Vasodilator effects of C-type natriuretic peptide on cerebral arterioles in rats

The vasodilator effects of C-type natriuretic peptide (CNP) were investigated in isolated rat cerebral arterioles. CNP caused dose-dependent vasodilation, maximally by 10.0±2.2% at 10⁻⁶ M. The median effective concentration (EC₅₀) was 5.2×10⁻¹⁰ M. In contrast, atrial natriuretic peptide and B-type natriuretic peptide, other members of the natriuretic peptide family, produced little or no vasodilation. Pretreatment with methylene blue (10⁻⁴ M) abolished CNP-induced vasodilation, whereas pretreatment with N^G-monomethyl-

Full-size image

-arginine or indomethacin did not inhibit vasodilation. Thus, CNP is suggested to cause significant vasodilation in cerebral arterioles via a cyclic guanosine monophosphate-dependent mechanism. © 1997 Elsevier Science B.V. All rights reserved.     

Thermafil热熔牙胶、Vitapex临床常用根管充填材料微渗漏比较研究

目的 :对比根充糊剂 牙胶尖、Vitapex 牙胶尖、Thermafil热熔牙胶三种根管充填材料对离体人牙根管的密封性能。方法 :将 5 0个新鲜拔除的人单根管牙 ,在釉牙骨质界处切除牙冠。将所有牙根随机分为 5组 ,每组 10个。 3个实验组分别用上述三种材料充填根管 ;剩余 2组 ,一组根管预备后不进行充填 ,另一组不进行根管预备 ,也不进行根管充填。用印度墨水染色法对比三种根管充填材料对离体人牙根管的密封性能 ,体视显微镜下测量染色线长度并进行统计学分析。结果 :根充糊剂 牙胶尖组、Vitapex 牙胶尖组和Thermafil热熔牙胶组的平均染色线长度分别为 3 .3 6± 0 .42mm、2 .0 9± 0 .19mm、1.0 5± 0 .3 7mm。Vitapex糊剂 牙胶尖或Thermafil热熔牙胶进行根管充填 ,产生的根管封闭性明显优于用根充糊剂 牙胶尖组根管充填 (P <0 .0 5 ) ;而Vitapex糊剂 牙胶尖和Thermafil热熔牙胶比较时 ,统计学无显著性差异 (P >0 .0 5 )。结论 :Thermafil热熔牙胶的密封性能最强 ,Vitapex糊剂 牙胶尖次之 ,根充糊剂 牙胶尖最弱。     

舍格伦综合征唇腺组织的细胞凋亡观察

目的　通过观察舍格伦综合征唇腺组织细胞凋亡的变化 ,探讨细胞凋亡在舍格伦综合征中的作用。方法　用原位末端标记法检测 16例舍格伦综合征的唇腺病变组织中的凋亡细胞数 ,并以 4例正常唇腺作为对照。每例随机统计 5个高倍视野的凋亡细胞数 ,并求出平均值。结果　染色结果显示舍格伦综合征患者唇腺腺泡及导管上皮可见散在阳性凋亡细胞 ,平均值为 ( 8 0 2± 2 2 3 )个 /高倍视野 ,正常唇腺组织细胞中阳性凋亡细胞为 ( 1 12± 0 3 4)个 /高倍视野 ,两组间对比差异有显著性。 (P <0 .0 5 )。舍格伦综合征病例的唇腺组织中腺泡及导管上皮可见散在的阳性凋亡细胞 ,其数量显著多于正常对照组。结论　考虑细胞凋亡可能是舍格伦综合征涎腺组织破坏的重要原因。     

Serotonergic projections from the midbrain periaqueductal gray and nucleus raphe dorsalis to the nucleus parafasccicularis of the thalamus

By a double-labeling method combining the retrograde tracing of horseradish peroxidase and the immunocytochemical technique, serotonin-like immunoreactive neurons in the midbrain periaqueductal gray (PAG) and nucleus raphe dorsalis (DR) of the rat were observed to send projection fibers to the nucleus parafascicularis of the thalamus bilaterally with an ipsilateral dominance. These serotonin-containing projecting neurons were observed mainly at the middle-caudal levels of the ventrolateral subdivision of the PAG and less at the middle-rostral levels of the DR.     

内支架结合放疗治疗晚期食管癌18例临床分析

目的　通过被覆支架治疗晚期食管癌恶性狭窄 ,并行术后放射治疗 ,以提高生活质量 ,延长生存期。方法　晚期食管癌恶性食管狭窄 18例 ,狭窄直径均在 2 .5mm以下 ,其中 3例呈梗阻状 ,4例合并有食管瘘 ,1例出现肝部转移。经口置入镍钛合金被覆支架 2 0枚 ,于 2周后行外照射 ,肿瘤试剂照射剂量 (DT) :6 0～ 70Gy。结果　所有患者均一次顺利完成支架置入 ,患者进普食顺利 ,疗效满意。 2周后均顺利接受放射治疗 ,未出现并发症。经随访复查 ,支架未发生移位及出现再狭窄改变 ,食管通畅 ,患者进食顺利 ,生存期延长 ,平均 11个月。结论　被覆支架治疗晚期食管癌恶性食管狭窄及术后结合放疗 ,能有效地抑制肿瘤生长 ,提高生活质量 ,延长生存期 ,是治疗晚期食管癌患者的一种安全有效的治疗方法。     

Segmental hyperintensity on T1-weighted MRI of the liver: Indication of segmental cholestasis

The purpose of this study was to determine the relationship between segmental hyperintensity of the liver on T1-weighted images and segmental cholestasis in patients with obstructive jaundice. T1-weighted and T2-weighted MR images were obtained of 73 patients with obstructive jaundice caused by various diseases. Fat-suppressed T1-weighted images were also obtained of 10 patients. Eleven patients with segmental intra-hepatic bile duct dilatation (cholestasis) showed segmental hyperintensity on T1-weighted images and/or fat-suppressed T1-weighted images and no signal intensity difference on T2-weighted images. Sixty-two patients with widespread intrahepatic bile duct dilatation showed no intensity difference on T1-weighted and T2-weighted images (P < .01). Segmental hyperintensity on T1-weighted images was correlated with intrahepatic cholestasis.     

Day-Night Variation of Urine Volume in Parkinson's Disease

Abstract: Studies on the circadian rhythm of urine excretion in healthy men have demonstrated that the maximal urine flow occurs in the early afternoon and the minimal around midnight. In this study, an abnormality in the variation of urine volume was found in parkinsonian patients. Urine samples were collected during daytime (9:00–21:00) and nighttime (21:00–9:00). Fifteen healthy control subjects were examined and found to excrete 60% during the daytime and 40% during the nighttime of the total urine volume. Sixteen parkinsonian patients excreted 43% during the daytime and 57% during the nighttime. In contrast to the control subjects, the parkinsonian patients excreted a smaller volume of their urine during the daytime than during the nighttime. This finding might be related to the degeneration of dopaminergic and/or nondopaminergic neurons in the brain which control urinary excretion.     

单孔钻颅引流术治疗慢性硬膜下血肿80例临床分析

慢性硬膜下血肿（chronic subdural hematoma，CSDH）为受伤3W后出现硬膜下的血肿，是中老年患者轻微颅脑损伤后最为常见的颅内病变。约占颅内血肿的10％，起病隐匿，且临床表现无特征。虽然CSDH的治疗方法多种多样，但单孔钻颅闭式引流方法简单，操作容易，已成为神经外科医师普遍采用的方法。我们近3年来采用单孔钻颅闭式引流术治疗慢性硬膜下血肿80例，效果满意，现报告如下。     

VEGF在肺癌组织中的表达及临床意义

目的：探讨血管内皮生长因子在肺癌中的表达情况与肺癌病理生物学行为之间的关系。方法：采用免疫组化SP法检测47例肺癌组织和10正常肺组织中VEGF的表达水平。结果：肺癌组织中VEGF的表达明显高于正常肺组织(P＜0．005)，P53、VEGF表达与肺癌的分化程度、淋巴结转移及P—TNM分期密切相关(P＜0．05)，与患者的性别、年龄、肿瘤大小及组织类型无关(P＞0．05)。结论：检测肺癌组织中VEGF的表达水平有助于了解肿瘤的生物学行为，并可作为判断其预后的有价值指标。