促进肿瘤的发生、发展。     

足背静脉、大隐静脉穿刺在深静脉血栓肢体溶栓中的效果比较

目的探讨足背静脉、大隐静脉穿刺在深静脉血栓肢体溶栓中的效果。方法选择我院收治的297例深静脉血栓患者,根据穿刺方法将其分为A组(163例,足背静脉穿刺)和B组(134例,大隐静脉穿刺)。比较两组的治疗效果。结果A组一次穿刺成功率、留置针留置时间、住院时间、住院费用均优于B组(P<0.05)。A组的抗凝溶栓治疗总有效率明显高于B组,并发症总发生率明显低于B组(P<0.05)。结论足背静脉穿刺在深静脉血栓肢体溶栓中的效果显著,一次性穿刺成功率高,并发症少。     

路径护理干预对重症烧伤患者急救期营养状态的影响

目的分析路径护理干预对重症烧伤患者急救期营养状态的影响。方法选取我院2018年4月至2020年4月收治的88例重症烧伤患者作为研究对象,按照护理方法的不同将其分为对照组(n=44)与试验组(n=44)。对照组接受常规护理,试验组在对照组基础上接受路径护理。比较两组干预前、后营养状态、疼痛程度、生活质量及护理满意度。结果干预后,试验组血红蛋白、血清白蛋白水平及总氮平衡明显高于对照组(P<0.05)。干预后,试验组VAS评分明显低于对照组,SF-36评分高于对照组(P<0.05)。试验组护理满意度为95.45%,明显高于对照组的81.82%,差异具有统计学意义(P<0.05)。结论路径护理应用于重症烧伤患者急救期中的效果显著,可有效改善患者的营养状态,值得临床推广应用。     

标准化护理程序对CCU心力衰竭患者病情改善的影响

目的分析标准化护理程序对冠心病监护病房(CCU)心力衰竭患者病情改善的影响。方法选取2018年1月至2020年1月我院CCU收治的300例心力衰竭患者作为研究对象,按照护理方式将其分为观察组(标准化护理程序)和对照组(常规护理),各150例。比较两组的护理效果。结果护理后,两组患者心率、舒张压、收缩压、BNP水平、LVEF及SF-36、HAMD、HAMA、护理质量各项评分均改善,且观察组优于对照组(P<0.05)。结论标准化护理程序对CCU心力衰竭患者病情改善起积极作用,能有效改善其心理状态和生活质量,提高护理质量,值得临床推广和应用。     

优质护理干预对尿毒症血液透析患者依从性、负性情绪的影响

目的分析优质护理干预对尿毒症血液透析患者依从性、负性情绪的影响。方法将我院2019年1月至2020年1月收治的80例尿毒症血液透析患者按入院顺序分为对照组(40例,常规护理)和试验组(40例,优质护理)。比较两组的护理效果。结果护理后,试验组饮食、液体摄入、用药及透析方案依从性评分均高于对照组(P<0.05)。护理后,试验组SAS、SDS、SPBS及MNA评分均优于对照组(P<0.05)。试验组并发症总发生率低于对照组(P<0.05)。结论优质护理干预应用于尿毒症血液透析治疗中可提高患者依从性,明显改善负性情绪及营养状况,减少并发症,值得临床推广应用。     

还原型谷胱甘肽联合酪酸梭菌二联活菌散对新生儿黄疸胆红素水平及T细胞亚群指标的影响

目的探讨还原型谷胱甘肽联合酪酸梭菌二联活菌散对新生儿黄疸胆红素水平及T细胞亚群指标的影响。方法将110例新生儿黄疸患儿随机分为对照组和观察组,各55例。对照组采用还原型谷胱甘肽治疗,观察组采用还原型谷胱甘肽联合酪酸梭菌二联活菌散治疗。比较两组的临床效果。结果观察组的治疗总有效率高于对照组(P<0.05)。治疗后,观察组的血清胆红素水平低于对照组(P<0.05)。治疗后,观察组的CD4~+及CD4~+/CD8~+均高于对照组(P<0.05)。治疗后,观察组的MDA水平低于对照组,SOD水平高于对照组(P<0.05)。结论还原型谷胱甘肽联合酪酸梭菌二联活菌散治疗新生儿黄疸的效果显著,可促进患儿血清胆红素水平降低和免疫功能提升,也能减轻氧化应激反应。     

秦皮滴眼液联合睑板腺挤压器治疗睑板腺功能障碍性干眼的临床效果

目的探讨秦皮滴眼液联合睑板腺挤压器治疗睑板腺功能障碍性干眼的临床效果。方法选取120例睑板腺功能障碍性干眼患者为试验对象,采用单双号随机化法将其分为观察组(60例,秦皮滴眼液+睑板腺挤压器)和对照组(60例,睑板腺挤压器)。比较两组的治疗效果。结果治疗后30 d,观察组的临床症状积分、OSDI、FL、睑板腺分泌物质量、睑板腺分泌能力评分均明显低于对照组(P<0.05)。治疗后30 d,观察组的SⅠt、BUT、泪膜厚度均明显优于对照组(P<0.05)。结论秦皮滴眼液联合睑板腺挤压器治疗睑板腺功能障碍性干眼的临床效果显著。