Human Exposure to Live Poultry and Psychological and Behavioral Responses to Influenza A(H7N9), China

To investigate human exposure to live poultry and changes in risk perception and behavior after the April 2013 influenza A(H7N9) outbreak in China, we surveyed 2,504 urban residents in 5 cities and 1,227 rural residents in 4 provinces and found that perceived risk for influenza A(H7N9) was low. The highest rate of exposure to live poultry was reported in Guangzhou, where 47% of those surveyed reported visiting a live poultry market >1 times in the previous year. Most (77%) urban respondents reported that they visited live markets less often after influenza A(H7N9) cases were first identified in China in March 2013, but only 30% supported permanent closure of the markets to control the epidemic. In rural areas, 48% of respondents reported that they raised backyard poultry. Exposure to live commercial and private poultry is common in urban and rural China and remains a potential risk factor for human infection with novel influenza viruses.     

Progesterone affects sex differentiation and alters transcriptional of genes along circadian rhythm signaling and hypothalamic‐pituitary‐gonadal axes in juvenile Yellow River Carp (Cyprinus carpio var.)

Progesterone (P4) is a biologically active steroid hormone that is involved in the regulation of oocyte growth and maturation, as well as development of the endometrium and implantation in the uterus of humans. It can also stimulate oocyte maturation in female fish, as well as spermatogenesis and sperm motility in male fish. Thus, P4 has been extensively used in human and animal husbandry as a typical progestin. However, P4 remaining in the water environment will pose a potential hazard to aquatic organisms. For example, it can interfere with sex differentiation and reproduction in aquatic vertebrates such as fish. Therefore, we investigated the effects of prolonged progesterone exposure on the expression of genes related to circadian rhythm signaling and the hypothalamic‐pituitary‐gonadal (HPG) axes in Yellow River Carp, which may have a potential impact on their sex differentiation. Our results suggested that P4 exposure altered the expression of genes related to circadian rhythm signaling, which can lead to disorders in the endocrine system and regulate the HPG axes‐related activities. Furthermore, the expression of genes related to the HPG axes was also altered, which might affect gonadal development and the reproductive systems of Yellow River Carp. In addition, these changes may provide a plausible mechanism for the observed shifts in their sex ratio toward females.     

穴位埋线治疗便秘96例

目的观察穴位埋线疗法治疗便秘的临床疗效。方法穴位（足三里、天枢、大肠俞等）消毒麻醉后,将2号医用羊肠线穿入腰穿针,植入选定的穴位。结果观察96例便秘患者,5d后起效96例,（占100％）。三个月后随访显效以上88例（占91．7％）,符合治愈标准的71例（占78．7％）。结论穴位埋线可激发经络之气,改变人体内分泌及体内的神经体液平衡,从而有效的治疗便秘。方法简易而行,患者痛苦少,疗效可靠,易于在基层医院推广应用。     

lncRNA HULC在膀胱癌组织中的表达及其对5637细胞恶性生物学行为的影响

目的:探讨长链非编码RNA(lncRNA)HULC在膀胱癌组织中的表达及其与患者临床病理特征的关系,以及沉默HULC对膀胱癌5637细胞增殖、凋亡、迁移与侵袭的影响.方法:选取2014年6月至2017年12月郑州人民医院手术切除的102例膀胱癌患者的癌及癌旁组织标本,以及人膀胱癌细胞株5637和人正常膀胱上皮细胞株SV...     

宫腔镜联合自凝刀射频消融子宫内膜治疗围绝经期功能性子宫异常出血的临床疗效分析

目的探讨宫腔镜联合自凝刀射频消融子宫内膜治疗围绝经期子宫功能性异常出血的临床疗效。方法选取2015年2月至2017年2月广东药科大学附属第三医院妇科诊治的81例围绝经期子宫功能性异常出血患者作为研究对象。随机分为观察组与对照组,其中观察组41例,对照组40例。观察组采用宫腔镜联合自凝刀射频消融子宫内膜治疗,对照组在超声引导下采用自凝刀射频消融子宫内膜治疗。观察比较两组患者的有效率、子宫内膜厚度以及Kupperman评分、血清卵泡生成激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平变化情况等。结果观察组平均手术时间、术中出血量同对照组比较,差异无统计学意义(P>0.05);观察组闭经率(65.85%)高于对照组闭经率(42.50%),差异具有统计学意义(χ~2=13.252,P=0.018),观察组总有效率(95.12%)与对照组总有效率(95.00%)比较,差异无统计学意义(χ~2=4.386,P=0.221),但秩和检验,差异无统计学意义(χ~2=4.386,P=0.221);观察组子宫内膜厚度及Kupperman评分与对照组相比,差异具有统计学意义(P<0.05);观察组血清FSH、E2、LH水平较对照组降低更为显著,差异具有统计学意义(P<0.05)。结论采用宫腔镜联合自凝刀消融子宫内膜手术,治疗围绝经期功能性子宫异常出血具有安全有效,可保留患者子宫,能显著提升患者疗效,更为彻底地消融子宫内膜,改善患者更年期综合征,降低血清激素水平,改善患者血清激素紊乱。     

缺氧缺血性脑损伤新生大鼠脑干听觉诱发电位的动态变化

目的：探讨缺氧缺血性脑损伤新生大鼠脑干听觉诱发电位（BAEP）的动态变化。方法：30只出生13天的新生SD大鼠随机分为对照组和缺氧缺血组,每组15只。将出生后第13天新生大鼠结扎左侧颈总动脉后,置于含氧8％的低氧环境中3h建立缺氧缺血性脑损伤动物模型。出生后第14、20、27、37、47、57、67和77天检测大鼠的BAEP。结果：缺氧缺血组新生大鼠3h缺氧复氧1h后,表现为向左侧旋转,爬行时右侧后肢呈现拖步。出生后第14～47天缺氧缺血组大鼠BAEP波峰潜伏期（PL）和波峰间潜伏期（IPL）较对照组显著延长（P〈0．05或P〈0．01）,这些鼠的BAEP的PL及IPL均随年龄的增加而缩短,并且鼠可发育成熟。结论：缺氧缺血性脑损伤新生大鼠BAEP有显著的可逆性异常变化,其PL和IPL是动态监测与客观评价新生期缺氧缺血性脑损伤和听觉障碍的灵敏指标。     

采用TaqMan MGB探针定量检测HCV 1b基因型C区氨基酸70变异

目的 建立一种定量检测HCV 1b基因型C区氨基酸70位点主要野生株（Arg-CGG,70w）和变异株（GIn-CAG,70M）的方法.方法 设计一对分别针对70 W和70 M的TaqMan MGB探针,建立一种实时逆转录聚合酶链反应定量检测方法.验证方法的敏感性、特异性和准确性.结果 所设计的引物和探针具有良好的特异性,敏感性可达5拷贝/反应.进一步的克隆测序证实了新方法的可靠性.结论 建立了一套HCV 1b亚型C区氨基酸70变异的定量检测系统,具有较高的敏感性、特异性和准确性,为研究70 W和70 M在抗病毒治疗中的动态变化提供了技术手段.     

润滑素在肌骨系统损伤修复中的作用

润滑素在肌骨系统各组织如关节软骨、肌腱、滑膜等中广泛表达,其表达与肌骨系统组织结构以及病理状态紧密相关。润滑素在肌骨系统中表现出生物润滑、防止黏连、抑制滑膜细胞增殖和调节炎症等重要功能,对组织损伤修复有重要作用。总结润滑素的表达、调控、结构及其在肌骨系统损伤修复中的作用,为进一步探究润滑素在肌骨系统中的功能和机理以及为肌骨系统损伤的临床治疗提供理论依据。     

一位自杀博主遗言评论留言的主题分析

目的:描述社交媒体中负向情绪的表达,探讨其与浏览者自杀风险的关系。方法:选取抑郁症自杀者"走饭"遗言微博下的10582条留言,利用NVivo11 Pro软件,由1名资深社会工作者对留言进行理解,建立树状节点,根据留言所含情绪进行留言的编码分类,经由另1名社工进行复查,产生分歧的留言由心理咨询师进行裁定。利用N11软件词频分析功能,统计留言中高频出现的词语及其频率。结果:微博留言者中表述自己患有抑郁症者占9%;表达自杀意念者占14%;负向表达留言占77%。词频统计发现"想死"、"杀"、"死了"、"离开"、"自杀"为高频的自杀相关关键词。结论:高自杀风险者聚集在该微博留言区,大量表达负性情绪,可能通过情绪传染对浏览者自杀风险产生影响。