布地奈德/福莫特罗联合氨溴索雾化吸入治疗儿童中度持续性哮喘疗效分析

目的观察布地奈德/福莫特罗联合氨溴索治疗儿童中度持续性哮喘的疗效。方法将80例中度哮喘患儿予布地奈德/福莫特罗粉剂联合氨溴索雾化吸入治疗3个月,治疗前后分别进行临床症状评分,肺功能检测并观察不良反应。结果 80例患儿治疗后临床症状和肺功能均较治疗前显著改善(P<0.05),不良反应为声嘶、咽部不适。结论布地奈德/福莫特罗联合氨溴素治疗儿童中度持续性哮喘疗效和安全性较好。     

癌源exosomes的制备及其对胃癌细胞生长的作用

目的:分离癌细胞源exosomes,对其超微结构进行鉴定,探讨exosomes对胃癌细胞增值活性的影响。方法:采用超速梯度离心法从MGC 803胃癌细胞培养上清液中提取exosomes,通过透射电镜技术对其超微结构进行鉴定,并利用MTT法探讨exosomes对胃癌细胞增殖活性的作用。结果:胃癌MGC 803细胞来源的exosomess具有特征性的盘状结构,由双层膜构成,直径约50~130 nm。Exosomes以剂量依赖性的方式促进MGC 803细胞的增殖。结论:癌细胞来源的exosomes能促进胃癌细胞增殖,其表面所负载肿瘤抗原可能在肿瘤细胞增殖过程中起重要的信息传递作用。     

老年高血压患者胰岛素抵抗与左室结构改变的关系

目的探讨老年原发性高血压患者胰岛素抵抗（IR）在高血压发生、发展过程中对左室结构的影响。方法72例高血压患肯依照左室质量指数（LVMI）和相对室壁厚度（RWT）分为左室正常构型组（34例）、向心性重构组（18例）、向心性肥厚组（11例）、离心性肥厚组（9例），并行口服葡萄糖耐量试验（OGTT）加同步胰岛素释放试验，计算胰岛素敏感性指数（ISI）、血糖曲线下面积（AG）、胰岛素曲线下面积（AI）、空腹血胰岛素/空腹血糖（FSI／FSG）比值、AI／AG比值。设健康对照组35例。应用单元和多元回归分析观察RWT和LVMI与各胰岛素敏感性指标的关系。结果高血压组的RWT与FSI、FSG无关（P〉0．05），与AI、AG呈正相关（r值分别为0．160、0．227，P〈0．05），与ISI呈负相关（r值为=0．266，P〈0．01）；LVMI与各胰岛素敏感性指标无相关性（P〉0．05）。逐步回归分析显示RWT与ISI呈独立相关（r^2=0．071、P〈0．05）。结论高胰岛素血症（HIS）及IR存在于老年高血压患者各种左室几何构型中，其中与向心性重构关系最密切，与IR相关的HIS是参与和促进左心室向心性重构的重要影响因素。     

How to determine post-RCHOP therapy for risk-tailored adult patients with diffuse large B-cell lymphoma,addition of maintenance rituximab or observation: multicenter experience

Background  

In international prognostic index (IPI) risk-tailored adult patients with diffuse large B-cell lymphoma (DLBCL), it is still unclear whether the addition of maintenance rituximab (MR) improves progression-free (PFS) and overall survival (OS), after RCHOP (rituximab, cyclophosphamide, doxorubicin, vincristine and prednisone) therapy.     

Mesenchymal stem cells: A revolution in therapeutic strategies of age-related diseases

The great evolutionary biologist Theodosius Dobzhansky once said: “Nothing in biology makes sense except in the light of evolution”. Aging is a complex biological phenomenon and the factors governing the process of aging and age-related diseases are only beginning to be understood, oxidative stress, telomere shortening in DNA components and genetic changes were shown to be the mainly regulating mechanisms during the recent decades. Although a considerable amount of both animal and clinical data that demonstrate the extensive and safe use of mesenchymal stromal cells (MSCs) is available, the precise summarization and identification of MSCs in age-related diseases remains a challenge. Along this line, this review discussed several typical age-related diseases for which MSCs have been proved to confer protection and put forward a hypothesis for the association among MSCs and age-related diseases from an evolutionary perspective. Above all, we hope further and more research efforts could be aroused to elucidate the role and mechanisms that MSCs involved in the age-related diseases.     

呼和浩特市地区大学生艾滋病知识教育效果分析

目的了解大学生艾滋病知识的掌握程度,评价已开展艾滋病健康教育干预措施的效果。方法2007年7月,在呼和浩特市几所大学院校对部分学生进行问卷调查。结果宣传教育后,大学生对艾滋病的相关知识的认识都有了提高（P〈O．01）;对艾滋病持有的态度有了改善（p〈0．01）,不同民族的学生在掌握艾滋病相关知识的程度没有差异（P〉O．05）;女生在对艾滋病的相关知识认识高于男生,但对艾滋病的传播途径男生更高,低年级的学生艾滋病的相关知识较新生和高年级学生正确率高,均有统计学意义（P〈O．01）。结论该项目在艾滋病的知识宣传上起到了重要的作用,从另一个角度说明该项目的有效性和可行性,且实施的内容和方法较简单,可以建议各大学院校推广实施。     

神经生长因子功能和应用的研究进展

神经生长因子(NGF)是发现最早,研究最透彻的神经营养因子。本研究主要就NGF的功能、生物学活性检测,重组NGF的研究进展,NGF的临床应用研究进展等方面加以综述,并对NGF的未来发展做一展望。     

P2X3受体在糖尿病神经病理性疼痛模型中的作用

目的观察P2X3受体在糖尿病神经病理性疼痛大鼠模型中的作用和意义。方法将40只SD大鼠随机分为造模组（n=30）和对照组（n=10）。按照60 mg/kg一次性腹腔注射链脲佐菌素（STZ）建立糖尿病大鼠模型。于STZ注射前和注射后第7、14、28天分别测定大鼠血糖、体重以及机械性痛阈的变化。造模组又均分为造模1周组、造模2周组、造模4周组三个亚组。在造模前及造模后1、2、4周利用von Frey hairs法测定大鼠机械痛阈,并通过Real-time法测定每个时间点大鼠脊髓背根神经节（DRG）P2X3受体的表达水平。结果肉眼观察糖尿病大鼠毛色暗淡,尾静脉采血点愈合缓慢,每日饮水,饮食,尿量显著增加,表现符合糖尿病大鼠的临床特征。各模型组血糖浓度均〉16.7 mmol/L,各模型组与对照组比较,差异有统计学意义（P〈0.01）。糖尿病大鼠体重较对照组同期大鼠增长缓慢,差异有统计学意义（P〈0.01）。造模组机械痛阈在造模1周后明显下降（P〈0.01）,并持续至4周（P〈0.01）;在造模1周后脊髓DRG内P2X3受体表达水平出现显著上调（P〈0.01）。大鼠P2X3受体表达水平变化与机械痛阈的下降存在相关性。结论糖尿病早期大鼠脊髓DRG P2X3受体表达即出现上调,并与疼痛相关,说明P2X3受体可能在糖尿病大鼠周围神经疼痛的发生及痛敏的维持阶段起重要作用。     

Influence of citrate on phase transformation and photoluminescence properties in LaPO4 and LaPO4:Eu

The hexagonal and monoclinic phase LaPO₄ and LaPO₄:Eu nanostructures have been controllably synthesized by a citrate-induced hydrothermal process at 100 °C. The crystal growth of LaPO₄ nanostructures was investigated, and the phase transformation of nanostructured LaPO₄ was systematically studied by varying the citrate concentration, pH value and reaction temperature. When 0.8 mmol of citrate was added into the reaction system, the hexagonal phase LaPO₄ transformed into the monoclinic phase. High concentrations of citrate would lead to the formation of hexagonal phase LaPO₄. The photoluminescence properties of the monoclinic phase LaPO₄:Eu prepared using a citrate-induced process demonstrate that the electric dipole transition (⁵D₀ → ⁷F₂) is stronger than the magnetic dipole transition (⁵D₀ → ⁷F₁), which indicated that Eu³⁺ is in a site with no inversion center. The strongest emission peak of hexagonal phase LaPO₄:Eu comes from ⁵D₀ → ⁷F₁. Furthermore, the citrate-induced hexagonal phase LaPO₄:Eu has a stronger emission intensity than the hexagonal phase LaPO₄:Eu prepared not using a citrate-induced process.

The hexagonal and monoclinic phase LaPO₄:Eu nanostructures have been controllably synthesized using a citrate-induced hydrothermal process at 100 °C.