肿瘤坏死因子对肝细胞的影响

通过体外实验发现，在５００Ｕ／ｍｌ和２０００Ｕ／ｍｌ浓度的肿瘤坏死因子（ＴＮＦ）刺激下，肝细胞（ＨＣ）的ＤＮＡ复制增强，但是８０００Ｕ／ｍｌ浓度的ＴＮＦ抑制ＨＣ内ＤＮＡ的复制，并且细胞内酶逸出增加，脂多糖（ＬＰＳ）自身对ＨＣ的ＤＮＡ代谢没有影响，将ＬＰＳ加入到ＨＣ和非肝实质细胞的混合培养液中，则细胞的ＤＮＡ复制受阻，结果提示，高浓度ＴＮＦ会导致ＨＣ的损伤，枯否细胞对ＨＣ的损伤是由ＴＮＦ介导的。     

Liver transplantation from non–heart beating donors in rats: Influence of viscosity and temperature of initial flushing solutions on graft function

BACKGROUND: We evaluated the effect of warm (37 degrees C) versus cold (4 degrees C) solutions as the initial flush for liver preservation from non-heart beating donors in rats. METHODS: An initial flush was performed just before donor hepatectomy with cold or warm University of Wisconsin solution (UW), UW without hydroxyethyl starch, sodium lactobionate sucrose solution, or lactated Ringer's solution as the control group. A separate group also used as control received no initial flushing. Liver transplantation was performed, and the graft function was determined by survival and assessment of enzyme release. The viscosity of each solution and the vascular resistance of the graft were measured. RESULTS: The 7-day survival rate was 83% and 100% in the warm and cold sodium lactobionate sucrose solution groups and 60% and 50% in the warm and cold lactated Ringer's solution groups, respectively. In the no-initial-flush group, rats did not survive. The 7-day survival rate was 67% and 0% in the warm and cold UW groups, respectively. Eliminating the hydroxyethyl starch from the cold UW improved the survival to 67%. Serum alanine aminotransferase levels 1 day after transplantation in the no-initial-flush and the cold UW groups were significantly higher than those of the remaining groups. At 4 degrees C the viscosity was higher in the UW (86.2 cp) compared to hydroxyethyl starch-free UW solution (30.9 cp), lactated Ringer's solution (24.5 cp), and sodium lactobionate sucrose solution (32.7 cp). The viscosity of UW at 37 degrees C was 34.7 cp. Vascular resistance correlated well with the viscosity. Livers flushed with solutions with a low viscosity showed lower vascular resistance than those flushed with cold UW and led to better survival. CONCLUSIONS: These data suggest that the viscosity of the initial flushing solution may play an important role in determining the outcome of organ procurement from non- heart beating donors. (Liver Transpl Surg 1997 Jan;3(1):39-45)     

CDCA7通过与MCM3相互作用介导胰腺癌细胞增殖、侵袭及迁移

目的:探讨细胞分裂周期相关蛋白7(cell division cycle-associated protein 7,CDCA7)对人胰腺癌细胞增殖、侵袭及迁移能力的影响,及其可能的作用机制.方法:利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达谱数据动态分析(Gene Expre...     

六味五灵片治疗抗结核药物所致肝损伤疗效观察

目的 探讨六味五灵片治疗抗结核药物所致肝损伤的临床疗效。方法 将72例抗结核药物所致肝损伤患者随机分为治疗组（38例）和对照组（34例）,两组患者应用抗结核药物的同时,治疗组加用六味五灵片,对照组加用护肝片。两组患者疗程均为4周,且治疗结束后随访2周。比较两组患者肝功能改变。结果 治疗组在治疗及随访结束后ALT、AST、TBil复常率与对照组比较,差异有显著统计学意义（P ＜ 0.01）。结论 六味五灵片可以改善受损肝脏功能,减轻肝内炎性反应,治疗抗结核药物所致肝损伤效果显著。     

Combined radiochemotherapy in patients with locally advanced pancreatic cancer: A meta-analysis

AIM:To compare the long-term clinical efficacy of chemotherapy plus radiotherapy(CRT)with that of radiotherapy alone(RT)or chemotherapy alone(CT)for locally advanced pancreatic carcinoma(LAPC).METHODS:Using manual and computer-aided methods,we searched the data through the databases,including PubMed/EmBase/CNKI/CQVIP/China Journals Full Text Database and websites and proceedings of major annual meetings such as ASCO and CSCO.The methodological quality of the included studies was assessed using the Jadad scoring system.Both English and Chinese publications were searched.We collected data from controlled clinical trials on CRT vs RT or CT for LAPC,and conducted a meta-analysis of 15 included studies.Meta-analysis was performed using RevMan4.2Software according to the method recommended by Cochrane Collaboration.RESULTS:Fifteen eligible randomized controlled trials including a total of 1128 patients were screened.Jadad score was 2 in only one article,and 3-4 in the remaining 14 studies.The meta-analysis showed that CRT was superior in the 6-and 12-mo survivals to the RT alone group or CT alone group(P=0.0001 and P=0.02,respectively),whereas the 18-mo survival showed no significant difference(P=0.23).Subgroup analysis showed that the 6-,12-,and 18-mo survivals were not significantly different between the CRT group and CT group(P=0.07,P=0.23,and P=0.91,respectively).Notably,the CRT group had significantly better 6-,12-,and 18-mo survivals than the RT group(all P<0.01).CRT group had significantly more grade 3-4 treatmentrelated hematologic and non-hematologic toxicities than the CT group or RT group(all P<0.01).CONCLUSION:Compared with CT or RT,CRT can benefit the long-term survival of LAPC patients,although it may also increase treatment-related toxicities.     

乙肝核心抗体阳性供肝在肝移植中的应用

背景：现已经证实使用anti-HBc(+)供肝会使移植后乙肝复发的风险,但anti-HBc(+)供肝的应用明显缓解了供肝的相对匮乏。 目的：分析应用anti-HBc(+)供肝移植后乙肝复发风险及有效的预防措施。 方法：应用计算机检PubMed数据库中1994-01/2009-12关于anti-HBc(+)供肝文章,在标题和摘要中以“Hepatitis B core antibody; donor;liver transplantation”为检索词进行检索。选择与anti-HBc供肝相关文章。初检得到109篇文献,根据纳入标准选择48篇文章进行综述。 结果与结论：HBsAg(+)患者接受anti-HBc(+)供肝移植术后乙肝复发率为11%,生存率为67%~100%,与HBsAg(+)受者接受anti-HBc(-)供肝相似。HBsAg(-)受者接受anti-HBc(+)供肝总体感染率为19%,其中未感染过乙肝受者移植术后乙肝感染率为48%,感染过乙肝受者后感染率为15%。未感染乙肝与感染过乙肝受者移植后采取有效预防措施后感染率分别为3%,12%。采用HBIG、拉米夫定、联合用药的移植后感染率分别为19%,2.6%,2.8%。提示,采用anti-HBc(+)供肝做为供体是安全的,尤其是用在HBsAg(+)、anti-HBc(+)、anti-HBs(+)受者。而HBsAg(-)受者移植后接受拉米夫定可以有效复发乙肝感染。     

还原型谷胱甘肽治疗慢性乙型肝炎142例临床观察

目的：观察还原型谷胱甘肽治疗慢性乙型肝炎的临床疗效。方法：通过对照观察还原型谷胱甘肽治疗慢性乙型肝炎的临床资料,观察患者恢复情况,分析治疗结果。结果：治疗组改善临床症状显著高于对照组,差异有统计学意义（P〈0.01）。结论：还原型谷胱甘肽对慢性乙型肝炎有较好的治疗作用。     

Common traditional Chinese medicine therapies for diarrhea-predominant irritable bowel syndrome: An overview of systematic reviewsOA

Objective: To critically evaluate and summarize the methodological quality of systematic reviews(SRs)and present objective and important outcomes on the effectiveness of traditional Chinese medicine(TCM) therapies, including Chinese herbal medicine(CHM), acupuncture, and moxibustion, for diarrheapredominant irritable bowel syndrome(IBS-D).Methods: We conducted a comprehensive literature search for SRs in 7 databases until April 16, 2022.Two reviewers independently extracted data and assessed the...