寻常性银屑病患者表皮中DNA甲基化转移酶2和3a mRNA的表达

目的 检测寻常性银屑病患者表皮中DNA甲基化转移酶（DNMT2和DNMT3a）mRNA表达。方法 利用实时定量PCR技术检测2009年3月至2010年12月来自中国医学科学院皮肤病医院皮肤科及宜兴市人民医院皮肤科门诊的46例寻常性银屑病患者皮损和非皮损表皮以及来自中国医学科学院皮肤病医院皮肤外科的28例健康对照表皮中DNMT2和DNMT3a mRNA的表达。结果 在银屑病患者皮损、非皮损和对照组表皮DNMT2 mRNA表达水平（2-ΔΔCt值）分别是0.62 ± 0.02、0.36 ± 0.05和0.15 ± 0.11,皮损组明显高于非皮损组（t = 6.23,P < 0.01）,非皮损组明显高于对照组（t = 7.33,P < 0.01）;DNMT3a mRNA表达水平（2-ΔΔCt值）分别是0.85 ± 0.03、0.43 ± 0.04和0.18 ± 0.09,皮损组明显高于非皮损组（t = 5.66,P < 0.01）,非皮损组明显高于对照组（t = 8.62,P < 0.01）。结论 寻常性银屑病患者表皮DNMT2和DNMT3a mRNA均异常高表达。     

Single-dose and multiple-dose pharmacokinetics of zaltoprofen after oral administration in healthy Chinese volunteers

Zaltoprofen, a propionic acid derivative of non-steroidal anti-inflammatory drugs, has strong inhibitory effects on actue and chronic inflammation. A randomized, dose-escalating study was conducted to evaluate the pharma-cokinetics of single and multiple oral doses of zaltoprofen in 12 healthy Chinese volunteers. Pharmacokinetics was determined from serial blood samples obtained up to 24 h after administration of a single dose of zaltoprofen at 80, 160 or 240 mg and after multiple doses of zaltqorofen at 80 mg 3 times daily. The C max and AUC 0-24 of zaltoprofen were found to be proportional to drug dose. Zaltoprofen was rapidly absorbed (t max =1.46±0.83 h) and cleared (t 1/2 =4.96±2.97 h). Pharmacokinetic parameters after multiple doses were similar to those after single doses. Zaltoprofen was well tolerated. These results support a tid regimen of zaltoprofen for the management of acute and chronic inflammation.     

不同pH值对几种常见暗色致病真菌生长的影响

目的观察不同pH值对几种暗色致病真菌生长的影响。方法将裴氏着色真菌、紧密着色真菌、疣状瓶霉、卡氏支孢霉、皮炎外瓶霉、甄氏外瓶霉、假阿利什菌、链格孢和申克氏孢子丝菌接种在pH值为1～14的沙氏培养基上,观察其生长情况。结果几种着色真菌在pH值为5、6、7的沙氏培养基上均生长良好;疣状瓶霉、皮炎外瓶霉适宜生长的pH值范围较广,在pH值为4～12的培养基上均生长良好;在pH值为1、2、14的培养基上以上6种真菌均不生长。假阿利什菌在pH4～14的培养基上可生长,链格孢、申克氏孢子丝菌在pH3～14的培养基上可生长。结论 pH值对几种着色真菌、外瓶霉、假阿利什菌、链格孢和申克氏孢子丝菌的生长均有不同程度的影响。皮炎外瓶霉、疣状瓶霉较裴氏着色真菌、卡氏支孢霉适宜生长的pH值范围广。     

Preclinical anti-angiogenesis and anti-tumor activity of SIM010603, an oral,multi-targets receptor tyrosine kinases inhibitor

Objective  

SIM010603 is a structurally novel, oral, multi-targeted receptor tyrosine kinase inhibitor. This study investigated the anti-angiogenic and anti-tumor effects of SIM010603.     

江苏省流动妇女心理健康状况及影响因素分析

目的:了解居住地在江苏的流动妇女心理健康状况和影响心理健康的因素。方法:采用症状自评量表(SCL-90)和社会支持评定量表对1 178例流动人口进行调查,其中流动妇女729例。结果:①流动妇女总分、躯体化、焦虑、敌对、恐怖、精神病性因子的均分高于中国普通人群常模,在躯体化、恐怖、精神病性因子与中国常模差异有统计学意义(P0.001)。②流动妇女总分和各因子均分除偏执因子外均高于男性流动人口,躯体化、人际关系敏感、焦虑、恐怖和总分与男性流动人口差异有统计学意义(P0.05)。③按标准化偏回归系数显示影响流动妇女心理健康的因素为每天工作时间、是否签订劳动合同、客观支持、尚未找到工作、对生活状况满意程度、打零工和暂时不打算工作。结论:流动妇女存在一定程度的心理健康问题。     

Anti-Helicobacter pylori compounds from the ethanol extracts of Geranium wilfordii

Ethnopharmocological relevance

Geranium wilfordii Maxim has been extensively used in Chinese Herbal Medicine for treating gastrointestinal disorders, diarrhea and dysentery. In the current study we aimed to investigate the anti-Helicobacter pylori activity of ethanol extracts of Geranium wilfordii Maxim and its main active compounds, corilagin and 1,2,3,6-tetra-O-galloyl-β-d-glucose.

Materials and methods

The plant materials were extracted three times with ethanol and the concentrated filtrate was successively fractioned into chloroform, ethyl acetate, and n-BuOH-soluble portions which were examined in vitro for the anti-Helicobacter. pylori activity. Employing a standard strain and five clinical isolates of Helicobacter pylori, the extract, fractions and compounds of Geranium wilfordii Maxim were assessed in vitro.

Results

The ethanol fraction, ethyl acetate fraction, corilagin, and 1,2,3,6-tetra-O-galloyl-β-d-glucose were found to be strongly inhibitory to Helicobacter. pylori (MICs: 40, 30, 4, and 8 μg/ml respectively).

Conclusions

The results of the present study showed that the ethanol and the ethyl acetate extracts from Geranium wilfordii Maxim displayed as well the most significant inhibition to the growth of Helicobacter. pylori, of which corilagin and 1,2,3,6-tetra-O-galloyl-β-d-glucose have been identified main anti-Helicobacter pylori active constituents.     

银杏叶类脂成分与聚戊烯醇的协同抑菌作用

目的:考察银杏叶聚戊烯醇同系物以及其他15种类脂成分单体化合物对5种菌种(沙门菌、金黄色葡萄球菌、黑曲霉菌、大肠杆菌和枯草芽孢杆菌)的抑菌及协同作用.方法:正己烷作溶剂和空白对照,在样品质量浓度500 mg· L-1下,利用滤纸片法测定样品抑菌圈值;样品用正己烷配制成质量浓度分别为250,125,62.5,31.3,15.6,7.8,3.9 mg·L-1溶液并分散在培养基中,记录无菌生长样品浓度,以确定最小抑菌浓度(MIC)、最小杀细菌浓度(MBC)和最小杀真菌浓度(MFC)和计算分级抑菌浓度指数(FIC)方法来比较各样品对菌种的抑制作用.结果:橙花叔醇对4种细菌(沙门菌、金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌)的抑菌活性均为最强,抑菌圈范围为17.4～20.9 mm,MIC和MBC范围依次为3.9 ～15.6,31.3～62.5 mg·L-1;芳樟醇对黑曲霉菌的抑菌活性最强,抑菌圈为17.9 mm,MIC和MFC依次为7.8,31.3 mg·L-1;β-石竹烯与银杏叶聚戊烯醇混合样品组对枯草芽孢杆菌的协同抑制作用最强;当β-石竹烯与银杏叶聚戊烯醇质量比为43.42％∶ 56.58％时,其抗枯草芽孢杆菌协同作用最佳,此时测得实际值FIC指数为0.16,抑菌圈值为19.8 mm.结论:银杏叶类脂成分具有一定抑菌作用,部分类脂成分与聚戊烯醇混合具有协同抑菌效应.     

某家具厂喷漆作业场所有害气体通风防护措施分析与建议

[目的]掌握木制品企业喷漆场所职业病危害现状,了解防护设施有效性,改善工作场所劳动条件,提高防护水平。[方法]通过现场调查,定量和定性分析有害气体通风防护措施状况。[结果]喷漆工作场所空气中二甲苯浓度普遍超标,超标率为83％。有8名喷漆人员接触苯系物的相关体检项目指标异常,占接触人数的10％。喷漆台排风罩和喷漆房侧吸罩前1m处的风速分别为0．2～0．3、0．3～0．4m／s,通风防护效果差。[结论]原喷漆作业通风防护装置存在结构性缺陷。经采取改良措施,设计整体密闭层流通风罩,并在其与外界相贯通的出入口设置负压气锁,既可提高对罩内操作人员的防护效果,又可控制对罩外的污染。     

头颈部皮肤鳞状细胞癌的外科治疗进展

皮肤鳞状细胞癌多发生于头颈部,由于头颈部的淋巴管引流通路丰富且变化较多,易发生颈淋巴结和腮腺的转移,因此,主要的治疗方法为手术切除和（或）加术后放疗.肿瘤巨大向深部组织侵袭易使周围神经受累.腮腺和颈部淋巴结肿大和免疫抑制等提示预后不良.术前前哨淋巴结活检和选择性淋巴结清扫有助头颈部鳞状细胞癌分期、判断预后和后续治疗,提高患者生存率.     

动态浊度法定量检测防冻剂二甲基亚砜的细菌内毒素

目的探讨对二甲基亚砜（DMSO）进行细菌内毒素定量检测的方法。方法按照2005年版《中国药典》附录中的“细菌内毒素动态浊度法”,制定DMSO供试品内毒素的限值,通过干扰试验确定供试品的稀释浓度,并建立标准曲线对供试品的细菌内毒素进行定量检测。结果供试品稀释浓度0.1249EU/ml,对内毒素检查无干扰,同时内毒素回收率为50%～200%。结论防冻剂DMSO可采用动态浊度法进行细菌内毒素定量检测。