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排序方式: 共有428条查询结果,搜索用时 31 毫秒
411.
目的:研究降血糖中药复方降糖颗粒提取的最佳工艺条件。方法:以总提取率和黄芪甲苷为指标用高效液相色谱法对复方的提取方式进行正交实验研究。结果:降糖颗粒提取的最佳工艺条件为A2B3C2,即:加8倍水,煎煮3次,每次煎煮2 h,可以获得较高提取效率和黄芪甲苷的含量。结论:通过正交试验,以方中主要活性成分黄芪甲苷提取量及总提取率作为主要考察指标,并综合考虑实际生产,优选最佳提取工艺条件,为该药品的生产提供科学依据。 相似文献
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目的 基于细胞凋亡Fas和TRAIL死亡受体信号通路探讨黄芪注射液对大鼠急性脊髓损伤(SCI)的神经保护作用及机制。方法 40只健康的雄性SD大鼠随机分为假手术组,SCI模型组,黄芪注射液低、中、高剂量(1、2、4 mL·kg-1)组,每组8只。采用改良的重物打击法构建大鼠急性SCI模型;按照BBB评分和Rivlin斜板实验评价大鼠运动功能;苏木精-伊红(Hematoxylin-eosin, HE)染色检测脊髓组织病理变化;尼氏染色法检测脊髓神经元细胞损伤程度;高通量转录组测序分析差异表达基因;qPCR验证基因转录水平的表达量;利用TUNEL试剂盒检测各组脊髓细胞凋亡情况;Western blot检测凋亡通路关键蛋白表达水平。结果 BBB评分和斜板试验结果显示,与假手术组比较,模型组大鼠表现出明显的运动功能障碍;HE染色显示模型组脊髓组织病理病变严重;模型组尼氏染色着色较浅,神经元细胞损伤严重;与假手术组比较,模型组脊髓中4 597个基因差异表达,Fas、TRAIL、Caspase-3、Caspase-8、Caspase-9和Bax蛋白表达显著上调(P<0.... 相似文献
414.
Shuting Meng Longxiao Jing Weiwei Zhang Feng Wang Yefeng Dong Deping Dong 《Journal of clinical laboratory analysis》2022,36(9)
BackgroundAs a chronic systemic autoimmune disease of undetermined etiology, rheumatoid arthritis (RA) has a complex pathogenesis, which involves multiple proteins and cytokines. The 2010 ACR/EULAR classification criteria facilitate early diagnosis of RA with reduced specificity when compared to the 1987 ACR criteria. Hence, it is imperative to identify novel serological inflammatory indicators and targets, in order to explain the complex regulatory network of RA. The present review discusses the associations of various inflammatory factors with RA and its underlying mechanism. Besides, the review also provides a novel insight into the clinical treatment of RA.Materials and MethodsAccording to the PRISMA guidelines, databases like Web of Science, Google‐Scholar, Pubmed and Scopus were systematically searched for articles from January 1, 2018 to January 1, 2022 using The following 2 keywords: "rheumatoid arthritis", "Inflammatory cytokines", "ILs", "serum amyloid protein A", "matrix metalloproteinase 3", "RANKL", "Glucose‐6‐phosphoisomerase", "Anti‐keratin antibody", "1,25‐Dihydroxyvitamin D3".ResultsIndicators like MMPs, ILs, glucose‐6‐phosphate isomerase (GPI), anti‐keratin antibody (AKA) and receptor activator of nuclear factor‐κB ligand (RANKL) are the current hotspots in the efficacy research of RA. The present review suggests that ILs are highly expressed in the serum and synovial tissues of RA patients. By targeted inhibition of ILs with inhibitor application, precise RA treatment can be achieved.ConclusionsBased on these results, it can be concluded that inflammatory factors have certain guiding significance in the diagnosis and efficacy evaluation of RA. However, the mechanisms of interactions among them are rather complex, which deserve further exploration. 相似文献
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《Journal of diabetes and its complications》2023,37(9):108566
Maturity-onset diabetes of the young (MODY) is an inherited form of diabetes resulting from a mutation in a single gene. ABCC8-MODY is caused by mutations in the ABCC8 gene, which encodes sulfonylurea receptor 1 (SUR1), a regulatory component of the ATP-sensitive potassium (KATP) channel found in beta cells. In ABCC8-MODY, mutations in the ABCC8 gene interfere with insulin secretion in response to glucose. Recent evidence suggests that therapy with GLP-1 receptor agonists (GLP-1 RAs) may be beneficial in ABCC8-MODY. This report presents a successful treatment of a 49-year-old woman diagnosed with ABCC8-MODY using the GLP-1 RA semaglutide. The patient, who had been previously receiving insulin therapy, experienced significant improvements in glycemic control and weight loss after transitioning to semaglutide. GLP-1 RAs potentially enhance insulin secretion in ABCC8-MODY by activating multiple signaling pathways involved in insulin secretion. The report highlights the potential of GLP-1 RA therapy as an alternative to sulfonylureas and insulin for individuals with ABCC8-MODY. GLP-1 RAs have previously demonstrated benefits in other forms of MODY. Understanding the molecular mechanisms through which GLP-1 RAs promote insulin secretion, including their effects on KATP channels and activation of PKA and Epac signaling, offers valuable insights into their therapeutic effects. 相似文献
420.
《Transplantation proceedings》2023,55(7):1763-1764