气-质联用法测定鱼腥草注射液中甲基正壬酮的含量

目的:建立用气-质联用仪,以正十五烷为内标,采用选择监测离子法测定鱼腥草注射液中甲基正壬酮含量的方法。方法:色谱柱DB-5MS(30 mm×0.32 mm×0.25μm)石英毛细管色谱柱,样品用正己烷萃取,采用选择离子监测法定量。结果:甲基正壬酮在1.0～10 mg.L-1范围内线性良好(r=0.992 7);回收率95.30%～98.69%,检测限在1.0ng.mL-1。结论:本方法简便、快速、灵敏度高、结果准确可靠。     

三七总皂甙对缺氧缺血性脑损伤新生鼠内源性神经干细胞增殖分化的影响

目的:观察三七总皂甙对缺氧缺血性脑损伤(HIBD)新生鼠内源性神经干细胞增殖分化的影响,探讨其促进神经发生的作用。方法 :将72只7日龄新生SD乳鼠随机分为假手术组、HIBD组及HIBD的三七总皂甙治疗组,用改良的Rice-Vannucci法制作HIBD模型。注射三七总皂甙干预治疗,用5一溴脱氧尿苷(BrdU)标记处于增殖状态的神经干细胞,免疫单标及双标免疫荧光技术观察三七总皂甙对HIBD海马区BrdU免疫活性及BrdU/NSE(Neuron specific enolase,NSE)、BrdU/GFAP(Glial fibrillary acidic protein,GFAP)双标免疫活性的影响,并计数分析。结果:HIBD的海马区存在BrdU阳性细胞分布;应用三七总皂甙的HIBD海马区BrdU阳性细胞数及双标阳性细胞明显增多(P<0.01)。结论:三七总皂甙能诱导HIBD新生鼠内源性神经干细胞增殖、分化,提示其具有促进神经发生的能力。     

颅脑损伤患者血清NSE的含量变化及临床意义

目的动态监测颅脑损伤患者血清神经元特异性烯醇化酶(NSE)的含量变化,并探讨NSE与颅脑损伤程度及其预后之间的关系。方法用酶联免疫测定法检测86例颅脑损伤患者的血清NSE含量,并分析其与颅脑损伤严重程度及患者预后的关系。结果颅脑损伤患者血清NSE水平明显高于对照组(P<0.05);重度颅脑损伤患者血清NSE水平又明显高于中、轻度颅脑损伤者(P<0.05);重度颅脑损伤预后不良者的血清NSE水平则明显高于预后良好者(P<0.05)。结论血清NSE水平与脑损害的严重程度呈正相关,可作为评估颅脑损伤预后的重要指标。     

神经内镜下治疗梗阻性脑积水

目的 探讨对梗阻性脑积水患采用神经内镜下进行第三脑室底和(或)透明隔造瘘的手术方法进行治疗，分析手术成功与失败的原因。方法 对10例不同原因造成的梗阻性脑积水患进行10次神经内镜下第三脑室底造瘘或，和透明隔造瘘术。结果 随诊1—12月，平均6月显效8例有效1例无效1例(改V—P分流术)。手术并发症：1例轻度颅内感染、1例轻度脑室内出血，无死亡病例。结论 神经内镜下第三脑室底造瘘或，和透明隔造瘘手术治疗梗阻性脑积水是一种完全有效的手术方法。但为了提高手术疗效应有良好的手术器械，严格掌握手术适应症并能熟练进行手术操作。     

A fluorescent biosensor for cardiac biomarker myoglobin detection based on carbon dots and deoxyribonuclease I-aided target recycling signal amplification

A sensitive biosensor using carbon dots and deoxyribonuclease I-aided target recycling signal amplification has been developed to detect myoglobin (MB), which is an important cardiac biomarker and plays a major role in the diagnosis of acute myocardial infarction (AMI). Here, in the absence of MB, the MB aptamer (Ap) is absorbed on the surface of carbon dots (CDs) through π–π stacking interactions, resulting in quenching of the fluorescent label by forming CD–aptamer complexes. Upon adding MB, the Ap sequences could be specifically recognized by MB, leading to the recovery of quenched fluorescence. Thus, quantitative evaluation of MB concentration has been achieved in a broad range from 50 pg mL⁻¹ to 100 ng mL⁻¹, and the detection limit is as low as 20 pg mL⁻¹. This strategy is capable of specific and sensitive detection of MB in human serum, urine, and saliva and can be used for the diagnosis of AMI in the future.

A sensitive biosensor has been developed to detect myoglobin (MB), which is an important cardiac biomarker and plays a major role in the diagnosis of acute myocardial infarction (AMI).     

多层螺旋CT与核磁共振成像在细微及隐匿性骨折中的诊断价值

目的：探讨多层螺旋CT（MSCT）与核磁共振成像（MRI）检查在诊断细微及隐匿性骨折中的阳性率和特异性,对比分析两种检查方法诊断细微及隐匿性骨折的价值。方法回顾性分析湖北医药学院附属东方医院诊断的细微及隐匿性骨折150例,分析 M SC T、M RI、X线检测骨折部位,计算总检出数及阳性检出率,对比分析MSCT、MRI诊断细微及隐匿性骨折的优势和特异性。结果 MSCT诊断骨折125例,阳性检出率83．33％。同时经MRI检查,T1WI检出骨折141例,阳性检出率94．00％,T2WI骨折检出率100．00％。MSCT与MRI对细微及隐匿性骨折阳性检出率差异有统计学意义（P＜0．05）。结论 MRI在骨挫伤中显影率高,不同扫描参数可对比得出骨折数据,尤其适用于隐匿性骨折及合并血肿、脂肪覆盖等骨折情况复杂的患者。     

Clinical Significance of Heart Chronotropic Incompetence in Patients With Coronary Heart Disease

Objectives To investigate the relationship between the chronotropic incompetence and angiographic severity of coronary artery disease, and the clinical value of inappropriate chronotropic responses in exercise. Methods Coronary angiography was performed in 130 patients suspected or diagnosed as coronary heart disease （ CHD）, and angiographic severity of coronary artery disease was quantitated by Duke score and Gensini score. The patients were divided into 4 groups ： non-CHD group （39 cases）, CHD group with only one coronary artery involved （ CHD1, 30 cases）, CHD group with two coronary arteries involved （ CHD2, 31 cases） and CHD group with three coronary arteries involved （CHD3 group, 30 cases ）. A month before coronary angiography, symptom-limited bicycle ergometor exercise had been accomplished, the chronotropic response had been measured and expressed as ratio of heart rate reserve （HRR） and the maximal age-predicted heart rate achieved （rHR）. Results Analysis of variance showed that rHR and HRR were much significantly lower （all P 〈0. 01 ） in CHD2 group （rHR 0. 793 ±0. 078, HRR 0. 626±0. 110） and CHD3 group （ rHR 0. 775 ± 0. 065, HRR 0. 586 ± 0. 125 ） than that in non-CHD group （ rHR 0. 888 ± 0. 062, HRR 0. 798 ±0. 105）and CHD1 group（rHR 0. 857 ±0. 084, HRR 0. 735 ±0. 146）. rHR was similar both between non-CHD group and CHD1 group（ P 〉 0. 05 ） and between CHD2 group and CHD3 group （ P 〉 0. 05 ）. HRR has no difference between CHD2 group and CHD3 group （ P 〉 0. 05 ）, but was significantly different between non-CHD group and CHD1 group （P 〈0. 05 ）. There was a significantly negative correlation between rHR, HRR and Duke score （r = -0. 554, - 0. 578, respectively, all P 〈0. 01 ）, Gensini score （ r = -0. 453, -0. 467 ,respectively, all P 〈0. 01 ）. CHD proportion reached 75% in patients who had positive rHR （ or HRR） and non-ST depression. Diagnostic value [ sensitivity 0. 868 （P 〈0. 01 ）, 0. 846（P 〈0. 01 ?     

丙型肝炎病毒对白细胞介素32表达影响的研究

目的 探讨丙型肝炎病毒（HCV）在体内外对白细胞介素32（IL-32）表达的影响。方法 荧光定量PCR和免疫印迹法检测Huh7.5.1细胞中IL-32 mRNA和蛋白的表达水平,酶联免疫吸附试验（ELISA）检测IL-32的血清含量。结果 HCV感染的Huh7.5.1细胞IL-32 mRNA和蛋白的表达水平较对照细胞高,HCV患者IL-32血清含量明显高于健康对照（P〈0.05）。结论 HCV能够在体内外促进IL-32的合成和分泌。     

糖调节受损人群下肢血管病变患病率及相关因素分析

目的:探讨糖调节受损(IGR)人群下肢血管病变(PVD)的患病率及相关影响因素。方法:以681例IGR人群为研究对象,收集相关临床资料、体格检查、生化检查结果及多普勒超声血流仪测定踝臂指数(ABI),以ABI≤0.9诊断有PVD,分为PVD组和非PVD组,并对两组进行分析。结果:681例IGR人群中检出ABI≤0.9者49例,患病率为7.2%。两组年龄、收缩压、低密度脂蛋白胆固醇、甘油三酯、空腹胰岛素、胰岛素抵抗指数、吸烟率比较差异均有统计学意义(P<0.05),Logistic回归分析发现,年龄、收缩压、低密度脂蛋白胆固醇是糖调节受损下肢血管病变的独立危险因素。结论:下肢血管病变在糖尿病前期IGR阶段已发生,且发生率较高,增龄、吸烟、高血压、血脂紊乱及胰岛素抵抗是下肢血管病变的重要危险因素。     

高效液相色谱法测定乌蕨中牡荆苷的含量

目的建立测定乌蕨中牡荆苷含量的高效液相色谱方法。方法采用DIONEX-C18柱(4.6mm×250mm,5μm),以乙腈∶甲醇∶0.2%冰醋酸水溶液(23∶77)为流动相,流速1.0mL/min,柱温30℃,检测波长270nm。结果牡荆苷在0.009-0.180mg/mL浓度范围内有良好线性关系(r=0.9995),平均加样回收率为98.0%,RSD小于2.0%。结论本方法简便、快速、准确、重复性好,可用于乌蕨中牡荆苷的含量测定。