雷洛昔酚对大鼠垂体的作用

目的 观察雷洛昔酚是否能诱发出催乳素瘤的动物模型以及对PRL水平的影响,以研究雷洛昔酚对大鼠垂体的作用。方法雌性Wistar大鼠切除卵巢后,分别在皮下埋植含有雷洛昔酚、雌激素和空白硅胶管,术后8周处死大鼠,检测大鼠体重变化、垂体重量变化、血清催乳素(PRL)水平和垂体组织学变化。结果雷洛昔酚组与阴性对照组大鼠体重无明显统计学差异,与雌激素组大鼠体重具有统计学差异(P<0.05)；雷洛昔酚组与阴性对照组大鼠垂体重相比无明显差异,与雌激素组大鼠垂体重相比具有统计学差异(P<0.05)；雌激素组大鼠血清PRL水平最高,阴性对照组血清PRL水平最低,雷洛昔酚组介于两者之间,分别与雌激素组、对照组相比较差异均具有统计学意义(P<0.05)；雷洛昔酚组与对照组垂体病理为正常细胞形态,雌激素组垂体病理为PRL瘤表现。结论雷洛昔酚对大鼠垂体有一定的影响,但不能诱发催乳素瘤。     

精神疾病患者的体内25-(OH)维生素D的不足与缺乏

目的:了解精神疾病患者体内25-(OH)维生素D不足与缺乏的患病率,为合理的维生素D治疗提供依据。方法:用化学发光免疫法定量测定精神分裂症、血管性痴呆和脑器质性疾病伴精神障碍三类人群及健康体检者血清中的25-(OH)维生素D的含量,比较其均值。结果:与对照组相比,三组病人血清中25-(OH)维生素D的含量均低,但精神分裂组和血管性痴呆组偏低程度有显著性差异。结论:维生素D在精神疾病人群中含量较正常人低,提示给予合适的维生素D治疗,可能能促进病情的好转。     

Potential role of ATP-binding cassette transporters in the intestinal transport of rhein

Rhein, a lipophilic anthraquinone, exhibits anti-inflammatory and anti-tumor activities; however, it is hepatotoxic. ATP-binding cassette transporters, including P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance-associated protein 2 (MRP2), can pump toxicants from gut epithelial cells back into the intestinal lumen to prevent poisoning. We investigated their roles in rhein transport using a rat intestinal perfusion model and Caco-2, MDCKII-MDR1 (high expression of P-gp), MDCKII-BCRP (high expression of BCRP) and MDCKII-MRP2 (high expression of MRP2) cell models. The permeability of rhein in the duodenum significantly increased with increasing perfused concentration of rhein in the rat model, suggesting that efflux transporters were involved in rhein transport. In the Caco-2 cells, the permeability of rhein from the basolateral (B) to the apical (A) was significantly higher than that from A to B. In the presence of BCRP or MRP2 inhibitor, the permeability of rhein significantly decreased from B to A direction. In the MDCKII-BCRP cells, rhein was more permeable in B to A side than that in the opposite side. However, no significant differences of rhein permeability were observed in two directions in both MDCKII-MDR1 and MDCKII-MRP2 cells. Taken together, these results suggested that only BCRP was involved in rhein transport.     

混合型生物人工肝治疗设备的研制

目的:结合临床应用需求,研制一种新式混合型生物人工肝治疗应用系统。方法:以2个可编程控制器(PLC)作为控制系统核心,辅以多种安全监测报警装置,具有密闭、洁净和恒温的生物反应治疗环境,为离线混合型生物人工肝治疗提供一体化集成解决方案。结果:该设备能实现对血液流量、流速、质量等参数的精确控制,并提供温度、气泡、漏血等参数的报警监测功能。控制系统操作简便,人机界面友好,并为封闭式生物反应离线治疗过程提供实时影像监控。结论:新型治疗设备实现了混合式生物反应人工肝的离线治疗,有效缩短了生物人工肝治疗时间,同时改善了生物治疗环境。     

The downregulation of ANGPTL4 inhibits the migration and proliferation of tongue squamous cell carcinoma

ObjectiveTongue squamous cell carcinoma (TSCC) is the most common malignant cancer in the oral cavity, with a high rate of metastasis to the neck lymphoid node. Angiopoietin-like protein 4 (ANGPTL4) and microvessel density (MVD) may be novel indicators for tumor metastasis. The aim of the present study was to investigate the expression and function of ANGPTL4 in TSCC and the relationship between ANGPTL4 and MVD.MethodsThe expression levels of ANGPTL4 and MVD (CD34) were analyzed in 65 TSCC specimens and the adjacent non-cancerous tissues using immunohistochemistry (IHC). siRNA was delivered into TSCCA cells to downregulate ANGPTL4 expression. Subsequently, validation with real-time RT-PCR and western blot analyses was performed to analyze ANGPTL4 expression levels. In addition, a proliferation assay, migration and invasion assays were carried out.ResultsANGPTL4 expression was associated with tumor stage, lymph node metastasis and MVD expression. Cox regression analysis showed that high levels of ANGPTL4 expression were closely associated with poor survival time. In vitro analyses using qRT-PCR and western blot confirmed that ANGPTL4 was successfully inhibited in TSCCA cells. Suppressing ANGPTL4 resulted in the inhibition of cell proliferation and migration, but neither invasion nor cisplatin resistance was significantly affected.ConclusionHigh expression levels of ANGPTL4 are associated with the T stage, lymphatic metastasis, angiogenesis and poor overall survival in TSCC patients. The downregulation of ANGPTL4 inhibits the migration and proliferation of cells in TSCC. Taken together, ANGPTL4 may serve as a novel biomarker and therapeutic target for TSCC.     

酒精性肝纤维化基质降解机制的研究

目的：揭示酒精性肝病肝纤维化基质降解的病原机制。方法：28例酒精性肝炎肝穿刺组织按其纤维化程度分为3组，利用原位杂交技术以寡聚核苷酸为探针，分别检测各组MMP－1、MMP－2、MT1－MMP和TIMP－1 mRNA的表达情况。结果：发现MMP－1、MMP-2、MT1－MMP和TIMP－1 mRNA表达阳性的细胞主要是肝窦壁细胞（可能为增生的HSC）和少数肝细胞；MMP－2、MT1－MMP 和TIMP－1mRNA表达阳性细胞数随肝纤维化程度的增加而增多，相反，MMP－1 mRNA表达阳性细胞数则随肝纤维化程度的增加而减少。结论：HSC可能是肝组织内产生MMP－1、MMP－2、MT1－MMP和TIMP－1的主要细胞，在酒精性肝纤维化ECM沉积中起重要作用；MMP－1减少和TIMP－1增多可能是EMC沉积，尤其是I型胶原过量沉积的原因；而MMP－2和MT1－MMP增多的意义尚须进一步确定。     

2020年3—4月全球主要疫情回顾

本刊对2020年3—4月全球重点传染病疫情(截至4月28日)汇总如下。1新冠肺炎疫情肆虐全球,全球确诊病例数破300万新冠肺炎疫情自2019年底发生后,截至2020年2月底,全球病例数约8.3万例,进入3月以后,病例数呈指数增长。从2019年底确诊首例新冠肺炎病例到全球病例数达10万,共花了67 d时间,达到第2个10万仅用了11 d,达到第3个10万用了4 d,达到第4个10万用了3 d。     

酵母双杂交技术筛选与克隆白细胞中与NS5ATP7蛋白结合的蛋白编码基因

目的 应用酵母双杂交体系寻找与丙型肝炎病毒(HCV)非结构蛋白5A反式激活蛋白7(human gene 5 transactivated by nonstructural protein 5A of hepatitis C virus,NS5ATP7)相互作用的白细胞蛋白,以探讨SN5ATP7的生物功能。方法 应用酵母双杂交系统3,构建NS5ATP7诱饵质粒,转化酵母AH109,与含人白细胞cDNA文库质粒的酵母Y187进行配合,于涂有X-α-gal营养缺陷型培养基(SD／-Trp-Leu-His-Ade)上筛选生长。挑选蓝色克隆,提取此酵母克隆的质粒转化大肠杆菌提取质粒。DNA后进行测序,然后进行生物信息学分析。结果 筛选出15个与NS5ATP7特异性相互作用的克隆,其中包括人类RhoGDF分裂抑制剂α、人类细胞色素b558a亚单位、人类MLL(MLL)基因外显子、人类S100钙结合蛋白A9(钙粒蛋白B)、人类移动抑制因子相关蛋白14变体E(S100A9)、人类金属硫蛋白2A、人类染色体序列及人类cDNA序列等,1个是未知功能基因。结论 初步克隆了NSSATP7与白细胞结合蛋白基因,根据所克隆到的基因,对以后研究NS5ATP7的功能有一定的提示作用;为以后研究这些能与NS5ATP7相互作用的基因在白细胞中的生理功能奠定了基础。     

新媒体环境下医学图书馆服务水平的提升

介绍新媒体的内涵和特征,阐述新媒体在信息传播中的表现形式丰富、获取信息便捷等优势,论述新媒体环境下医学资源的采集与揭示,提出完善新媒体服务形式、理念的途径和方法。