Differential expression of peroxiredoxin 6, annexin A5 and ubiquitin carboxyl-terminal hydrolase isozyme L1 in testis of rat fetuses after maternal exposure to di-n-butyl phthalate

ObjectivesTo isolate and identify differentially expressed proteins in testis of rat fetuses after maternal exposure to di-n-butyl phthalate (DBP).MethodsPregnant rats were daily treated by gavage with 1 ml/kg corn oil or 750 mg/kg DBP from GD14 to GD18. We used the technique of proteomic analysis to compare the testis protein patterns obtained by two-dimensional gel electrophoresis from fetal rats of gestation day 19.ResultsWe found significant differences in protein spot intensities compared to control. Subsequently several of these variant protein spots were identified by mass spectrometry. Peroxiredoxin 6 (Prdx6), annexin A5 (AnxA5) and ubiquitin carboxyl-terminal hydrolase isozyme L1 (UchL1) were three of them, the differential expression of which were confirmed by western blotting. Further, immunohistochemical analyses of fetal rat testes sections were made to determine the cellular distribution of these proteins, consequently strong Prdx6 and AnxA5 stainings were found primarily in Leydig cells, while a weak UchL1 staining was found primarily in spermatogonium.ConclusionsThe present study had found several differentially regulated proteins and demonstrated the differential expression of Prdx6, AnxA5 and UchL1 in fetal rat testis after maternal exposure to DBP, when compared with controls. Combining the cellular location of these proteins and their function in other tissues, the results of this study indicated that oxidative injury and abnormal apoptotic regulation might participate the formation of testicular dysgenesis in fetuses of dams exposed to DBP.     

Mallet骨折内固定方法的改进

目的：探讨Mallet骨折的治疗方法和临床疗效。方法2012年3月—12月，我们采用切开复位应用3枚克氏针内固定治疗Mallet骨折17例，采用Doyle分型，其中ⅣB型12例，ⅣC型5例。结果术后随访6～18个月，切口均一期愈合，17例骨折均愈合，愈合率100%，骨折愈合时间6～10周，平均9周，无并发症发生。采用 Dargan 法评估疗效，优14例，良2例，可1例，优良率94.12%。结论增加一枚加压克氏针内固定技术治疗Mallet 骨折，能稳定有效固定较小的骨折块，改善关节活动范围及降低术后关节疼痛，是治疗Mallet骨折的有效方法之一。     

巨细胞病毒肝炎婴儿外周血生长抑素、肠血管活性多肽与γ谷氨酰转肽酶水平的相关性

目的 探讨巨细胞病毒(CMV)肝炎婴儿血浆生长抑素(SST)、肠血管活性多肽(VIP)水平与血清γ谷胺酰转肽酶(γ-cT)的相关性,了解其在CMV肝炎婴儿持续性胆汁瘀积的诊断和鉴别诊断中的意义.方法 收集本院CMV肝炎婴儿60例.临床确诊依据CMV感染诊疗方案,即具备以下条件:(1)外周血或尿CMV-DNA和CMV-IgM呈阳性,甲型肝炎、乙型肝炎、丙型肝炎、丁型肝炎和戊型肝炎抗体检测均为阴性；(2)外周血ALT和结合胆红素(DB)高于正常值上限.根据最初的DB高低分组,≤136.8μ mol·L-1(瘀胆型肝炎I组)22例；＞ 136.8 μmol·L-1(瘀胆型肝炎Ⅱ组)20例,全部具备条件(1)和(2),影像学检查排除胆道闭锁(BA).BA组18例全部具备条件(1)和(2),并有B超检测胆道不显影及锝99放射扫描显像法肠道不显影.健康对照组14例.血浆SST或VIP水平测定采用放射免疫法,而血清γ-GT水平测定采用常规生物化学法.结果 健康对照组、瘀胆型肝炎Ⅰ组和瘀胆型肝炎Ⅱ组SST水平与γ-GT水平无相关性(r＝0.180,P＞0.05)；3组VIP水平与y-GT水平呈显著正相关性(r＝0.383,P＜0.05).健康对照组和BA组SST、VIP水平分别与γ-GT水平均呈显著负相关(r＝-0.743,P＜0.01;r＝-0.918,P＜0.01).结论 CMV肝炎患儿外周血VIP水平可作为反映肝内胆汁瘀积的良好指标.此外,外周血SST或VIP水平可作为瘀胆型肝炎和BA鉴别诊断的敏感指标之一.     

缺血性心力衰竭损害蛋白酶体功能

背景泛素蛋白酶体系统(UPS)是ATP依赖性的、非溶酶体蛋白质的降解通路,在扩张型心肌病、压力负荷引起的心力衰竭中泛素化蛋白积聚,存在着蛋白酶体功能损害,而在缺血性慢性心力衰竭中UPS的变化尚未见报道。目的研究UPS在缺血性慢性心力衰竭中的变化。方法雄性GFPdgn转基因小鼠,通过缝扎冠状动脉左前降支制作慢性心力衰竭模型,随机分为冠状动脉结扎术后1周组(MI-1)和3周组(MI-3),每组取16只制模成功小鼠进行实验。16只转基因小鼠开胸,撕开心包,不结扎冠状动脉,作为对照组。超声心动图测定心脏参数,荧光底物测定糜蛋白酶样(Ch-TL)活性,应用免疫荧光标记共聚焦显微镜法检测心肌组织泛素化蛋白,Westernblot法检测心肌组织泛素化蛋白和26S蛋白酶体亚单位蛋白表达水平。结果冠状动脉结扎后,左室舒张末内径(LVDd)、左室收缩末内径(LVDs)增加[LVDd:MI-1(0.37±0.05)、MI-3(0.40±0.07)比对照组(0.31±0.02)cm;LVDs:MI-1(0.28±0.03)、MI-3(0.31±0.05)比对照组(0.17±0.03)cm;P<0.05或0.01],短轴缩短率降低[M...     

Lack of evidence for association between DVWA gene polymorphisms and developmental dysplasia of the hip in Chinese Han population

Developmental dysplasia of the hip is the most frequent inborn deformity of the locomotor apparatus. Genetic factors play a considerable role in pathogenesis of Developmental dysplasia of the hip. Recently, several DVWA SNPs were found to be consistent and most significantly associated in Japanese and Han Chinese knee OA studies. Its functions may be associated with cartilage. It may be involved in etiology and pathogenesis of Developmental dysplasia of the hip. Our objective is to evaluate whether the DVWA SNPs (rs7639618, rs9864422 and rs11718863) are associated with Developmental dysplasia of the hip in Han Chinese. Three SNPs rs7639618, rs9864422 and rs11718863 (in DVWA) were genotyped using a Taqman 5′ allelic discrimination assay on an ABI 7500 real-time polymerase chain reaction (PCR) instrument in 368 children who suffered from Developmental dysplasia of the hip and 508 control subjects, and analyzed their associations. The genotype distribution and allele frequency were compared between Developmental dysplasia of the hip and healthy control. Neither genotype distributions nor allelic frequencies of the assayed single nucleotide polymorphisms were found to be significantly different between patients and controls. There was also no significant difference when the patients were stratified by sex or severity (all P > 0.05). Our results indicate that DVWA does not seem to be a risk factor for Developmental dysplasia of the hip etiology in Chinese Han population.     

江苏省人群代谢综合征的因子分析

目的代谢综合征（MS）是由于基因、激素和环境因素引起的疾病，它们间的关系可能在性别间存在差异。比较男女性MS中代谢疾病的差异。方法研究包括了2330名年龄在55．49±12．28岁，参加“江苏省多代谢异常和代谢综合征综合防治研究”前瞻性队列研究且进行了各种测量的人群，使用了因子分析中的主因子方法进行分析。结果研究发现了3个共性因子，处于核心的是肥胖因子，其次是血脂紊乱因子、高血压因子，而血压因子是独立的，而hs-CRP在男性中单独成为一独立因子，要比女性更明显。结论尽管MS的各组分是紧密相关的，发现了多个主因子提示了MS的发生可能有不止一个的病理机制。而且男性和女性中的致病机制可能也存在差异，建议可针对男、女性用不同的MS诊断标准。     

超声联合对比剂对内皮祖细胞一氧化氮生成的影响**◆

背景：已有动物实验证实超声联合对比剂能够显著增强骨髓干细胞心肌内归巢治疗心肌梗死，改善心功能。 目的：体外观察超声联合对比剂对内皮祖细胞一氧化氮生成及产生途径的影响。 方法：体外提取、分离和培养猪骨髓内皮祖细胞，随机分为对照组、超声组、超声对比剂组，干预后分别收集内皮祖细胞和上清液。采用硝酸还原法检测上清液一氧化氮水平，分光光度计检测内皮祖细胞一氧化氮合酶的相对活力。 结果与结论：超声对比剂组内皮祖细胞上清液一氧化氮表达、内皮祖细胞诱导型一氧化氮合酶和结构型一氧化氮合酶活力均较对照组显著增加(P < 0.05)；超声组一氧化氮水平无明显改变，各型一氧化氮合酶活力较对照组有下降趋势，但差异无显著性意义(P > 0.05)；超声联合对比剂作用下，L-精氨酸和双氧水可反应生成一氧化氮，较对照组明显增加，而单纯超声无此促进作用。提示超声联合对比剂作用显著增强内皮祖细胞各亚型一氧化氮合酶活性，并可能同时促进内皮祖细胞一氧化氮非酶合成途径，是其增效骨髓干细胞治疗心肌梗死的机制之一。     

化疗对骨肉瘤细胞Ki-67及p53表达的影响及其临床病理相关性

目的：探讨术前化疗对骨肉瘤肿瘤细胞增殖活性及p53表达的影响。方法：应用组织芯片技术及免疫组化法检测58例骨肉瘤患者（其中包括25例化疗后患者）标本中l（i-67及p53的表达情况。结果：Ki-67的表达在化疗后较化疗前明显下降（P〈0．05）；化疗后p53的表达略有下降，但无统计学意义（P〉0．05）；化疗后患者的硒-67及p53表达与患者的性别、发病部位、组织学类型、肿瘤大小、软组织侵袭及化疗反应均无明显相关性。结论：Ki-67可很好反映骨肉瘤细胞的增殖能力，术前化疗能有效抑制肿瘤细胞的增殖能力；术前化疗对p53的阳性表达率几乎无影响。     

睾丸胚胎性横纹肌肉瘤临床诊治(附3例报告)

目的:提高对睾丸胚胎性横纹肌肉瘤的认识、诊断与治疗水平。方法:回顾性分析3例睾丸胚胎性横纹肌肉瘤患者的临床病理资料,并复习相关文献。结果:3例患者均行根治性睾丸切除术,1例临床分期为Ⅱb期,该患者术后行放疗及化疗,术后9个月后出现全身转移而死亡;2例为Ia期,其术后均行化疗,分别随访32、3个月均未见复发和转移。结论:早期诊断,根治性手术并辅助化疗及放疗是提高患者生存率及延长生存时间的有效手段。     

石见穿有效部位对血管内皮细胞生物学行为的影响

目的:探讨石见穿氯仿及乙酸乙酯部位在体外对血管内皮细胞生物学行为的影响。方法:培养人脐静脉内皮细胞(HUVEC)至3-6代,采用MTT实验、Transwell小室迁移实验、体外小管形成实验观察石见穿氯仿及乙酸乙酯部位对HUVECs体外增殖、迁移、小管形成能力的影响。结果:3.125-25μg/ml的石见穿氯仿部位及乙酸乙酯部位作用于HUVEC 48h,增殖抑制率分别为17.36%-53.08%、9.53%-31.36%,而作用于人肺癌细胞A549增殖抑制率分别为7.10%-31.18%、2.24%-21.69%,两者相比有明显差异细胞毒作用(P=0.001)。3.125-25μg/ml的石见穿氯仿及乙酸乙酯部位作用HUVECs 12h后,迁移抑制率分别为5.39%-57.38%、1.92%-37.37%。3.75-25μg/ml石见穿氯仿部位作用24h时HUVEC小管形成数目减少且管腔不完整,相同浓度石见穿乙酸乙酯部位抑制作用较弱。结论:石见穿氯仿部位抑制内皮细胞增殖、迁移和小管形成作用明显优于乙酸乙酯部位,为石见穿发挥抗血管生成的主要作用部位。