急性重型颅脑损伤合并休克25例救治体会

目的：总结重型颅脑损伤合并休克患者的救治及临床特点，分析其休克发生的原因及机理。方法：对我院收治的25例资料完整的重型颅脑损伤合并休克患者进行回顾性分析，对患者严密监护，及时进行手术、抗休克及其他综合治疗。结果：本组25例中15例成活，10例死亡，死亡率为40％。结论：早期迅速查明休克原因，正确早期治疗，可明显改善预后。     

Identification of cancer-related gene network in hepatocellular carcinoma by combined bioinformatic approach and experimental validation

HCC (hepatocellular carcinoma) is a highly aggressive malignancy that cause a mass of deaths world widely. We chose gene expression datasets of GSE27635 and GSE28248 from GEO database to find out key genes and their interaction network during the progression and metastasis of HCC. GEO2R online tool was used to screen differentially expressed genes (DEGs) between tumor and peri-tumor tissues based on these two datasets. The identified differentially expressed genes were prepared for further analysis such as GO function, KEGG pathway, PPI network analysis using Database for Annotation, Visualization and Integrated Discovery (DAVID) and Retrieval of Interacting Genes (STRING). Two modules were constructed by MOCDE plugin in Cytoscape and 21 genes were selected as hub genes during this analysis. The expression heatmap and GO function of hub genes were performed using R pheatmap package and BiNGO plugin in Cytoscape respectively. Six hub genes including CDC25 A, CDK1, HMMR, MYBL2, TOP2A were recollected for survival analysis and their expression was validated using Kaplan Meier-plotter and GEPIA website. We also investigated the DEGs between metastasis and non-metastasis tissues and two genes (NQO1 and PTHLH) are highly associated with the metastasis in HCC. Further verification using woundhealing and transwell assay confirmed their ability to mediate cell migration and invasion. In summary, our results obtained by bioinformatic analysis and experimental validation revealed the dominant genes and their interaction networks that are associated with the progression and metastasis of HCC and might serve as potential targets for HCC therapy and diagnosis.     

功能性鼻内镜手术治疗霉菌性鼻窦炎33例疗效分析

目的：探讨功能性鼻内镜技术在霉菌性鼻窦炎治疗中的作用及疗效。方法对功能性鼻内镜手术治疗的33例临床资料及随访结果进行回顾性分析。结果全组33例均一次性治愈，随访1~3年无复发。结论功能性鼻内镜手术治疗霉菌性鼻窦炎疗效满意，值得推广。     

肝源性糖尿病38例临床分析

目的:探讨肝源性糖尿病的发病机制、临床特点及治疗原则。方法:对38例肝源性糖尿病的临床资料进行回顾性分析。结果:38例患者在诊断糖尿病之前均有慢性肝病史,全部病例在护肝治疗基础上分别予以降糖治疗,肝功能逐渐好转。结论:肝源性糖尿病的临床表现以肝病为主,糖尿病的症状多不典型。积极治疗原发病,辅以降糖治疗,以胰岛素为主,糖尿病可随肝病好转而恢复。     

腹腔镜下袖状胃切除术治疗高体重指数2型糖尿病的效果分析

目的探讨腹腔镜袖状胃切除术(LSG)治疗高体重指数2型糖尿病(T2DM)患者的可行性及疗效的稳定性。 方法回顾性分析2010年至2016年间两个医院24例实施LSG的T2DM患者的临床资料,所有数据建立完整的excel表格,采用SPSS 17.0分析。术前术后体重和糖化血红蛋白(HBALc)水平以( ±s)表示,采用t检验;分类数据以频率和百分比表示,采用卡方检验。P<0.05认为差异有统计学意义。 结果24例患者平均基线体重为(117.2±30.2) kg,在术后3个月、6个月、12个月和24个月时显著下降至(100.3±22.1) kg、(91.6±22.4) kg、(81.3±16.5) kg和(80.3±14.3) kg, P<0.001。术前平均基线HbA1c为(8.5±2.2)%,分别在3个月、6个月、12个月和24个月显著降低至(6.4±1.6)%,(5.8±1.1)%, (5.7±0.7)%和(5.3±0.6)% P<0.001。19例接受袖状胃切除术的T2DM患者停止使用胰岛素和口服降糖药,根据血糖控制标准,24例患者24个月缓解率达79.2%。除1例继续使用2种降压药外,其余高血压患者(17例)停止降压药治疗。 结论LSG治疗高体重T2DM患者,方法简单,手术安全,治疗效果确切,值得在临床中普遍推广应用。     

Hypoxia-induced MTA1 promotes MC3T3 osteoblast growth but suppresses MC3T3 osteoblast differentiation

Background

Bone fracture is one of the most common physical injuries in which gene expression and the microenvironment are reprogramed to facilitate the recovery process.

Methods

By specific siRNA transfection and MTT assay, we evaluated the effects of metastasis-associated gene 1 (MTA1) in osteoblast growth. To show the role of MTA1 in osteoblast under hypoxia conditions, by overexpressing and silencing MTA1 expression, we performed mineral deposition and alkaline phosphatase activity assay to observe the differentiation status of osteoblast cells. Real-time PCR and Western blot assays were adopted to detect the expression of certain target genes.

Results

Here, we reported that hypoxia-induced MTA1 expression through hypoxia-induced factor 1 alpha (HIF-1α) and stimulated the growth of osteoblast MC3T3 cells. Silencing of MTA1 through specific siRNA suppressed MC3T3 cell growth and elicited cell differentiation and induced alkaline phosphatase activation and the upregulation of bone morphogenetic protein-2 and osteocalcin.

Conclusions

We found that MTA1 was regulated by HIF-1α in hypoxia circumstance to suppress osteoblast differentiation. These findings provide new insights for bone fracture healing and new strategies to develop potential targets to promote fracture healing.

Electronic supplementary material

The online version of this article (doi:10.1186/s40001-015-0084-x) contains supplementary material, which is available to authorized users.     

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??OBJECTIVE To prepare luteolin-loaded Solutol hs15/Poloxamer188 mixed micelles, evaluate their pharmaceutical property and study their anti-tumor activity. METHODS The luteolin-loaded mixed micelles were prepared by thin-film hydration method. The particle size was measured by laser granulometry, and the morphology was observed under transmission electron microscope (TEM). The in vitro release behavior was determined by the dialysis method. Cell toxicity and uptake assays were employed to evaluate the anti-tumor activity. The targeting effect was studied by fluorescence labeling. RESULTS The prepared luteolin-loaded mixed micelles showed spherical and regular shape with an average particle size of 66.43 nm and its entrapment efficiency was more than 80%. The 12 h-accumulated release ratio in vitro was up to 97.42%. The cytotoxicity of luteolin-loaded mixed micelles on human lung adenocarcinoma cell line A549 was significantly stronger than that of free luteolin and the uptake rate was improved. After injecting DiR-loaded mixed micelles into nude mouse, the drug begun to accumulate in tumors after 2 h and reached the highest concentration at 6 h which was significantly higher than that in other tissues. There remained strong drug distribution in the tumors after 12 h. CONCLUSION The Solutol hs15/ Poloxamer188 mixed micelles have small particle size and high entrapment efficiency and can enhance cell toxicity and uptake with certain passive targeting effect. It is a promising delivery system for anti-tumor drug.     

思维导图在降低住院患者静脉血标本不合格率中的应用

目的探讨思维导图在降低住院患者静脉血标本不合格率中的应用效果。 方法通过对宿迁市第一人民医院2016年7月至2017年6月静脉血标本不合格信息进行收集统计,讨论分析降低静脉血标本不合格率关键节点,制定静脉血标本采集思维导图,比较采用思维导图改进前后6个月血标本采集执行流程的不合格问题与改进前后静脉血标本合格率情况。 结果应用思维导图后血标本采集执行流程中关键环节的不合格问题低于改进前(P<005),静脉血标本不合格率也低于改进前,差异均有统计学意义(P<005)。 结论应用思维导图能够规范静脉血标本采集流程与送检流程,降低静脉血标本不合格率。     

支气管类癌的外科诊治（附11例报告）

目的总结分析支气管类癌的临床特点、诊断、治疗方式方法及其疗效。方法回顾性分析697例肺部肿瘤中11例类癌患者的临床资料,并复习文献进行讨论。结果本组11例患者均行手术治疗,其中9例行肺叶切除术,2例行袖状切除术;11例患者术后病理检查确诊为支气管类癌;全组无围手术期死亡。结论支气管类癌的临床症状缺乏特征性改变,最终确诊须靠病理检查,手术治疗预后较好。