RP-HPLC法测定甲硫氨酸维B1注射液的含量

目的:用HPLC法同时测定复方甲硫氨酸维B1注射液中甲硫氨酸和维生素B1的含量,为复方甲硫氨酸维B1注射液的含量测定提供检测方法,制定质控标准.方法:用十八烷基键合硅胶为填充剂;0.005 mol·L-1庚烷磺酸钠溶液(pH=3.0)与乙腈为流动相,梯度洗脱;检测波长为210 nm;柱温30℃;流速约为1.0 mL·min-1.结果:甲硫氨酸与维生素B1分别能与相邻杂质峰较好分离,甲硫氨酸的浓度在0.10～2.10 mg·mL-1范围内线性相关性良好,维生素B1的浓度在0.011～0.21 mg·mL-1范围内线性相关性良好.结论:该法简便、准确、专属性好,可以用于甲硫氨酸维B1注射液的含量测定.     

局部外用酞丁安软膏后皮内浓度测定

目的测定试验动物皮肤给药后的皮内药物浓度,观察受试制剂的经皮渗透及进入皮肤的药物水平。方法试验乳猪局部外用酞丁胺软膏后,采用匀浆法结合高效液相色谱测定皮内药物浓度。结果酞丁安软膏外用于皮肤后,有一定量的经皮吸收和渗透,但是吸收量相对较低。结论本试验结果可为酞丁安人体皮肤药代动力学研究提供参考。     

麻风病受累者对社区护理需求的调查与分析

目的了解麻风病受累者对社区护理的需求。方法自行设计麻风病受累者对社区护理的需求调查表,对121例麻风病受累者进行面对面调查。结果 121例麻风病受累者中,93例有基础护理的需求,86例有健康教育的需求,62例有康复护理的需求。结论麻风病受累者对社区护理需求迫切,需大力开展麻风病受累者需求的社区护理。     

无偿献血者队伍建设与招募保留探讨

无偿献血者五支队伍建设与招募保留是无偿献血事业可持续发展的根本。结合工作实践,介绍了固定献血者队伍、应急献血者队伍、稀有血型献血者队伍、成分献血者队伍以及志愿者服务队伍的建立与招募保留的做法。     

1例大面积单侧型疣状痣的激光治疗及护理

疣状痣又称线状表皮痣、疣状线状痣等。因表皮细胞发育过度引起表皮局限性发育异常所致。通常在初生时或幼儿期发病，表现为淡黄色至棕黑色疣状损害，其大小、形态及分布各不相同，开始为小的角化丘疹，逐渐扩大，呈密集的角化过渡性丘疹，灰白色或黑色，触之粗糙坚硬，皱襞处损害常饵浸渍面较软。病变可位于身体任何部位，一般无自觉症状。小面积的皮损可用手术切除，较大面积可磨削去除皮损，但大面积的疣状痣在磨削及术后护理上仍有难度。     

Alu cell-free DNA concentration,Alu index,and LINE-1 hypomethylation as a cancer predictor

IntroductionThe liquid biopsy approach, a less-invasive diagnostic tool, enables the detection of disease-specific genetic and epigenetic aberrations. Approximately 66–69% of the human genome may be composed of transposable repetitive elements, including Alu and LINE-1. This study aimed to investigate whether Alu-derived cell-free DNA (cfDNA) concentrations, Alu index, and LINE-1 methylation could be used to distinguish patients with cancers from healthy individuals.MethodsTwo sets of primers, shorter and longer Alu fragments, were used to amplify Alu elements, followed by the quantitation of Alu DNA concentration and its integrity index. LINE-1 methylation status was then analyzed with quantitative PCR using methylation- and unmethylation-specific TaqMan probes.ResultsBoth Alu index and LINE-1 methylation level were significantly different in comparison between patients with lung or breast cancer and the healthy controls. The area under the ROC curve of the Alu index and LINE-1 hypomethylation was 0.742 and 0.848 for lung cancer, respectively, and 0.724 and 0.890 for breast cancer, respectively. However, Alu longer fragment DNA concentration was significantly correlated with Alu index in comparison to LINE-1 hypomethylation. Regression analysis suggested that the LINE-1 methylation level, rather than the Alu index, was a good discriminator for lung and breast cancers.ConclusionsThis study investigated the genome-wide Alu index and LINE-1 methylation status; their associations with cancers suggested that these combinatory panels could be implemented as a triage test to discriminate cancer patients from healthy individuals.     

中医药科研的现状分析及决策创新要略思 考简

从中医药科研的现状,对新中国成立后,特别是改革开放以来,我国中医药在科研战略和制度建设、人才培养、重大防病治病研究基中医药资源产业发展等现状进行分析.对其中存在的问题进行归类分析,并进行了决策创新要略思考.