性早熟女童行为问题及家庭因素的影响

目的探讨性早熟女童行为问题及家庭因素的影响。方法采用Achenbach儿童行为量表对6~10岁性早熟女童88例,同年龄未发育女童46例进行行为评定,采用家庭环境量表及自拟家庭及被测者一般情况调查表对家庭影响因素进行分析。结果性早熟女童行为问题明显多于对照女童,其行为问题检出率为35.2%,较对照女童(15%)显著升高(P<0.05);性早熟女童在抑郁、社交退缩、体诉、攻击性、内向因子、外向因子方面得分高于对照组,差异显著,在多动、残忍性方面得分亦高于对照组,存在差异;家庭环境的矛盾性、娱乐性对行为问题评分有影响,排除了家庭环境的矛盾性、娱乐性评分对行为问题总分的影响后,性早熟组行为问题总分仍高于对照组,两者差异有统计学意义;排除家庭经济文化因素的混杂作用,性早熟组发生行为异常的检出率亦高于对照组。结论性早熟女童出现行为问题比例较高,且与家庭环境有一定相关。     

磁共振水成像技术的临床应用

目的评价磁共振水成像技术(magnetic resonance hydrography,MRH)在临床诊断中的应用价值。方法回顾分析300例成功进行磁共振胰胆管、椎管和泌尿系水成像检查的病例,均采用重T2技术配合脂肪抑制及预饱和技术,使用感兴趣区重建技术进行三维重建,对三维图像做最大强度投影,进行多角度观察。结果随着检查目的不同,磁共振水成像技术对各器官的显示也有所不同。本组磁共振胰胆管、椎管和泌尿系水成像的阳性率分别为88.5%、74.3%和89.7%。结论磁共振水成像技术对胰胆管、椎管和泌尿系等含水器官的病变可清晰显示,通过对其形态的观察,可以对病变进行定位或定性的诊断,同时结合MR平扫及增强检查,诊断将更为准确。     

彩超和B-flow技术评估下肢深静脉瓣膜反流程度的临床价值

目的探讨彩超和B-flow技术在检测下肢深静脉瓣膜反流程度的应用价值。方法对85例下肢静脉病患者进行检测,其中肢体反流0度10条,Ⅰ度22条,Ⅱ度26条,Ⅲ度17条,Ⅳ度10条,测量股总静脉直径、股浅静脉直径、反流时间、反流速度、平静呼吸速度、反流指数,同期行静脉造影。结果反流指数(除0度与Ⅰ、Ⅱ度外)两两之间差异均有显著意义。反流时间:Ⅳ度、Ⅲ度分别与Ⅰ度、Ⅱ度间差异有显著意义。股浅静脉与股总静脉直径各度之间差异无显著性意义。结论超声可准确诊断下肢深静脉瓣膜功能不全,静脉反流指数是评价深静脉瓣膜功能不全反流程度的可靠指标。     

人乳头状瘤病毒感染型别与子宫颈上皮内瘤变的关系

目的采用聚合酶链反应(PCR)-寡核苷酸微流芯片进行人乳头状瘤病毒(HPV)分型,并研究子宫颈上皮内瘤变(CIN)与HPV感染型别的关系。方法对335例临床病例进行筛查、细胞学分级并分组,活检标本通过寡核苷酸微流芯片上特异性探针进行HPV分型。结果335例患者中,HPV阳性率为22．1%。宫颈良性疾病组的HPV阳性率为10．9%,CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌组分别为47．2%、66．7%、68．4%和75．0%,均显著高于宫颈良性疾病组(P值均＜0．05)。结论随着CIN级别的升高,高危HPV型感染比例增加,寡核苷酸微流芯片检测能区分多种HPV型别,可用于临床宫颈上皮内瘤变的HPV筛查。     

Relationship Between Parenting and Proactive Versus Reactive Aggression Among Chinese Preschool Children

This study examines the relationship between parenting and proactive versus reactive aggression among preschool children in China. Children (1164) from 10 kindergartens in Shanghai were rated by their parents and teachers using the Parent Behavior Inventory (PBI) and the Aggressive Behavior—Teacher's Checklist. Children had higher levels of reactive than proactive aggression, and older children and boys had higher levels of both proactive and reactive aggression. Hostile/coercive parenting style and low father education were significantly linked to aggression in children. These findings suggest that parenting style and type of aggression should be addressed when considering prevention and intervention.     

Effect of lentivirus-mediated gene silencing,targeting toll-like receptor 2, on corneal allograft transplantation in rats

AimThe present work aims to assess the effectiveness of lentiviral vector (LV) mediated Toll-like receptor 2 (TLR2) gene silencing in the survival of transplanted corneal allografts, against immune rejection, in rats.MethodsLV mediated TLR2 small interference RNA (SiRNA) with enhanced green fluorescent protein (eGFP) [LV-TLR2-siRNA-eGFP] was realised and transfected to both rat corneal epithelial (EC) and stromal cells (SC). Multiplicity of infection (MOI) was optimized for transfection efficiency using flow cytometric (FCM) analysis. Viability of transfected cells and the success rate of TLR2 gene silencing were respectively determined by CCK-8 assay and western blot assay. The in-vivo experiments were subjected to a penetrating keratoplasty (PK) performed between host Sprague Dawley (SD) and donor Wistar/SD rats, randomly dividing them into 4 groups including the allograft, isograft, allograft treated with LV-eGFP (LV blank control) and allograft treated with LV-TLR2-siRNA-eGFP (LV treated group). The rejection index (RI) was then recorded under a slit lamp, every day following surgery. Expression of the TLR2 and Myeloid differentiation primary response gene 88 (MyD88) were detected by using immunohistochemistry on day 9 post-surgery, whereas grafts’s TLR2 and MyD88 mRNA were determined on day 5, 9, and 14 post-surgery performing RT-PCR and, normal rat corneas were included as additional controls.ResultsTransfected cells showed the strongest eGFP expression when MOI was 200 with an efficiency of 77.5% for EC and 76.3% for SC. CCK-8 assay, as measured at 72 and 96 h post transfection, showed no significant changes in the cell viability (both EC and SC) between the transfected and the control group (p > 0.05, p > 0.05). Western blot results demonstrated a successful down regulation of TLR2 expression by LV-TLR2-SiRNA-eGFP, in both EC and SC. In vivo, compared to allograft group, LV treated group demonstrated less edema, opacity and neovascularization. Immunohistochemical analysis revealed a lower expression of TLR2 and MyD88 in isograft and LV treated group as compared to allograft group. TLR2 and MyD88 mRNA were detected in all grafts, and increased over time. With its highest expression in allograft group (peak on day 9), the mRNA expression for TLR2 and MyD88 showed a significant difference amongst the groups, on both day 9 and 14 (p < 0.001).ConclusionsLV mediated TLR2 siRNA could effectively down regulate the TLR2 expression via RNA interference and prolong the survival of corneal grafts, although not necessarily able to prevent the rejection.     

茵陈蒿汤经透皮给药治疗胆绞痛的研究

目的：为探索治疗胆绞痛新途径，采用中药经透皮给药系统（ＴＤＳ），并结合现代测试方法试图阐明机制。方法：茵陈蒿汤为主的１２味中药制成乳膏剂贴敷患者胆囊区；分成三组：（Ａ）胆绞痛急诊就医者３０例、（Ｂ）胆石症准备手术且有右上腹疼痛者２８例、（Ｃ）对照组２０例；除观察止痛疗效外，还采用薄层层析一分光光度法，检测尿液、胆汁、胆囊和膏药中的药用成份一对羟苯乙酮。结果：（１）疼痛症状明显缓解率７６％（４４／５８例），其中Ａ组８０％（２４／３０例），Ｂ组７１％（２０／２８例），二组差异无显著性（Ｐ＞０．０５）；（２）Ｂ组尿液、胆汁、胆囊和乳膏剂中的对羟苯乙酮含量分别为３９．０４±６．９９，７９．３４±２１．６９（ｍｍｏｌ．Ｌ－１）；０．４６±０．１０，１．８３７±０．２１（ｍｏｌ．ｋｇ－１），４种标本中的含量进行比较，差异均有非常显著性（Ｐ＜０．００１）。结论：茵陈等中药透过皮肤局部给药不仅能治疗胆绞痛，而且具有药物进入靶器官浓度明显高于全身的优越性，从而发挥较好的疗效。     

围生期生殖道支原体感染与胎儿窘迫关系的前瞻性研究

目的 探讨围生期生殖道支原体感染与胎儿窘迫的关系。方法 随机入选７３９名孕妇，采用支原体培养法检测围产期阴道解脲支原体和人型支原体，根据检测结果分为支原体阳性组和支原体阴性组，随访观察两组胎儿窘迫发生率。结果 支原体阳性组２４７例，支原体了性组４９２例，前者胎儿窘迫发生率５．３％（１３／２４７），后者２．０％（１０／４９２），前者显著高于后者（Ｐ〈０．０５）；队列研究显示孕期支原体感染显著增加胎儿     

Lentivirus介导RNAi抑制人胶质瘤干细胞STAT3基因生物学效应观察

目的了解慢病毒载体对胶质瘤干细胞的亲嗜性及对靶基因表达的干扰效率，初步探索干扰STAT3基因对胶质瘤干细胞增殖的影响。方法构建STAT3基因shRNA的慢病毒表达载体，经293T细胞包装后，获得可表达STAT3基因shRNA的慢病毒颗粒；活性载体病毒感染人原代胶质瘤干细胞后流式细胞分析细胞感染效率；实时定量多聚酶链反应（PCR）和Westernblot检测细胞STAT3基因mRNA和蛋白表达及活化水平；增殖分析试剂盒测定细胞生长曲线，流式细胞分析细胞周期分布。结果在病毒感染比率值20：1时慢病毒载体对人原代胶质瘤干细胞的感染效率为98．6％；细胞感染可表达STAT3基因shRNA的载体慢病毒后，STAT3基因mRNA显著下降，抑制率为84．3％，STAT3蛋白表达下降81．5％，活化的pSTAT3下降97．9％；胶质瘤干细胞STAT3表达、活化受抑后细胞生长显著变慢，G1期细胞比例显著增高。结论慢病毒载体对人原代胶质瘤干细胞有着很高的感染效率，介导的RNAi可显著抑制靶基因的表达与活化，是对人胶质瘤干细胞基因功能研究的理想工具。人胶质瘤干细胞STAT3基因受抑后细胞生长显著变慢，出现G1期阻滞。     

鳃裂畸形的临床分析

目的 深入了解鳃裂畸形的特点。方法 对85例鳃裂畸形进行分析，并结合文献进行探讨，结果鳃裂畸形的行烃比较，B超、CT、直接喉镜等可帮助其诊断，治疗以手术为主。结论 鳃裂畸形以手术切除为主，应注意鉴别鳃裂癌与转移至鳃裂囊肿附近的癌肿，无足够的语气表明鳃裂囊肿能够恶变。