Assessment of pulmonary infectious disease treatment with Mongolian medicine formulae based on data mining, network pharmacology and molecular docking

Objective: Pulmonary infectious diseases (PID) include viral pneumonia (VP) and pulmonary tuberculosis (PT). Mongolian medicine (MM) is an effective treatment option in China, however, the core group medicines (CGMs) in the treatment of PID and their underlying therapeutic mechanisms remain unclear. In this study, through the method of data mining, the CGMs of MM for the treatment of PID were excavated, and the possible mechanism of action of the CGMs in the treatment of PID was explored by using network pharmacology. Methods: First, 89 MM formulae for the treatment of pulmonary infectious diseases collected from Gan Lu Si Bu, Meng Yi Jin Kui, People''s Republic of China Ministry of Health Drug Standards (Mongolian Medicine Volume), Standard of Mongolian Medicine Preparations in Inner Mongolia (2007 Edition), and Standard of Mongolian Medicine Preparations in Inner Mongolia (2014 Edition). The CGMs of MM for PID were excavated through association rule analysis and cluster analysis. Then, the active ingredients and potential targets of the CGMs were obtained from TCMSP, TCMIP, BATMAN-TCM databases. PID targets information was collected from OMIM, GeneCards, and DrugBank databases. The possible targets of CGMs treatment for PID were obtained by intersection. The PPI network was constructed through the STRING database, and the topology analysis of the network was performed. Through the enrichment analysis of the intersection targets by R language, the main action pathways and related target proteins of CGMs in the treatment of PID were screened out. The results were verified by molecular docking. Results: A total of 89 formulae were included, involving 164 MM herbs. The efficacy of the drugs was mainly cough-suppressing and panting-calming herbs, and heat-clearing herbs. The nature and flavor were mainly bitter and cold. The CGMs of MM to treatment of PID was excavated as the classic famous formula Sanzi Decoction (Toosendan Fructus-Chebulae Fructus-Gardeniae Fructus). A total of 28 candidate components and 237 predicted targets of CGMs were collected, and 61 common targets with PID were obtained, including key compounds such as quercetin, kaempferol, β-sitosterol and stigmastero and key targets such as VEGFA, IL6, TP53, AKT1. KEGG enrichment analysis yielded AGE-RAGE signaling pathways, IL-17 signaling pathways, and TNF signaling pathways. Molecular docking results showed that the key targets were well matched with the potential active ingredients of CGMs. Conclusion: This study found that MM commonly used cough-suppressing and panting-calming herbs in combination with heat-clearing herbs to treat PID, and the CGMs for the treatment of PID is “Toosendan Fructus-Chebulae Fructus-Gardeniae Fructus”. CGMs mainly play a role in the treatment of PID by acting on VEGFA, IL6, TP53, AKT1 and other targets, regulating AGE-RAGE signaling pathways, IL-17 signaling pathways, and TNF signaling pathways.     

免疫抑制剂治疗类风湿关节炎的研究进展

类风湿关节炎（rheumatoid arthritis,RA）是风湿免疫科一种常见的全身性自身免疫性疾病。根据病情进展的不同程度,治疗方案亦不同,包括改善病情抗风湿药（disease-modifying anti-rheumatic drugs,DMARDs）、非甾体抗炎药（non-steroidal anti-inflammatory drugs,NSAIDs）、生物制剂、植物制剂等药物治疗以及辅助疗法,如针对性的锻炼、改善膳食、热敷冷敷等。目前的治疗方法对于RA的病情是有明显改善的,其中免疫抑制剂（immunosupressive agents,ISA）起到了不可忽视的作用,故从免疫抑制的角度论述,它能够有效控制病情进展,达到缓解疾病、提高生活质量的目标,得到了广泛的应用和发展。本文简述了选择性免疫抑制剂和非选择性免疫抑制剂治疗类风湿关节炎的作用机制以及临床应用,为免疫抑制剂治疗RA方面提供理论依据。     

柴胡舒肝丸治疗消化性溃疡随机平行对照研究

[目的]观察柴胡舒肝丸治疗消化性溃疡疗效。[方法]使用随机平行对照方法,将160例门诊患者按随机数字表法分为两组。对照组80例阿莫西林,1000mg/次,2次/d;奥美拉唑,20mg/d,2次/d;呋喃唑酮,100mg/次,3次/d。治疗组80例柴胡舒肝丸(茯苓100g,枳壳炒50g,豆蔻40g,白芍酒炒、甘草各50g,香附醋制75g,陈皮、桔梗、厚朴姜制、山楂炒、防风、六神曲炒各50g,柴胡75g,黄芩、薄荷50g,紫苏梗75g,木香25g,槟榔炒75g,三棱醋制、大黄酒炒、青皮炒、当归50g,姜半夏75g,乌药、莪术制50g),1丸/次,2次/d。两组均连续治疗7d为1疗程。观测临床症状、不良反应。连续治疗4疗程,判定疗效。[结果]治疗组治愈45例,显效20例,有效9例,无效6例,总有效率92.50%。对照组治愈38例,显效15例,有效16例,无效11例,总有效率86.25%。治疗组疗效优于对照组(P0.05)。[结论]柴胡舒肝丸治疗消化性溃疡效果显著,值得推广。     

HPLC-DAD法测定冠脉宁片中葛根素和丹酚酸B的量及其指纹图谱研究

目的 建立冠脉宁片中葛根素和丹酚酸B的量的测定方法,并对其指纹图谱进行初步研究。方法 采用HPLC-DAD法分别对葛根素和丹酚酸B进行量的测定,并采用此方法对冠脉宁片的指纹图谱进行初步研究。所使用的色谱柱为Phenomenex Luna C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇和0.5%甲酸溶液梯度洗脱,检测葛根素波长为250 nm,丹酚酸B为286 nm。结果 葛根素和丹酚酸B分别在0.002 523~0.504 6 mg/mL和0.002 657~0.425 2mg/mL的范围内呈良好的线性关系。平均回收率分别为99.53%(n=6,RSD=1.5%)和102.2%(n=6,RSD=1.4%)。56批样品的指纹图谱相似度计算结果为0.25~0.98。结论 所建立的检测方法简便、准确、快速,可用于冠脉宁片的质量控制。     

Comparison of a cytokine adsorbing column and an endotoxin absorbing column for the treatment of experimental endotoxemia

The present study compared the effects of direct hemoperfusion (DHP) using a cytokine absorbing (CTR) column with those using an endotoxin absorbing (PMX) column with endotoxin-induced shock in rats. Thirty-six rats were injected intravenously with endotoxin (15 mg/kg), and then allocated into one of the following three groups: control group, treated without CTR; PMX group, treated with a PMX column for 120 min; CTR group, treated with a CTR column for 120 min. The present study showed that CTR treatment decreased the mortality rate 8 h after endotoxin injection and inhibited inflammatory responses (cytokine, hemodynamics and acidosis) similar to PMX treatment.     

Present day controversies and consensus in curettage for giant cell tumor of bone

BackgroundThe ideal treatment for giant cell tumor of bone (GCTB) is still controversial. The purpose of this study was to evaluate whether curettage was successful in the treatment of GCTB. Intralesional curettage with adjuvant therapies, such as high-speed burring, polymethylmethacrylate, phenol, ethanol, and liquid nitrogen, may be used to reduce the local recurrence rate. However, there is no consensus on the optimal use of curettage, along with fillers and adjuvants, to limit the recurrence rate.MethodsWe performed a systematic review of articles using the terms long bones, GCTB, and treatment. Case reports, reviews, opinion articles, or technique notes were excluded based on the abstract. Twenty-six articles included in this review were then studied to establish the index in suggesting the surgical treatment of GCTB.ResultsThe patient’s gender, their age, the Campanacci grade of their tumor, and the type of surgery they had were not significantly associated with the local recurrence rate. Local recurrences seemed to be associated with the site of the tumor, occurring more frequently in the proximal femur or distal radius. A pathological fracture was not a contraindication for intralesional curettage. Treatment with denosumab did not decrease the local recurrence rate in patients who had been treated with curettage.ConclusionThe current literature seems to suggest that the ideal treatment for GCTB is to remove the tumor while preserving as much of the joint as possible. Local recurrent tumors can be treated with curettage to keep the re-recurrence rate within an acceptable limit. The choice for how to treat GCTB in the proximal femur or distal radius requires special attention.     

独活寄生汤配合温针灸治疗风寒湿痹型膝骨关节炎患者的疗效及对血清疼痛介质、炎症介质、白三烯的影响

目的:观察独活寄生汤配合温针灸治疗风寒湿痹型膝骨关节炎(KOA)膝关节疗效及对血清疼痛介质、炎症介质、白三烯(LT)的影响。方法:将94例风寒湿痹型KOA患者随机分为对照组(30例),独活寄生汤组(34例)及联合组(30例),对照组给与硫酸氨基葡萄糖胶囊0.5 g/次,3次/d口服;独活寄生汤组给予独活寄生汤150 m L,2次/d口服;联合组在独活寄生汤组治疗基础上给予温针灸治疗,疗程均为4周;观察入组患者治疗前后膝关节视觉模拟评分(VAS),自制膝关节肿胀评分及总有效率变化;酶联免疫吸附法(ELISA)检测各组患者血清中疼痛介质[前列腺素E2(PGE2),P物质(SP),多巴胺(DA),五羟色胺(5-HT)],炎症介质[肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β)]及白三烯B4,白三烯C4,白三烯D4(LTB4,LTC4,LTD4)的表达。结果:治疗后联合组VAS,膝关节肿胀评分及对KOA疼痛疗效明显高于对照组和独活寄生汤组(P0.05);与对照组治疗后相比,独活寄生汤组及联合组患者血清PGE2,SP,DA,5-HT,TNF-α,IL-1β,LTB4,LTC4,LTD4含量降低(P0.05),与独活寄生汤组治疗后相比,联合组患者血清PGE2,SP,DA,5-HT,TNF-α,IL-1β,LTB4,LTC4,LTD4含量降低趋势更加明显(P0.05)。结论:独活寄生汤配合温针灸可有效治疗风寒湿痹型KOA患者膝关节肿痛,其机制可能与降低LTB4,LTC4,LTD4及炎症介质含量有关。     

加味龟鹿养骨汤联合甲状旁腺激素对绝经后骨质疏松患者身体成分的影响

目的:观察加味龟鹿养骨汤联合甲状旁腺激素对绝经后骨质疏松患者身体成分的影响。方法:选取2013年1月至2015年12月内蒙古医科大学附属医院收治住院的绝经后骨质疏松患者54例,记录年龄、BMI、病情等临床资料,根据随机数字表法分为对照组和观察组,每组27例,2组患者均采用甲状旁腺激素治疗,观察组在对照组的治疗基础上加用中药汤剂-加味龟鹿养骨汤,观察中医证候疗效、血清25-羟维生素D(25-OH-VD)水平、骨钙素(BGP)及β-Ⅰ型胶原羧基端肽(CTX)、骨密度等指标,评价加味龟鹿养骨汤对PMOP患者的作用。结果:治疗后观察组有效治愈率(96.29%)明显高于对照组(85.18%),差异有统计学意义(P0.05);在治疗后,L1-4、股骨颈及股骨大转子的BMD差异有统计学意义(P0.05),观察组与对照组比较,差异有统计学意义(P0.01);治疗后CTX及BGP显著减少,25-OH-VD水平增加,而观察组比对照组更为明显(P0.05);在12个月的随访中,观察组没有新的骨折发生,对照组2例患者出现新的骨折,包括髋部骨折1例,桡骨远端骨折1例。结论:加味承气汤对急性重症胰腺炎患者有一定的临床疗效,可调节患者25-OH-VD、BGP、CTX水平,增加患者骨密度,有效治疗绝经后骨质疏松症患者。     

中医序贯疗法治疗溃疡性结肠炎的疗效分析

目的：探讨中医序贯疗法治疗溃疡性结肠炎的临床疗效。方法：选取2009年1月～2010年5月在我院就诊的100例溃疡性结肠炎患者,将其随机分为对照组与治疗组,每组各50例。治疗组先依据湿热内蕴证进行清肠化湿,病情缓解后依据脾胃虚弱程度进行清肠扶正中药序贯疗法治疗;对照组采用美沙拉嗪进行治疗,对比其临床疗效。结果：经过6个月的治疗后,治疗组复发率为6%（3/50）,对照组复发率为28%（14/50）,治疗组的疗效优于对照组,差异有统计学意义（P〈0.05）。治疗组不良反应发生率为2%（1/50）,对照组不良反应发生率为8%（4/50）,治疗组不良反应发生率低于对照组,差异有统计学意义（P〈0.05）。结论：中医序贯疗法治疗溃疡性结肠炎,疗效显著,值得临床应用推广。     

蒙古族人群原发性高血压与AngII受体1基因多态性相关性研究

目的探讨血管紧张素II受体1（AT1R）基因多态性与内蒙地区蒙古族人群原发性高血压的关系。方法选取内蒙古地区蒙古族原发性高血压患者（EH组）以及正常血压对照组（C组）,EH组纳入研究对象200例,C组纳入232例。应用PCR技术检测EH组和C组的AT1R基因A1166C点多态性。结果所有纳入研究的内蒙地区蒙古族群体中AT1R基因A/C多态频率分布符合Hardy-Weinberg平衡;EH组和C组基因型频率分布差异有统计学意义（P=0.014）,EH组人群AC＋CC基因型比例显著高于C组。结论 AT1R基因A1166C多态性同内蒙地区蒙古族群体高血压病发病相关。