β受体信号系统对心力衰竭中心肌细胞凋亡的作用

在胎儿期和生后发育早期心肌细胞的凋亡决定着成人心肌细胞数量。然而,成人心肌细胞明显有限的再生能力提示凋亡导致的心肌细胞的持续丢失可能促成了进展的心力衰竭。现着重阐述β受体信号系统在介导心肌细胞凋亡中的作用。     

2009年我院眼科门诊电子处方存在的问题与分析

目的：分析我院眼科门诊电子处方存在的问题,探讨改善眼科门诊处方质量的措施,提高处方质量。方法：随机抽取我院2009年眼科门诊电子处方2400张,分析处方不合理情况,统计不合理处方比例。结果：不合理处方84张,占所抽取处方的3.50%。其中年龄与诊断不符的4张,占不合理处方的4.76%;用法用量不符合要求的32张,占不合理处方的38.10%;诊断与用药不符的7张,占不合理处方的8.30%;用药周期过长的24张,占不合理处方的28.50%;无医师修改印章与日期的6张,占不合理处方的7.14%;药物配伍禁忌的2张,占不合理处方的2.38%;重复用药的10张,占不合理处方的11.90%。结论：电子处方的实行大大降低了不合理处方的发生,但仍应进一步对门诊电子处方系统功能进行完善并加强药师审核处方的水平与力度,规范医生开具处方,提高我院门诊处方的质量。     

眼科门诊药房退药处方情况分析及解决方案

目的：分析哈尔滨医科大学附属第一医院眼科门诊的退药情况,提高处方质量。方法：根据我院2009年1—12月458份眼科门诊患者的退药处方,统计分析退药原因。结果：458份退药处方中,因不良反应而要求退药的382例,占总数83.40%,其他各类原因共占16.60%。结论：应加强眼科合理用药指导,减少不良反应发生,进一步减少退药的发生。     

BMP-2/PLGA Delayed-Release Microspheres Composite Graft,Selection of Bone Particulate Diameters,and Prevention of Aseptic Inflammation for Bone Tissue Engineering

Autogenous bone grafts are widely used in the repair of bone defects. Growth factors such as bone morphogenetic protein 2 (BMP-2) can induce bone regeneration and enhance bone growth. The combination of an autogenous bone graft and BMP-2 may provide a better osteogenic effect than either treatment alone, but BMP-2 is easily inactivated in body fluid. The objective of this study was to develop a technique that can better preserve the in vivo activity of BMP-2 incorporated in bone grafts. In this study, we first prepared BMP-2/poly(lactic-co-glycolic acid) (PLGA) delayed-release microspheres, and then combined collagen, the delayed-release microspheres, and rat autologous bone particulates to form four groups of composite grafts with different combinations: collagen in group A; collagen combined with bone particulates in group B; collagen combined with BMP-2/PLGA delayed-release microspheres in group C; and collagen combined with both bone particulates and BMP-2/PLGA delayed-release microspheres in group D. The four groups of composite grafts were implanted into the gluteus maximus pockets in rats. The ectopic osteogenesis and ALP level in group D (experimental group) were compared with those in groups A, B, and C (control groups) to study whether it had higher osteogenic capability. Results showed that the composite graft design increased the utility of BMP-2 and reduced the required dose of BMP-2 and volume of autologous bone. The selection of bone particulate diameter had an impact on the osteogenetic potential of bone grafts. Collagen prevented the occurrence of aseptic inflammation and improved the osteoinductivity of BMP-2. These results showed that this composite graft design is effective and feasible for use in bone repair.     

日本医科大学附属病院医院药学开展的介绍及对我国的启发

目的:了解日本医院药学现状,为我国医院药学模式的进一步完善提供参考。方法:介绍日本医科大学附属病院医院药学开展的情况及日本医院药学在近几十年的发展变化。结果与结论:我国医院药学与日本相比,水平及完善程度还具有一定差距,因而通过明确药师的责任与义务等来完善我国的医院药学制度尤为重要。     

注射用氨曲南与3种输液的配伍稳定性考察

目的:考察注射用氨曲南与果糖注射液、转化糖注射液和木糖醇注射液配伍后的稳定性,为糖尿病患者输液时溶媒的选择提供参考。方法:观察注射用氨曲南与3种输液配伍后在室温下6h内氨曲南的含量、微粒数量、溶液pH值及其他物理变化。结果:配伍液在6h内含量、pH值、微粒检查均符合要求。结论:注射用氨曲南在果糖注射液、转化糖注射液和木糖醇注射液中配伍稳定。     

后腹腔镜肾盂上段输尿管切开取石术的临床分析

目的 探讨后腹腔镜肾盂或输尿管切开取石治疗肾或输尿管上段结石的适应证,手术技巧和临床价值.方法 回顾分析采用后腹腔镜治疗肾盂结石1例,输尿管结石4例,输尿管结石位于L3～L5平面,5例患者术前行ESWL治疗失败,结石直径1.5～2.0 cm,平均1.8 cm.结果 5例患者均手术成功,手术时间110～175 min,平均145 min:术中出血量30～50 mL,平均35 mL;术后无漏尿;患者术后3～5 d拔出腹膜后引流管,术后住院5～7d,平均5.8d.结论 后腹腔镜肾盂输尿管切开取石术可作为体外冲击波碎石治疗失败的补救措施,对于不适合经皮肾镜或输尿管镜治疗的结石可作为首选治疗方法,具有取石取净率高,损伤小,安全,可靠等优点.     

A Novel Mutation in CACNA1S Gene Associated with Hypokalemic Periodic Paralysis Which has a Gender Difference in the Penetrance

Hypokalemic periodic paralysis (HypoPP) is an autosomal dominant disorder characterized by periodic attacks of muscle weakness associated with a decrease in the serum potassium level. Several mutations in the skeletal muscle calcium channel α-subunit gene CACNA1S have been documented to be causative for HypoPP, but mutations in other genes have also been implicated in HypoPP. To further reveal the genetic causes of HypoPP, we genotyped members of a five-generational Chinese family with HypoPP patients and identified a novel His916Gln mutation in all male HypoPP patients of the family. Clinical analysis demonstrated that the penetrance of the mutation was complete in male carriers, but we did not find evident clinical features in female carriers. This study expanded the spectrum of CACNA1S mutations associated with HypoPP and demonstrated a gender difference in the penetrance of the disease.     

骨髓来源的间充质干细胞对胃癌细胞SGC-7901增殖的影响

目的 研究骨髓来源的间充质干细胞对胃癌细胞SGC-7901增殖的影响,为胃癌的治疗与研究提供新的思路及方法.方法 原代培养骨髓来源的间充质干细胞,传代培养胃癌细胞SGC-7901,间充质干细胞及5-Fu对胃癌细胞SGC-7901增殖能力的影响采用MTT比色法.结果 24h内,与MCM组及DMEM相比,MSC组胃癌细胞S...     

HSP90与垂体腺瘤侵袭性关系的研究

目的 分析HSP90在垂体腺瘤中的表达,探讨HSP90与垂体腺瘤侵袭性之间的关系,研究以HSP90的表达水平作为判断垂体腺瘤侵袭性和预后指标的可能性.方法 取经手术切除且病理诊断为垂体腺瘤、具有完整内分泌资料的患者60例,根据Hardy-Wilson及Knosp分级、分期标准,结合病理活检判断其肿瘤侵袭性,将实验患者分...