6种支原体的检测在一般人群和非淋菌性尿道炎患者中的调查研究

目的为了探讨一般人群和非淋菌性尿道炎(NGU)患者在咽部和尿道(宫颈)的解脲脲原体(Uu)、人型支原体(Mh)、生殖支原体(Mg)、穿透支原体(Mpe)、发酵支原体(Mf)及梨支原体(Mpi)的携带情况,其致病范围、分布特点及临床意义。方法采集151名体检人员及65例NGU患者的咽部和尿道(宫颈)分泌物,应用套式PCR法进行检测,靶基因分别为6种支原体的16SrRNA基因的特异性片断。结果体检人员咽拭子标本6种支原体的总阳性率为9.9%,而NGU患者的则为47.7%,两类人员支原体阳性率有非常显著性差异(P<0.0001)。性别的支原体阳性率差异无显著性(P>0.05)。两类人群中有12例(5.6%)在咽部和尿道(宫颈)检出同种支原体,两类人群在两个部位检出同种支原体的阳性率也有显著性差异(P<0.01)。结论一般人群中咽部可能携带常见支原体,但携带率很低。Mg感染可能是NGU常见的致病因素。Mpe感染也值得引起重视。提示两部位检出同种支原体的现象可能是由于口交性行为引起,值得进一步研究。     

动员医疗机构参与性病艾滋病防治工作的效果评价

目的评估性病门诊医务人员参与性病艾滋病干预工作,针对不同高危人群提供性病、生殖健康服务的效果。方法选择卫九、艾滋病综合防治示范区和全球基金艾滋病防治项目地区有条件的医疗机构,根据性病门诊规范服务的要求,改善门诊服务,开展针对门诊就诊者的干预服务和现场高危人群的医疗服务。结果在三个项目中,医务人员规范化服务培训率、性病门诊干预服务提供率和外展服务性病门诊医务人员参与率,均有了不同程度的提高全球基金项目中,门诊主动提供艾滋病检测咨询(PITC)服务率从基线的10.4%,上升到项目后的96.4%。示范区和全球基金项目中,高危人群性病预防知识知晓率,分别由基线的31.02%、45.52%上升到72.40%和87.86%。结论从三个项目的实施情况来看,动员医疗机构主动参与到针对高危人群的性病、生殖健康干预工作,并提供转介服务是可行的,而且取得了较好的效果。     

Attenuation of Left Ventricular Adverse Remodeling With Epicardial Patching After Myocardial Infarction

BackgroundPrevious studies suggested that epicardial patch applied to the infarcted site after acute myocardial infarction (MI) can alleviate left ventricular (LV) remodeling and improve cardiac performance; however, the effects of regional epicardial patch on chronic phase of LV remodeling remain unclear.Methods and ResultsWe studied 20 pigs with MI induced by distal embolization and impaired LV ejection fraction (LVEF <45%) as detected by gadolinium-enhanced cardiac magnetic resonance imaging (MRI). Eight weeks post-MI, all animal underwent open chest procedure for sham surgery (control, n = 12) or patch implantation over the infarcted lateral LV wall (patch group, n = 12). In the patch group, +dP/dt increased and LV end-diastolic pressure decreased at 20 weeks compared with immediately post-MI and at 8 weeks (P < .05), but not in the control group (P > .05). As determined by cardiac MRI, LV end-diastolic and end-systolic volumes increased at 20 weeks compared with 8 weeks in both groups (P < .05). However, the increase in LV end-diastolic volume (+14.1 ± 1.8% vs. +6.6 ± 2.1%, P = .015) and LV end-systolic volume (+12.1 ± 2.4% vs. ?4.7 ± 3.7%, P = .0015) were significantly greater in the control group compared with the patch group. Furthermore, the percentage increase in LVEF (+17.3 ± 4.9% vs. +4.1 ± 3.9%, P = .048) from 8 to 20 weeks was significantly greater in the patch group compared with the control group. Histological examination showed that LV wall thickness at the infarct region and adjacent peri-infarct regions were significantly greater in the patch group compared with the control group (P < .05).ConclusionRegional application of a simple, passive synthetic epicardial patch increased LV wall thickness at the infarct region, attenuated LV dilation, and improved LVEF and +dP/dt in a large animal model of MI.     

妇产科住院患者红细胞输注的临床观察

目的观察妇产科住院患者进行红细胞输注的情况,探讨其临床应用价值。方法选取2011年1月～2012年12月我院妇产科符合红细胞输注指征的94例患者,根据疾病类型的不同分为4组:产科正常妊娠组(n=20)、产科病理妊娠组(n=32)、妇科肿瘤组(n=22)、妇科非肿瘤组(n=20)。4组患者基线资料无显著差异,具有可比性(P>0.05)。采用日本SYSMEX-XE2100全自动血细胞分析仪,比较4组患者红细胞输注前与输注后24 h的Hb含量。结果 4组患者红细胞输注后24 h的Hb含量较输注前均显著升高,差异均有统计学意义(P均<0.01)。其中产科正常妊娠组输注后24 h的Hb含量较产科病理妊娠组升高,差异有统计学意义(P<0.05);妇科肿瘤组输注后24 h内Hb含量高于妇科非肿瘤组,差异有统计学意义(P<0.05)。94例患者输注后临床均未见溶血性输血反应。结论合理及时地输注红细胞能迅速、有效地提高妇产科住院患者的Hb水平,对于妇产科治疗具有十分重要的临床应用价值。     

血站继续医学教育的探讨

规范血站的继续医学教育并加强管理是全面提高员工队伍整体素质的重要措施。文章通过倡导终生教育,实行单位、科室、个人三级管理,使培训工作覆盖率达到100％,确保员工得到了持续有效的教育和培训。     

低温减压沸腾提取橄榄苦苷的工艺研究

目的:对油橄榄叶进行低温减压沸腾提取橄榄苦苷以期获得提取的优化工艺。方法:根据单因素实验设计,考察了溶剂种类、温度、时间、料液比、乙醇浓度、真空度、提取次数7个因素对橄榄苦苷提取得率的影响;并在单因素实验基础上,选取了影响橄榄苦苷得率更重要的4个因素,分别为温度、时间、料液比及乙醇浓度,进行L9(34)正交试验,再与传统提取方法进行了对比。结果:通过直观分析及方差分析,4个因素都极显著,减压沸腾提取效果更佳。结论:橄榄苦苷低温减压沸腾提取优化条件为温度60℃,时间20 min,料液比1∶30,乙醇浓度85%,并在此优化条件下橄榄苦苷提取得率为5.90%。与传统提取方法及超声辅助提取相比,减压沸腾提取工艺具有时间短,效率高,并具有良好的应用前景。     

The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles

Objective

Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin (HSA) magnetic nanoparticles (Folate-CDDP/HSA MNP), we prepared drug-loaded Folate-CDDP/HSA MNPs and characterized their features.

Methods

First, folate was conjugated with HSA under the effect of a condensing agent, and the conjugating rate was evaluated by a colorimetric method using 2, 4, 6 - trinitrobenzene sulfonic acid. Second, under N₂ gas, Fe₃O₄ magnetic nanomaterials were prepared and characterized by using transmission electron microscopy (TEM), SEM-EDS and X-ray diffraction (XRD). Finally, Folate-CDDP/HSA MNP was prepared by using a solvent evaporation technique. TEM was used to observe particle morphology. The particle size and distribution of the prepared complexes were determined by a Laser particle size analyzer. Drug loading volume and drug release were investigated by a high performance liquid chromatography method (HPLC) in vitro.

Results

We successfully prepared folate-conjugated HSA and its conjugating rate was 27.26 µg/mg. Under TEM, Fe₃O₄ magnetic nanoparticles were highly electron density and had an even size distribution in the range of 10-20 nm. It was confirmed by SEM-EDS and XRD that Fe₃O₄ magnetic nanoparticles had been successfully prepared. Under TEM, drug-loaded magnetic nanoparticles were observed, which had a round shape, similar uniform size and smooth surface. Their average size was 79 nm which was determined by laser scattering, and they exhibited magnetic responsiveness. Encapsulation efficiency was 89.75% and effective drug loading was calculated to be 15.25%. The release results in vitro showed that the half release time (t½) of cisplatin in cisplatin Solution and Folate-CDDP/HSA MNP was 65 min and 24 h respectively, which indicated that microspheres had an obvious effect of sustained-release.

Conclusion

Folate-CDDP/HSA MNPs were prepared successfully. The preparation process and related characteristics data provided a foundation for further study, including the mechanism of the nanoparticles distribution in vivo and their intake by tumor cells.     

针灸治疗肥胖病并发高血压116例临床观察

目的:观察针灸治疗肥胖病并发高血压的疗效.方法:将116例肥胖病并发高血压患者依据辨证分型采用耳体针结合施治.观察针灸治疗前后患者的症状、体征、肥胖指标、血压的变化.结果:针灸1疗程(1月)患者总有效率为63.79%,2疗程总有效率为78.45%,3疗程总有效率为90.52%.结论:针灸对肥胖并发高血压同时有减肥和降压的双重作用.针灸对肥胖并发高血压具有良好的疗效,而痰湿壅盛、肝火亢盛和阴虚阳亢3型显著优于阴阳两虚型.     

Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta

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