硫酸庆大霉素注射液的快速比色分析

本文应用二价铜离子与硫酸庆大霉素在碱性介质中形成有色复合物的原理[1],采用比色法在560nm波长处直接测定硫酸庆大霉素的含量。该法平均回收率为100.2±0.47%。CV=0.47%,测定结果满意。     

子宫输卵管声学造影的临床应用

对15例不孕时限8个月至4年的妇女,应用生理盐水在B型超声监测下行子宫输卵管通液术。认为子宫输卵管声学造影对输卵管通畅性检查是一种新型、安全和无创伤的诊断方法.并就其各种输卵管通畅检查方法的利弊作了初步的分析比较.证明了该方法是实用可行的,值得推广.     

Pharmacokinetic and pharmacodynamic studies with mitoxantrone in the treatment of patients with nasopharyngeal carcinoma.

The pharmacokinetics and pharmacodynamics of mitoxantrone were studied in 15 patients with advanced nasopharyngeal carcinoma (NPC) after single intravenous rapid infusion (12 to 14 mg/m2). Mitoxantrone plasma concentrations and urinary excretion were measured specifically with the use of a high-performance liquid chromatographic method with ultraviolet detection at 242 and 658 nm. The pharmacokinetic parameters are described adequately by a three-compartment model with a terminal half-life of 71.5 +/- 40.1 hours and a volume of distribution of 5037 +/- 2377 l. The total plasma clearance was 743 +/- 462 ml/minute, and the renal clearance was 18.8 +/- 8.49 ml/minute. Within 72 hours, 1.8 +/- 0.6% of the administration dose was excreted in urine as mitoxantrone parent compound. From the urinary excretion rate data, glomerular filtration and possible tubular reabsorption were the mechanisms involved in the urinary excretion of mitoxantrone. The values for unbound fraction (%) in plasma at time 0 and 5 minutes were 2.88 +/- 0.91% and 3.25 +/- 1.19%, with an average of 3.04 +/- 1.01%. The degree of protein binding of mitoxantrone was not affected by concentration (P greater than 0.05) in Chinese patients with NPC. The response rate for mitoxantrone was poor in this study. Clinical studies have demonstrated that mitoxantrone was generally well tolerated. Only very low incidences of nausea, vomiting, and alopecia were observed. The mild and rapidly reversible dose-limiting hematologic toxic effects have proven leukopenia. Although the toxicities reported here were tolerated for most patients, other combination regimens including mitoxantrone or other administration routes may be considered and need to be evaluated carefully.     

乳腺癌患者血中角蛋白mRNA基因的表达

目的 分析乳腺癌患者外周血液中角蛋白（CK19）mRNA基因的表达。方法 取20例经病理证实的乳腺癌患者外周血，用Tirizol法提取总RNA，然后进行RT－PCR荧光定量检测，并与正常人群进行对照。结果 正常人群外周血中无CK19表达，20例乳腺癌中有6例阳性表达，总阳性率为29．65％，其中单纯癌阳性率为30．7％（4／13），浸润性导管癌阳性率为28．6％（2／7）。结论 CK19可作为检测外周血循环癌细胞的标志物。     

顺丁烯二酸水溶液的热反应动力学

研究了不同温度、浓度条件下，顺丁烯二酸（顺酸）非催化反应网络，即顺酸可生成反丁烯二酸（反酸）和苹果酸，反酸与苹果酸存在可逆反应。建立了该反应网络的动力学模型。测定了不同反应条件下顺酸、反酸和苹果酸浓度随时间变化的规律，据此，动力学参数进行了估值。     

Identification of Leishmania donovani isolates from different kala-azar foci in China by kDNA hybridization.

kDNA sequence homology of Leishmania donovani isolates from three types of kala-azar foci in China were analyzed by using dot and Southern hybridization with biotin- and 32P-labelled probes. The results revealed kDNA sequence heterogeneity among Leishmania donovani isolates from the three kala-azar foci: sequence homology between isolates of hill and desert foci was higher than that between hill and plain foci isolates. The kDNA hybridization technique was also found to be specific and sensitive for direct identification of Leishmania in animal tissues. In a preliminary survey, kDNA hybridization of cutaneous tissue blots of 71 dogs from endemic regions showed a positive rate of 40.8%, and the rate of double positive cases (touch blot hybridization and bone marrow smear) reached 91.3%. The direct identification of Leishmania in tissues by kDNA hybridization seems to be a useful and convenient method for epidemiological study and clinical diagnosis, especially for species/strain characterization.     

替代神经吻合法治疗喉返神经麻痹的解剖学研究

通过对30例人喉神经、膈神经、颈袢的解剖观测及利用图象分析仪二维重建技术对相关神经的截面积、纤维数量的测量比较分析,结合有关实验研究资料,设计了在甲状软膏板后下方开窗,于甲杓肌神经起点处切断该神经,用颈袢主支与甲杓肌神经吻合,用膈神经与喉下神经吻合,同时重建声带的内收和外展功能,并探讨了其解剖和生理学基础,认为此手术方法治疗喉返神经麻痹比较理想和可行。     

Advanced Chinese NiTi alloy wire and clinical observations.

Chinese NiTi wire was studied on the bench with six other nickel-titanium-alloy wires. Bending and torsional tests were conducted and temperatures of phase transformation compared. The Chinese NiTi wire was found to have a low stiffness, high springback and constant bending and torsional moments on unloading, in a very large deformation region. It can produce a gentle, nearly constant force. These factors make it desirable for clinical application. Included in this paper are clinical observations of case selected from over 100 patients in current treatment with Chinese NiTi wires. Chinese NiTi wire reduced the leveling and alignment phase of treatment without discomfort to the patient. Chinese NiTi wire can be used in both children and adults.     

自发性蛛网膜下腔出血的循证医学证据

近几十年来，随着神经影像学和放射介入技术的发展，对自发性蛛网膜下腔出血的病因有了新的认识，诊断和治疗水平也有了长足进步。目前，针对自发性蛛网膜下腔出血的抗纤维蛋白溶解药物的临床应用、扩充血容量升高血压的治疗、动脉瘤性蛛网膜下腔出血手术时机的选择及血管内治疗等方面研究不断深入，我们既往对此病症的认识与新进展之间存在较大的差异。笔者简要介绍自发性蛛网膜下腔出血的病因、诊断、治疗及预防等方面的主要研究进展，     

51例黄磷烧伤临床分析

对５１例黄磷烧伤病人进行临床分析。结果表明：黄磷对人体毒性大，即使烧伤面积不大，亦可因磷中毒而丧。磷中毒易造成肾、肝等裨性器官的损害。硫酸铜用于烧伤创面用量过大易引起铜中毒致急性溶血性贫血，不可作为黄磷 伤的创面用工。