Mechanism of Chaihu Shugan Powder (柴胡疏肝散) for Treating Depression Based on Network Pharmacology

Objective: To analyze the effective components of Chinese medicine (CM) contained in Chaihu Shugan Powder (柴胡疏肝散, CSP) in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology. Methods: Absorption, distribution, metabolism, excretion, and toxicity calculation method was used to screen the active components of CSP. Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool (GoPuMed database) were used to predict and screen the active ingredients of CSP and anti-depressive targets. Through Genetic Association Database, Therapeutic Target Database, and PharmGkb database targets for depression were obtained. Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP, and to analyze gene function and metabolic pathways through Database for Annotation, Visualization and Integrated Discovery and the Omicshare database. Results: The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity, growth, transfer condition and gene expression of neuronal cell, and the raise of the expression of gap junction protein. The 15 targets passed 14 metabolic pathways, mainly involved in the regulation of neurotransmitters (5-hydroxytryptamine, dopamine and epinephrine), inflammatory mediator regulation of TRP channels, calcium signaling pathway, cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects. Conclusion: This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research, and lays a foundation for further target studies.     

从《温疫论》看新型冠状病毒疫情的中医学术内涵

从吴又可《温疫论》学术思想的角度,探讨了新型冠状病毒感染性疾病（COVID-19）的中医病名命名、病邪性质、病变部位以及疾病的传变和诊治等内容,提出可以汇集本次疫情中的大数据,以探讨病邪为轴线的瘟疫传变过程;进一步探索基于病邪-正气交争关系的诊治模式;基于现代医学实践的成果,结合伏邪匿于膜原的病位理论,给予伏邪、膜原以现代诠释。借鉴《温疫论》的学术观点,结合本次疫情的流行病学研究、临床病症的特征、相关实验室检查以及临床中医药的诊治经验,可以进一步发展和完善中医温病学的学术内涵。      

Time-Dependent Oxidative Stress Responses of Submerged Macrophyte Vallisneria natans Seedlings Exposed to Ammonia in Combination with Microcystin Under Laboratory Conditions

We studied the antioxidant responses of macrophyte Vallisnerria natans seedlings to combined ammonia (0, 0.21, and 0.85 mg L⁻¹) and microcystin-LR (MC-LR) (0, 10, and 50 μg L⁻¹) for 7 days. Results showed that superoxide dismutase, catalase (CAT), peroxidase, and glutathione were significantly induced by the mixtures of ammonia and MC-LR, and there were significant interactions between ammonia and MC-LR. Specially, CAT increased about fivefold at ammonia 0.85 mg L⁻¹ and MC-LR 50 μg L⁻¹ on day 3. Malondialdehyde fluctuated with both ammonia and MC-LR, and significant interactions were detected between the two stressors. Changes in all the measured variables were time-dependent.     

针药结合治疗脑梗死的规律性探讨

脑梗死是严重危害人类健康的主要疾病。但遗憾的是到目前为止,对该疾病的治疗还无明显突破,相对而言,针药结合在治疗中显示了较好的疗效。文献显示:针药结合治疗脑梗死的疗效优于单纯针刺或单纯药物组,但该结合目前还处于初级阶段,对其治疗的规律性尚不十分清楚。为此,探索性地提出了针药结合治疗脑梗死的思路:①超早期(6h内)应以溶栓药物为主,神经保护剂与针灸为辅;②急性期(48h内)治疗应神经保护剂与针灸并重;③恢复及后遗症期(48h后)治疗应以针灸为主,改善大脑代谢药物及中药为辅。     

盆底功能障碍性疾病多学科整合的必要性及其存在的问题

本文结合盆底疾病发展历程及本中心的临床实践体会，探讨盆底功能障碍性疾病多学科整合的价值及其存在的问题，以期提高对盆底功能多学科整合的认识并对其存在问题进行初步探讨。     

内生湿热证人群的肠道菌群特点研究

目的探讨内生湿热证人群与健康人群的肠道菌群差异性。方法选取2019年4—9月于江苏省第二中医院门诊就诊及住院的24例内生湿热证患者作为湿热组,选取同期8~14岁健康男童20例作为对照组,取2组粪便样本,通过Illumina MiSeq平台对肠道菌群进行16S rDNA基因V3~V4可变区检测,运用QIIME、STAMP及LEfSe进行Alpha、Beta多样性分析,鉴定差异性菌属。结果湿热组的Shannon指数、Evenness指数均明显低于对照组(P均<0.05)。主坐标分析显示2组样本菌群结构分布差异有统计学意义(P<0.05)。门分类水平上,湿热组拟杆菌门与厚壁菌门的相对丰度比值呈下降趋势,疣微菌门的相对丰度比值明显低于对照组(P均<0.05);属分类水平上,湿热组布劳特氏菌属、迟缓埃格特菌的相对丰度均明显高于对照组(P均<0.05);瘤胃球菌属、双歧杆菌属、Alistipes、颤螺菌属、Odoribacter、Anaerostipes、无色杆菌属的相对丰度均明显低于对照组(P均<0.05)。结论内生湿热证患者存在肠道菌群紊乱,其发病主要与拟杆菌门与厚壁菌门的相对丰度比值下调,迟缓埃格特菌属丰度上升,瘤胃球菌属、双歧杆菌属、颤螺菌属丰度下降相关。     

Both Notch1 and its ligands in B cells promote antibody production

Notch1 signaling regulates B and T lymphocyte development and also in vitro promotes antibody secretion upon B cell activation. However, it is still unclear about the role of Notch1 in antibody production upon in vitro and in vivo mixture lymphocytes activation. We first showed that Notch1 expressed in LPS-activated CD19^hi B cells and CD19^cre mediated Notch1 knock-down in LPS-activated B cells. Furthermore, we demonstrated that Notch1 knock-down in B cells reduced antibody production in LPS-stimulated B cells but did not affect antibody production in LPS-stimulated splenocytes and in experimental allergic encephalomyelitis (EAE) mice. Importantly, Notch1 ligands Dll1 and Jag1 expressed in B cells and pre-coated Notch1 protein promotes Notch1-knocked down B cells to produce antibody in LPS-stimulated B cells suggesting that Notch1 in other cells may promote antibody production by binding its ligands Dll1 and Jag1 in B cells. Together, our results suggest that both Notch1 and its ligands in B cells play an important role in antibody production.     

自体脂肪颗粒移植联合小针刀治疗面部老化的疗效分析

【摘要】 目的 探讨自体脂肪颗粒移植联合小针刀治疗面部老化的临床效果。方法 选取 2018 年 6 月至2020 年 6 月上海木心医疗美容门诊部收治的86例拟行面部年轻化治疗者作为研究对象,并按照随机数表法将其分为观察组(43例)与对照组(43例)。观察组研究对象采用自体脂肪颗粒移植联合小针刀治疗,对照组研究对象单纯采用自体脂肪颗粒移植治疗,对比两组研究对象移植脂肪成活率、面部皮肤生理指标、临床疗效以及不良反应发生情况。结果 术后3个月,观察组研究对象脂肪成活率明显高于对照组 ( t = 9.471,P < 0.001),面部皮肤黑色素值、红色素值均明显低于对照组 (t = 11.320、7.872,P 均 < 0.001),皮肤水合度明显高于对照组(t = 23.460,P < 0.001);观察组研究对象中显效28例、有效13例、无效2例,明显优于对照组研究对象的显效16例、有效15例、无效12例 (Z = - 3.048,P = 0.002)。两组研究对象术后均无明显不良反应。 结论 自体脂肪颗粒移植联合小针刀治疗面部老化,可明显提升脂肪成活率,改善面部皮肤生理指标,提高治疗效果,且安全性较高。     

中国和加拿大两国执业药师管理制度之比较

从中国和加拿大两国执业药师管理制度的异同,对中加两国执业药师的作用和社会地位进行了比较,并从个人理解的角度,指出了中国执业药师与加拿大执业药师之间的差距,提出了中国执业药师的努力方向。     

儿童咳嗽变异性哮喘中医病机病名之探讨

从病因、病机、病名等方面对儿童咳嗽变异性哮喘进行了探讨,认为主要病机是风动痰阻,肺失宣降,肺络气逆,气逆是其关键。指出应将咳嗽变异性哮喘归入中医"逆咳"为妥。