端粒酶活性与妇科肿瘤

分析端粒活性与妇科肿瘤及其良恶性的相关性。方法用银染色端粒重复扩增法测定妇科肿瘤及正常组织中端粒酶活性。结果恶性肿瘤２１例,端粒酶阳性率９５．２４％,良性肿瘤１９例,阳性率３１．５８％;正常组织２０例,阳性率３０．００％。     

早期自然流产绒毛中胰岛素样生长因子2基因印迹状态研究

目的研究胰岛素样生长因子2(insulin like growth factor2,Igf2)基因的印迹状态及其表达在早期自然流产中的作用。方法选择2010年2月至2011年2月在中国医科大学附属盛京医院节育门诊就诊孕妇60例,采用PCR结合限制性片段长度多态性分析(restriction fragment length polymorphism,RFLP)技术,分析25例正常早孕、35例早孕自然流产绒毛组织中Igf2基因印迹丢失(loss of imprinting,LOI)情况;采用荧光定量PCR方法检测两种绒毛组织Igf2基因mRNA的表达。结果正常绒毛组织中Igf2基因型为杂合子的样本有11例,其中4例被检测出印迹丢失;自然流产绒毛组织中Igf2基因型为杂合子的样本有16例,其中6例被检测出印迹丢失,二者相比差异无统计学意义(P>0.05)。自然流产绒毛组织中Igf2基因mRNA的表达量为(2.29±0.60)×103copy/μgRNA,明显低于正常绒毛组织中Igf2基因的表达量(3.15±0.88)×103copy/μgRNA,两者比较差异有统计学意义(P<0.001)。结论 Igf2基因的印迹丢失与自然流产无关,Igf2基因mRNA的表达降低与自然流产有关,在早期自然流产发生中可能存在其他机制调节Igf2基因的表达。     

VIRAL PLACENTITIS - A CASE REPORT -

A case of viral placentitis, In a 20 year-old pregnant woman suffering from chronic glomerulonephritis, was presented. In light microscopic findings of the placenta, many necrotic foci with intranuclear Inclusion- bearing cells revealed in chorionic villi and by electron microscopy numerous immature and mature virus particles, probably herpes simplex were proved in intranuclear inclusion-bearing cells.     

Effect of blood transfusion during radiotherapy on the immune function of patients with cancer of the uterine cervix: role of interleukin-10

: To analyze prospectively the effects of blood transfusion administered during radiotherapy (RT) on the immune function of patients with locally advanced cervical cancer.

: In a total of 15 patients, 7 transfused and 8 untransfused, lymphocyte populations, including CD3+, CD4+, and CD8+ T-cell subsets, B cells (CD19+), and natural killer (NK) cells (CD56+, CD16+, CD3−) were studied before (i.e., time 0), during (i.e., times 1 and 2), and after (i.e., time 3) therapy. Expression of the early (CD25) and late (HLA-DR) activation markers on CD3+ T cells, the intracellular levels of perforin in CD8+ and CD56+ cells, and interferon (IFN)-γ, interleukin (IL)-2, and IL-4 in CD4+ and CD8+ T cells were also measured. NK cell cytotoxicity against the NK-sensitive target K-562 cells and CD8+ T-cell-directed cytotoxicity against OKT3 hybridoma cells were also assessed. Finally, the plasma levels of the immunoregulatory cytokine IL-10 were analyzed by enzyme-linked immunosorbent assay.

: The mean absolute number of all lymphocyte subsets compared with pretreatment levels decreased significantly during RT of both transfused and untransfused patients (p >0.001), with no detectable differences between the two groups in terms of total lymphocytes or relative numbers of CD3+ and CD4+ T cells, CD56+ NK cells, or CD19+ B cells. In contrast, concomitant with an inversion of the CD4/CD8 ratio, a significant increase in the number of CD8+ T cells at time 2 and CD3+ T cells, CD8+ T cells, and NK cells at time 3 was found in the transfused patients compared with the untransfused group. The percentages of CD25+/CD3+ T cells and HLA-DR+/CD3+ T cells increased during RT of the untransfused patients, but CD3+ T cells showed decreased CD25 expression and increased HLA-DR expression in the transfused group. An increase of CD8+ IFN-γ+ T cells with a concomitant decrease in CD8+ IL-2+ T cells was found in the transfused vs. untransfused group, and no differences were noted in the percentage of CD4+ IFN-γ+ T cells and CD4+ IL-2+ T cells. The proportion of perforin-positive CD8+ and CD56+ cells was higher in the transfused group than in the untransfused group. However, CD56+ cells and CD8+ T cells from the transfused patients showed markedly diminished cytotoxic function. Finally, IL-10 was detected only in the plasma of the transfused patients.

: Blood transfusion during primary RT for cervical cancer profoundly alters the magnitude and characteristics of radiation-induced immunosuppression. Elevated serum IL-10 in transfused patients may play a role in the disregulation of lymphocyte function, in particular, the depression of NK- and T-cell cytotoxicity. Investigation of alternatives to blood transfusion during RT that do not diminish host immunity is warranted.     

植物雌激素两种单体对子宫内膜腺上皮细胞作用的比较

目的 探讨植物雌激素两种单体在浓度5～80μmol／L对人子宫内膜腺上皮细胞增殖的影响。方法 2003年8～12月应用胶原酶阶梯消化法，原代培养人子宫内膜腺上皮细胞，用MTT方法观察植物雌激素的主要成分金雀黄素和大豆甙元对细胞体外增殖的影响。结果 低浓度的大豆甙元能促进子宫内膜腺上皮细胞轻度生长．随浓度的增加其作用明显，但当浓度达到40μmol／L，抑制细胞生长；不同浓度的金雀黄素均抑制子宫内膜腺上皮细胞的增殖。结论 植物雌激素两种单体对人子宫内膜腺上皮细胞的作用是明显不同的，提示在应用植物雌激素时要考虑不同成分的差别?     

沙眼衣原体感染输卵管性不孕患者输卵管液中肿瘤坏死因子α和白细胞介素6的测定

目的　探讨沙眼衣原体 (CT)感染所致输卵管性不孕患者输卵管液中肿瘤坏死因子α(TNF α)和白细胞介素 6 (IL 6 )的水平及其意义。方法　用酶联免疫吸附试验法测定 2 2例CT感染输卵管性不孕 (A组 )、2 3例非CT感染输卵管性不孕 (B组 )及 19例已生育输卵管正常妇女 (对照组 )输卵管液中TNF α和IL 6的水平。结果　A组TNF α的水平为 178ng/L ,高于对照组的 12 4ng/L(P<0 .0 1) ;A组中输卵管阻塞者TNF α为 199ng/L ,高于粘连者的 142ng/L(P <0 .0 1)。A组IL 6的水平为 6 81ng/L ,高于B组的 2 6 4ng/L及对照组的 2 2 9ng/L(P均 <0 .0 1) ,B组与对照组之间比较差异无显著性 (P >0 .0 5 )。结论　无症状输卵管CT感染可使输卵管液中TNF α和IL 6升高 ,其中TNF α与输卵管损伤程度有关。TNF α越高 ,损伤越重     

妊高征患者胎盘组织中FasL的表达及其意义

目的　探讨FasL在妊高征患者胎盘组织中的表达是否异常 ,进一步从免疫学角度分析妊高征的发病机制。方法　 2 0 0 1年 11月至 2 0 0 2年 7月 ,采用免疫组织化学SP法检测 2 0例正常孕妇 (对照组 ) ,6 5例妊高征患者 (妊高征组 ,其中轻度 2 2例、中度 2 0例、重度 2 3例 )胎盘组织中FasL表达强度。结果　FasL主要表达于胎盘绒毛滋养细胞 ,妊高征组胎盘绒毛滋养细胞及蜕膜组织细胞FasL表达强度明显低于对照组 (P <0 0 1,P <0 0 5 )。结论　妊高征患者胎盘组织FasL表达减少 ,母胎免疫耐受机制遭到破坏 ,引发胎盘绒毛发育不全、功能下降等一系列免疫病理改变 ,最终可能导致妊高征及其并发症的发生。     

妊娠期糖尿病母儿血清瘦素水平及胎盘瘦素的表达

目的　探讨妊娠期糖尿病 (gestationaldiabetesmellitus ,GDM)母儿血清瘦素水平及胎盘瘦素表达的变化。　方法　采用放射免疫法 (RIA)检测 2 4例GDM母儿和 2 6例正常母儿血清胰岛素和瘦素水平 ,采用逆转录 聚合酶链反应 (RT PCR)技术检测胎盘瘦素mRNA的表达水平。　结果　 (1)GDM组孕妇血清瘦素和胰岛素水平分别为 (18.6 2± 7.86 ) μg/L和 (13.4 7± 5 .11)mIU/L ,对照组分别为 (14 .2 1± 7.5 9) μg/L和 (8.98± 4 .2 3)mIU/L ,两组比较差异有显著性 (P <0 .0 5 )。 (2 )GDM组脐血瘦素和胰岛素水平分别为 (17.93± 6 .14 ) μg/L和 (19.2 6± 6 .73)mIU/L ,对照组分别为 (7.2 7± 4 .32 ) μg/L和 (9.6 7± 4 .89)mIU/L ,两组比较差异有显著性 (P <0 .0 1)。 (3)两组孕妇血清瘦素与胰岛素水平之间无相关关系 (r =0 .2 2 ,P >0 .0 5 ) ,而脐血血清瘦素与胰岛素水平之间呈正相关关系(r =0 .5 3,P <0 .0 1)。 (4 )GDM组胎盘瘦素mRNA表达水平为 (1.92± 0 .0 5 ) ,对照组为 (0 .97± 0 .0 2 ) ,两组比较差异有显著性 (P <0 .0 1)。　结论　胰岛素及瘦素抵抗可能是妊娠期糖尿病的发病原因之一 ,胰岛素对调节胎盘瘦素的表达起重要作用。     

胸腺基质淋巴细胞生成素基因多态性与子痫前期的相关性研究

目的 探讨胸腺基质淋巴细胞生成素（TSLP）位点rs1898671、rs2289278,rs2289276及rs764916基因多态性与妊娠妇女子痫前期发生的相关性。方法 收集2012年11月至2013年6月中国医科大学附属盛京医院分娩的孕妇384例。其中病例组181例,对照组203例,病例组又分为子痫前期轻度组（97例）和子痫前期重度组（84例）。选取TSLP4个位点（rs1898671,rs2289276, rs2289278 and rs764916）,利用基质辅助激光解析电离飞行时间质谱（MALDI-TOF MS）技术分析该基因的4个位点。结果 各临床资料在病例组与对照组比较中差异均有统计学意义（P＜0.05）,子痫前期轻度组与对照组比较中除年龄外其他各临床资料比较,差异均有统计学意义（P＜0.05）,子痫前期重度组与对照组以及子痫前期轻度组比较中各临床资料差异均有统计学意义（P＜0.05）。位点rs1898671等位基因频率在病例组与对照组中差异有统计学意义（P=0.043, OR=2.682, 95% CI 1.020～7.054）。结论 TSLP位点rs1898671基因多态性与子痫前期的发病可能具有相关性。等位基因T为子痫前期的危险性等位基因。     

Insulin receptor binding to monocytes and erythrocytes during normal human pregnancy

In normal human pregnancy glucose tolerance deteriorates gradually in spite of a steady increase in plasma insulin levels. To see whether this change in insulin resistance is accompanied by changes in insulin receptor binding, insulin binding to monocytes and erythrocytes was measured serially during pregnancy and again post-partum in fifteen normal women. Insulin binding to monocytes increased from week 12 to week 24 of gestation (P less than 0.001) and it decreased from week 32 to week 36 (P less than 0.05). After delivery a new increase in insulin binding to monocytes was seen (P less than 0.05). Insulin binding to erythrocytes increased from week 30-32 to week 36 (P less than 0.05), decreased from week 36 to delivery (P less than 0.01) and decreased further post-partum (P less than 0.001). Insulin receptor binding was not significantly correlated to plasma insulin, estradiol, estriol, progesterone or cortisol. The insulin receptor binding to monocytes, but not to erythrocytes, paralleled the insulin resistance found in human pregnancy.