Identification of a new lamin A/C mutation in a chinese family affected with atrioventricular block as the prominent phenotype

Even though mutations in LMNA have been reported in patients with typical dilated cardio-myopathy(DCM)and atrioventricular block(AVB)previously,the purpose of this study was to disclose this novel genetic abnormality in one Chinese family with the atypical phenotype of progressive AVB followed by DCM with normal QRS interval.Genome-wide linkage analysis mapped the AVB gene in this family to a marker at chromosome 1q21.2,where the LMNA gene was located.Direct DNA sequence analysis revealed a heterozygous G t...     

分布式家庭医生服务系统与区块链电子病历

对比传统集中式系统、分布式系统优缺点,指出分布式架构可解决传统架构存在的模块之间耦合度高、开发效率低等问题,设计构建分布式家庭医生服务系统,包括系统功能需求、体系架构、功能架构、分布式服务框架、服务调用流程等,同时在该系统中开发区块链电子病历,该系统及电子病历均通过测试。     

黄酮类化合物生物合成途径的进化及其在淫羊藿中的研究展望

黄酮类化合物是植物最重要的次级代谢产物之一。大多数植物黄酮类化合物的生物合成具有相同的步骤,其合成途径是植物进化过程中的保守代谢途径。迄今已经克隆、鉴定了多种植物黄酮类化合物生物合成相关酶的结构基因,并进行了一些黄酮类化合物合成代谢的分子调控研究。结合本实验室研究实践,综述了植物黄酮类化合物生物合成途径的进化研究进展,并在此基础上初步比较研究了淫羊藿与其他植物的黄酮类化合物合成途径,提出了研究淫羊藿黄酮类化合物生物合成的比较基因组学和比较代谢组学方法,指出应当从最保守的代谢中间产物和结构酶入手,结合淫羊藿种间和种内黄酮类化合物水平的比较,研究淫羊藿黄酮类化合物的代谢途径及其调控机制。     

Dynamic programming in parallel boundary detection with application to ultrasound intima-media segmentation

Segmentation of carotid artery intima-media in longitudinal ultrasound images for measuring its thickness to predict cardiovascular diseases can be simplified as detecting two nearly parallel boundaries within a certain distance range, when plaque with irregular shapes is not considered. In this paper, we improve the implementation of two dynamic programming (DP) based approaches to parallel boundary detection, dual dynamic programming (DDP) and piecewise linear dual dynamic programming (PL-DDP). Then, a novel DP based approach, dual line detection (DLD), which translates the original 2-D curve position to a 4-D parameter space representing two line segments in a local image segment, is proposed to solve the problem while maintaining efficiency and rotation invariance. To apply the DLD to ultrasound intima-media segmentation, it is imbedded in a framework that employs an edge map obtained from multiplication of the responses of two edge detectors with different scales and a coupled snake model that simultaneously deforms the two contours for maintaining parallelism. The experimental results on synthetic images and carotid arteries of clinical ultrasound images indicate improved performance of the proposed DLD compared to DDP and PL-DDP, with respect to accuracy and efficiency.     

HPV-DNA与TCT联合检测相关性分析

目的分析HPV-DNA、液基薄层细胞学检查(TCT)在宫颈癌检测中的相互作用。方法对从2009年9月到2010年4月检测的537例标本进行HPV-DNA与TCT联合检测。结果 2009年9月至2010年4月检测的537例标本中HPV阳性标本74例,其中TCT异常17例,异常率为23.0%;HPV阴性标本463例,其中TCT异常58例,异常率为12.5%。结论 HPV感染者中TCT异常者较多,感染者需重视TCT检测结果,定期检查以防宫颈病变恶化。     

Gap junctional coupling in the vertebrate retina: Variations on one theme?

Gap junctions connect cells in the bodies of all multicellular organisms, forming either homologous or heterologous (i.e. established between identical or different cell types, respectively) cell-to-cell contacts by utilizing identical (homotypic) or different (heterotypic) connexin protein subunits. Gap junctions in the nervous system serve electrical signaling between neurons, thus they are also called electrical synapses. Such electrical synapses are particularly abundant in the vertebrate retina where they are specialized to form links between neurons as well as glial cells. In this article, we summarize recent findings on retinal cell-to-cell coupling in different vertebrates and identify general features in the light of the evergrowing body of data. In particular, we describe and discuss tracer coupling patterns, connexin proteins, junctional conductances and modulatory processes. This multispecies comparison serves to point out that most features are remarkably conserved across the vertebrate classes, including (i) the cell types connected via electrical synapses; (ii) the connexin makeup and the conductance of each cell-to-cell contact; (iii) the probable function of each gap junction in retinal circuitry; (iv) the fact that gap junctions underlie both electrical and/or tracer coupling between glial cells. These pan-vertebrate features thus demonstrate that retinal gap junctions have changed little during the over 500 million years of vertebrate evolution. Therefore, the fundamental architecture of electrically coupled retinal circuits seems as old as the retina itself, indicating that gap junctions deeply incorporated in retinal wiring from the very beginning of the eye formation of vertebrates. In addition to hard wiring provided by fast synaptic transmitter-releasing neurons and soft wiring contributed by peptidergic, aminergic and purinergic systems, electrical coupling may serve as the ‘skeleton’ of lateral processing, enabling important functions such as signal averaging and synchronization.     

茯苓诱引栽培技术研究

目的：探索茯苓定点培育的新型栽培技术。方法：在使用传统“菌种”栽培茯苓的过程中，当茯苓菌丝体生长到一定阶段，再补充植入一块新鲜的幼菌核块（称为诱引），以其为“基核”诱导周围菌丝体进行聚集、纽结，进而形成个体较大的菌核。结果：诱引栽培试验与传统栽培试验比较，每窖形成菌核（结苓）数量由平均3个减少为1个；平均单产由1kg（干品）提高到1．3kg，增产约30％；药材商品成品产出率由80％提高到90％，增长约10％；药材主要成分含量相似，基本达到了定点培育的目的。结论：诱引栽培具有定点培育、便于管理、产量较高、质量稳定等优势，是值得大力推广的一项新型栽培技术。     

HPLC法测定云南不同产地白花丹的不同部位中白花丹醌的含量

目的:建立白花丹药材中白花丹醌的反相高效液相色谱测定方法,以分别考察云南不同产地白花丹药材的不同部位中白花丹醌的含量。方法:色谱柱为 Kromasil C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(70:30),流速为1.0mL·min~(-1),检测波长为254 nm,柱温为25℃。结果:白花丹醌在25.3～253μg·mL~(-1)范围内,具有良好的线性关系,r=0.9998;平均回收率为99.0%,RSD=1.4%(n=6)。不同产地白花丹药材地上部分的白花丹醌含量分别为0.046%(嘎洒),0.079%(勐养),0.134%(嘎东),0.032%(曼东),0.047%(曼阁),0.057%(曼老);根部的白花丹醌含量分别为0.842%(嘎洒),1.04%(勐养),1.44%(嘎东),0.763%(曼东),0.639%(曼阁),1.97%(曼老)。结论:本法简便、准确,灵敏度高,重复性好,可用于控制白花丹药材质量。