Chlorella sp.-ameliorated undesirable microenvironment promotes diabetic wound healing

Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments, such as high-glucose, excessive reactive oxygen species (ROS), hypoxia and malnutrition. Unfortunately, few strategies have been developed to ameliorate the multiple microenvironments simultaneously. In this study, Chlorella sp. (Chlorella) hydrogels were prepared against diabetic wounds. In vitro experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis, actively consume glucose and deplete ROS with the inherent antioxidants, during the daytime. At night, Chlorella was inactivated in situ by chlorine dioxide with human-body harmless concentration to utilize its abundant contents. It was verified in vitro that the inactivated-Chlorella could supply nutrition, relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration. The advantages of living Chlorella and its contents were integrated ingeniously. The abovementioned functions were proven to accelerate cell proliferation, migration and angiogenesis in vitro. Then, streptozotocin-induced diabetic mice were employed for further validation. The in vivo outcomes confirmed that Chlorella could ameliorate the undesirable microenvironments, including hypoxia, high-glucose, excessive-ROS and chronic inflammation, thereby synergistically promoting tissue regeneration. Given the results above, Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.     

超高效液相色谱串联三重四极杆质谱法测定三种葡萄糖制剂中 5-羟甲基糠醛的含量

目的建立葡萄糖注射液、葡萄糖酸钙口服溶液与盐酸氨基葡萄糖胶囊中 5-羟甲基糠醛的超高效液相色谱串联三重四极杆质谱测定方法。方法 2021年 1—9月，采用 ACQUITY UPLC HSS T3(2.1 mm×100 mm，1.8 μm)色谱柱，流动相为 0.05%甲酸溶液 -甲醇( 85∶15)，流速为 0.3 mL/min，柱温 30 ℃，进样盘温度 5℃，进样体积 2 μL。质谱条件: ESI源，正离子扫描，脱溶剂温度: 400 ℃；干燥气流速: 15 L/min；毛细管电压: 3.0 kV。结果 5-羟甲基糠在 2.07～155.25 μg/L浓度范围内，线性关系良好( r=0.999 7)定量限为 0.36 μg/L，平均回收率为 99.54%，RSD为 1.63%(n=9)。结论该方法专属性较强，灵敏度高，稳定性     

血清Fetuin-A、NGAL对糖尿病肾病的早期诊断价值

目的 探讨糖尿病肾病（DN）患者血清胎球蛋白A(Fetuin-A）、中性粒细胞明胶酶相关脂质运载蛋白（NGAL）变化及早期诊断DN的价值。方法 选取2018年9月—2021年12月徐州医科大学附属宿迁医院收治的73例DN患者为研究组，另选取同期该院收治的单纯糖尿病患者61例为对照组，研究组按病情严重程度又分为正常白蛋白尿DN组、微量白蛋白尿DN组及大量白蛋白尿DN组。收集比较研究组与对照组患者的基本资料。采用酶联免疫吸附试验检测血清Fetuin-A、NGAL水平。采用多因素逐步Logistic回归模型分析影响糖尿病患者发生DN的因素。绘制受试者工作特征（ROC）曲线分析血清Fetuin-A、NGAL诊断DN的价值。结果 研究组UAER、血肌酐、CysC、Fetuin-A、NGAL高于对照组（P<0.05）。大量白蛋白尿DN组的Fetuin-A、NGAL水平高于正常白蛋白尿DN组和微量白蛋白尿DN组（P<0.05），微量白蛋白尿DN组的Fetuin-A、NGAL水平高于正常白蛋白尿DN组（P<0.05）。多因素逐步Logistic回归分析显示，尿白蛋白排泄率■是影响糖尿...     

内镜下使用改良自制尿道延长器行尿道肉阜电切术的初步体会

目的 探讨使用改良自制尿道延长器，对老年女性尿道肉阜患者行内镜下等离子电切术的临床效果。方法 2019年1月－2022年12月该院共收治尿道肉阜患者28例，均于改良自制尿道延长器辅助下行等离子电切术，分析患者手术时间、住院时间、导尿管留置时间和术后并发症发生等情况。结果 该组28例患者，均在自制尿道延长器辅助下顺利完成手术，无中转开放手术，手术时间15～40 min，平均（24.4±6.4）min；导尿管留置时间3～7 d，平均（5.1±1.1）d；住院时间4～8 d，平均（6.5±1.0）d；拔除导尿管后，均排尿通畅，无尿失禁和排尿困难等近期并发症发生，病理均提示尿道肉阜；随访时间2～22个月，平均（11.8±6.5）个月，无尿道狭窄和尿道肉阜复发等远期并发症发生。结论 内镜下使用改良自制尿道延长器治疗尿道肉阜，安全、有效。值得临床推广应用。