红花黄色素对佐剂型关节炎大鼠的抗炎作用研究

目的 观察红花黄色素对佐剂型关节炎大鼠的抗炎作用并探讨其机制。方法 用弗氏完全佐剂建立佐剂型关节炎大鼠模型,分为正常组、模型组、红花黄色素100,50,25 mg·kg^-1组,每组10只。排水法检测大鼠足趾肿胀度,Elisa法检测炎症因子IL-1β及TNF-α的水平,western blot法检测滑膜组织中IL-1β和TNF-α蛋白的表达。结果 与模型组相比,红花黄色素各组能显著降低佐剂型关节炎大鼠的足趾肿胀度（p<0.01或P<0.05）,降低血清中IL-1β及TNF-α含量水平（p<0.01或p<0.05）,还能降低滑膜组织中IL-1β及TNF-α蛋白的表达（p<0.01或p<0.05）。结论 红花黄色素能显著缓解佐剂型关节炎大鼠的关节炎症,其机制与下调炎症因子IL-1β及TNF-α的表达有关。     

二陈汤加减治疗小儿毛细支气管炎临床疗效观察

目的:研究二陈汤加减(橘红痰咳液)对毛细支气管炎患儿的痰量、咳嗽、咳痰困难、哮鸣音、肺功能的临床疗效。方法:选择200例出生后24个月之内毛细支气管炎儿童患者,根据就诊先后顺序,采用随机数表法分为对照组和治疗组(二陈汤加减组)各100例。两组患儿均给予常规对症治疗,雾化吸入沙丁胺醇。观察组同时给予二陈汤加减(橘红痰咳液)治疗,2～5 mL/次,3次/d,口服。两组疗程均为15 d。观察患儿治疗15 d后的呼吸道症状,记录咳嗽、痰量、咳痰难易程度、肺部哮鸣音等临床表现的评分、疗效及咳嗽、哮鸣音等症状持续时间。治疗15 d后进行肺功能水平检测,记录呼吸频率(RR),每1 kg体质量潮气量(VT/kg),吸呼比(TI/TE),呼气达峰时间(TPTEF),达峰时间比(VPTEF/TE),呼气峰流速(PEF),呼气达峰容积(VPTEF),达峰容积比(PFV),呼出75%潮气量时的呼气流速/呼气峰流速(25/PF),潮气呼气中期流速/潮气吸气中期流速(ME/MI),呼吸系统阻力(Rrs),每1 kg体质量功能残气量(FRC/kg),每1 kg体质量顺应性(Crs/kg)水平。结果:在患儿痰量、咳嗽、咳痰难度、哮鸣音面,两组均得到明显的减轻;气道功能方面,两组均得到明显的提升。在治疗15 d后,治疗组在改善患儿痰量、咳嗽、咳痰困难、哮鸣音方面的疗效均优于对照组(P 0. 05)。治疗组明显缩短患儿咳嗽、哮鸣音持续时间。治疗15 d后,治疗组PFV,25/PF,ME/MI,Crs/kg,Rrs,FRC/kg与对照组比较有明显改善效果(P 0. 05)。结论:在常规治疗基础上,服用二陈汤加减(橘红痰咳液)治疗小儿毛细支气管炎能明显缓解咳痰量、咳痰难度、咳嗽、哮鸣音等临床症状、提高肺功能,提高治疗的有效性,未发生明显不良反应,值得临床推广。     

针刺治疗儿童脑源性视力损伤的疗效观察

目的分析针刺治疗3~10岁儿童脑源性视力损伤(CVI)的可行性和有效性。方法选取符合纳入标准的CVI患儿60例(120只眼),随机分为对照组30例(60只眼)和治疗组30例(60只眼)。对照组采用眼科常规视刺激治疗,治疗组在对照组基础上联合针刺治疗。按照年龄,分为低年龄组(3~5岁)和高年龄组(6~10岁)。治疗3个月,分别于治疗前和治疗后检查2组患儿的最佳矫正视力(BCVA)并记录数据。结果(1)BCVA:治疗后,2组比较,差异有统计学意义(t=6.194,P=0.000)。治疗前后比较,t_治疗组=22.826,t_对照组=8.226,均P=0.000,差异均有统计学意义。3~5岁患儿:治疗后2组比较,差异有统计学意义(t=4.061,P=0.000)。治疗前后比较,t_治疗组=21.899,t_对照组=7.336,均P=0.000,差异均有统计学意义。6~10岁患儿:治疗后2组比较,差异有统计学意义(t=4.497,P=0.000)。治疗前后比较,t_治疗组=14.346,t_对照组=5.568,均P=0.000,差异均有统计学意义。(2)临床疗效:2组CVI患儿总有效率比较,对照组10.00%,治疗组63.33%,差异具有统计学意义(Х²=34.486,P=0.000)。3~5岁患儿:对照组总有效率为21.43%,治疗组85.71%,差异具有统计学意义(Х²=20.749,P=0.000)。6~10岁患儿:对照组总有效率为0.00%,治疗组为43.75%,差异具有统计学意义(Х²=15.451,P=0.000)。结论针刺联合眼科常规视刺激可作为临床有效且安全性较高的中西医结合治疗方案,能更好地改善儿童CVI最佳矫正视力等指标,优于单纯常规视刺激治疗。     

中药灌肠治疗小儿发热的疗效及其对血清C反应蛋白的影响

目的:讨论中药灌肠治疗小儿发热的效果,并观察其对血清C反应蛋白(CRP)水平的影响,为临床治疗提供参考。方法:选取200例发热患儿,按入院单双号分为试验组与对照组各100例。两组患儿均给予抗感染、雾化及对症支持治疗;患儿体温>38.5 ℃时,对照组给予布洛芬混悬液退热,试验组给予中药退热合剂灌肠,观察并比较两组患儿疗效、体温恢复正常时间及血清CRP水平。结果:试验组患儿的体温恢复正常时间短于对照组,治疗后血清CRP水平明显低于对照组(P<0.05)。结论:中药汤剂灌肠治疗小儿发热的疗效较西医传统治疗退热效果好,并能降低血清中CRP的水平,抑制由CRP引起的炎性级联反应,值得儿科临床推广应用。     

星点设计-效应面法优化硼酸乳膏基质配方及质量控制

摘 要 目的：优化硼酸乳膏基质配方,考察制剂成品质量。方法: 采用星点设计效应面法,以乳膏黏度为评价指标,优化脂肪醇醚-6（和）硬脂醇、鲸蜡硬脂醇聚醚-25、十六醇、十八醇、异硬脂醇异硬脂酸酯、霍霍巴油、轻质液状石蜡的用量;考察优化后制剂的外观性状、粒度、黏度、稳定性及硼酸的含量。结果: 配方中含脂肪醇醚-6-鲸蜡硬脂醇聚醚-25（3∶2）50 g、十六醇-十八醇（3∶7）65 g、异硬脂醇异硬脂酸酯 霍霍巴油-轻质液状石蜡（5∶3∶2）125 g时,制得的成品为白色半流体乳膏,硼酸含量为标示量的98.50%,粘度为1.64×10⁴ mPa·s,预测值与实测值相当,制剂稳定性较好。结论:星点设计 效应面法优化硼酸乳膏基质配方方法简单、精密度高、预测性好,制剂成品稳定、质量可控。     

一种体液生物信息光纤动态检测系统的研制

目的：研制并介绍一种新型体液生物信息光纤动态检测系统.方法：采用光纤传感探头结构,通过留置于人体内或体外的较薄皮肤进行微血管内血液的参数或其他体液参数的采集、检测,得到光谱信息,并与预先储存的标准光谱信息参数进行比较,从而得出异于标准的体液成分.结果：该系统应用于临床,可对人体体液（包括血液）进行检测,获取患者体液较为全面的信息;同时,适用于微血管内血液参数的检测,了解患者产生病变的过程.结论：采用该系统可实时了解人体的病理、生理状态,对指导糖尿病等疾病的早期诊治提供重要信息.     

基于PI3K/AKT通路探究柚皮素改善多囊卵巢综合征大鼠胰岛素抵抗的作用机制

目的　基于磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（AKT）通路,初步探究柚皮素防治大鼠多囊卵巢综合征（PCOS）胰岛素抵抗的分子机制。方法　SD大鼠颈背部每日皮下注射脱氢表雄酮建立PCOS模型,将造模成功的大鼠采用随机数字表法分为模型组、柚皮素组、PI3K抑制剂组、柚皮素+PI3K抑制剂组,每组15只;相同时间段取SD大鼠15只,于颈背部皮下注射油剂2 mL/kg,作为正常对照组。检测各组大鼠血糖、胰岛素、血脂、生殖激素水平,计算胰岛素抵抗指数;HE染色观察卵巢形态;免疫组化法检测磷酸化PI3K（p-PI3K）阳性表达;Western blot法检测PI3K/AKT通路磷酸化蛋白及通路相关蛋白胰岛素受体底物-1（IRS-1）、糖原合成酶激酶-3β（GSK-3β）、葡萄糖转运蛋白因子-4（GLUT4）、人第10号染色体上磷酸酶和张力蛋白同源缺失基因（PTEN）蛋白表达。结果　与正常对照组相比,模型组大鼠卵巢组织出现卵泡囊性扩张、黄体数量及颗粒细胞层减少、闭锁卵泡增多等病理损伤症状,血脂、血糖、胰岛素水平升高,胰岛素抵抗增加,生殖激素分泌紊乱,PI3K/AKT通路磷酸化蛋白及通路相关蛋白IRS-1、GSK-3β磷酸化蛋白表达、GLUT4蛋白表达降低,PTEN蛋白表达升高（P＜0.05）。柚皮素可减轻卵泡囊性扩张等病理损伤,促进PI3K/AKT通路及通路相关蛋白IRS-1、GSK-3β磷酸化蛋白及GLUT4蛋白表达,改善生殖激素分泌紊乱现象,减轻胰岛素抵抗,降低血糖、血脂水平及PTEN蛋白表达（P＜0.05）。PI3K抑制剂可减弱柚皮素的上述作用（P＜0.05）。结论　柚皮素可通过促进PI3K/AKT通路活化,降低血糖、血脂水平及胰岛素抵抗,改善PCOS大鼠生殖激素紊乱及卵巢多囊改变。     

Retrospective study of effect of whole-body vibration training on balance and walking function in stroke patients

BACKGROUNDDysfunction in stroke patients has been a problem that we committed to solve and explore. Physical therapy has some effect to regain strength, balance, and coordination. However, it is not a complete cure, so we are trying to find more effective treatments.AIMTo observe the effect of whole-body vibration training (WVT) on the recovery of balance and walking function in stroke patients, which could provide us some useful evidence for planning rehabilitation.METHODSThe clinical data of 130 stroke participants who underwent conventional rehabilitation treatment in our hospital from January 2019 to August 2020 were retrospectively analyzed. The participants were divided into whole-body vibration training (WVT) group and non-WVT (NWVT) group according to whether they were given WVT. In the WVT group, routine rehabilitation therapy was combined with WVT by the Galileo Med L Plus vibration trainer at a frequency of 20 Hz and a vibration amplitude of 0+ACY-plusmn+ADs-5.2 mm, and in the NWVT group, routine rehabilitation therapy only was provided. The treatment course of the two groups was 4 wk. Before and after treatment, the Berg balance scale (BBS), 3 m timed up-and-go test (TUGT), the maximum walking speed test (MWS), and upper limb functional reaching (FR) test were performed.RESULTSAfter 4 wk training, in both groups, the BBS score and the FR distance respectively increased to a certain amount (WVT = 46.08 ± 3.41 vs NWVT = 40.22 ± 3.75; WVT = 20.48 ± 2.23 vs NWVT = 16.60 ± 2.82), with P < 0.05. Furthermore, in the WVT group, both BBS score and FR distance (BBS: 18.32 ± 2.18; FR: 10.00 ± 0.92) increased more than that in the NWVT group (BBS: 13.29 ± 1.66; FR: 6.16 ± 0.95), with P < 0.05. Meanwhile, in both groups, the TUGT and the MWS were improved after training (WVT = 32.64 ± 3.81 vs NWVT = 39.56 ± 3.68; WVT = 12.73 ± 2.26 vs NWVT = 15.04 ± 2.27, respectively), with P < 0.05. The change in the WVT group (TUGT: 17.49 ± 1.88; MWS: 6.79 ± 0.81) was greater than that in the NWVT group (TUGT: 10.76 ± 1.42; MWS: 4.84 ± 0.58), with P < 0.05.CONCLUSIONThe WVT could effectively improve the balance and walking function in stroke patients, which may be good for improving their quality of life.     

Observation on clinical efficacy of acupuncture combined with direct moxibustion with fine-strip moxa for 62 cases of herpes zoster

ObjectiveTo evaluate the clinical effect of acupuncture combined with direct moxibustion with fine-strip moxa for herpes zoster.MethodsSixty-two patients with herpes zoster were randomly divided into two groups according to attendance number, 32 in acupuncture group and 30 in western medicine group. The patients in the acupuncture group were treated by acupuncture at the nidus-related nerve segments Jiāj

(

EX-B2) in combination with direct moxibustion with fine-strip moxa, while the patients in the western medicine group were treated by oral valaciclovir hydrochloride, vitamin B₁, vitamin B₁₂. The times of response, incrustation and decrustation were observed respectively, and pain relief degree of the two groups were compared.ResultsThe response time, incrustation time and decrustation time of the acupuncture group were all less than those of the western medicine group [(1.74±0.43) days vs (3.86±0.58) days, (2.03±0.52) days vs (5.46±0.65) days, (5.14±0.33) days vs (8.34±0.59) days, all P<0.05]. The pain relief degree (VAS score), and pain duration in the acupuncture group were all higher obviously than those of the western medicine group [(10.41±12.1) vs (15.63±11.39), (4.78±0.45) days vs (8.12±0.63) days, all P<0.05]. The total effective rate of the acupuncture group was 96.9% (31/32) and that of the western medicine group was 90% (27/30), thus the therapeutic effect of acupuncture group was better than that of western medicine group (P<0.05).ConclusionThe therapeutic effect of acupuncture combined with direct moxibustion with fine-strip moxa is remarkable. It can effectively control the development of herpes zoster, alleviate pain and shorten therapy periods. It is worthy to be promoted and applied.     

山西汉族人群Y—STR基因座DYS714的多态性研究

目的研究山西汉族群体Y—STR基因座DYS714的多态性,并探讨其在法医学中的应用价值。方法应用PCR扩增方法结合DNA分析序列技术对120例山西地区汉族男性个体DYS714基因座进行分析。结果DYS714基因座在山西汉族男性个体中观察到5个等位基因,核心序列为ttttc,重复14—18次,基因频率分别为0．15、0．1583、0．2167、0．3333、0．1416,基因多样性为0．7808。个人识别能力（DP）和非父排除率（PE）均为0．7808。28组家系调查无突变。结论DYST14基因座具有很好的多态性和稳定性。