Life satisfaction and its influencing factors for bedridden patients with stroke

ObjectivesThis study aimed to evaluate the life satisfaction of bedridden patients with stroke and explore its relationship with demographic, social, and medical factors.Material and methodsThis multicenter cross-sectional study was conducted in two steps. The Longshi scale was used to select the study population and assess patients’ ability to perform activities of daily living. Subsequently, a multidimensional questionnaire was used to obtain the participants’ information and evaluate their level of life satisfaction. The chi-squared test and binary logistic regression methods were employed to analyze the factors influencing the life satisfaction of bedridden patients with stroke.ResultsA total of 3,639 bedridden patients with stroke were included in this study, of them, only 27.2% reported satisfaction with their current lives. Factors associated with higher life satisfaction include female sex, older age, and primary school education or lower (P<0.05). Patients who had experienced a single stroke episode had chronic diseases, and rated their health as good were more satisfied with their lives than those who did not. The results of the binary logistic regression confirmed that age, education, religion, household income, cohabitation, social participation, number of chronic diseases, self-rated health status, and disability level significantly influenced the life satisfaction of bedridden patients with stroke (P<0.05).ConclusionOur study showed that the overall life satisfaction of bedridden patients with stroke was low, with several factors influencing their life satisfaction. Therefore, effective measures should be implemented to improve life satisfaction and quality of life.     

Appraisal of treatment outcomes in integrative medicine using metabonomics: Taking non-alcoholic fatty liver disease with spleen deficiency syndrome as an example

ObjectiveAppraisal of treatment outcomes in integrative medicine is a challenge due to a gap between the concepts of Western medicine (WM) disease and traditional Chinese medicine (TCM) syndrome. This study presents an approach for the appraisal of integrative medicine that is based on targeted metabolomics. We use non-alcoholic fatty liver disease with spleen deficiency syndrome as a test case.MethodsA patient-reported outcome (PRO) scale was developed based on literature review, Delphi consensus survey, and reliability and validity test, to quantitatively evaluate spleen deficiency syndrome. Then, a metabonomic foundation for the treatment of non-alcoholic fatty liver disease with spleen deficiency syndrome was identified via a longitudinal interventional trial and targeted metabolomics. Finally, an integrated appraisal model was established by identifying metabolites that responded in the treatment of WM disease and TCM syndrome as positive outcomes and using other aspects of the metabonomic foundation as independent variables.ResultsTen symptoms and signs were included in the spleen deficiency PRO scale. The internal reliability, content validity, discriminative validity and structural validity of the scale were all qualified. Based on treatment responses to treatments for WM disease (homeostasis model assessment of insulin resistance) or TCM syndrome (spleen deficiency PRO scale score) from a previous randomized controlled trial, two cohorts comprised of 30 participants each were established for targeted metabolomics detection. Twenty-five metabolites were found to be involved in successful treatment outcomes to both WM and TCM, following quantitative comparison and multivariate analysis. Finally, the model of the integrated appraisal system was exploratively established using binary logistic regression; it included 9 core metabolites and had the prediction probability of 83.3%.ConclusionThis study presented a new and comprehensive research route for integrative appraisal of treatment outcomes for WM disease and TCM syndrome. Critical research techniques used in this research included the development of a TCM syndrome assessment tool, a longitudinal interventional trial with verified TCM treatment, identification of homogeneous metabolites, and statistical modeling.     

Evaluating the Link Between Self-Reported Endometriosis and Female Sexual Dysfunction

BackgroundStudies have found that women with endometriosis have a higher risk of female sexual dysfunction (FSD).AimTo evaluate the relationship between self-reported endometriosis and FSD utilizing validated surveys.MethodsA cross-sectional analysis was conducted among sexually active women aged 18–90 who presented to 3 Mayo Clinic sites from 2015 to 2021. FSD was determined utilizing a combined endpoint of Female Sexual Function Index score ≤ 26.55 and Female Sexual Distress Scale-Revised score ≥ 11. Associations between history of endometriosis and FSD were evaluated by fitting 3 multivariable logistic models and were stratified by menopause status. In the first model, the association was adjusted for age, BMI, race/ethnicity, marital status, and education. The second model adjusted for the variables in Model 1 and hormone therapy, hormonal contraceptive use, self-reported history of abuse within the last year, and co-morbidities including the history of diabetes, heart disease, hypertension, osteoporosis, and stroke. The third model adjusted for the variables in Model 1, Model 2, and anxiety, depression, relationship satisfaction, and SSRI/SNRI use.OutcomesThe outcomes included self-reported endometriosis and female sexual dysfunction determined utilizing a combined endpoint of Female Sexual Function Index score ≤ 26.55 and Female Sexual Distress Scale-Revised score ≥ 11.ResultsOf 7118 patients (mean age 51.3), 92.2% were white, 78.4% were peri- or postmenopausal, 8.7% reported endometriosis history, and 57.2% met the criteria for FSD. Women with endometriosis were more likely to be overweight or obese, be smokers, have had a history of heart disease and osteoporosis, have had anxiety and depressed mood, have had a hysterectomy and bilateral salpingo-oophorectomy, and have used hormone therapy. Compared to those without endometriosis, women with endometriosis were significantly more likely to have FSD only among premenopausal women (74.2% vs 57.4%). Similarly, in multivariable analysis the relationship was only seen for premenopausal women in all 3 models (Model 1: OR 2.74 (95% CI 1.43–5.27); Model 2: OR 2.55 (95% CI 1.30–5.04); Model 3: OR 2.30 (95% CI 1.13–4.68)).Clinical ImplicationsThese findings highlight the opportunity for healthcare practitioners to evaluate sexual function in premenopausal women with endometriosis. For peri and postmenopausal women with endometriosis, the risk of FSD was lower than for premenopausal women with endometriosis.Strengths and LimitationsThis study analyzed the association between endometriosis and FSD in women by menopause status using validated tools that included a measure of distress associated with sexual dysfunction. Limitations include its cross-sectional design which does not allow for determination of the direction of this association.ConclusionThe risk for FSD associated with endometriosis depends on menopause status. Endometriosis increased the odds of FSD only in premenopausal women.Kling JM, Ghaith S, Smith T, et al. Evaluating the Link Between Self-Reported Endometriosis and Female Sexual Dysfunction. J Sex Med 2022;19:1533–1561.     

不同产地白芍药材的指纹图谱研究

目的：建立白芍药材的高效液相色谱和薄层色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法：利用ＨＰＬＣ方法,梯度洗脱,测定了 １０批白芍样品。色谱条件为：ＫｒｏｍａｓｉｌＣ１８色谱柱（２５０ｍｍ×４．６ｍｍ,５μｍ）,流速 １．０ｍＬ·ｍｉｎ－１,柱温为 ２５℃,流动相 Ａ为乙腈;流动相 Ｂ为０．０５％磷酸水,分析时间为９５ｍｉｎ。同时利用 ＴＬＣ方法,建立１０批白芍药材的薄层色谱指纹图谱,展开剂为三氯甲烷 －乙酸乙酯 －甲醇 －甲酸（４０５１００．２）。结果：１０批白芍药材样品 ＨＰＬＣ指纹图谱获得包括芍药苷（９号峰）在内的 １５个共有峰,其中安徽、浙江的白芍饮片的指纹图谱相互之间差异较小。结论：白芍药材的指纹图谱特征性及专属性强,可用于全面控制白芍的质量,确保每批产品的均一性。     

MiR-125b靶向A20/NF-κB信号通路促进胰腺癌细胞增殖机制的研究

[目的]研究miR-125b在胰腺癌组织及细胞系中的表达,探讨其对胰腺癌细胞增殖的影响及作用机制。[方法]QRT-PCR检测miR-125b在60例胰腺癌患者癌组织和癌旁组织中,及3株胰腺癌细胞系和1株正常胰腺导管上皮细胞中的表达;miR-125b敲减慢病毒质粒及阴性对照空载体质粒vector感染SUIT-2细胞,经1.0μg/ml嘌呤霉素筛选稳定敲减miR-125b或vector的SUIT-2细胞,注射到BALB/c裸鼠中,观察抑制miR-125b对胰腺癌细胞体内增殖能力的影响;利用Targetscan 7.2软件及荧光素酶报告基因试验,分析miR-125b对A20基因的靶向作用;SUIT-2细胞转染miR-125b inhibit和scramble后,体外实验检测miR-125b对细胞增殖、肿瘤坏死因子α诱导的蛋白3(tumor necrosis factorα-induced protein 3,TNFAIP3,又称A20)和NF-κB信号通路关键蛋白IKKα/β、IκBα、p65的表达影响。[结果]MiR-125b在胰腺癌的组织中的表达显著高于癌旁组织(0.99±0.21 vs 0.68±0.17,P<0.05);miR-125b在SW1990、SUIT-2、BxPC3胰腺癌细胞系中的表达显著高于在正常胰腺导管上皮细胞HPDE6-C7中的表达(4.81±0.13,8.63±0.27,5.64±0.21 vs1.00±0.05,P均<0.05);SUIT-2_(mi R-125b)的裸鼠成瘤体积显著低于SUIT-2_vector细胞(P<0.05)。TargetScan软件预测和荧光素酶报告基因实验验证A20为miR-125b的靶基因;转染miR-125b inhibit后,A20基因表达显著增加(6.98±0.18 vs 1.00±0.06,P<0.05),细胞增殖能力、NF-κB信号通路关键蛋白IK-Kα/β、IκBα、p65的表达显著下降(P均<0.05)。[结论]MiR-125b可通过靶向调控A20/NF-κB信号通路促进胰腺癌细胞的增殖。     

经鼻超细电子胃镜检查术的护理

目的探讨护理技术在超细经鼻电子胃镜检查术中的运用。方法经鼻进行超细电子胃镜检查,并在术前、术中、术后护理配合。结果 1 228例患者,一次性进鼻送镜成功1 151例,成功率为93.7%。因鼻骨术后11例,鼻甲肥大、鼻腔狭小等58例,害怕经鼻8例,共77例改为经口检查。术后鼻腔轻度出血22例(1.9%),自觉鼻腔疼痛84例(7.2%)。结论超细经鼻电子胃镜检查术,具有术前恐惧感小、术中不适感轻、术中术后并发症少的优势,但操作技术难度、护理配合要求较高。     

Target identification and occupancy measurement of necrosis avid agent rhein using bioorthogonal chemistry-enabling probes

Necrosis is an important biomarker, which only occurs in pathological situations. Tracking of necrosis avid agents is of crucial importance toward understanding their mechanisms. Herein, we developed a modular probe strategy based on bioorthogonal copper-free click chemistry. Structural modification of rhein with transcyclooctene (TCO) led to the identification of rhein-TCO2 as the most active probe with specific necrosis affinity. In a systematic evaluation, the colocalization of rhein-TCO2 in the nucleus (exposed DNA and rRNA) of necrotic cells was observed. This work provides a foundation for the development of target-identified of rhein compounds, and binding to exposed DNA and rRNA may be an important target of rhein compounds in necrotic cells.

Structural modification of rhein with transcyclooctene (TCO) led to the identification of rhein-TCO2 as the most active probe with specific necrosis affinity.     

维生素A辅助治疗小儿肺炎的Meta分析

目的：系统评价维生素A辅助治疗小儿肺炎的疗效。方法：对应用维生素A辅助治疗小儿肺炎的随机对照试验或半随机对照试验进行Meta分析。结果：共纳入11个随机对照试验,Meta分析结果维生素A辅助治疗组总有效率明显高于对照组（OR=7．81,95％CI：1.41～43．23,P〈0．001）;能增加X线胸片阴转率（OR=7．34,95％CI：3．22～16．73,P〈0．001）;发热持续时间比较差异无统计学意义（WMD-0．29,95％CI：-0．58～0．00,P=O．05）;能缩短咳嗽持续时间（WMD=-0．97,95％CI：-1．87～0．06,P〈O．05）;能缩短肺部罗音消失时间（WMD=-0．61,95％CI：-0．88-O．34,P〈0．001）;能缩短患儿住院时间（WMD-0．87,95％CI：-1．61～0．13,P〈0．05）。结论：维生素A辅助治疗小儿肺炎具有较好的疗效。     

三七总皂苷玉米脂蛋白纳米粒对大鼠脑缺血再灌注损伤的药效学研究

对三七总皂苷(Panax notoginseng saponins,PNS)与卵磷脂、β-谷甾醇、玉米醇溶蛋白共组装制备的三七总皂苷玉米脂蛋白纳米粒(PNS-lipid-zein nanoparticles,PLZ-NPs)进行了体外细胞实验,以及大鼠脑缺血再灌注损伤的药效学研究.采用反溶剂沉淀法制备了PLZ-NPs,...     

Gene MTB/RIF、TB-IGRA检测与传统组织病理学检查在脊柱结核诊断中的应用价值

目的:评估传统组织病理学(简称"病理")检查与Gene MTB/RIF(简称"Xpert")、γ干扰素释放试验(TB-IGRA)在脊柱结核诊断中的应用价值.方法:回顾性分析2017年11月～2020年6月在我院经临床诊断为"脊柱结核"的131例患者资料,男79例,女52例;年龄18～90岁(50±18.0岁);颈椎7例...